|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Changes in the colon microbiota and intestinal cytokine gene expression following minimal intestinal surgery显示文摘AIM: To investigate the impact of minor abdominal surgery on the caecal microbial population and on markers of gut inflammation.METHODS: Four week old piglets were randomly allocated to a no-surgery 'control' group(n = 6) or a 'transection surgery' group(n = 5).During the transection surgery procedure, a conventional midline incision of the lower abdominal wall was made and the small intestine was transected at a site 225 cm proximal to the ileocaecal valve, a 2 cm segment was removed and the intestine was re-anastomosed.Piglets received a polymeric infant formula diet throughout the study period and were sacrificed at two weeks post-surgery.Clinical outcomes including weight, stool consistency and presence of stool fat globules were monitored.High throughput DNA sequencing of colonic content was used to detect surgery-relateddisturbances in microbial composition at phylum, family and genus level.Diversity and richness estimates were calculated for the control and minor surgery groups.As disturbances in the gut microbial community are linked to inflammation we compared the gene expression of key inflammatory cytokines(TNF, IL1 B, IL18, IL12, IL8, IL6 and IL10) in ileum, terminal ileum and colon mucosal extracts obtained from control and abdominal surgery groups at two weeks post-surgery.RESULTS: Changes in the relative abundance of bacterial species at family and genus level were confined to bacterial members of the Proteobacteria and Bacteroidetes phyla.Family level compositional shifts included a reduction in the relative abundance of Enterobacteriaceae(22.95 ± 5.27 vs 2.07 ± 0.72, P < 0.01), Bacteroidaceae(2.54 ± 0.56 vs 0.86 ± 0.43, P < 0.05) and Rhodospirillaceae(0.40 ± 0.14 vs 0.00 ± 0.00, P < 0.05) following transection surgery.Similarly, at the genus level, changes associated with transection surgery were restricted to members of the Proteobacteria and Bacteroidetes phyla and included decreased relative abundance of Enterobacteriaceae(29.20 ± 6.74 vs 2.88 ± 1.08, P < 0.01), Alistipes(4.82 ± 1.73 vs 0.18 ± 0.13, P < 0.05) and Thalassospira(0.53 ± 0.19 vs 0.00 ± 0.00, P < 0.05).Surgeryassociated microbial dysbiosis was accompanied by increased gene expression of markers of inflammation.Within the ileum IL6 expression was decreased(4.46 ± 1.60 vs 0.24 ± 0.06, P < 0.05) following transection surgery.In the terminal ileum, gene expression of TNF was decreased(1.51 ± 0.13 vs 0.80 ± 0.16, P < 0.01) and IL18(1.21 ± 0.18 vs 2.13 ± 0.24, P < 0.01), IL12(1.04 ± 0.16 vs 1.82 ± 0.32, P < 0.05) and IL10(1.04 ± 0.06 vs 1.43 ± 0.09, P < 0.01) gene expression increased following transection surgery.Within the colon, IL12(0.72 ± 0.13 vs 1.78 ± 0.28, P < 0.01) and IL10(0.98 ± 0.02 vs 1.95 ± 0.14, P < 0.01) gene expression were increased following transection surgery.CONCLUSION: This study suggests that minor abdominal surgery in infants, results in long-term alteration of the colonic microbial composition and persistent gastrointestinal inflammation. | Susan Lapthorne Julie E Bines Fiona Fouhy Nicole L Dellios Guineva Wilson Sarah L Thomas Michelle Scurr Catherine Stanton Paul D Cotter Prue M Pereira-Fantini | 2015 | World Journal of Gastroenterology2015，21，14： | 3 |
| 2 | How to treat the Ewing's family of sarcomas in adult pati- ents显示文摘 | Scurr M Judson I | 2006 | Oneologist2006，11，1： | 1 |
| 3 | P161NK4a induced se- nescence is disabled by melanoma associated mutations 显示文摘 | Haferkamp S Beeker T M Scurr L L | 2008 | Aging Cell2008，7，5： | 1 |
| 4 | Phase I pharmacoki netic and pharmacodynamie study of 17 allylamino, 17-deme thoxygeldanamycin in patients with advanced malignancies显示文摘 | Banerji U O'Donnell A Scurr M | 2005 | Clin Oncol2005，23，18： | 1 |
| 5 | pl61NK4a induced se- nescence is disabled by melanoma associated mutations 显示文摘 | Haferkamp S Becker T M Scurr L L | 2008 | Aging cell2008，7，5： | 1 |
| 6 | T cell subsets and colorectalcancer:Discerning the good from the bad显示文摘 | Scurr M Gallimore A Godkin A | 2012 | Cell Immunol2012，279，1： | 1 |
| 7 | Pegylated liposomal doxorubicin,an effective,well-tolerated treatment for refractory aggressive fibromatosis显示文摘 | Constantinidou A Jones RL Scurr M | | 0，，17： | 1 |
| 8 | Advanced aggre-ssive fibromatosis:Effective palliation with chemotherapy显示文摘 | Constantinidou A Jones RL Scurr M | | 0，，03： | 1 |
| 9 | Pegylated liposomal doxo- rubicin an effective well-tolerated treatment for refraclory aggressive fi- bromatosis显示文摘 | Constantinidou A Jones RL Scurr M | 2009 | Eur J Cancer2009，45，17： | 1 |
| 10 | Predicting response of colorectal hepatic metastasis:value of pretreatment apparent diffusion coefficients显示文摘 | Koh D M Scurr E Collins D | 2007 | AJR2007，188，4： | 1 |
| 11 | pl4ARF regulates E2F-1 ubiquiti- nation and degradation via a p53-dependent mechanism 显示文摘 | Rizos H Scurr LL Irvine M | 2007 | Cell Cycle2007，6，14： | 1 |
| 12 | T cell subsets and colorectal cancer: discerning the good from the bad显示文摘 | Scurr M Gallimore A Godkin A | 2012 | Cell Immunol2012，279，1： | 1 |
| 13 | Coloreetal hepatic metasta- ses:quantitative measurements using single-shot echo-planar dif- fusion-weighted MR imaging显示文摘 | Koh D M Scurr E Collins D J | 2006 | Eur Radiol2006，16，9： | 1 |
| 14 | Magnetic reso- nance imaging in prostate cancer: the value of apparent diffusion coefficients for identifying malignant nodules 显示文摘 | Desouza N M Reinsberg S A Scurr E D | 2007 | Br J Radiol2007，80，950： | 1 |
| 15 | Highly prevalent colorectal cancer-infiltrating LAP+Foxp3-T cells exhibit morepotent immunosuppressive activity than Foxp3' regulatory T cells显示文摘 | Scurr M Ladell K Besneux M | 2014 | Mucosal Immunol2014，7，2： | 1 |
| 16 | Phase Ⅰ pharmacokinetie and pharmacodynamic study of 17 allylamino 17-demethoxy- geldanamycin in patients with advanced rnalignancies显示文摘 | Banerji U O'Donnell A Scurr M | 2005 | Journal of Clinical Oncology2005，23，18： | 1 |
| 17 | Phase I pharmacokinetic and pharmacodynamic studyof17-allylamino,17-demethoxygeldanamycin in patients with advanced malignancies显示文摘 | Banerji U O'Donnell A Scurr M | 2005 | J Clin Oncol2005，23，18： | 1 |
| 18 | Predicting response of colo?rectal hepatic metastasis: value of pretreatment apparent diffu?sion coefficients显示文摘 | Koh D M Scurr E Collins D | 2007 | AJR2007，188，4： | 1 |
| 19 | N eoadj uvant and adj uvant therapy for extremity soft tissue sarcomas显示文摘 | Scurr M Judson I | 2005 | Hematol Oncol Clin North Am2005，19，3： | 1 |
| 20 | Phase I /II study of DHA-paclitaxel in combination with carboplatin in patients with advanced malignant solid tumours显示文摘 | HARRIES M O'DONNELL A SCURR M | 2004 | Br J Cancer2004，91，9： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员