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| 1 | Effect of Helicobacter pylori cdrA on interleukin-8 secretions and nuclear factor kappa B activation显示文摘AIM:To investigate genetic diversity of Helicobacter pylori (H.pylori) cell division-related gene A (cdrA) and its effect on the host response.METHODS:Inactivation of H.pylori cdrA,which is involved in cell division and morphological elongation,has a role in chronic persistent infections.Genetic property of H.pylori cdrA was evaluated using polymerase chain reaction and sequencing in 128 (77 American and 51 Japanese) clinical isolates obtained from 48 and 51 patients,respectively.Enzyme-linked immunosorbent assay was performed to measure interleukin-8 (IL-8) secretion with gastric biopsy specimens obtained from American patients colonized with cdrA-positive or-negative strains and AGS cells cocultured with wild-type HPK5 (cdrA-positive) or its derivative HPKT510 (cdrA-disruptant).Furthermore,the cytotoxin-associated gene A (cagA) status (translocation and phosphorylation) and kinetics of transcription factors [nuclear factor-kappa B (NF-κB) and inhibition kappa B] were investigated in AGS cells co-cultured with HPK5,HPKT510 and its derivative HPK5CA (cagA disruptant) by western blotting analysis with immunoprecipitation.RESULTS:Genetic diversity of the H.pylori cdrA gene demonstrated that the cdrA status segregated into two categories including four allele types,cdrA-positive (allele types;Ⅰand Ⅱ) and cdrA-negative (allele types;Ⅲ and Ⅳ) categories,respectively.Almost all Japanese isolates were cdrA-positive (Ⅰ:7.8% and Ⅱ:90.2%),whereas 16.9% of American isolates were cdrA-positive (Ⅱ) and 83.1% were cdrA-negative (Ⅲ:37.7% and Ⅳ:45.5%),indicating extended diversity of cdrA in individual American isolates.Comparison of each isolate from different regions (antrum and corpus) in the stomach of 29 Americans revealed that cdrA status was identical in both isolates from different regions in 17 cases.However,12 cases had a different cdrA allele and 6 of them exhibited a different cdrA category between two regions in the stomach.Furthermore,in 5 of the 6 cases possessing a different cdrA category,cdrA-negative isolate existed in the corpus,suggesting that cdrA-negative strain is more adaptable to colonization in the corpus.IL-8 secretions from AGS revealed that IL-8 levels induced by a cdrA-disrupted HPKT510 was significantly lower (P < 0.01) compared to wildtype HPK5:corresponding to 50%-60% of those of wild-type HPK5.These data coincided with in vivo data that an average value of IL-8 in biopsy specimens from cdrA-positive and cdrA-negative groups was 215.6 and 135.9 pg/mL,respectively.Western blotting analysis documented that HPKT510 had no effect on CagA translocation and phosphorylation,however,nuclear accumulation of NF-κB was lower by HPKT510 compared to HPK5.CONCLUSION:Colonization by a cdrA-negative or cdrA-dysfunctional strain resulted in decreased IL-8 production and repression of NF-κB,and hence,attenuate the host immunity leading to persistent infection. | Hiroaki Takeuchi Ya-Nan Zhang Dawn A Israel Richard M Peek Jr Mikio Kamioka Hideo Yanai Norihito Morimoto Tetsuro Sugiura | 2012 | World Journal of Gastroenterology2012，18，5： | 3 |
| 2 | Diacylglycerol production in a packed bed bioreactor显示文摘 | WATANABE T SUGIURA M SATO M | 2005 | Process Biochem2005，40，2： | 1 |
| 3 | Karyotype at diagnosis is the major prognostic factor predicting relapse-free survival for patients with Philadelphia chromosome-positive acute lymphoblastic leukemia treated with imatinib-combined chemotherapy显示文摘 | Yanada M Takeuchi J Sugiura I | 2008 | Haematologica2008，93，2： | 1 |
| 4 | Preparation of Monodispersed Solid Lipid Microspheres Using a Microchannel Emulsification Technique 显示文摘 | Sugiura S Nakajima M Tong J | 2000 | J Colloid Interface Sci2000，227，7： | 1 |
| 5 | Cord blood IgE against milk and egg antigens 显示文摘 | Furuhashi M Sugiura K Katsuinata Y | 1997 | Biol Neonate1997，72，： | 1 |
| 6 | A case of systemicaplasia cutis congenita: a newly recognized syndrome显示文摘 | Sugiura TI Kouwaki M Kiyosawa S | 2008 | Eur JPediatr2008，167，4： | 1 |
| 7 | Abnormal embryonic karyotype is the most frequent cause of recurrent miscarriage 显示文摘 | SUGIURA M OZAKI Y KATANO K | 2012 | Hum Reprod2012，27，8： | 1 |
| 8 | In vivo3D kine- matic changes in the cervical spine after laminoplasty for cervical spondylotic myelopathy 显示文摘 | Nagamoto Y Iwasaki M Sugiura T etal | 2014 | J Neurosurg Spine2014，21，3： | 1 |
| 9 | Oxidative stress decreases klotho expression in a mouse kidney cell line 显示文摘 | Mitobe M Yoshida T Sugiura H | 2005 | Nephron Exp Nephro12005，101，2： | 1 |
| 10 | Apparent protein digestibility and mineral availabilities in various feed ingredients for salmonid feeds显示文摘 | Sugiura S H Dong F M Rathbone C K | 1998 | Aquaculture1998，159，： | 1 |
| 11 | Solitary neurofibroma : an uncommon location显示文摘 | SUGIURA K SUGIRUA M HAYAKAWA R | 2004 | Br J Dermatol2004，43，6： | 1 |
| 12 | Dietary microbial phytase supplementation and the utilization of phosphorus,trace minerals and protein by rainbow trout and soybean meal-based diets显示文摘 | Labuadan J Dong F M | 2001 | Aquaculture Research2001，32，7： | 1 |
| 13 | Association between adult-onset Still's disease and interleukin-18 gene polymorphisms显示文摘 | SUGIURA T KAWAGUCHI Y HARIGAI M | 2002 | Genes Immun2002，3，7： | 1 |
| 14 | Oxidative and nitrative stress in bronchial asthma显示文摘 | Sugiura H Ichinose M | 2008 | Antioxidants & Redox Signaling2008，10，4： | 1 |
| 15 | The geomagnetic field at 1982 from DE-2 and other magnetic field data 显示文摘 | Langel R A Ridgway J R Sugiura M | 1988 | Journal of Geomagnetism and Geoelectricity1988，40，9： | 1 |
| 16 | Retrospective analysis of 1502 pa tients with facial fractures显示文摘 | lida S Kogo M Sugiura T | 2001 | Int J Oral Maxillo{ac Surg2001，30，4： | 1 |
| 17 | Histological Epstein-Barr virus-related lymph node lesion autoimmune disease-like clinicopathological findings aged and elderly palients: a Study of six cases 显示文摘 | Kojima M Sugiura I hoh H | 2006 | Pratt2006，202，8： | 1 |
| 18 | Associa- tion between adult-onset Still' s disease and interleukin-18 gene polymorphisms 显示文摘 | SUGIURA T KAWAGUCHI Y HARIGAI M | 2002 | Genes Immun2002，3，7： | 1 |
| 19 | A randomised control study of partial liquid ventilation after airway lavage with exogenous surfactant in a meconium aspiration syndrome animal model 显示文摘 | NAKAMURA T MATSUZAWA S SUGIURA M | 2000 | Arch Dis Child Fetal Neonatal Ed2000，82，2： | 1 |
| 20 | Effects of superoxide dismutase on the acetycholine-induced relaxation response in cholesterol-fed and streptozotocin-induced diabetic mice显示文摘 | Kamata K Nakajima M Sugiura M | 1999 | J Smooth Musde Res1999，35，2： | 1 |
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