|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | CD69 expression on airway eosinophils and airway inflammation in a murine model of asthma显示文摘Background Asthma is a chronic airway disease with inflammation characterized by physiological changes (airway hyper-responsiveness, AHR) and pathological changes (inflammatory cells infiltration and mucus production). Eosinophils play a key role in the allergic inflammation. But the causative relationship between eosinophils and airway inflammation is hard to prove. One of the reasons is lack of activation marker of murine eosinophils. We investigated the expression of CD69 on murine eosinophils in vitro, the relationship between the expression of CD69 on eosinophils from peripheral blood and bronchoalveolar lavage fluid and on airway inflammation in asthmatic mice. Methods Eosinophils from peripheral blood of IL-5 transgenic mice (NJ.1638) were purified. Mice were divided into five groups: wild type mice sensitized and challenged with saline (WS group), wild type mice sensitized and challenged with ovalbumin (WO group), IL-5-/- mice sensitized and challenged with saline and transferred with purified eosinophils (ISE group), IL-5-/- mice sensitized and challenged with OVA and transferred with purified eosinophils (IOE group), IL-5-/- mice sensitized and challenged with OVA and transferred with purified eosinophils, pretreated with anti CD4 monoclonal antibody (IOE+antiCD4mAb group). IL-5-/- mice were sensitized with OVA at day 0 and day 14, then challenged with OVA aerosol. On days 24, 25, 26 and 27 purified eosinophils were transferred intratracheally to IL-5-/- mice. On day 28, blood and BALF were collected and CD69 expression on eosinophils measured by flowcytometry. Results Purified eosinophils did not express CD69. But eosinophils cultured with PMA+MA, IFN-γ, IL-5 or GM-CSF expressed CD69 strongly. Eosinophils from blood of WO, WS group did not express CD69 at all. The numbers of eosinophils in BALF of WO group, IOE group, ISE group and IOE+antiCD4mAb group were significantly higher than in mice of WS group which did not have eosinophils at all. CD69 expression on eosinophils in BALF of IOE and WO groups was strong. Eosinophils in BALF of ISE and IOE+antiCDmAb groups did not express CD69. The mucus production result was similar to CD69 expression. There were eosinophils infiltration in lung slides of all groups except WS group. Conclusion Activation in airway of eosinophils could directly lead to airway inflammation. | WANG Hui-ying SHEN Hua-hao James J Lee Nancy A Lee | 2006 | Chinese Medical Journal2006，，23： | 8 |
| 2 | CD69的表达在小鼠嗜酸细胞的活化与凋亡中的作用显示文摘目的:观察在体内、体外不同条件下小鼠嗜酸细胞(EOS)表面CD69的表达与细胞存活率,探讨CD69的表达在小鼠EOS的活化、凋亡中的作用。方法:提纯IL-5高分泌转基因小鼠外周血中的EOS,测定其表面CD69的表达,体外以PMA+MA刺激EOS,在1 h、12 h、18 h、24 h测定细胞表面CD69的表达与细胞存活率;分别以1μg/L的IL-4、IL-5、IL-12、IL-13、IFN-γ、GM-CSF培养EOS18 h后测定细胞的存活率与表面CD69的表达。制备小鼠哮喘模型,观察CD69在小鼠BALF、外周血EOS中的表达。结果:新鲜提纯的小鼠外周血EOS不表达CD69,PMA+MA刺激的EOS在1 h后即有CD69的表达,12 h表达至高峰,至少持续24 h以上;但细胞的存活率快速下降。不同细胞因子对EOS的培养均可诱导CD69的表达,其中IL-13、IFN-γ、GM-CSF对此影响明显,且GM-CSF可显著抑制EOS的凋亡;哮喘小鼠外周血EOS无CD69的表达,而BALF中EOS可有CD69的表达。结论:静止小鼠EOS表面不表达CD69,但在体内、体外不同条件下激活的EOS表面均有CD69的表达;同时,体外实验显示CD69的表达与细胞凋亡密切相关。结果提示CD69既可作为EOS激活的表面标记物,同时又可诱导EOS的凋亡,这为哮喘治疗提供新的思路。 | 汪慧英 James J Lee Nancy A Lee | 2009 | 中国病理生理杂志2009，25，1： | 7 |
| 3 | Gastric cancer in Alaska Native people: A cancer health disparity显示文摘AIM To evaluate recent trends in gastric cancer incidence, response to treatment, and overall survival among Alaska Native(AN) people. METHODS A retrospective analysis of the Alaska Native Medical Center patient database was performed. Patient history, clinical, pathological, response to treatment and patient outcomes were collected from one-hundred and thirty-two AN gastric cancer patients. The Surveillance, Epidemiology and End Result database 18 was used to collect comparison United States non-Hispanic White(NHW) and AN gastric cancer patient data between 2006-2014.RESULTS AN gastric cancer patients have a higher incidence rate, a poorer overall survival, and are diagnosed at a significantly younger age compared to NHW patients. AN patients differ from NHW patients in greater prevalence of non-cardia, diffuse subtype, and signet ring cell carcinomas. AN females were more likely to be diagnosed with later stage cancer, stage IV, compared to AN males. Diminished overall survival was observed among AN patients with increasing stage, O+ blood type, < 15 lymph nodes examined at resection, and no treatment. This study is the first report detailing the clinicopathologic features of gastric cancer in AN people with outcome data.CONCLUSION Our findings confirm the importance of early detection, treatment, and surgical resection for optimizing AN patient outcomes. Further research on early detection markers are warranted. | Holly A Martinson Nancy J Shelby Steven R Alberts Matthew J Olnes | 2018 | World Journal of Gastroenterology2018，24，25： | 6 |
| 4 | Soy-based renoprotection显示文摘Chronic kidney disease(CKD) is a significant publich ealth problem as risk factors such as advanced age, obesity, hypertension and diabetes rise in the global po-pulation. Currently there are no effective pharmacologic treatments for this disease. The role of diet is important for slowing the progression of CKD and managing symptoms in later stages of renal insufficiency. While low protein diets are generally recommended, maintaining adequate levels of intake is critical for health. There is an increasing appreciation that the source of protein may also be important. Soybean protein has been the most extensively studied plant-based protein in subjects with kidney disease and has demonstrated renal protective properties in a number of clinical studies. Soy protein consumption has been shown to slow the decline in estimated glomerular filtration rate and significantly improve proteinuria in diabetic and non-diabetic patients with nephropathy. Soy's beneficial effects on renal function may also result from its impact on certain phy-siological risk factors for CKD such as dyslipidemia, hypertension and hyperglycemia. Soy intake is also associated with improvements in antioxidant status and systemic inflammation in early and late stage CKD pati-ents. Studies conducted in animal models have helped to identify the underlying molecular mechanisms that may play a role in the positive effects of soy protein on renal parameters in polycystic kidney disease, metabolically-induced kidney dysfunction and age-associated prog-ressive nephropathy. Despite the established relationship between soy and renoprotection, further studies are needed for a clear understanding of the role of the cellular and molecular target(s) of soy protein in main-taining renal function. | Nancy J Mc Graw Elaine S Krul Elizabeth Grunz-Borgmann Alan R Parrish | 2016 | World Journal of Nephrology2016，5，3： | 5 |
| 5 | Interprofessional, multiple step simulation course improves pediatric resident and nursing staff management of pediatric patients with diabetic ketoacidosis显示文摘AIM To investigate the use of a multidisciplinary, longitudinal simulation to educate pediatric residents and nurses on management of pediatric diabetic ketoacidosis.METHODS A multidisciplinary, multiple step simulation course was developed by faculty and staff using a modified Delphi method from the Pediatric Simulation Center and pediatric endocrinology department. Effectiveness of the simulation for the residents was measured with a pre- and post-test and a reference group not exposed to simulation. A follow up post-test was completed 3-6 mo after the simulation. Nurses completed a survey regarding the education activity. RESULTS Pediatric and medicine-pediatric residents(n = 20) and pediatric nurses(n = 25) completed the simulation course. Graduating residents(n = 16) were used as reference group. Pretest results were similar in the control and intervention group(74% ± 10% vs 76% ± 15%, P = 0.658). After completing the intervention, participants improved in the immediate post-test in comparison to themselves and the control group(84% ± 12% post study; P < 0.05). The 3-6 mo follow up post-test results demonstrated knowledge decay when compared to their immediate post-test results(78% ± 14%, P = 0.761). Residents and nurses felt the interdisciplinary and longitudinal nature of the simulation helped with learning.CONCLUSION Results suggest a multidisciplinary, longitudinal simulation improves immediate post-intervention knowledge but important knowledge decay occurs, future studies are needed to determine ways to decrease this decay. | Linnea M Larson-Williams Amber Q Youngblood Dawn Taylor Peterson J Lynn Zinkan Marjorie L White Hussein Abdul-Latif Leen Matalka Stephen N Epps Nancy M Tofil | 2016 | World Journal of Critical Care Medicine2016，5，4： | 4 |
| 6 | Primer containing dimethylaminododecyl methacrylate kills bacteria impregnated in human dentin blocks显示文摘Antibacterial dimethylaminododecyl methacrylate(DMADDM) was recently synthesized. The objectives of this study were to:(1)investigate antibacterial activity of DMADDM-containing primer on Streptococcus mutans impregnated into dentin blocks for the first time, and(2) compare the antibacterial efficacy of DMADDM with a previous quaternary ammonium dimethacrylate(QADM).Scotchbond Multi-Purpose(SBMP) bonding agent was used. DMADDM and QADM were mixed into SBMP primer. Six primers were tested: SBMP control primer P, P+2.5% DMADDM, P+5% DMADDM, P+7.5% DMADDM, P+10% DMADDM, and P+10% QADM.S. mutans were impregnated into human dentin blocks, and each primer was applied to dentin to test its ability to kill bacteria in dentinal tubules. Bacteria in dentin were collected via a sonication method, and the colony-forming units(CFU) and inhibition zones were measured. The bacterial inhibition zone of P+10% DMADDM was 10 times that of control primer(Po0.05). CFU in dentin with P+10% DMADDM was reduced by three orders of magnitude, compared with control. DMADDM had a much stronger antibacterial effect than QADM, and antibacterial efficacy increased with increasing DMADDM concentration. Dentin shear bond strengths were similar among all groups(P40.1). In conclusion, antibacterial DMADDM-containing primer was validated to kill bacteria inside dentin blocks, possessing a much stronger antibacterial potency than the previous QADM. DMADDM-containing bonding agent was effective in eradicating bacteria in dentin, and its efficacy was directly proportional to DMADDM mass fraction. Therefore, DMADDM may be promising for use in bonding agents as well as in other restorative and preventive materials to inhibit bacteria. | Chen Chen Lei Cheng Michael D Weir Nancy J Lin Sheng Lin-Gibson Xue-Dong Zhou Hockin HK Xu | 2016 | International Journal of Oral Science2016，8，4： | 4 |
| 7 | Drugs Associated with Hepatotoxicity and their Reporting Frequency of Liver Adverse Events in VigiBase(TM)显示文摘 | Suzuki Ayako Andrade Raul J Bjornsson Einar Lucena M Isabel Lee William M Yuen Nancy A Hunt Christine M Freston James W | 2010 | Drug Safety2010，，6： | 2 |
| 8 | American society of echocardiography guidelines and standards for performance of the fetal echocardiogram显示文摘 | Jack Rychik Nancy Ayres Bettina Cuneo Nina Gotteiner Lisa Hornberger Philip J Spevak Mary Van Der Veld | 2004 | Journal of the American Society of Echocardiography2004，，7： | 2 |
| 9 | Global malaria mortality between 1980 and 2010: a systematic analysis显示文摘 | Christopher JL Murray Lisa C Rosenfeld Stephen S Lim Kathryn G Andrews Kyle J Foreman Diana Haring Nancy Fullman Mohsen Naghavi Rafael Lozano Alan D Lopez | 2012 | The Lancet2012，，9814： | 2 |
| 10 | Human ICE/CED-3 Protease Nomenclature显示文摘 | Emad S Alnemri David J Livingston Donald W Nicholson Guy Salvesen Nancy A Thornberry Winnie W Wong Junying Yuan | 1996 | Cell1996，，2： | 2 |
| 11 | No Significant Association of 14 Candidate Genes With Schizophrenia in a Large European Ancestry Sample: Implications for Psychiatric Genetics显示文摘 | Sanders Alan R Duan Jubao Levinson Douglas F Shi Jianxin He Deli Hou Cuiping Burrell Gregory J Rice John P Nertney Deborah A Olincy Ann Rozic Pablo Vinogradov Sophia Buccola Nancy G Mowry Bryan J Freedman Robert Amin Farooq Black Donald W | 2008 | The American Journal of Psychiatry2008，，4： | 1 |
| 12 | Development of a quantitative method for the analysis of tobacco-specific nitrosa- mines in mainstream cigarette smoke using isotope dilution liquid chromatography/electrospray ionization tandem mass spectrometry 显示文摘 | Karl A W Nancy H F Fossett J E | 2005 | Analytical Chemistry2005，77，： | 1 |
| 13 | Prevention and Control of Influenza with Vaccines Recommendations of the Advisory Committee on Immunization Practices (ACIP), 2010显示文摘 | Fiore Anthony E Uyeki Timothy M Broder Karen Finelli Lyn Euler Gary L Singleton James A Iskander John K Wortley Pascale M Shay David K Bresee Joseph S Cox Nancy J | 2010 | MMWR. Morbidity and Mortality Weekly Report2010，，： | 1 |
| 14 | Intraoperative ultrasound显示文摘 | Junji Machi Andrew J Oishi Nancy L Furumoto Robert H Oishi | 2004 | Surgical Clinics of North America2004，，4： | 1 |
| 15 | Bone sialoprotein mRNA expression and ultrastructural localization in fetal porcine calvarial bone: comparisons with osteopontin 显示文摘 | CHEN J MCKEE M D NANCI A | 1994 | Histochem J1994，26，1： | 1 |
| 16 | Factors contributing to inshopping behavior in rural trade areas:implications for local retailers显示文摘 | Miller Nancy J and Kean Rita C | 1997 | Journal of Small Business Management1997，35，2： | 1 |
| 17 | Baker Trace element composition of municipal waters in the Unite States:A comparison of ICP-AES and ICP-MS methods显示文摘 | Nancy J Miller-Ihli Scott A | 2004 | Journal of Food Composition and Analysis2004，14，： | 1 |
| 18 | Established and emerging plasma biomarkers in the prediction of first atherothrombotic events显示文摘 | Ridker PM Nancy J Douglas E | 2004 | Circulation2004，109，1： | 1 |
| 19 | DNA microarray analysis of the expression of the genes encoding the major enzymes in ethanolproduction during glucose and xylose cofermentation by metabolically engineered Saccharomyces yeast显示文摘 | Miroslav S Howard J E Nancy W Y | 2003 | Science2003，，33： | 1 |
| 20 | The behavioral determinants of exercise:implications for physical activity interventions显示文摘 | Nancy E S Robert W J | 2000 | Annual Reviews2000，，20： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员