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| 1 | Dual-action penems and carbapenems显示文摘 | Alfred J C Scott L D Norma K D | 1992 | J Med Chem1992，35，10： | 1 |
| 2 | Chromatographic determination of the mycotoxin patulin in fruit and fruit juices 显示文摘 | Gordon S Shephard Norma L Leggott | 2000 | Journal of Chromatographic A2000，882，： | 1 |
| 3 | HP0953-hypothetical virulence factor overexpresion and localization during Helicobacter pylori infection of gastric epithelium显示文摘BACKGROUND The high prevalence and persistence of Helicobacter pylori(H. pylori) infection, as well as the diversity of pathologies related to it, suggest that the virulence factors used by this microorganism are varied. Moreover, as its proteome contains 340hypothetical proteins, it is important to investigate them to completely understand the mechanisms of its virulence and survival. We have previously reported that the hypothetical protein HP0953 is overexpressed during the first hours of adhesion to inert surfaces, under stress conditions, suggesting its role in the environmental survival of this bacterium and perhaps as a virulence factor.AIM To investigate the expression and localization of HP0953 during adhesion to an inert surface and against gastric(AGS) cells.METHODS Expression analysis was performed for HP0953 during H. pylori adhesion. HP0953 expression at 0,3, 12, 24, and 48 h was evaluated and compared using the Kruskal-Wallis equality-of-populations rank test. Recombinant protein was produced and used to obtain polyclonal antibodies for immunolocalization. Immunogold technique was performed on bacterial sections during adherence to inert surfaces and AGS cells, which was analyzed by transmission electron microscopy. HP0953 protein sequence was analyzed to predict the presence of a signal peptide and transmembrane helices, both provided by the ExPASy platform, and using the GLYCOPP platform for glycosylation sites. Different programs, via, I-TASSER, RaptorX, and HHalign-Kbest, were used to perform three-dimensional modeling.RESULTS HP0953 exhibited its maximum expression at 12 h of infection in gastric epithelium cells.Immunogold technique revealed HP0953 localization in the cytoplasm and accumulation in some peripheral areas of the bacterial body, with greater expression when it is close to AGS cells.Bioinformatics analysis revealed the presence of a signal peptide that interacts with the transmembrane region and then allows the release of the protein to the external environment. The programs also showed a similarity with the Tip-alpha protein of H. pylori. Tip-alpha is an exotoxin that penetrates cells and induces tumor necrosis factor alpha production, and HP0953 could have a similar function as posttranslational modification sites were found;modifications in turn require enzymes located in eukaryotic cells. Thus, to be functional, HP0953 may necessarily need to be translocated inside the cell where it can trigger different mechanisms producing cellular damage.CONCLUSION The location of HP0953 around infected cells, the probable posttranslational modifications, and its similarity to an exotoxin suggest that this protein is a virulence factor. | Nancy K Arteaga-Resendiz Gerardo E Rodea Rosa María Ribas-Aparicio Alma L Olivares-Cervantes Juan Arturo Castelán-Vega Joséde Jesús Olivares-Trejo Sandra Mendoza-Elizalde Edgar O López-Villegas Christian Colín Pamela Aguilar-Rodea Alfonso Reyes-López Marcela Salazar García Norma Velázquez-Guadarrama | 2022 | World Journal of Gastroenterology2022，28，29： | 1 |
| 4 | Patulin in South African commercial apple products 显示文摘 | Norma L L Gordon S S | 2001 | Food Control2001，12，2： | 1 |
| 5 | Antimicrobial susceptibility pattern and plasmid profile in Salmonella Muenster isolated from wine and abattoir environment, Brazil显示文摘 | Norma S L Anita T Eliane M F | 2004 | Pesquisa Veteri- naria Brasileira2004，24，2： | 1 |
| 6 | Modelling of batch produc- tion systems using Petri nets with dynamic tokens显示文摘 | ERNESTO L M NORMA V P | 2005 | Math- ematics and Computers in Simulation2005，67，3： | 1 |
| 7 | Chromatographic determination of the mycotoxin patulin in fruit and fruit juices 显示文摘 | Gordon S Shephard Norma L Leggott | 2002 | Journal of Chromatographic A2002，，： | 1 |
| 8 | Oliver gastric residual volume and aspiration in critically ill patients receiving gastric feedings显示文摘 | Norma A Metheny L S Dana A | 2008 | Am J Crit Care2008，17，6： | 1 |
| 9 | Bcl-2 protects against oxidative stress while inducing premature senescence显示文摘 | Norma E L o pez-Diazguerrero Hugo L 6 pez-Araiza | 2006 | Free Radical Biology and Medicine2006，40，7： | 1 |
| 10 | Quantifying Self-Report Measures’ Overestimation of Mobility Scores Postarthroplasty/Invited Commentary/Invited Commentary/Author Response显示文摘 | Stratford Paul W Kennedy Deborah M Maly Monica R MacIntyre Norma J Mizner Ryan L Pua Yong Hao Bennell Kim L | 2010 | Physical Therapy2010，，9： | 1 |
| 11 | Pollen Tube Growth in Mandarin显示文摘 | Norma L G A Ediberto A G Ana M C G | 2004 | Revista Fitotecnia Mexicana2004，27，2： | 1 |
| 12 | Metabolic profiles of sunflower genotypes with contrasting response to Sclerotinia sclerotiorum infection显示文摘 | Peluffo L Lia V Troglia C Maringolo C Norma P Escande A Esteban Hopp H Lytovchenko A Fernie A R Heinz R Carrari F | 2010 | Phytochemistry2010，71，1： | 1 |
| 13 | 显示文摘 | Wang Chiahao Maria L Auad Norma E | 2008 | Journal of Applied Polymer Science2008，109，4： | 1 |
| 14 | Spect roelectrochemist ry of palladium hexaeyanoferrate films on platinum substrates 显示文摘 | REYNALDO O L ROBERTO R NORMA R D T | 2003 | Journal of Electroanalytical Chemistry2003，544，： | 1 |
| 15 | Biochemical properties of mammalian neutral sphingomyelinase2 and its role in sphingolipid metabolism显示文摘 | Chiara L Yusuf AH | 2003 | J Biol Chem2003，278，13： | 1 |
| 16 | Chromatographic determination of the mycotoxin patulin in fruit and fruit juices显示文摘 | Gordon S Norma L | 2000 | Journal of Chromatography A2000，882，： | 1 |
| 17 | Patulin in South African commercial apple products显示文摘 | Norma L Gordon S | 2001 | Food Control2001，，12： | 1 |
| 18 | Diabetes Mellitus and Anal Sphincter Pressures: An Experimental Model in Rats显示文摘 | Henrique Sarubbi Fillmann M.D. Suzana Llessuy Ph.D. Cláudio A. Marroni M.D. Ph.D. Lúcio S. Fillmann M.D. Ph.D. Norma Possa Marroni Ph.D | 2007 | Diseases of the Colon & Rectum2007，，4： | 1 |
| 19 | Using waist circumference as a screening tool to identify colombian subjects at cardiovascular risk显示文摘 | Maritza Perez Juan P Casas Luz A Cubillos-Garzón Norma C Serrano Federico Silva Carlos A Morillo Patricio López-Jaramillo | 2003 | European Journal of Cardiovascular Prevention & Rehabilitation2003，，5： | 1 |
| 20 | One-pot synthesis of N,N,N',N'-tetrasubstituted ureas and oxomalonamides by oxidative carbonylation of lith- ium amides at atmospheric pressure显示文摘 | Norma S N Elizabeth S L Daniel G P | 1990 | Synthesis1990，，10： | 1 |
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