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| 1 | Mechanism of antagonistic effects of Andrographis paniculata methanolic extract against Staphylococcus aureus显示文摘Objective: To investigate the effects of Andrographis paniculata(Burm.f.) Wall. Ex Nees(A. paniculata) on expressions and activities of catalase, superoxide dismutase and alkylhydroperoxide reductase C in Staphylococcus aureus(S. aureus) with respect to its survival in vitro. Methods: Antioxidative property of methanolic leaves extract of A. paniculata(0.06 mg/m L). Minimum inhibitory concentration(MIC) was determined by its ability to reduce hydrogen peroxide(H_2O_2) toxicity against S. aureus ATCC 25923(3.8×10~8 cfu/m L). Effects of the extract on expressions of kat A(encoding catalase), sod A and sod M [encoding superoxide dismutases(SODs)], and ahp C [encoding alkylhydroperoxide reductase C(AhpC)] in S. aureuswere determined by RT-q PCR and corresponding enzyme activity assays were performed. Nitroblue tetrazolium reduction(NBT) assay was performed to determine effects of the extract on intracellular and extracellular levels of O_2-in S. aureus. Results: Cells challenged with 7.5 mmol/L H_2O_2 showed 0% survival in 30 min whereas 25% survived after treatment with the extract and H_2O_2. Cells that were treated with the extract alone had 43% survival in the same exposure period. Expressions of sod A and sod M genes in extract-treated cells were lowered 0.8-fold and 0.7-fold, respectively with decrease in total SOD activity of 26.8 U compared to untreated cells, 32.4 U(P<0.05). In contrast, extract-treated S. aureus cells showed 3.3-fold increase in kat A expression with corresponding increase in catalase activity of 1.828 U compared to untreated cells which was 1.248 U,(P<0.05). More profoundly, ahp C expression was increased 61-fold in extract-treated cells,(P<0.05) with corresponding increase in Ahp C activity of 0.018 U compared to untreated cells, 0.012U,(P<0.05). Extract-treated cells had significantly lower intra-and extracellular O_2-levels with absorbance readings(A_(575nm)) of 0.340 and 0.524 compared to untreated cells which were 0.516 and 0.928(P<0.05), respectively. Conclusions: Taken together these results suggest that the low MIC of A. paniculatamethanolic leaves extract(0.06 mg/m L) reduce H_2O_2 toxicity and more importantly, was in itself effectively inhibitory against S. aureus. Further, our observations suggest that a probable mode of its inhibitory mechanism against S. aureus is by reducing total SOD activity through downregulation of sod A and sod M expressions. | Roslinah Mohamad Hussain Zayan Nabilah Rasyidah Abd.Razak Wan Mazlina Md Saad Maimunah Mustakim | 2017 | Asian Pacific Journal of Tropical Medicine2017，10，7： | 3 |
| 2 | Hepatitis E virus infection in work horses in Egypt 显示文摘 | Saad MD Hussein HA Bashandy MM etal | 2007 | In~ect Genet Evol2007，7，3： | 1 |
| 3 | Genetic froms of HIV lin theformer soviet Union dominate the epidemic in Azeraijan显示文摘 | Saad MD Aliev Q Botros BA | 2006 | AIDSRes Hum Retroviruses2006，22，8： | 1 |
| 4 | A nascent HIV type 1epidemic among injecting drug users in Kabul,Afghanistan is domi-nanted by complex AD recombinant strain, CRF35-AD 显示文摘 | Sanders-Buell E Saad MD Abed AM | 2007 | AIDSRes Hum Retroviruses2007，23，6： | 1 |
| 5 | Vertebroplasty and Kyphoplasty for the Palliation of Pain显示文摘 | Ali Shaibani MD Saad Ali | 2007 | Seminars in interventional ra- diology2007，24，： | 1 |
| 6 | Determination of synthetic phenolic antioxidants in food items using reversed-phase HPLC显示文摘 | Bahruddin Saad Yong Yek Sing Mohd Asri Nawi NoorHasani Hashim Abdussalam Salhin Mohamed Ali Muhammad Idiris Saleh Shaida Fariza Sulaiman Khairuddin Md Talib Kamarudzaman Ahmad | 2007 | Food Chemistry2007，，1： | 1 |
| 7 | A case of Immune thrembocytopenia associated with pulmonary tuberculosis 显示文摘 | Eguakhide Inegbenebor MD Nibal Saad MD Pornchai I_eelasin- jaroen MD | 2014 | Chest2014，146，4： | 1 |
| 8 | Simultaneous determination of preservatives (benzoic acid,sorbic acid,methylparaben and propylparaben) in foodstuffs using high-performance liquid chromatography显示文摘 | Bahruddin Saad Md Fazlul Bari | 2005 | Journal of Chromatography A2005，1073，： | 1 |
| 9 | HIV infection and associ- ated risk factors among long-distance truck drivers travelling through Azerbaijan 显示文摘 | Botros BA Aliyev QM Saad MD | 2009 | IntJSTDAIDS2009，20，7： | 1 |
| 10 | A case of Immune thrombocytopenia associated with pulmonary tuberculosis(MeetiugAbstracts) 显示文摘 | Eguakhide Inegbenebor MD Nibal Saad MD Pornchai Leelasin- jaroen MD | 2014 | Chest2014，146，4： | 1 |
| 11 | Evolutionary based maximum power point tracking technique using differential evolution algorithm显示文摘 | Mohammad Faridun Naim Tajuddin Shahrin Md Ayob Zainal Salam Mohd Sazli Saad | 2013 | Energy & Buildings2013，，： | 1 |
| 12 | Qinghai-like H5N1 from domestic cats,northern lraq显示文摘 | Yingst SL Saad MD Felt SA | | 0，，08： | 1 |
| 13 | Hepatitis E virus infection in work homes in Egypt显示文摘 | Saad MD Hussein HA Bashandy MM | 2007 | Infect Genet Evol2007，7，3： | 1 |
| 14 | Simultaneous determination of preservatives (benzoic acid,sorbic acid,methylparaben and propylparaben) in foodstuffs using high-performance liquid chromatography显示文摘 | Bahruddin Saad Md Fazlul Bari | 2005 | J Chromatogr A2005，1073，1： | 1 |
| 15 | Impaired exocrine pancreatic function in diabetics with diarrhea and peripheral neuropathy显示文摘 | Dr. Hussein EL Newihi MD Cornelius P. Dooley MD MRCPI Dr. Carlos Saad MD Dr. Janet Staples MD Adina Zeidler MD Jorge E. Valenzuela MD | 1988 | Digestive Diseases and Sciences1988，，6： | 1 |
| 16 | Hepatitis E virus Infection in Work Horses in Egypt显示文摘 | Saad MD Hussein HA Bashandy MM | 2007 | Infect Genet Evol2007，7，3： | 1 |
| 17 | Chemically modified sugarcane bagasse as a potentially low-cost biosorbent for dye removal显示文摘 | Saad S A Isa K Md Bahari R | 2010 | Desalina- tion2010，264，： | 1 |
| 18 | Simultaneous determina- tion of preservatives (benzoic acid , sorbic acid, meth- ylparaben and propylparaben) in foodstuffs using high-performance liquid chromatography显示文摘 | Bahruddin saad Md Fazlu Bari | 2005 | Journal of chroma- tography A2005，1073，： | 1 |
| 19 | Simultaneous determination of preservatives(benzoic acid, sorbic acid, methylparaben and propylparaben) in foodstuffs using high-performance liquid chromatography 显示文摘 | BAHRUDDIN SAAD MD FAZLUL BARI IDIRIS SACEH MUHAMMAD | 2005 | J Chromatogr A2005，1073，1： | 1 |
| 20 | Hepatitis E virus infection in work horses in Egypt显示文摘 | Hussein HA Bashandy MM | 2006 | Infect Genet Evol2006，，： | 1 |
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