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| 1 | 一个新的水稻逆境响应基因OsMsr1的表达与克隆显示文摘为深入了解植物的逆境反应机理和发现新的水稻耐逆基因,采用Affymetrix水稻表达芯片(含51279个转录本)分析了超级稻两优培九母本培矮64S不同生长发育时期、不同组织器官全基因组在低温、干旱、高温逆境胁迫下的表达水平,筛选出众多表达水平显著升高与降低的基因。OsMsr1(Oryza sativa L.multiple stresses responsive gene 1,Gen Bank登录号为EU284112)是其中一个受高温、低温与干旱诱导、在各生长发育时期与组织器官均显著上调的基因,用实时定量PCR方法对其表达水平进行了进一步的分析,所得结果与基因芯片结果基本吻合,因此,此基因为一多逆境响应基因。用RT-PCR方法扩增获得了包含其完整ORF(open reading frame)的cDNA克隆。根据其ORF序列进行预测,此基因编码一个包含89个氨基酸残基的小分子蛋白,分子量约为10kD,pI约为5。搜索有关数据库,在水稻、玉米、小麦与拟南芥中找到有高相似性的基因,但功能未知,也未发现相同与类似的已知功能的基因保守结构域。对其可能的启动子序列分析,发现5个与逆境反应有关的顺式作用元件。因此,我们认为该基因为一新的水稻耐逆候选基因,进一步的研究正在进行。 | 徐孟亮 陈荣军 ROCHA Pedro 李落叶 王曼玲 徐国云 夏新界 | 2008 | 作物学报2008，34，10： | 17 |
| 2 | 一个新的水稻低温应答类糖基转移酶基因(OsCrGtl)的表达分析与克隆显示文摘采用Affymetrix60K水稻基因表达芯片系统分析了水稻培矮64S(Oryza sativa ssp.indica)在低温、高温和干旱条件下不同生育时期的叶片与穗全基因组的转录丰度,筛选出一个类糖基转移酶基因OsCrGtl(Oryza sativ a cold responsive glycosyltransferase-like gene,GenBank登录号为FJ828672),该基因主要受低温诱导且在各生育时期的表达量均显著上调。用实时定量RT-PCR方法对该基因的在逆境条件下的转录丰度进行了验证分析,也得到与基因芯片分析基本相同的结果。以培矮64SmRNA为模板,通过RT-PCR方法扩增,得到包含其完整开放阅读框的cDNA克隆。根据其开放阅读框的核苷酸序列推测,OsCrGtl编码一个包含462个氨基酸残基的蛋白质,理论分子量为50.327kD,等电点为5.35。查询蛋白质数据库,得知该基因编码的蛋白质与籼稻(indica)、粳稻(japonica)、玉米(Zea mays)及拟南芥(Arabidopsis thaliana)编码的糖基转移酶家族蛋白相似,且包含糖基转移酶结构域。对其推测的启动子区域核苷酸序列进行分析,找到9个可能与逆境应答有关的顺式作用元件。 | 徐孟亮 李落叶 陈荣军 Rocha Pedro 张俊 王曼玲 徐国云 夏新界 | 2010 | 农业生物技术学报2010，18，4： | 10 |
| 3 | 应用基因表达芯片分析水稻高温胁迫相关基因显示文摘非生物逆境,如低温、高温、干旱通常会严重影响到农作物的生长及产量,其中高温胁迫则是造成植物伤害的主要原因之一。为深入了解水稻高温胁迫反应的分子机理,发现新的耐高温相关功能基因,为水稻生物工程育种提供候选材料,采用Affymetrix水稻表达芯片分析了超级稻两优培九母本培矮64S(Oryzasativa L.)在高温逆境胁迫下,孕穗期、抽穗开花期的叶片全基因组表达谱,得到大量高温诱导表达基因。应用实时定量PCR方法对其中一部分基因的表达水平进一步分析,所得结果与基因芯片结果基本吻合,证明芯片分析数据是可靠的,为下一步耐高温相关基因的克隆、功能分析等研究提供了基础。 | 王曼玲 Rocha Pedro 李落叶 徐孟亮 Din Chen 夏新界 | 2009 | 生物技术通报2009，25，10： | 5 |
| 4 | 水稻多逆境诱导基因OsMsr4的克隆与表达分析显示文摘为深入了解水稻逆境反应的分子机理和发现新的耐逆相关功能基因,采用A ffym etrix水稻表达芯片分析超级稻两优培九母本培矮64S(O ryza sativaL.)不同生长发育时期、不同组织器官全基因组在低温、干旱、高温逆境胁迫下的表达水平,筛选出多个多因子诱导高表达特异基因(待另文发表)。O sM sr4是其中一个在多种逆境条件,各生长发育时期与组织器官,其表达量均显著上调的基因,用实时定量PCR方法对其表达水平进行了进一步的分析,所得结果与基因芯片结果基本吻合。用PCR方法扩增获得长为550 bp全长基因序列,其编码的144个氨基酸残基形成Cys2H is2型双锌指结构蛋白,并且锌指结构的α-螺旋区含有植物锌指蛋白特定的保守序列QALGGH。因此,OsM sr4有可能是TFⅢA型锌指蛋白,作为转录因子参与各种环境胁迫应答反应,调控多个逆境相关基因表达。 | 王曼玲 Rocha Pedro 李落叶 徐孟亮 夏新界 | 2009 | 生物技术通报2009，25，7： | 3 |
| 5 | Gemcitabine Doublets in Advanced Pancreatic Cancer: Should We Move On? 显示文摘 | Caio Max Sao Pedro Rocha Lima and Aurea Maria Flores | 2006 | J Clin Oncol2006，24，3： | 1 |
| 6 | Shear effects on hollow section piers under seismic actions: experimental and numerical analysis显示文摘 | Raimundo Delgado Pedro Delgado Nelson Vila Pouca António Arêde Patrício Rocha Aníbal Costa | 2009 | Bulletin of Earthquake Engineering2009，，2： | 1 |
| 7 | Prevalence and Pathogenesis of Duodenal Ulcer in Chronic Alcoholic Pancreatitis显示文摘 | Julio Maria Fonseca Chebli Aécio Flávio Meirelles de Souza Pedro Duarte Gaburri Kátia Valeria Bastos Tarsila Campanha Rocha Ribeiro Roberto José Carvalho Filho Liliana Andrade Chebli Lincoln Eduardo V.V. Castro Ferreira | 2002 | Journal of Clinical Gastroenterology2002，，1： | 1 |
| 8 | Gemcitabine Doublets in Advanced Pancreatic Cancer: Should We Move On显示文摘 | Caio Max Sao Pedro Rocha Lima Aurea Maria Florcs | 2006 | Journal of Clinical Oncology2006，24，3： | 1 |
| 9 | Elevated nitric oxide and 3',5' cyclic guanosine monophosphate levels in patients with alcoholic cirrhosis显示文摘AIM: To evaluate whether serum levels of nitric oxide (NO ·) and plasma levels of cyclic guanosine monophosphate (cGMP) and total glutathione (GSH) are altered in patients with alcoholic cirrhosis and to examine their correlation with the severity of liver disease.METHODS: Twenty-six patients with alcoholic liver cirrhosis were studied. Serum levels of NO · and plasma levels of cGMP and GSH were measured in 7 patients with compensated alcoholic cirrhosis (Child-Pugh A) and 19 patients with advanced cirrhosis (Child-Pugh B and C). The model for end-stage liver disease (MELD) score was evaluated. Sixteen healthy volunteers served as controls. Liver enzymes and creatinine levels were also tested.RESULTS: NO · and cGMP levels were higher in patients with Child-Pugh B and C cirrhosis than in Child-Pugh A cirrhosis or controls (NO ·: 21.70 ± 8.07 vs 11.70 ± 2.74; 21.70 ± 8.07 vs 7.26 ± 2.47 μmol/L, respectively; P < 0.001) and (cGMP: 20.12 ± 6.62 vs 10.14 ± 2.78; 20.12 ± 6.62 vs 4.95 ± 1.21 pmol/L, respectively; P < 0.001). Total glutathione levels were lower in patients with Child-Pugh B and C cirrhosis than in patients with Child-Pugh A cirrhosis or controls (16.04 ± 6.06 vs 23.01 ± 4.38 or 16.04 ± 6.06 vs 66.57 ± 26.23 μmol/L, respectively; P < 0.001). There was a significant correlation between NO · and cGMP levels in all patients with alcoholic cirrhosis. A significant negative correlation between reduced glutathione/glutathione disulfide and the MELD score was found in all cirrhotic patients.CONCLUSION: Our results suggest a role for oxidative stress in alcoholic liver cirrhosis, which is more significant in decompensated patients with higher levels of NO ·and cGMP and lower GSH levels than in compensated and control patients. Altered mediator levels in decompensated patients may influence the hemodynamic changes in and progression of liver disease. | Cíntia Siqueira Miguel Carneiro de Moura Ana Júlia Pedro Paula Rocha | 2008 | World Journal of Gastroenterology2008，14，2： | 1 |
| 10 | Tailored welded blanks––an experimental and numerical study in sheet metal forming on the effect of welding显示文摘 | Ana Reis Pedro Teixeira J. Ferreira Duarte Abel Santos A. Barata da Rocha A.A. Fernandes | 2004 | Computers and Structures2004，，17： | 1 |
| 11 | A review of visible-range Fabry-Perot microspectrometers in silicon for the industry显示文摘 | Joo Paulo Carmo Rui Pedro Rocha Marian Bartek | 2012 | Optics and Laser Technology2012，，7： | 1 |
| 12 | Functional complementation of dwf4 mutants of Arabidopsis by overexpression of CYP724A1显示文摘 | Rujia Zhang Xinjie Xia Keith Lindsey Pedro S.C. Ferreira da Rocha | 2011 | Journal of Plant Physiology2011，，4： | 1 |
| 13 | Usefulness of serum C-reactive protein and calprotectin for the early detection of colorectal anastomotic leakage:A prospective observational study显示文摘BACKGROUND Colorectal anastomotic leakage(CAL)is one of the most dreaded complications after colorectal surgery,with an incidence that can be as high as 27%.This event is associated with increased morbidity and mortality;therefore,its early diagnosis is crucial to reduce clinical consequences and costs.Some biomarkers have been suggested as laboratory tools for the diagnosis of CAL.AIM To assess the usefulness of plasma C-reactive protein(CRP)and calprotectin(CLP)as early predictors of CAL.METHODS A prospective monocentric observational study was conducted including patients who underwent colorectal resection with anastomosis,from March 2017 to August 2019.Patients were divided into three groups:G1–no complications;G2–complications not related to CAL;and G3–CAL.Five biomarkers were measured and analyzed in the first 5 postoperative days(PODs),namely white blood cell(WBC)count,eosinophil cell count(ECC),CRP,CLP,and procalcitonin(PCT).Clinical criteria,such as abdominal pain and clinical condition,were also assessed.The correlation between biomarkers and CAL was evaluated.Receiver operating characteristic(ROC)curve analysis was used to compare the accuracy of these biomarkers as predictors of CAL,and the area under the ROC curve(AUROC),specificity,sensitivity,positive predictive value,and negative predictive value(NPV)during this period were estimated.RESULTS In total,25 of 396 patients developed CAL(6.3%),and the mean time for this diagnosis was 9.0±6.8 d.Some operative characteristics,such as surgical approach,blood loss,intraoperative complications,and duration of the procedure,were notably related to the development of CAL.The length of hospital stay was markedly higher in the group that developed CAL compared with the group with complications other than CAL and the group with no complications(median of 21 d vs 13 d and 7 d respectively;P<0.001).For abdominal pain,the best predictive performance was on POD4 and POD5,with the largest AUROC of 0.84 on POD4.Worsening of the clinical condition was associated with the diagnosis of CAL,presenting a higher predictive effect on POD5,with an AUROC of 0.9.WBC and ECC showed better predictive effects on POD5(AUROC=0.62 and 0.7,respectively).Those markers also presented a high NPV(94%-98%).PCT had the best predictive effect on POD5(AUROC=0.61),although it presented low accuracy.However,this biomarker revealed a high NPV on POD3,POD4,and POD5(96%,95%,and 96%,respectively).The mean CRP value on POD5 was significantly higher in the group that developed CAL compared with the group without complications(195.5±139.9 mg/L vs 59.5±43.4 mg/L;P<0.00001).On POD5,CRP had a NPV of 98%.The mean CLP value on POD3 was significantly higher in G3 compared with G1(5.26±3.58μg/mL vs 11.52±6.81μg/mL;P<0.00005).On POD3,the combination of CLP and CRP values showed a high diagnostic accuracy(AUROC=0.82),providing a 5.2 d reduction in the time to CAL diagnosis.CONCLUSION CRP and CLP are moderate predictors of CAL.However,the combination of these biomarkers presents an increased diagnostic accuracy,potentially decreasing the time to CAL diagnosis. | Nuno J G Rama Marlene C C Lages Maria Pedro S Guarino Óscar Lourenço Patrícia C Motta Lima Diana Parente Cândida S G Silva Ricardo Castro Ana Bento Anabela Rocha Fernando Castro-Pocas João Pimente | 2022 | World Journal of Gastroenterology2022，28，24： | 1 |
| 14 | Prevalence and Pathogenesis of Duodenal Ulcer in Chronic Alcoholic Pancreatitis显示文摘 | Julio Maria Fonseca Chebli Aécio Flávio Meirelles de Souza Pedro Duarte Gaburri Kátia Valeria Bastos Tarsila Campanha Rocha Ribeiro Roberto José Carvalho Filho Liliana Andrade Chebli Lincoln Eduardo V.V. Castro Ferreira | 2002 | Journal of Clinical Gastroenterology2002，，1： | 1 |
| 15 | Gemcitabine Doublets in Advanced Pancreatic Cancer:Should We Move On显示文摘 | Caio Max Sao Pedro Rocha Lima Aurea Maria Fiores | 2006 | J Clin Oncol2006，24，： | 1 |
| 16 | 水稻高温、干旱响应基因OsHdr5的克隆与表达谱分析显示文摘低温、高温、干旱等非生物逆境通常会严重影响到水稻的生长及产量.为深入了解水稻逆境反应的分子机理和挖掘耐逆相关基因,本文采用Affymetrix水稻表达芯片对水稻多逆境下的全基因组表达谱进行分析,并筛选出众多表达特异基因.OsHdr5是其中一个在多个生长发育时期与组织器官中受高温、干旱诱导均显著上调的基因,用实时定量PCR方法(real-time PCR)对其表达水平进一步分析,两组结果基本吻合.用PCR方法扩增获得长为743bp的全长基因序列,其ORF区编码167个氨基酸残基,组成的肽链含有磷酸化激酶位点,形成的二级结构包含一个由6个α螺旋和2个β折叠组成的跨膜结构域,进一步分析推测其可能是叶绿体膜上与细胞跨膜信号传递相关的功能蛋白. | 王曼玲 ROCHA Pedro 李落叶 徐孟亮 夏新界 | 2010 | 武汉大学学报(理学版)2010，56，1： | 0 |
| 17 | Remodeling of the cell wall as a drought-tolerance mechanism of a soybean genotype revealed by global gene expression analysis显示文摘Drought stress is major abiotic stress that affects soybean production.Therefore,it is widely desirable that soybean becomes more tolerant to stress.To provide insights into regulatory mechanisms of the stress response,we compared the global gene expression profiles from leaves of two soybean genotypes that display different responses to water-deficit(BR 16 and Embrapa 48,drought-sensitive and droughttolerant,respectively).After the RNA-seq analysis,a total of 5335 down-regulated and 3170 up-regulated genes were identified in the BR16.On the other hand,the number of genes differentially expressed was markedly lower in the Embrapa 48,355 up-regulated and 471 down-regulated genes.However,induction and expression of protein kinases and transcription factors indicated signaling cascades involved in the drought tolerance.Overall,the results suggest that the metabolism of pectin is differently modulated in response to drought stress and may play a role in the soybean defense mechanism against drought.This occurs via an increase of the cell wall plasticity and crosslink,which contributed to a higher hydraulic conductance(Kf)and relative water content(RWC%).The drought-tolerance mechanism of the Embrapa 48 genotype involves remodeling of the cell wall and increase of the hydraulic conductance to the maintenance of cell turgor and metabolic processes,resulting in the highest leaf RWC,photosynthetic rate(A),transpiration(E)and carboxylation(A/Ci).Thus,we concluded that the cell wall adjustment under drought is important for a more efficient water use which promoted a more active photosynthetic metabolism,maintaining higher plant growth under drought stress. | Flaviane Silva Coutinho Juliano Mendonça Rodrigues Lucas Leal Lima Rosilene Oliveira Mesquita Paola Avelar Carpinetti João Paulo Batista Machado Camilo Elber Vital Pedro Marcus Vidigal Maria Eduarda Soares Ramos Mariana Rocha Maximiano Angela Mehta Maria Goreti Almeida Oliveira Elizabeth Pacheco Batista Fontes Humberto Josuéde Oliveira Ramos | 2021 | aBIOTECH2021，2，1： | 0 |
| 18 | Influence of the Deep Cryogenic Treatment at the Stabilization of Martensitic Transformation Temperatures at the Smart Material Alloy Cu-14Al-4Ni显示文摘DCT(deep cryogenic treatment)is commonly used in industry to improve the wear resistance characteristics of steels,especially.However,there are just a few researches about the effects on non-ferrous metals.The purpose of this work was to investigate how DCT affects the properties of Cu-14Al-4Ni alloy treated at different soak time and submitted to thermomechanical cycling.A comparative experimental analysis was performed of the thermal properties of alloys obtained on a vacuum furnace,treated by DCT and thermomechanically cyclized.The results indicates that thermomechanical cycling promoted the appearance and growth of the martensitic phaseγ'1,less ductile than the martensitic phaseβ'1,which together with the induced hardening produced an increase in transformation temperatures and microhardness.The higher the number of cycles,the greater these effects.The DCT promoted an increase in the intensity of the diffraction peaks corresponding to the phaseβ'1 and the maintenance of them during the thermomechanical cycling of the material,which indicates that the DCT stabilizes the martensitic phaseβ'1 and,consequently,caused a reduction and stabilization of the martensitic transformation temperatures and the microhardness,when compared to the untreated material.The longer the soaking time of DCT,the greater these effects. | Emmanuel Pacheco Rocha Lima Marcelo Nava Pedro Cunha de Lima | 2020 | 材料科学与工程(中英文A版)2020，10，2： | 0 |
| 19 | Comparisson of Recrystallization Kinetics and Grain Growth in Polycrystalline Shape Memory Alloys显示文摘The non-ferrous SMAs(shape memory alloys)have,normally,two problems that hinder the use in industrial scale:the natural aging and grain growth.The first degrades the memory effect,while the second,observed during the alloy’s mechanical processing,modifies the phase transformation temperatures.Thus,the study of recrystallization kinetics is important for enabling the control of hardened state as a function of treatment time without allowing the exaggerated grain growth.The objective of this study is to determine the recrystallization kinetics in different SMAs(Cu-14Al-4Ni,Cu-12Al-0.5Be and Ni-42Ti),based on an empirical law of J-M-A(Johnson-Mehl-Avrami),as well as their activation energies for grain growth process according to the empirical Arrhenius law.Quantitative evaluations of the grain growth kinetics over a wide range of indicated DSC(differential scanning calorimetry)temperatures have been performed.The results show that the alloy less susceptible to aging in temperatures below the recrystallization peak is the Ni-42Ti,because it presented the highest activation energy,followed by the Cu-14Al-4Ni.The equations that describe the recrystallization kinetics follow the empirical law of J-M-A.The recrystallization kinetics accompanied by hardness variation was an important tool,working as an advisor for selection of treatment time as a function of temperature. | Marcelo Nava Emmanuel Pacheco Rocha Lima Pedro Cunha de Lima | 2020 | 材料科学与工程(中英文A版)2020，10，2： | 0 |
| 20 | Real-world disease activity and sociodemographic,clinical and treatment characteristics of moderate-to-severe inflammatory bowel disease in Brazil显示文摘BACKGROUND Understanding the treatment landscape of inflammatory bowel diseases(IBD)is essential for improving disease management and patient outcomes.Brazil is the largest Latin American country,and it presents socioeconomic and health care differences across its geographical regions.This country has the highest increase in IBD incidence and prevalence in Latin America,but information about the clinical and treatment characteristics of IBD is scarce.AIM To describe the sociodemographic,clinical,and treatment characteristics of IBD outpatients in Brazil overall and in the Southeast,South and Northeast/Midwest regions.METHODS Multicenter,cross-sectional study with a 3-year retrospective chart review component.Patients with moderate-to-severe Crohn’s disease(CD)or ulcerative colitis(UC)were consecutively enrolled between October 2016 and February 2017.Active CD at enrollment was defined as a Harvey Bradshaw Index≥8 or a CD Activity Index≥220 or a calprotectin level>200μg/g or an active result based on colonoscopy suggestive of inadequate control during the previous year;active UC was defined as a partial Mayo score≥5.Descriptive statistics were used to analyze all variables.RESULTS In a total of 407 included patients,CD was more frequent than UC,both overall(264 CD/143 UC patients)and by region(CD:UC ratios of 2.1 in the Southeast,1.6 in the South and 1.2 in the Northeast/Midwest).The majority of patients were female(54.2%of CD;56.6%of UC),and the mean ages were 45.9±13.8 years(CD)and 42.9±13.0 years(UC).The median disease duration was 10.0(range:0.5-45)years for both IBD types.At enrollment,44.7%[95%confidence interval(CI):38.7-50.7]of CD patients and 25.2%(95%CI:18.1-32.3)of UC patients presented with active disease.More than 95%of IBD patients were receiving treatment at enrollment;CD patients were commonly treated with biologics(71.6%)and immunosuppressors(67.4%),and UC patients were commonly treated with mesalazine[5-Aminosalicylic acid(5-ASA)]derivates(69.9%)and immunosuppressors(44.1%).More than 50%of the CD patients had ileocolonic disease,and 41.7%presented with stricturing disease.One-quarter of CD patients had undergone CD-related surgery in the past 3 years,and this proportion was lower in the Northeast/Midwest region(2.9%).CONCLUSION In Brazil,there are regional variations in IBD management.CD outweighs UC in both frequency and disease activity.However,one-quarter of UC patients have active disease,and most are receiving 5-ASA treatment. | Cyrla Zaltman Rogério Serafim Parra Ligia Yukie Sassaki Genoile Oliveira Santana Maria de Lourdes Abreu Ferrari Sender J Miszputen Heda M B S Amarante Roberto Luiz Kaiser Junior Cristina Flores Wilson R Catapani JoséMiguel Luz Parente Mauro Bafutto Odery Ramos Carolina D Gonçalves Isabella Miranda Guimaraes Jose J R da Rocha Marley R Feitosa Omar Feres Rogerio Saad-Hossne Francisco Guilherme Cancela Penna Pedro Ferrari Sales Cunha Tarcia NF Gomes Rodrigo Bremer Nones Mikaell Alexandre Gouvea Faria Mírian Perpétua Palha Dias Parente António S Scotton Rosana Fusaro Caratin Juliana Senra Júlio Maria Chebli | 2021 | World Journal of Gastroenterology2021，27，2： | 0 |
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