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| 1 | Guest Editorial Semiotic Perspectives on Learning Mathematics and Communicating Mathematically显示文摘 | Adalira SáEnz–Ludlow Norma Presmeg | 2006 | Educational Studies in Mathematics (-)2006，，1： | 1 |
| 2 | Chromatographic determination of the mycotoxin patulin in fruit and fruit juices 显示文摘 | Gordon S Shephard Norma L Leggott | 2000 | Journal of Chromatographic A2000，882，： | 1 |
| 3 | HP0953-hypothetical virulence factor overexpresion and localization during Helicobacter pylori infection of gastric epithelium显示文摘BACKGROUND The high prevalence and persistence of Helicobacter pylori(H. pylori) infection, as well as the diversity of pathologies related to it, suggest that the virulence factors used by this microorganism are varied. Moreover, as its proteome contains 340hypothetical proteins, it is important to investigate them to completely understand the mechanisms of its virulence and survival. We have previously reported that the hypothetical protein HP0953 is overexpressed during the first hours of adhesion to inert surfaces, under stress conditions, suggesting its role in the environmental survival of this bacterium and perhaps as a virulence factor.AIM To investigate the expression and localization of HP0953 during adhesion to an inert surface and against gastric(AGS) cells.METHODS Expression analysis was performed for HP0953 during H. pylori adhesion. HP0953 expression at 0,3, 12, 24, and 48 h was evaluated and compared using the Kruskal-Wallis equality-of-populations rank test. Recombinant protein was produced and used to obtain polyclonal antibodies for immunolocalization. Immunogold technique was performed on bacterial sections during adherence to inert surfaces and AGS cells, which was analyzed by transmission electron microscopy. HP0953 protein sequence was analyzed to predict the presence of a signal peptide and transmembrane helices, both provided by the ExPASy platform, and using the GLYCOPP platform for glycosylation sites. Different programs, via, I-TASSER, RaptorX, and HHalign-Kbest, were used to perform three-dimensional modeling.RESULTS HP0953 exhibited its maximum expression at 12 h of infection in gastric epithelium cells.Immunogold technique revealed HP0953 localization in the cytoplasm and accumulation in some peripheral areas of the bacterial body, with greater expression when it is close to AGS cells.Bioinformatics analysis revealed the presence of a signal peptide that interacts with the transmembrane region and then allows the release of the protein to the external environment. The programs also showed a similarity with the Tip-alpha protein of H. pylori. Tip-alpha is an exotoxin that penetrates cells and induces tumor necrosis factor alpha production, and HP0953 could have a similar function as posttranslational modification sites were found;modifications in turn require enzymes located in eukaryotic cells. Thus, to be functional, HP0953 may necessarily need to be translocated inside the cell where it can trigger different mechanisms producing cellular damage.CONCLUSION The location of HP0953 around infected cells, the probable posttranslational modifications, and its similarity to an exotoxin suggest that this protein is a virulence factor. | Nancy K Arteaga-Resendiz Gerardo E Rodea Rosa María Ribas-Aparicio Alma L Olivares-Cervantes Juan Arturo Castelán-Vega Joséde Jesús Olivares-Trejo Sandra Mendoza-Elizalde Edgar O López-Villegas Christian Colín Pamela Aguilar-Rodea Alfonso Reyes-López Marcela Salazar García Norma Velázquez-Guadarrama | 2022 | World Journal of Gastroenterology2022，28，29： | 1 |
| 4 | Stability of enzymes and proteins in dried glassy systems:effect of simulated sunlight conditions显示文摘 | Carolina Schebor Norma S | 2004 | Biotechnol Prog2004，，： | 1 |
| 5 | Patulin in South African commercial apple products 显示文摘 | Norma L L Gordon S S | 2001 | Food Control2001，12，2： | 1 |
| 6 | Time course of hexachlorobenzene - induced alterations of lipid metabolima and their relation to Forphyria显示文摘 | SILVIA B D C NORMA S S MARIA C F | 1997 | Int J Biochern Cell Biol1997，，29： | 1 |
| 7 | Antimicrobial susceptibility pattern and plasmid profile in Salmonella Muenster isolated from wine and abattoir environment, Brazil显示文摘 | Norma S L Anita T Eliane M F | 2004 | Pesquisa Veteri- naria Brasileira2004，24，2： | 1 |
| 8 | Chromatographic determination of the mycotoxin patulin in fruit and fruit juices 显示文摘 | Gordon S Shephard Norma L Leggott | 2002 | Journal of Chromatographic A2002，，： | 1 |
| 9 | Quantitative determination of pneumococcal capsular polysaccharide serotype 14 using a modification of phenol - sulfuric acid method显示文摘 | Gabriela C Norma S Maria I B | 2003 | J Microbiol Meth2003，52，： | 1 |
| 10 | Oliver gastric residual volume and aspiration in critically ill patients receiving gastric feedings显示文摘 | Norma A Metheny L S Dana A | 2008 | Am J Crit Care2008，17，6： | 1 |
| 11 | Quantitative determination of pneumococcal capsuar polysaccharide serotype 14 using a modification of phenol-sulfuric acid method显示文摘 | Gabriela C Norma S Maria I B | 2003 | Journal of Microbiological Methods2003，52，： | 1 |
| 12 | Life-Threatening Cryoglobulinemia: Clinical and Immunological Characterization of 29 Cases显示文摘 | Manuel Ramos-Casals Angel Robles Pilar Brito-Zerón Norma Nardi José M. Nicolás Xavier Forns Joan Plaza Jordi Yagüe Jose M. Sánchez-Tapias Josep Font | 2006 | Seminars in Arthritis and Rheumatism2006，，3： | 1 |
| 13 | Quantitative determination of pneumococcal capsular polysaccharide serotype 14 using a modifi cation of phenol-sulfuric acid method显示文摘 | Gabriela C Norma S Maria I B | 2003 | Journal of Microbiological Methods2003，52，1： | 1 |
| 14 | Helicobacter pylori secretes the chaperonin GroEL (HSP60), which binds iron显示文摘 | Marco Antonio González-López Norma Velázquez-Guadarrama María Elena Romero-Espejel< !--Q2: Please check all affiliations and correct if necessary.--> José de Jesús Olivares-Trejo | 2013 | FEBS Letters2013，，12： | 1 |
| 15 | Species of agave with antimicrobial activity against selected pathogenic bacteria and fungi显示文摘 | ANGELES V S JULIA V SANTOS G NORMA H AZUCENA O CATALINA R | 2008 | World J Microbiol Biotcchnol2008，24，： | 1 |
| 16 | Nitric: oxide as a mediator of fructose 1, 6-bisphosphate protection in galactosamine-indueed hepatotoxicity in rats显示文摘 | Norma A David C Sergio S | 2013 | Nitric Oxide2013，28，1： | 1 |
| 17 | Chromatographic determination of the mycotoxin patulin in fruit and fruit juices显示文摘 | Gordon S Norma L | 2000 | Journal of Chromatography A2000，882，： | 1 |
| 18 | RAPD and ISSR markers indicate diminished gene flow due to recent fragmentation of Polylepis australis woodlands in central Argendna显示文摘 | NORMA J ANA S JUAN R D JULIO D R DANIEL R ISABELL H | 2008 | Biockemical Systematics and Ecology2008，36，： | 1 |
| 19 | Patulin in South African commercial apple products显示文摘 | Norma L Gordon S | 2001 | Food Control2001，，12： | 1 |
| 20 | One-pot synthesis of N,N,N',N'-tetrasubstituted ureas and oxomalonamides by oxidative carbonylation of lith- ium amides at atmospheric pressure显示文摘 | Norma S N Elizabeth S L Daniel G P | 1990 | Synthesis1990，，10： | 1 |
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