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| 1 | HLA-DRB1 allele polymorphisms in genetic susceptibility to esophageal carcinoma显示文摘AIM: To probe into the genetic susceptibility of HLA-DRB1 alleles to esophageal carcinoma in Han Chinese in Hubei Province.METHODS: HLA-DRB1 allele polymorphisms were typed by polymerase chain reaction with sequence-specific primers (PCR-SSP) in 42 unrelated patients with esophageal cancer and 136 unrelated normal control subjects and the associated HLA-DRB1 allele was measured by nucleotide sequence analysis with PCR.SAS software was used in statistics.RESULTS: Allele frequency (AF) of HLA-DRB1·0901 was significantly higher in esophageal carcinoma patients than that in the normal controls (0.2500 vs0.1397, P=0.028, the odds ratio 2.053, etiologic fraction 0.1282). After analyzed the allele nucleotide sequence of HLA-DRB1·0901 which approachs to the corresponded exon 2 sequence of the allele in genebank. There was no association between patients and controls in the rested HLA-DRB1 alleles.CONCLUSION: HLA-DRB1·0901 allele is more common in the patients with esophageal carcinoma than in the healthy controls, which is positively associated with the patients of Hubei Han Chinese. Individuals carrying HLA-DRB1·0901may be susceptible to esophageal carcinoma. | JunLin Chang-ShengDeng JieSun Xian-GongZheng XingHuang YanZhou PingXiong Ya-PingWang | 2003 | World Journal of Gastroenterology2003，9，3： | 8 |
| 2 | Antitumor immunopreventive effect in mice induced by DNA vaccine encoding a fusion protein of α-fetoprotein and CTLA4显示文摘AIM:To develop a tumor DNA vaccine encoding a fusion protein of murine AFP and CTLA4,and to study its ability to induce specific CTL response and its protective effect against AFP-producing tumor.METHODS:Murine α-fetoprotein (mAFP) gene was cloned from total RNA of Hepal-6 cells by RT-PCR.A DNA vaccine was constructed by fusion murine α-fetoprotein gene and extramembrane domain of murine CTLA4 gene.The DNA vaccine was identified by restriction enzyme analysis,sequencing and expression.EL-4 (mAFP) was developed by stable transfection of EL-4 cells with pmAFP.The frequency of cells producing IFN-γ in splenocytes harvested from the immunized mice was measured by ELISPOT.Mice immunized with DNA vaccine were inoculated with EL-4(mAFP) cells in back to observe the protective effect of immunization on tumor. On the other hand,blood samples were collected from the immunized mice to check the functions of liver and kidney.RESULTS:1.8kb mAFP cDNA was cloned from total RNA of Hepal-6 cells by RT-PCR.The DNA vaccine encoding a fusion protein of mAFP-CTLA4 was constructed and confirmed by restriction enzyme analysis,sequencing and expression.The expression of mAFP mRNA in EL-4 (mAFP) was confirmed by RT-PCR.The ELISPOT results showedthat the number of IFN-γ-producing cells in pmAFP-CTLA4 group was significantly higher than that in pmAFP,pcDNA3.1 and PBS group.The tumor volume in pmAFP-CTLA4 group was significantly smaller than that in pmAFP,pcDNA3.1 and PBS group, respectively. The hepatic and kidney functions in each group were not altered.CONCLUSION: AFP-CTLA4 DNA vaccine can stimulate potent specific CTL responses and has distinctive antitumor effect on AFP-producing tumor.The vaccine has no impact on the function of mouse liver and kidney. | GengTian Ji-LinYi PingXiong | 2004 | World Journal of Gastroenterology2004，10，2： | 5 |
| 3 | A phase Ⅱ study of modified FOLFOX as first-line chemotherapy in elderly patients with advanced gastric cancer显示文摘 | Jian guozhao Feng qiu Jian pingxiong | | 0，，： | 1 |
| 4 | Structure, composition and optical properties of Cu 2 ZnSnS 4 thin films deposited by Pulsed Laser Deposition method显示文摘 | Lin Sun Jun He Hui Kong Fangyu Yue Pingxiong Yang Junhao Chu | 2011 | Solar Energy Materials and Solar Cells2011，，10： | 1 |
| 5 | The ferroelectric thin films of SrBi_(2)Ta_(2)O_(9) prepared using pulsed laser deposition显示文摘LATELY, the ferroelectric random access memories (FRAM) have become one of the most attractive subjects in solid devices due to faster access time as dynamic RAM, the non-volatility as erasable programmable read-only memory, radiation-hardness and lower power | YANG Pingxiong, ZHENG Lirong and LIN ChengluState Key Laboratory of Functional Materials for Information, Shanghai Institute of Metallurgy, Chinese Academy of Sciences, Shanghai 200050, China | 1997 | Chinese Science Bulletin1997，42，7： | 1 |
| 6 | Dielectric properties of SrBi2Ta2O9 films in the low-temperature range显示文摘 | Yang Pingxiong Guo Ming Shi Meirong | | 0，，： | 1 |
| 7 | Analysis of TAP1 and TAP2 Polymorphism of Mother-Infant in Chinese Patients with Pre-Eclampsia显示文摘To analyze the polymorphism of TAP gene and the shared rates of alleles between mothers and their infants in Chinese patients with pre-eclampsia, TAP1 and TAP2 genotyping was performed by the amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) in 42 patients, 106 normal pregnant women, and their neonates. The allelic frequency of TAP and the alleles shared in maternal-fetus were compared and analyzed in the two groups. Our results showed that, with totally eight alleles of TAP1 and TAP2 examined in the samples, no significant difference was found in allelic frequencies between pre-eclampsia group and control group, as well as between mothers and their neonates. Similar finding was obtained in the comparison with shared alleles. In conclusion, our results do not support a role for the polymorphisms of TAP in the etiology of pre-eclampsia.Cellular & Molecular Immunology.2005;2(2):141-144. | ZhanZhang LitingJia LeiHou PingXiong XiongwenWu XuemeiWang YafeiHuang HangjunKe CaihongChang ShihongCui FeiliGong | 2005 | Cellular & Molecular Immunology2005，2，2： | 0 |
| 8 | Research Progress on Production of Chlorophyll and Other Products with Silkworm Excrement and Industrial Development over the Past 50 Years显示文摘The development of sericultural industry in the Pearl River Delta has contributed greatly to good ecological environment and economic prosperity of the people’s livelihood.Comprehensive utilization of sericulture resources can turn waste into valuable resources and expand the development approaches of sericultural industry.For example,using abundant silkworm excrement to produce chlorophyll is one of the good practices in comprehensive utilization of sericulture resources,which has leading technologies and good economic benefits.In addition to chlorophyll,the products produced from silkworm excrement can be used as the main raw materials of medicine,food,plant growth regulator and cosmetics.For instances,phytol,the important raw material for synthesis of vitamin E and vitamin K,was extracted from silkworm excrement,enabling China to become an important production base of vitamin E and vitamin K.Triacontanol extracted from silkworm excrement is a new crop growth regulator,which can increase the yield of rice and vegetable.Pectin,dietary fiber and carotenoid extracted from silkworm excrement as well can be used as natural raw material in many fields.China is the biggest manufacturer and supplier of silkworm excrement products in the world,which has a development history of about 50 years and generated high export revenue.It is believed that close combination of academic research and industry development is the only way to promote the industrial development of silkworm excrement resources. | Huang Ziran Ji Pingxiong Feng Guodong Li Guangxue Cao Yongmin Shen Fengming Zhu Xiangrui Luo Quan Pang Guozhang | 2016 | Animal Husbandry and Feed Science2016，8，3： | 0 |
| 9 | Functional Haplotypes in the Promoter of Matrix Metalloproteinase-2 Predict Risk of the Occurrence and Metastasis of Esophageal Cancer显示文摘Matrix metalloproteinase-2 (MMP-2) plays important roles in cancer development and aggression. Our previous studies revealed a strong association between the MMP-2 -1306C/T polymorphism and risk of several cancers. A novel -735C/T polymorphism in MMP-2 promoter has been identified but the function is undefined. This study examined our hypothesis that these two polymorphisms might have functional relevance and impact on risk of esophageal squamous cell carcinoma in the context of haplotype. Genotypes and haplotypes were analyzed in 527 cases and 777 controls and odds ratios (ORs) and 95% confidence intervals (CIs)were estimated by logistic regression. The function of the polymorphisms was examined by electrophoretic mobility shift assays, luciferase gene expression assays, and reverse transcriptase-PCR analyses. It was found that the -735C→T transition disrupts an Spl site and displays a lowerpromoter activity. The C_1306-C-735 haplotype had 7-fold increased luciferase expression and 3.7-fold increased MMPo2 mRNA levels in esopha-gcal tissues compared with the T-1306-T-735 haplotype. A case-control analysis revealed a 1.52-fold (95% CI=1.17-1.96) or 1.30-fold (95%CI = 1.04-1.63) excess risk of developing esophageal squamous cell carcinoma for the -1306CC or -735CC genotype carriers compared with noncarriers, respectively. A greater association was observed between elevated risk of developing esophageal squamous cell carcinoma andC-1306 or C-735 allele containing haplotypes, with the risk being highest for the C-1306-C-735 haplotype compared with the T-1306-T-735 haplotype(OR = 6.53; 95% CI = 2.78-15.33). The C-1306-C-735 haplotype was also associated with increased risk for distant metastasis of esophageal squamous cell carcinoma (OR = 3.34; 95% CI = 1.16-9.63). These findings suggest that the C-1306-C-735 haplotype in the MMpo2 promoter contributes to risk of the occurrence and metastasis of esophageal squamous cell carcinoma by increasing expression of MMP-2. | ChunyuanYu YifengZhou XiaopingMiao PingXiong WenTan DongxinLin | 2005 | 中国生物学文摘2005，19，6： | 0 |
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