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| 1 | Osteopontin increases hepatocellular carcinoma cell growth in a CD44 dependant manner显示文摘AIM:To investigate the role of osteopontin(OPN) and its splice variants in the proliferation of hepatocellular carcinoma(HCC).METHODS:The expression of OPN variants in HCC cell lines as well as HCC tissue samples and nontumour tissue was studied using polymerase chain reaction.OPN variant cDNAs were cloned into a mammalian expression vector allowing both transient expression and the production of stable OPN expressing cell lines.OPN expression was studied in these cells using Western blotting,immunofluoresnce and enzyme linked immunosorbent assay.A CD44 blocking antibody and siRNA targeting of CD44 were used to examine the role of this receptor in the OPN stimulated cell growth observed in culture.Huh-7 cells stably expressing either OPN-A,-B or-C were injected subcutaneously into the flanks of nude mice to observe in vivo tumour growth.Expression of OPN mRNA and protein in these tumours was examined using reverse transcriptionpolymerase chain reaction and immunohistochemistry.RESULTS:OPN is expressed in HCC in 3 forms,the full length OPN-A and 2 splice variants OPN-B and-C.OPN variant expression was noted in HCC tissue as well as cognate surrounding cirrhotic liver tissue.Expression of these OPN variants in the HCC derived cell line Huh-7 resulted in secretion of OPN into the culture medium.Transfer of OPN conditioned media to na ve Huh-7 and HepG2 cells resulted in significant cell growth suggesting that all OPN variants can modulate cell proliferation in a paracrine manner.Furthermore the OPN mediated increase in cellular proliferation was dependent on CD44 as only CD44 positive cell lines responded to OPN conditioned media while siRNA knockdown of CD44 blocked the proliferative effect.OPN expression also increased the proliferation of Huh-7 cells in a subcutaneous nude mouse tumour model,with Huh-7 cells expressing OPN-A showing the greatest proliferative effect.CONCLUSION:This study demonstrates that OPN plays a significant role in the proliferation of HCC through interaction with the cell surface receptor CD44.Modulation of this interaction could represent a novel strategy for the control of HCC. | Renee J Phillips Karla J Helbig Kylie H Van der Hoek Devanshi Seth Michael R Beard | 2012 | World Journal of Gastroenterology2012，18，26： | 12 |
| 2 | Colonoscopic yield of colorectal neoplasia in daily clinical practice显示文摘AIM:To assess the prevalence and location of ad-vanced neoplasia in patients undergoing colonoscopy,and to compare the yield per indication.METHODS:In a multicenter colonoscopy survey (n = 18 hospitals) in the Amsterdam area (Northern Holland),data of all colonoscopies performed during a three month period in 2005 were analyzed. The location and the histological features of all colonic neoplasia were recorded. The prevalence and the distribution ofadvanced colorectal neoplasia and differences in yield between indication clusters were evaluated. Advanced neoplasm was defi ned as adenoma > 10 mm in size,with > 25% villous features or with high-grade dyspla-sia or cancer.RESULTS:A total of 4623 eligible patients underwent a total colonoscopy. The prevalence of advanced neo-plasia was 13%,with 281 (6%) adenocarcinomas and 342 (7%) advanced adenomas. Sixty-seven percent and 33% of advanced neoplasia were located in the distal and proximal colon,respectively. Of all patients with right-sided advanced neoplasia (n = 228),51% had a normal distal colon,whereas 27% had a syn-chronous distal adenoma. Ten percent of all colono-scopies were performed in asymptomatic patients,7% of whom had advanced neoplasia. In the respective procedure indication clusters,the prevalence of right-sided advanced neoplasia ranged from 11%-57%. CONCLUSION:One out of every 7-8 colonoscopies yielded an advanced colorectal neoplasm. Colonoscopy is warranted for the evaluation of both symptomatic and asymptomatic patients. | Jochim S Terhaar sive Droste Mike E Craanen Rene WM van der Hulst Joep F Bartelsman Dick P Bezemer Kim R Cappendijk Gerrit A Meijer Linde M Morsink Pleun Snel Hans ARE Tuynman Roy LJ van Wanrooy Eric IC Wesdorp Chris JJ Mulder | 2009 | World Journal of Gastroenterology2009，15，9： | 6 |
| 3 | Gastrointestinal disorders associated with migraine: A comprehensive review显示文摘Migraine is a recurrent and commonly disabling primary headache disorder that affects over 17% of women and 5%-8% of men. Migraine susceptibility is multifactorial with genetic, hormonal and environmental factors all playing an important role. The physiopathology of migraine is complex and still not fully understood. Many different neuropeptides, neurotransmitters and brain pathways have been implicated. In connection with the myriad mechanisms and pathways implicated in migraine, a variety of multisystemic comorbidities(e.g., cardiovascular, psychiatric and other neurological conditions) have been found to be closely associated with migraine. Recent reports demonstrate an increased frequency of gastrointestinal(GI) disorders in patients with migraine compared with the general population. Helicobacter pylori infection, irritable bowel syndrome, gastroparesis, hepatobiliary disorders, celiac disease and alterations in the microbiota have been linked to the occurrence of migraine. Several mechanisms involving the gut-brain axis, such as a chronic inflammatory response with inflammatory and vasoactive mediators passing to the circulatory system, intestinal microbiota modulation of the enteric immunological milieu and dysfunction of the autonomic and enteric nervous system, have been postulated to explain these associations. However, the precise mechanisms and pathways related to the gut-brain axis in migraine need to be fully elucidated. In this review, we survey the available literature linking migraine with GI disorders. We discuss the possible physiopathological mechanisms, and clinical implications as well as several future areas of interest for research. | Carlos R Cámara-Lemarroy Rene Rodriguez-Gutierrez Roberto Monreal-Robles Alejandro Marfil-Rivera | 2016 | World Journal of Gastroenterology2016，22，36： | 6 |
| 4 | Methods for the development and assessment of atrial fibrillation and heart failure dog models显示文摘与动物的发展报导 Medtronic 经验的目的用为 AF 模型,的为 CHF.Methods 的为 AF 和 microemboli 的高率的踱步为 atrial 纤维性颤动( AF )和长期的心失败( CHF )当模特儿一根 atrial 皮带被纳入 Medtronic 协同作用 neurostimulator ,它被规划在开关责任 cycle.Atrial natnuretic 肽( ANP )在 50 Hz 刺激,大脑 natriuretic 肽( BNP )和N终端职业人员大脑 natriuretic 肽( NT-proBNP )皮脂腺囊瘤;在精选时间 points.For CHF 模型,的 assayed 在 62,400 beads/mL 的 90 m 聚苯乙烯 microspheres 的连续注射( Polybead , Polysciences 公司)被执行导致全球局部缺血,有每周监视的任何一个和 embolization 时间表(集团 1 , n = 25 )或与监视的双周刊和 emboliation 时间表(集团 2 , n36 ) .Echocardiograms 每周与 ventriculograms 和磁性的回声成像扫描一起被使用估计心脏的功能和 ANP , BNP 和 NT-proBNP 是为 AF 模型的 monitored.Results ,为 CHF 模型,遇见 CHF 端点的日子在集团 2 .For 在集团 1 和 89 是 116 AF 和 CHF 模型, NT-proBNP 与在为动物的搜索的研究人员为估计基于设备的治疗的安全和功效建模的我们为 AF 和 CHF 狗模型的发展和评价的经验可以帮助的疾病 states.Conclusion 的发展相关很好。 | Jon F Urban Renee L Gerhart Jason R Krzeszak Corey R Leet Linnea R Lentz Carolyn B McClay | 2011 | Journal of Geriatric Cardiology2011，8，3： | 3 |
| 5 | The DA VINCI Study: Phase 2 Primary Results of VEGF Trap-Eye in Patients with Diabetic Macular Edema显示文摘 | Diana V. Do Ursula Schmidt-Erfurth Victor H. Gonzalez Carmelina M. Gordon Michael Tolentino Alyson J. Berliner Robert Vitti Rene Rückert Rupert Sandbrink David Stein Ke Yang Karola Beckmann Jeff S. Heier | 2011 | Ophthalmology2011，，9： | 3 |
| 6 | Propranolol for the prevention of postoperative arrhythmias in general thoracic surgery显示文摘 | Charles D Bayliff David R Massel Richard I Inculet Richard A Malthaner Susan D Quinton Frank S Powell Renee S Kennedy | 1999 | The Annals of Thoracic Surgery1999，，1： | 2 |
| 7 | Anti-Hu antibodies in patients with small-cell lung cancer:association with complete response to therapy and improved survival显示文摘 | Dalmou J Rene R | 1997 | J Clin Oncol1997，15，8： | 1 |
| 8 | Two-dimensional current percolation in nanocrystalline vana- dium dioxide films 显示文摘 | Rozen John Lopez Rene Haglund R F | 2006 | Applied Physics Letters2006，88，08： | 1 |
| 9 | Plant-soil relationships of the invnsive annual grass TaenloJhemm caput-medusae:a reciprocal transplant experiment显示文摘 | Blank R B Rene'Sforza | 2007 | Plant Soil2007，298，12： | 1 |
| 10 | Frontal and associative visual areas related to visual hallucinations in dementia with Lewy bodies and Parkinson′s disease with dementia显示文摘 | Sanchez-Castaneda C Rene R Ramirez-Ruiz B | | 0，，： | 1 |
| 11 | Randomised comparison of combined step-down prednisolone, methotrexate and sulphasalazine with sulphasalazine alone in early rheumatoid arthritis显示文摘 | Maarten Boers Arco C Verhoeven Harry M Markusse Mart AFJ van de Laar Rene Westhovens J Christiaan van Denderen Derkjen van Zeben Ben AC Dijkmans Andre J Peeters Piet Jacobs Hans R van den Brink Hubert JA Schouten Désirée MFM van der Heijde Annelies Boonen | 1997 | The Lancet1997，，9074： | 1 |
| 12 | ( + )-And (-)-borneol: efficacious positive modulators of GABAaction at human recombinant ell,82 2L GABAA receptors 显示文摘 | Renee E Granger Erica L Campbell Graham A R Johnston | 2005 | Bio- chem Pharmaco12005，69，： | 1 |
| 13 | Measuring the environmental load of household consumption using some methods based on input output energy analysis: A comparison of methods and a discussion of results 显示文摘 | Kok R Rene M J B Henri C M | 2006 | Energy Policy2006，34，17： | 1 |
| 14 | Short bowel syndrome and crohn’s disease显示文摘 | Jon S Thompson Kishore R Iyer John K DiBaise Renee L Young Cindy R Brown Alan N Langnas | 2003 | Journal of Gastrointestinal Surgery2003，，8： | 1 |
| 15 | Ethylene polymerization using dealuminated ZSM-2 zeolite nanocrystals as an active metallocene catalyst support显示文摘 | Cristian C Raul Q Rene R | 2008 | Applied catalysis A: General2008，347，2： | 1 |
| 16 | Contracting processes and structures for systems of-systems acquisition显示文摘 | Rene G R Thomas V H John S O | 2012 | Systems Engi- neering2012，15，4： | 1 |
| 17 | Detection of mam- maglobin mRNA in peripheral blood is associated with high grade breast cancer: Interim results of a prospective cohort study显示文摘 | Kaidi M Renee H M Megan B R | 2008 | BMC Cancer2008，8，： | 1 |
| 18 | Cooperative effects in differentiation and proliferation between PDGF-BB and matrix derived synthetic peptides in human osteoblasts显示文摘 | Vordemvenne Thomas Paletta Juergen R J Hartensuer Rene | | 0，，12： | 1 |
| 19 | One-stage biotrickling filter for the removal of a mixture of volatile pollutants from air:Performance and microbial community analysis显示文摘 | López M E Eldon Rene E R Malhautier L | 2013 | Bioresour Technol2013，138，： | 1 |
| 20 | Design and control of warehouse order picking: A literature review显示文摘 | RENE D K LE-DUC T KEES J R | 2007 | European Journal of OperationaIReseareh2007，182，2： | 1 |
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