|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Precise in vivo genome editing via single homology arm donor mediated intron-targeting gene integration for genetic disease correction显示文摘In vivo genome editing represents a powerful strategy for both understanding basic biology and treating inherited diseases.However,it remains a challenge to develop universal and efficient in vivo genome-editing tools for tissues that comprise diverse cell types in either a dividing or non-dividing state.Here,we describe a versatile in vivo gene knock-in methodology that enables the targeting of a broad range of mutations and cell types through the insertion of a minigene at an intron of the target gene locus using an intracellularly linearized single homology arm donor.As a proof-of-concept,we focused on a mouse model of prematureaging caused by a dominant point mutation,which is difficult to repair using existing in vivo genome-editing tools.Systemic treatment using our new method ameliorated aging-associated phenotypes and extended animal lifespan,thus highlighting the potential of this methodology for a broad range of in vivo genome-editing applications. | Keiichiro Suzuki Mako Yamamoto Reyna Hernandez-Benitez Zhe Li Christopher Wei Rupa Devi Soligalla Emi Aizawa Fumiyuki Hatanaka Masakazu Kurita Pradeep Reddy Alejandro Ocampo Tomoaki Hishida Masahiro Sakurai Amy NNemeth Estrella Nunez Delicado Josep MCampistol Pierre Magistretti Pedro Guillen Concepcion Rodriguez Esteban Jianhui Gong Yilin Yuan Ying Gu Guang-Hui Liu Carlos Lopez-Otin Jun Wu Kun Zhang Juan Carlos Izpisua Belmonte | 2019 | Cell Research2019，29，10： | 2 |
| 2 | Inhibition of Listeria monocytogenes by Enterocin 4 During the Manufacture and Ripening of Manchego Cheese显示文摘 | Nunez M Rodriguez J L Garcia E | 1997 | J Appl Microbiol1997，83，6： | 1 |
| 3 | Inhibition of Listeria monocytogenes by enterocin 4 during the manufac- ture and ripening of Manchego cheese显示文摘 | Nunez M Rodriguez JL Garcia E | 1997 | Journal of Applied Bacteriology1997，83，6： | 1 |
| 4 | Composition and toxigenic potential of the mould population on drycured Iberian ham显示文摘 | Nunez F Rodriguez M M Bermudez M E | 1996 | International Journal of Food Microbiology1996，32，： | 1 |
| 5 | Composition and toxig-enic potential of the mould population on dry-cured Iberian ham显示文摘 | Nunez F Rodriguez M M Bermudez M E | 1996 | International Journal of Food Microbiology1996，32，： | 1 |
| 6 | Abdominal pelvic actinomycosis with urinary tract involvenment,secondary to gynecologic infection caused by intrauterine divices显示文摘 | Perez Garcia MD Rodriguez Alonso A Nunez Lopez A | 2000 | Acfas Urol Esp2000，24，2： | 1 |
| 7 | Effects of substrate,water activity,and tempcrature on growth and verrucosidin production by Penicillium polonicum isolated from dry-cured ham显示文摘 | Nunez F Diaz M C Rodriguez M | 2000 | Journal of Food Protection2000，63，： | 1 |
| 8 | Peptide generation in the processing of dry - cured ham 显示文摘 | Eugenio Rodriguez Nunez Maria - Conception Aristoy and Fidel Toldra | 1995 | Food Chemistry1995，，53： | 1 |
| 9 | Ethanol increases tumor necrosis factor-alpha receptor-1(TNF-R1)levels in hepatic,intestinal,and cardiac cells显示文摘 | Rodriguez DA Moncada C Nunez MT | 2004 | Alcohol2004，33，1： | 1 |
| 10 | Cytosolic levels of neuron-specific enolase in squamous cell carcinomas of the lung显示文摘 | RUIBALA NUNEZ M I RODRIGUEZ J | 2003 | Biol Markers2003，18，： | 1 |
| 11 | Evaluation of proteolytic activity of micro-organisms isolated from dry cured ham 显示文摘 | Rodriguez MM Nunez F Cordoba JJ | 1998 | Journal of Applied Microbiology1998，，85： | 1 |
| 12 | Biochemical parameters predictive of neuronal damage in childrood 显示文摘 | Costro Gago M Rodriguez Nunez A Noro Rodriguez MI | 2001 | Rev Nearot2001，32，3： | 1 |
| 13 | An ticonvulsant effect of eslicarbazepine acetate ( BIA 2 - 093 ) on seizures induced by microperfusion of picrotoxin in the hippocampus of freely moving rats 显示文摘 | Sierra - Paredes G Nunez - Rodriguez A Vazquez - Lopez A | 2006 | Epilepsy Res2006，72，23： | 1 |
| 14 | Cytosolic levels of neuron-specific enolase in squamous cell carcinomas of the lung显示文摘 | Ruibal A Nunez MI Rodriguez J | 2003 | Int J Biol Marker2003，18，3： | 1 |
| 15 | Ethanol increases tumor necrosis factor-alpha receptor-1(TNF-R1)levels in hepatic intestinal,and cardiac cells显示文摘 | Rodriguez DA Moncada C Nunez MT | 2004 | Alcohol2004，33，1： | 1 |
| 16 | Plasma vitellogenin levels during the annual reproductive cyle of the female rainbow trout (Oncorhynchus mykiss): establishment and validation of an ELISA 显示文摘 | Bon E Barbe U Nunez Rodriguez J | 1997 | Comparative Biochemistry and Physiology1997，117，： | 1 |
| 17 | Effects of substrate, water activity, and temperature on growth and verrucosidin production by Penicillium polonicum isolated from dry-cured ham 显示文摘 | Nunez F Diaz M C Rodriguez M | 2000 | Journal of Food Protection2000，，63： | 1 |
| 18 | Nuclear and chloroplast DNA reassessment of the origin of Indian potato varieties and its implications for the origin of the early European potato显示文摘 | SPOONER D M NUNEZ J RODRIGUEZ F NAIK P S GHISLAIN M | 2005 | Theoretical and Applied Genetics2005，110，： | 1 |
| 19 | Burnout syndrome among health workers in pediatrics显示文摘 | Lopez Franco M Rodriguez Nunez A Fernandez Sanmartin M | 2005 | An pediatr(Barc)2005，62，3： | 1 |
| 20 | Peptide gen- eration in the processing of dry-cured ham显示文摘 | RODRIGUEZ NUNEZ E ARISTOY M C TOLDRA F | 1995 | Food Chemistry1995，53，2： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员