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| 1 | The CRISPR/Cas revolution continues: From efficient gene editing for crop breeding to plant synthetic biology显示文摘Since the discovery that nucleases of the bacterial CRISPR(clustered regularly interspaced palindromic repeat)-associated(Cas) system can be used as easily programmable tools for genome engineering,their application massively transformed different areas of plant biology. In this review, we assess the current state of their use for crop breeding to incorporate attractive new agronomical traits into specific cultivars of various crop plants. This can be achieved by the use of Cas9/12 nucleases for double-strand break induction,resulting in mutations by non-homologous recombinatr e-tion. Strategies for performing such experiments à from Rthe design of guide RNA to the use of different transformation technologies à are evaluated. Furtherweive-more, we sum up recent developments regarding the use of nuclease-deficient Cas9/12 proteins, as DNAbinding moieties for targeting different kinds of enzyme activities to specific sites within the genome. Progress in base deamination, transcriptional induction and transcriptional repression, as well as in imaging in plants, is also discussed. As different Cas9/12 enzymes are at hand, the simultaneous application of various enzyme activities, to multiple genomic sites, is now in reach to redirect plant metabolism in a multifunctional manner and pave the way for a new level of plant synthetic biology. | Jochen Kumlehn Janine Pietralla Goetz Hensel Michael Pacher Holger Puchta | 2018 | Journal of Integrative Plant Biology2018，60，12： | 9 |
| 2 | Comparison of Copeptin, B-Type Natriuretic Peptide, and Amino-Terminal Pro-B-Type Natriuretic Peptide in Patients With Chronic Heart Failure显示文摘 | Stephanie Neuhold Martin Huelsmann Guido Strunk Brigitte Stoiser Joachim Struck Nils G. Morgenthaler Andreas Bergmann Deddo Moertl Rudolf Berger Richard Pacher | 2008 | Journal of the American College of Cardiology2008，，4： | 3 |
| 3 | Mitochondrial reactive oxygen species generation triggers inflammatory response and tissue injury associated with hepatic ischemia–reperfusion: Therapeutic potential of mitochondrially targeted antioxidants显示文摘 | Partha Mukhopadhyay B?la Horváth Zsuzsanna Zsengell?r Sándor Bátkai Zongxian Cao Malek Kechrid Eileen Holovac Katalin Erd?lyi Galin Tanchian Lucas Liaudet Isaac E. Stillman Joy Joseph Balaraman Kalyanaraman Pál Pacher | 2012 | Free Radical Biology and Medicine2012，，5： | 2 |
| 4 | Role of nitrosative stress and peroxynitrite in the pathogenesis of diabetic complications,emerging new therapeutical strategies显示文摘 | Pacher P Obrosova IG Mabley JG | 2005 | Curr Med Chem2005，12，3： | 1 |
| 5 | Relationship between neopterin and granulocyte elastase plasma levels and the severity of multiple organ failure显示文摘 | Pacher R Redl H Frass M | 1989 | Crit Care Med1989，17，2： | 1 |
| 6 | Role of the peroxynitrite-poly (ADP- ribose) polymerase pathway in human disease显示文摘 | Pacher P Szabo C | 2008 | The American Journal of Pathology2008，173，： | 1 |
| 7 | Activation of poly (ADP-ribose) polymerase contributes to the endothelial dysfunction associated with hypertension and aging显示文摘 | PACHER P MABLEY J G SORIANO F G | 2002 | Int J Mol Med2002，9，： | 1 |
| 8 | Heart failure in diabetes 显示文摘 | Resl M Hulsmann M Pacher R | 2009 | Wien Med Wochenschr2009，159，545： | 1 |
| 9 | Nitric oxide and peroxynitrite in health and disease 显示文摘 | Pacher P Beckman JS Liaudet L | 2007 | Physiol Rev2007，1，： | 1 |
| 10 | Effects of cannabinoids A ( 9 )-tetrahydrocannabinol, A ( 9 )- tetrahydrocannabinolic acid and cannabidiol in MPP ( + ) affected muSne mesencephalic cultures 显示文摘 | Moldzio R Pacher T Krewenka C | 2012 | Phytomedicine2012，19，89： | 1 |
| 11 | The endocannabinoid system as an emerging target of pharmacotherapy显示文摘 | Pacher P Bátkai S Kunos G | 2006 | Pharmacol Rev2006，58，3： | 1 |
| 12 | Dysfunction of nitric oxide mediation in isolated rat arterioles with methionine diet induced hyperhomocysteinemia显示文摘 | Ungvari Z Pacher P Rischak K | 1999 | Arterioscler Thromb Vasc Biol1999，19，8： | 1 |
| 13 | Therapeutic effects of xanthine oxidase inhibitors:renaissance half a century after the discovery of allopurinol显示文摘 | Pacher P Nivorozhkin A Szabo C | 2006 | Pharmacol Rev2006，58，1： | 1 |
| 14 | Role of peroxynitrite in the cardiovascular dysfunction of septic shock 显示文摘 | Liaudet L Rosenblatt-Velin N Pacher P | 2013 | Curr Vasc Pharmacol2013，11，2： | 1 |
| 15 | Prognostic impact of big endothelin-1 plasma concentrations compared with invasive hemodynamic evaluation in severe heart failure显示文摘 | Pacher R Stanek B Hulsmann M | 1996 | J Am Coll Cardiol1996，27，3： | 1 |
| 16 | Atrial natiuretic peptide release is more dependent on atrial filling volume than on filling pressure in chronic cogestive heart failure 显示文摘 | Globis S Frank H Pacher B | 1998 | Am Heart J1998，135，4： | 1 |
| 17 | The endocannabinoid system as an emerging target of pharmacotherapy显示文摘 | Pacher P Bátkai S Kunos G | 2006 | Pharmacol Rev2006，58，3： | 1 |
| 18 | Nitric oxide and peroxynitrite in health and disease显示文摘 | Pacher P Beckman JS Liaudet L | 2007 | Physiol Rev2007，87，1： | 1 |
| 19 | The endocannabinoid system as an emerging target of pharmacotherapy显示文摘 | Pacher P Batkai S Kunos G | 2006 | Pharmacol2006，58，3： | 1 |
| 20 | The effect of carvedilol on morbidity andmolality in patients with chronic heart failure显示文摘 | PACHER M BRISTOW M R COHN J R | 1996 | N Engl T Med1996，334，： | 1 |
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