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| 1 | Influenza A Virus(H1N1) Infection Induces Glycolysis to Facilitate Viral Replication显示文摘Viruses depend on host cellular metabolism to provide the energy and biosynthetic building blocks required for their replication. In this study, we observed that influenza A virus(H1N1), a single-stranded, negative-sense RNA virus with an eight-segmented genome, enhanced glycolysis both in mouse lung tissues and in human lung epithelial(A549) cells. In detail, the expression of hexokinase 2(HK2), the first enzyme in glycolysis, was upregulated in H1N1-infected A549 cells,and the expression of pyruvate kinase M2(PKM2) and pyruvate dehydrogenase kinase 3(PDK3) was upregulated in H1N1-infected mouse lung tissues. Pharmacologically inhibiting the glycolytic pathway or targeting hypoxia-inducible factor 1(HIF-1), the central transcriptional factor critical for glycolysis, significantly reduced H1N1 replication, revealing a requirement for glycolysis during H1N1 infection. In addition, pharmacologically enhancing the glycolytic pathway further promoted H1N1 replication. Furthermore, the change of H1N1 replication upon glycolysis inhibition or enhancement was independent of interferon signaling. Taken together, these findings suggest that influenza A virus induces the glycolytic pathway and thus facilitates efficient viral replication. This study raises the possibility that metabolic inhibitors, such as those that target glycolysis, could be used to treat influenza A virus infection in the future. | Lehao Ren Wanju Zhang Jing Zhang Jiaxiang Zhang Huiying Zhang Yong Zhu Xiaoxiao Meng Zhigang Yi Ruilan Wang | 2021 | Virologica Sinica2021，36，6： | 3 |
| 2 | Repeated pulse intramuscular in- jection of pralidoxime chloride in severe acute organophosphorus pesticide poisoning显示文摘 | Xue Tang Ruilan Wang Hui Xie | 2013 | American Journal of Emergency Medicine2013，31，6： | 1 |
| 3 | Nitric Oxide Enhances Angiogenesis via the Synthesis of Vascular Endothelial Growth Factor and cGMP After Stroke in the Rat显示文摘 | RUILAN ZHANG LEI WANG LI ZHANG | 2003 | Circ Res2003，92，： | 1 |
| 4 | New Fault Tolerant Robotic Central Controller for Space Robot System Based on ARM Processor显示文摘 | Xingsheng Wang Bin Liang Ruilan Wu | 2008 | Industrial Technology2008，，： | 1 |
| 5 | Risk factors and a prediction model for sepsis: A multicenter retrospective study in China显示文摘Background:Sepsis is typically associated with poor outcomes.There are various risk factors and predictive mod-els for sepsis based on clinical indicators.However,these models are usually predictive of all critical patients.This study explored the risk factors for 28-day outcomes of patients with sepsis and developed a prognosis prediction model.Methods:This was a multicenter retrospective analysis of sepsis patients hospitalized in three intensive care units(ICUs)from September 1st 2015,to June 30th 2020.Demographic,clinical history,and laboratory test data were extracted from patient records.Investigators explored the risk factors affecting 28-day sepsis prognosis by univariate analysis.The effects of confounding factors were excluded by multivariate logistic regression analysis,and new joint predictive factors were calculated.A model predicting 28-day sepsis prognosis was constructed through data processing analysis.Results:A total of 545 patients with sepsis were included.The 28-day mortality rate was 32.3%.Risk factors including age,D-dimer,albumin,creatinine,and prothrombin time(PT)were predictive of death from sepsis.The goodness-of-fit value for this prediction model was 0.534,and the area under the receiver operating characteristic curve was 0.7207.Further analysis of the immune subgroups(n=140)revealed a significant decrease in CD3+,CD4+CD8-,and CD4+CD29+memory effector T lymphocytes and an increase in CD56+natural killer(NK)cells in the hypoalbuminemia group compared with the normal albumin group(65.5 vs.58.3,P=0.005;41.2 vs.32.4,P=0.005;21.8 vs.17.1,P=0.029;12.6 vs.17.6,P=0.004).Conclusions:Risk factors for 28-day sepsis mortality include age,D-dimer,creatinine,PT,and albumin.A decrease in albumin level may exacerbate immunosuppression in patients with sepsis.This study establishes a prediction model based on these indicators,which shows a good degree of calibration and differentiation.This model may provide good predictive value for clinical sepsis prognosis. | Ming Li Peijie Huang Weiwei Xu Zhigang Zhou Yun Xie Cheng Chen Yihan Jiang Guangqing Cui Qi Zhao Ruilan Wang | 2022 | Journal of Intensive Medicine2022，2，3： | 1 |
| 6 | Repeated pulse intramuscular injection of pralidoxime chloride in severe acute organophosphorus pesticide poisoning显示文摘 | Xue Tang Ruilan Wang Hui Xie Jiachang Hu Wenbiao Zhao | 2013 | American Journal of Emergency Medicine2013，，6： | 1 |
| 7 | 心脏手术后低氧血症显示文摘心脏手术后常常发生低氧血症,但常常不引起外科医牛的重视。有些低氧血疗是一过性,对机体不会造成严重影响。但有些低氧血症会明显延长心脏手术后ICU日及平均住院日,增加死亡率。常见原因有心源性、肺源性(包括原有肺的疚病或无肺的基础疾病)。本文就无肺的基础疾病的心脏手术后产生低氧血症的一些情况进行阐述。肺不张、急性肺损伤、心脏手术后隔肌麻痹、手术方式等都是引起心脏手术后低氧血症的常见原因。心脏手术后隔肌麻痹不易诊断。本文就此问题叙述了几种常用方法的利和弊。研究认为在手术早期给予恰当的呼吸末正压及肺活量充气策略可以改善氧合状态。 | WANG Ruilan FU Huaqun | 2006 | 世界急危重病医学杂志2006，3，1： | 0 |
| 8 | Sand rice,a promising future crop for desert and marginal lands in northern China显示文摘The pioneer Amaranthaceae species sand rice(Agriophyllum squarrosum)is an annual psammophyte that is widely distributed in the deserts and sand fields of northern China.The well-balanced nutritional values,long consumption history,and extreme stress tolerance of sand rice have fascinated scientists,prompting its development as a climate-resilient crop.Sand rice has been successfully introduced and cultivated on sandy and loess lands over the past decade,while large-scale artificial planting has been carried out in the Ulan Buh and Tengger deserts.However,the yield is far below the maximum potential,as estimated by the highest yield per plant ever found in the Tengger desert during our survey of wild populations.The current domestication of sand rice relies mainly on natural selection and mutagenesis breeding.A few elite lines with modified agronomic traits,such as compact architecture,high productivity,reduced trichomes,and short plant stature,have been developed from natural populations and a chemical mutagenesis library.Breeding new cultivars and broader cultivation of sand rice in deserts and marginal lands will stimulate economic growth and diversify the food supply,especially for the area west of the Hu Huanyong Line,thus contributing to environmental sustainability in northern China. | Pengshan Zhao Ruilan Ran Hong Sun Yujie Liu Xiaofeng Li Changbao Wang Xin Zhao Guoxiong Chen | 2023 | Grassland Research2023，2，4： | 0 |
| 9 | Efficacy and safety of awake prone positioning in the treatment of non-intubated spontaneously breathing patients with COVID-19-related acute respiratory failure:A systematic review and meta-analysis显示文摘Background Since the beginning of the coronavirus disease 2019(COVID-19)pandemic,prone positioning has been widely applied for non-intubated,spontaneously breathing patients.However,the efficacy and safety of prone positioning in non-intubated patients with COVID-19-related acute hypoxemic respiratory failure remain unclear.We aimed to systematically analyze the outcomes associated with awake prone positioning(APP).Methods We conducted a systematic literature search of PubMed/MEDLINE,Cochrane Library,Embase,and Web of Science from January 1,2020,to June 3,2022.This study included adult patients with acute respiratory failure caused by COVID-19.The Preferred Reporting Items for Systematic Reviews and Meta-Analyses(PRISMA)guidelines were followed,and the study quality was assessed using the Cochrane risk-of-bias tool.The primary outcome was the reported cumulative intubation risk across randomized controlled trials(RCTs),and the effect estimates were calculated as risk ratios(RRs;95%confidence interval[CI]).Results A total of 495 studies were identified,of which 10 fulfilled the selection criteria,and 2294 patients were included.In comparison to supine positioning,APP significantly reduced the need for intubation in the overall population(RR=0.84,95%CI:0.74–0.95).The two groups showed no significant differences in the incidence of adverse events(RR=1.16,95%CI:0.48–2.76).The meta-analysis revealed no difference in mortality between the groups(RR=0.93,95%CI:0.77–1.11).Conclusions APP was safe and reduced the need for intubation in patients with respiratory failure associated with COVID-19.However,it did not significantly reduce mortality in comparison to usual care without prone positioning. | Jingjing Wang Daonan Chen Puyu Deng Chenchen Zhang Xue Zhan Hui Lv Hui Xie Dechang Chen Ruilan Wang | 2023 | Journal of Intensive Medicine2023，3，4： | 0 |
| 10 | Point-of-care Ultrasound as a Useful Tool for Assessment of Undifferentiated Dyspnea in Rapid Response Team Consultation显示文摘Objective The purpose of this study is to evaluate the role of point-of-care ultrasound( POCUS) in assessm ent of patients with dyspnea in Rapid Response Team( RRT)consultation. 53 patients were included and their pre-POCUS, post-POCUS and final diagnoses were docum ented. Com pared with pre-POCUS diagnoses,the accuracy of postPOCUS diagnoses increased significantly from 37.7% up to 79.2%,P<0.01( final diagnoses as golden standard). When stratified by etiology, we can see that POCUS im proved the diagnostic sensitivity and specificity in m ost of the diseases. But still there were 11 patients in whom POCUS fail to diagnose. And in those POCUS-fail group the m ortality seem ed higher than in POCUS achieved group. In conclusion POCUS is a convenient and useful diagnostic tool which can im prove the diagnostic accuracy of patients with sudden onset of dyspnea in RRT consultation. | Du Jiang Lin Qiuhai Xie Hui Zhou Zhigang Wang Ruilan | 2018 | 中华肺部疾病杂志(电子版)2018，11，3： | 0 |
| 11 | HIF-1αpromotes virus replication and cytokine storm in H1N1 virus-induced severe pneumonia through cellular metabolic reprogramming显示文摘The mortality of patients with severe pneumonia caused by H1N1 infection is closely related to viral replication and cytokine storm.However,the specific mechanisms triggering virus replication and cytokine storm are still not fully elucidated.Here,we identified hypoxia inducible factor-1α(HIF-1α)as one of the major host molecules that facilitates H1N1 virus replication followed by cytokine storm in alveolar epithelial cells.Specifically,HIF-1αprotein expression is upregulated after H1N1 infection.Deficiency of HIF-1αattenuates pulmonary injury,viral replication and cytokine storm in vivo.In addition,viral replication and cytokine storm were inhibited after HIF-1αknockdown in vitro.Mechanistically,the invasion of H1N1 virus into alveolar epithelial cells leads to a shift in glucose metabolism to glycolysis,with rapid production of ATP and lactate.Inhibition of glycolysis significantly suppresses viral replication and inflammatory responses.Further analysis revealed that H1N1-induced HIF-1αcan promote the expression of hexokinase 2(HK2),the key enzyme of glycolysis,and then not only provide energy for the rapid replication of H1N1 virus but also produce lactate,which reduces the accumulation of the MAVS/RIG-I complex and inhibits IFN-α/βproduction.In conclusion,this study demonstrated that the upregulation of HIF-1αby H1N1 infection augments viral replication and cytokine storm by cellular metabolic reprogramming toward glycolysis mainly through upregulation of HK2,providing a theoretical basis for finding potential targets for the treatment of severe pneumonia caused by H1N1 infection. | Xiaoxiao Meng Yong Zhu Wenyu Yang Jiaxiang Zhang Wei Jin Rui Tian Zhengfeng Yang Ruilan Wang | 2024 | Virologica Sinica2024，39，1： | 0 |
| 12 | STUDY ON COPYING QUALITY IN CONTOUR GRINDING显示文摘STUDYONCOPYINGQUALITYINCONTOURGRINDINGSTUDYONCOPYINGQUALITYINCONTOURGRINDINGWangWanshan;WangBo;LuShubin;ShanRuilan(Northeaste... | Wang Wanshan Wang Bo Lu Shubin Shan Ruilan(Northeastern University)Guo Changsheng(University of Massachusetts, U .S.A.) | 1995 | Chinese Journal of Mechanical Engineering1995，8，1： | 0 |
| 13 | Correlation between clinical course and radiographic development on CT scan in patients with COVID-19显示文摘Background:To analyze the correlation between clinical course and radiographic development on computed tomography(CT)in patients with confirmed coronavirus disease 2019(COVID-19)and to provide more evidence for treatment.Methods:This retrospective,observational,cohort study enrolled 49 patients with Reverse transcription-polymerase chain reaction(RT-PCR)-confirmed COVID-19,which included 30 patients admitted to the intensive care unit(ICU)of Wuhan Third Hospital and 19 patients either admitted to or receiving telemedicine consultation from Shanghai General Hospital,Shanghai Xuhui Dahua Hospital,and hospitals in other provinces.CT scans were performed in all enrolled patients and the radiographic features including simple ground-glass opacities(GGOs),GGO with interlobular septal thickening,consolidations with GGO,and consolidations only were monitored by repeating the CT.The progression of these radiographic features was analyzed in combination with their clinical staging and the time interval between onset of symptoms to CT.Results:Based on illness severity,the 49 patients were classified into four stages:mild(n=6),moderate(n=12),severe(n=16),and critically ill(n=15).The CT findings were classified into three phases:early(n=5),progres-sion(n=39),and recovery(n=5).Among the 49 patients,9 had bilateral diffuse GGO or diffuse consolidations(white lungs)and were counted as 18 lesions.Three patients had no abnormal findings on initial CT,but their repeat CT showed new lesions.In all,we identified 892 lesions including simple GGO,GGO with interlobular septal thickening,consolidations with GGO,and consolidations only.Conclusions:Most patients had pulmonary lesions on the posterior,inferior,and peripheral lung fields on CT.The development of GGO with interlobular septal thickening,GGO with consolidations,and consolidations only happened mainly between day 8 and 14.The emergence of consolidations may suggest the progression to the severe phase of the illness,whereas simple consolidations or“white lung”may suggest a critically ill phase. | Yun Xie Luyu Yang Hui Dong Song Cao Wenkai Zhang Qiong Chen Baoliang Jin Zhiguo Zhou Jun Liu Zhiyan He Ruilan Wang | 2021 | Journal of Intensive Medicine2021，1，1： | 0 |
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