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您的检索式：作者名="Goetz Hensel"
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| 1 | The CRISPR/Cas revolution continues: From efficient gene editing for crop breeding to plant synthetic biology显示文摘Since the discovery that nucleases of the bacterial CRISPR(clustered regularly interspaced palindromic repeat)-associated(Cas) system can be used as easily programmable tools for genome engineering,their application massively transformed different areas of plant biology. In this review, we assess the current state of their use for crop breeding to incorporate attractive new agronomical traits into specific cultivars of various crop plants. This can be achieved by the use of Cas9/12 nucleases for double-strand break induction,resulting in mutations by non-homologous recombinatr e-tion. Strategies for performing such experiments à from Rthe design of guide RNA to the use of different transformation technologies à are evaluated. Furtherweive-more, we sum up recent developments regarding the use of nuclease-deficient Cas9/12 proteins, as DNAbinding moieties for targeting different kinds of enzyme activities to specific sites within the genome. Progress in base deamination, transcriptional induction and transcriptional repression, as well as in imaging in plants, is also discussed. As different Cas9/12 enzymes are at hand, the simultaneous application of various enzyme activities, to multiple genomic sites, is now in reach to redirect plant metabolism in a multifunctional manner and pave the way for a new level of plant synthetic biology. | Jochen Kumlehn Janine Pietralla Goetz Hensel Michael Pacher Holger Puchta | 2018 | Journal of Integrative Plant Biology2018，60，12： | 9 |
| 2 | Polarized Defense Against Fungal Pathogens Is Mediated by the Jacalin-Related Lectin Domain of Modular Poaceae-Specific Proteins显示文摘模块化的蛋白质是一个进化答案优化身体上为功能与对方一起交往的蛋白质的性能。用基因、生物化学的实验的联合,我们描绘了米饭蛋白质 OsJAC1,它由领域预言了绑包含甘露糖的 oligosaccharides,和可能在 monolignols 联合的 stereoselective 工作的一个 dirigent 领域的 jacalin 相关的 lectin (JRL ) 组成。在米饭的 OsJAC1 的转基因的 overexpression 包括细菌, oomycetes,和真菌对不同病原体导致了量的用途广泛的抵抗。在大麦的这基因或它的小麦 ortholog TAJA1 的 Overexpression 对粉的霉真菌提高了抵抗。OsJAC1 的两个蛋白质领域被要求建立由单个领域的单个或联合的短暂表示显示了的抵抗。表示人工地分开了,标注荧光的蛋白质领域证明 JRL 领域为指向粉的霉穿入地点是足够的。不过, lectinand 的合作本地化 dirigent 领域发生了。种系发生的分析专门在 Poaceae 植物家庭以内揭示了 OsJAC1 的 orthologs。Dicots 由对比,仅仅与 JRL 或 dirigent 领域包含蛋白质。总的来说,我们的结果从植物作为抵抗 proteinderived 的一种新奇类型的一个代表识别 OsJAC1 为在本地病原体防卫的更好的功能的系特定的基因熔化事件。 | Denise Weidenbach Lara Esch Claudia Moller Goetz Hensel Jochen Kumlehn Caroline Hofle Ralph Huckelhoven Ulrich Schaffrath | 2016 | Molecular Plant2016，9，4： | 4 |
| 3 | Efficient generation of transgenic barley:The way forward to modulate plant-microbe interactions显示文摘 | Goetz Hensel Vladimir Valkov Jill Middlefell Williams | | 0，，： | 1 |
| 4 | Agrobacterium-mediated gene transfer to cereal crop plants:Current protocols for barley,wheat,triticale,and maize显示文摘 | Goetz Hensel Christine Kastner Sylwia Oleszczuk | | 0，，： | 1 |
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