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| 1 | Overview of Citrin Deficiency:SLC25A13 Mutations and the Frequency显示文摘Citrin deficiency,autosomal recessive disorder,caused by mutation of SLC25A13 gene on chromosome 7q21.3 has two major phenotypes:neonatal intrahepatic cholestatic hepatitis(NICCD)and adult-onset type Ⅱ citrullinemia(CTLN2).So far,we have identified 52 SLC25A13 mutations and diagnosed the patients not only in Japan(166 CTLN2 and 238 NICCD) but also in other countries.We have detected 76 Chinese,13 Korean and 15 Vietnamese patients with the same mutations as Japanese,and 13 patients(from Israel,UK,USA or Czech)with mutations different from those found in Japanese,indicating a wide distribution of citrin deficiency.DNA diagnoses of 13 known SLC25A13 mutations revealed that the carrier frequency was high in East Asian populations:Chinese(73/4 600=1/63),Japanese(21/1 372=1/65) and Korean(25/2 690=1/108),suggesting that near by 100 000 East Asians are homozygotes.It is important to find out patients with citrin deficiency,to treat them,and to prevent onset of severe CTLN2. | Keiko Kobayashi Miharu Ushtkai Yuan - Zong Song Hong - Zhi Gao Jian - Sheng Sheng Ayako Tabata Furnihiko Okumura Sayaka lkeda Takeyori Saheki | 2008 | 实用儿科临床杂志2008，23，20： | 26 |
| 2 | Comparative study: Vonoprazan and proton pump inhibitors in Helicobacter pylori eradication therapy显示文摘AIM To compare the effectiveness and safety of vonoprazan-based therapy with proton pump inhibitor(PPI)-based therapies to treat Helicobacter pylori(H. pylori).METHODS We retrospectively analysed data from first-line(vonoprazan or PPI with 200 mg clarithromycin and 750 mg amoxicillin twice daily for 7 d)(n = 1353) and second-line(vonoprazan or PPI with 250 mg metronidazole and 750 mg amoxicillin twice daily for 7 d)(n = 261) eradication treatments for H. pylori- positive patients with associated gastrointestinal diseases from April 2014 to December 2015 at Hattori Clinic, Japan. The primary endpoint was the eradication rate, which was assessed with a full analysis set. The secondary endpoints were adverse events and related factors.RESULTS After the first-line treatments, the eradication rates for vonoprazan, esomeprazol, rabeprazole, and lansoprazole were 87.9%(95%CI: 84.9%-90.5%), 71.6%(95%CI: 67.5%-75.5%), 62.9%(95%CI: 52.0%-72.9%), and 57.3%(95%CI: 50.4%-64.1%), respectively. The vonoprazan eradication rate was significantly higher than that of the PPIs(P < 0.01). Interestingly, smoking did not affect the H. pylori eradication rate in the vonoprazan group(P = 0.34), whereas it decreased the rates in the PPI groups(P = 0.013). The incidence of adverse events in the vonop-razan group was not different from the PPI group(P = 0.054), although the vonoprazan group exhibited a wider range of adverse events. Vonoprazan-based triple therapy was highly effective as a second-line treatment, with an eradication rate similar to that of PPI-based therapy.CONCLUSION Vonoprazan might be superior to PPIs in first-line H. pylori therapy, particularly for smokers. However, caution is required due to possible adverse events. | Kouichi Sakurai Hiroko Suda Yumi Ido Takayuki Takeichi Ayako Okuda Kiwamu Hasuda Masahiro Hattori | 2017 | World Journal of Gastroenterology2017，23，4： | 16 |
| 3 | NF-κB and ERK-signaling pathways contribute to the gene expression induced by cag PAI-positive-Helicobacter pylori infection显示文摘AIM: To elucidate the sequential gene expression profile in AGS cells co-cultured with wild-type Hellcobacter pylori (Hpylon) as a model of H pylori-infected gastric epithelium, and to further examine the contribution of cag-pathogenicity islands (cagPAI)-coding type Ⅳ secretion system and the two pathways, nuclear factor kappa B (NF-κB) and extracellular signal-regulated kinases (ERK) on wild-type H pylori-induced gene expression. METHODS: Gene expression profiles induced by H pylori were evaluated in AGS gastric epithelial cells using cDNA microarray, which were present in the 4 600 independent clones picked up from the human gastric tissue. We also analyzed the contribution of NF-κB and ERK signaling on H pylori-induced gene expression by using inhibitors of specific signal pathways. The isogenic mutant with disrupted capE (△cagE) was used to elucidate the role of capPAI-encoding type Ⅳ secretion system in the gene expression profile. RESULTS: According to the expression profile, the genes were classified into four clusters. Among them, the clusters characterized by continuous upregulation were most conspicuous, and it contained many signal transducer activity-associated genes. The role of cagPAI on cultured cells was also investigated using isogenic mutant cagE, which carries non-functional cagPAI. Then the upregulation of more than 80% of the induced genes (476/566) was found to depend on cagPAI. Signal transducer pathway through NF-κB or ERK are the major pathways which are known to be activated by cagPAI-positive H pylori. The role of these pathways in the whole signal activation by cagPAI- positive H pylori was analyzed. The specific inhibitors against NF-κB or ERK pathway blocked the activation of gene expression in 65% (367/566) or 76% (429/566) of the genes whose activation appealed to depend on cagPAI. CONCLUSION: These results suggest that more than half of the genes induced by cagPAI-positive H pylori depend on NF-κB and ERK signaling activation, and these pathways may play a role in the gene expression induced by host-bacterial interaction which may associate with H pylori-related gastro-duodenal diseases. | Wataru Shibata Yoshihiro Hirata Haruhiko Yoshida Motoyuki Otsuka Yujin Hoshida Keiji Ogura Shin Maeda Tomoya Ohmae Ayako Yanai Yuzo Mitsuno Naohiko Seki Takao Kawabe Masao Omata | 2005 | World Journal of Gastroenterology2005，11，39： | 12 |
| 4 | Hepatitis B virus infection and alcohol consumption显示文摘Hepatocellular carcinoma(HCC) is the most common type of primary liver cancer, and the second most common cause of cancer deaths worldwide. The top three causes of HCC are hepatitis B virus(HBV),hepatitis C virus(HCV), and alcoholic liver disease. Owing to recent advances in direct-acting antiviral agents, HCV can now be eradicated in almost all patients. HBV infection and alcoholic liver disease are expected, therefore, to become the leading causes of HCC in the future. However, the association between alcohol consumption and chronic hepatitis B in the progression of liver disease is less well understood than with chronic hepatitis C. The mechanisms underlying the complex interaction between HBV and alcohol are not fully understood, and enhanced viral replication, increased oxidative stress and a weakened immune response could each play an important role in the development of HCC. It remains controversial whether HBV and alcohol synergistically increase the incidence of HCC. Herein, we review the currently available literature regarding the interaction of HBV infection and alcohol consumption on disease progression. | ayako iida-ueno masaru enomoto akihiro tamori norifumi kawada | 2017 | World Journal of Gastroenterology2017，23，15： | 11 |
| 5 | Estimation of aboveground biomass using in situ hyperspectral measurements in five major grassland ecosystems on the Tibetan Plateau显示文摘Aims There are numerous grassland ecosystem types on the Tibetan Plateau.These include the alpine meadow and steppe and degraded alpine meadow and steppe.This study aimed at developing a method to estimate aboveground biomass(AGB)for these grasslands from hyperspectral data and to explore the feasibility of applying air/satellite-borne remote sensing techniques to AGB estimation at larger scales.Methods We carried out a field survey to collect hyperspectral reflectance and AGB for five major grassland ecosystems on the Tibetan Plateau and calculated seven narrow-band vegetation indices and the vegetation index based on universal pattern decomposition(VIUPD)from the spectra to estimate AGB.First,we investigated correlations between AGB and each of these vegetation indices to identify the best estimator of AGB for each ecosystem type.Next,we estimated AGB for the five pooled ecosystem types by developing models containing dummy variables.At last,we compared the predictions of simple regression models and the models containing dummy variables to seek an ecosystem type-independent model to improve prediction of AGB for these various grassland ecosystems from hyperspectral measurements.Important findings When we considered each ecosystem type separately,all eight vegetation indices provided good estimates of AGB,with the best predictor of AGB varying among different ecosystems.When AGB of all the five ecosystems was estimated together using a simple linear model,VIUPD showed the lowest prediction error among the eight vegetation indices.The regression models containing dummy variables predicted AGB with higher accuracy than the simple models,which could be attributed to the dummy variables accounting for the effects of ecosystem type on the relationship between AGB and vegetation index(VI).These results suggest that VIUPD is the best predictor of AGB among simple regression models.Moreover,both VIUPD and the soil-adjusted VI could provide accurate estimates of AGB with dummy variables integrated in regression models.Therefore,ground-based hyperspectral measurements are useful for estimating AGB,which indicates the potential of applying satellite/airborne remote sensing techniques to AGB estimation of these grasslands on the Tibetan Plateau. | Miaogen Shen Yanhong Tang Julia Klein Pengcheng Zhang Song Gu Ayako Shimono Jin Chen | 2008 | Journal of Plant Ecology2008，1，4： | 10 |
| 6 | Immunological Effects of Silica and Asbestos显示文摘Silicosis patients(SILs) and patients who have been exposed to asbestos develop not only respiratory diseases but also certain immunological disorders. In particular,SIL sometimes complicates autoimmune diseases such as systemic scleroderma,rheumatoid arthritis(known as Caplan syndrome) ,and systemic lupus erythematoses. In addition,malignant complications such as lung cancer and malignant mesothelioma often occurr in patients exposed to asbestos,and may be involved in the reduction of tumor immunity. Although silica-induced disorders of autoimmunity have been explained as adjuvant-type effects of silica,more precise analyses are needed and should reflect the recent progress in immunomolecular findings. A brief summary of our investigations related to the immunological effects of silica/asbestos is presented. Recent advances in immunomolecular studies led to detailed analyses of the immunological effects of asbestos and silica. Both affect immuno-competent cells and these effects may be associated with the pathophysiological development of complications in silicosis and asbestos-exposed patients such as the occurrence of autoimmune disorders and malignant tumors,respectively. In addition,immunological analyses may lead to the development of new clinical tools for the modification of the pathophysiological aspects of diseases such as the regulation of autoimmunity or tumor immunity using cell-mediated therapies,various cytokines,and molecule-targeting therapies. In particular,as the incidence of asbestos-related malignancies is increasing and such malignancies have been a medical and social problem since the summer of 2005 in Japan,efforts should be focused on developing a cure for these diseases to eliminate nationwide anxiety. | Takemi Otsuki Megumi Maeda Shuko Murakami Hiroaki Hayashi Yoshie Miura Masayasu Kusaka Takashi Nakano Kazuya Fukuoka Takumi Kishimoto Fuminori Hyodoh Ayako Ueki Yasumitsu Nishimura | 2007 | Cellular & Molecular Immunology2007，4，4： | 9 |
| 7 | Patterns of plant diversity at high altitudes on the Qinghai-Tibetan Plateau显示文摘To describe the biodiversity patterns of plants along an altitudinal gradient on the Qinghai-Tibetan Plateau and to clarify the bias in plant specimen records at high altitude.Methods We conducted a large-scale investigation of vegetation at a wide range of altitudes,focusing on a high-altitudinal range(3200-5200 m)at different locations on the Qinghai-Tibetan Plateau.We then compared the altitudinal distribution of plant species obtained from our field investigation with that in plant specimen records from published sources and an online database.Important Findings Our data provide evidence that altitude plays a large role in regulating species composition on the Qinghai-Tibetan Plateau.We could not,however,detect a clear relationship between altitude and species richness,although a weak monotonically increasing trend of richness was detected with increasing altitude.According to specimen records,most species have been sampled at a wide range of altitudes,and the average range of 145 species is>2000 m.Despite this wide range,more than half of the species we observed were at higher altitudes than the specimen records indicate.High-altitude areas have probably been so poorly sampled that only a small fraction of the resident species has been recorded.This study clearly shows the regional bias of specimen records in the Qinghai-Tibetan Plateau. | Ayako Shimono Huakun Zhou Haihua Shen Mitsuru Hirota Toshiyuki Ohtsuka Yanhong Tang | 2010 | Journal of Plant Ecology2010，3，1： | 6 |
| 8 | Altitudinal variation of ecosystem CO_(2) fluxes in an alpine grassland from 3600 to 4200 m显示文摘Aims Recent studies have recognized the alpine grasslands on the Qinghai-Tibetan plateau as a significant sink for atmospheric CO_(2).The carbon-sink strength may differ among grassland ecosystems at various altitudes because of contrasting biotic and physical environments.This study aims(i)to clarify the altitudinal pattern of ecosystem CO_(2) fluxes,including gross primary production(GPP),daytime ecosystem respiration(Redaytime)and net ecosystem production(NEP),during the period with peak above-ground biomass;and(ii)to elucidate the effects of biotic and abiotic factors on the altitudinal variation of ecosystem CO_(2) fluxes.Methods Ecosystem CO_(2) fluxes and abiotic and biotic environmental factors were measured in an alpine grassland at four altitudes from 3600 to 4200 m along a slope of the Qilian Mountains on the northwestern Qinghai-Tibetan Plateau during the growing season of 2007.We used a closed-chamber method combined with shade screens and an opaque cloth to measure several carbon fluxes,GPP,Redaytime and NEP,and factors,light-response curve for GPP and temperature sensitivity of Redaytime.Above-and below-ground biomasses and soil C and N contents at each measurement point were also measured.Important Findings(i)Altitudinal pattern of ecosystem CO_(2) fluxes:The maximum net ecosystem CO_(2) flux(NEPmax),i.e.the potential ecosystem CO_(2) sink strength,was markedly different among the four altitudes.NEPmax was higher at the highest and lowest sites,ap proximately7.460.9 and6.760.6 lmol CO_(2) m^(-2)s^(-1)(mean 6 standard error),respectively,but smaller at the intermediate altitude sites(3800 and 4000 m).The altitudinal pattern of maximum gross primary production was similar to that of NEPmax.The Redaytime,however,was significantly higher at the lowest altitude(3.460.3 lmol CO_(2) m^(-2)s^(-1))than at the other three altitudes.(ii)Altitudinal variation of vegetation biomass:The aboveground biomass was higher at the highest altitude(154627 g DW m2)than at the other altitudes,which we attribute mainly to the large biomass in cushion plants at the highest altitude.The small above-ground biomass at the lower altitudes was probably due to heavy grazing during the growing season.(iii)Features of ecosystem CO_(2) fluxes:Redaytime and GPP were positively correlated with above-ground biomass.The low ratio of Redaytime to GPP at either the measurement point or the site level suggests that CO_(2) uptake efficiency tends to be higher at higher altitudes,which indicates a high potential sink strength for atmospheric CO_(2) despite the low temperature at high altitudes.The results suggest that the effect of grazing intensity on ecosystem carbon dynamics,partly by decreasing vegetation biomass,should be clarified further. | Mitsuru Hirota Pengcheng Zhang Song Gu Mingyuan Du Ayako Shimono Haihua Shen Yingnian Li Yanhong Tang | 2009 | Journal of Plant Ecology2009，2，4： | 6 |
| 9 | Protective effects of oral glutathione on fasting-induced intestinal atrophy through oxidative stress显示文摘AIM To determine whether oral glutathione(GSH)administration can alleviate the effects of fasting-induced intestinal atrophy in the small intestinal mucosa. METHODS Rats were divided into eight groups.One group was fed ad libitum,another was fed ad libitum and received oral GSH,and six groups were administrated saline(SA)or GSH orally during fasting.Mucosal height,apoptosis,and cell proliferation in the jejunum were histologically evaluated.i NOS protein expression(by immunohistochemistry),nitrite levels(by high performance liquid chromatography,as a measure of NO production),8-hydroxydeoxyguanosine formation(by ELISA,indicating ROS levels),glutathione/oxidized glutathione(GSH/GSSG)ratio(by enzymatic colorimetric detection),andγ-glutamyl transpeptidase(Ggt1)mR NA levels in the jejunum(by semi-quantitative RT-PCR)were also estimated. RESULTS O r a l G S H a d m i n i s t r a t i o n w a s d e m o n s t r a t e d t o drastically reduce fasting-induced intestinal atrophy in the jejunum.In particular,jejunal mucosal height was enhanced in GSH-treated animals compared to SA-treated animals[527.2±6.9 for 50 mg/kg GSH,567.6±5.4 for 500 mg/kg GSH vs 483.1±4.9(μm),P<0.01at 72 h].This effect was consistent with decreasing changes in GSH-treated animals compared to SA-treated animals for iN OS protein staining[0.337±0.016for 50 mg/kg GSH,0.317±0.017 for 500 mg/kg GSH vs 0.430±0.023(area of staining part/area of tissue),P<0.01 at 72 h]and NO[2.99±0.29 for 50 mg/kg GSH,2.88±0.19 for 500 mg/kg GSH vs 5.34±0.35(nmol/g tissue),P<0.01 at 72 h]and ROS[3.92±0.46for 50 mg/kg GSH,4.58±0.29 for 500 mg/kg GSH vs6.42±0.52(8-OHdG pg/μg DNA),P<0.01,P<0.05at 72 h,respectively]levels as apoptosis mediators in the jejunum.Furthermore,oral GSH administration attenuated cell proliferation decreases in the fasting jejunum[182.5±1.9 for 500 mg/kg GSH vs 155.8±3.4(5-Brd U positive cells/10 crypts),P<0.01 at 72h].Notably,both GSH concentration and Ggt1 m RNA expression in the jejunum were also attenuated in rats following oral administration of GSH during fasting as compared with fasting alone[0.45±0.12 vs 0.97±0.06(nmol/mg tissue),P<0.01;1.01±0.11 vs 2.79±0.39(Ggt1 m RNA/Gapdh m RNA),P<0.01 for 500 mg/kg GSH at 48 h,respectively]. CONCLUSION Oral GSH administration during fasting enhances jejunal regenerative potential to minimize intestinal mucosal atrophy by diminishing fasting-mediated ROS generation and enterocyte apoptosis and enhancing cell proliferation. | Hiroyuki Uchida Yukari Nakajima Kazuo Ohtake Junta Ito Masahiko Morita Ayako Kamimura Jun Kobayashi | 2017 | World Journal of Gastroenterology2017，23，36： | 5 |
| 10 | Non-bismuth quadruple therapy for first-line Helicobacter pylori eradication:A randomized study in Japan显示文摘AIM:To find the way to improve the eradication rate of first-line therapy in Japanese patients.METHODS:We prospectively compared the effectiveness of 7-d quadruple therapy to standard 7 d triple therapy in Japanese patients infected with Helicobacter pylori(H.pylori).One hundred and nineteen patients were randomly assigned to receive 7-d non-bismuth quadruple therapy with lansoprazole,amoxicillin,clarithromycin and metronidazole(LACM7) or 7-d triple therapy with lansoprazole,amoxicillin and clarithromycin(LAC7).After three months,H.pylori status was analyzed by 13C-urea breath test.Incidence rates of adverse events were evaluated by use of questionnaires.RESULTS:By intention-to-treat(ITT) analysis,the eradication rate in the LACM7 group was 94.9%,which was significantly higher than the LAC7 group(68.3%,P < 0.001).Per protocol analysis also showed a significantly higher eradication rate in the LACM7 group(98.3%) than the LAC7 group(73.2%,P < 0.001).Nevertheless,the incidence of serious adverse events did not differ between the two groups(RR:1.10,95% CI:0.70-1.73,P = 0.67).CONCLUSION:Seven day non-bismuth quadruple therapy(LACM7) was superior to standard 7-d triple therapy(LAC7) for first-line eradication. | Ayako Yanai Kei Sakamoto Masao Akanuma Keiji Ogura Shin Maeda | 2012 | World Journal of Gastrointestinal Pharmacology and Therapeutics2012，3，1： | 5 |
| 11 | No Significant Effects of Ethyl-eicosapentanoic Acid on Histologic Features of Nonalcoholic Steatohepatitis in a Phase 2 Trial显示文摘 | Arun J. Sanyal Manal Abdelmalek Ayako Suzuki William Cummings Mario Chojkier | 2014 | Gastroenterology2014，，： | 4 |
| 12 | Evaluation of a multiplex PCR assay for detection of cytomegalovirus in stool samples from patients with ulcerative colitis显示文摘AIM: To evaluate a multiplex PCR assay for the detection of bacterial and viral enteropathogens in stool samples from patients with ulcerative colitis(UC). METHODS: We prospectively analyzed 300 individuals, including immunocompetent patients, immunocompromised patients, and patients with UC. Stool samples were collected from the recto-sigmoid region of the colon by endoscopy. The samples were qualitatively analyzed for bacterial and viral enteropathogens with a multiplex PCR assay using a Seeplex® Kit. Additional clinical and laboratory data were collected from the medical records. RESULTS: A multiplex PCR assay detected 397 pathogens(191 bacteria and 206 viruses) in 215 samples(71.7%). The most frequently detected bacteria were Escherichia coli H7, 85(28.3%); followed by Aeromonas spp., 43(14.3%); and Clostridium perfringens, 36(12.0%) samples. The most prevalent viruses were Epstein-Barr virus(EBV), 90(30.0%); followed by human herpes virus-6(HHV-6), 53(17.7%); and cytomegalovirus(CMV), 37(12.3%) samples. The prevalence rate of CMV infection was significantly higher in the immunocompromised group than in the immunocompetent group(P < 0.01). CMV infection was more common in patients with UC(26/71; 36.6%)than in the immunocompetent patients excluding UC(6/188; 3.2%)(P < 0.01). CMV infection was more prevalent in UC active patients(25/58; 43.1%) than in UC inactive patients(1/13; 7.7%)(P < 0.05). Among 4 groups which defined by the UC activity and immunosuppressive drugs, the prevalence rate of CMV infection was highest in the UC active patients with immunosuppressive drugs(19/34; 55.8%). EpsteinBarr virus(EBV) infection was more common in the immunocompromised patients excluding UC(18/41; 43.9%) than in the immunocompetent patients excluding UC(47/188; 25.0%)(P < 0.05). The simultaneous presence of CMV and EBV and/or HHV6 in UC active patients(14/58; 24.1%) was greater than in immunocompromised patients excluding UC(5/41; 12.2%)(P < 0.05). CONCLUSION: The multiplex PCR assay that was used to analyze the stool samples in this study may serve as a non-invasive approach that can be used to exclude the possibility of CMV infection in patients with active UC who are treated with immunosuppressive therapy. | Saifun Nahar Atsushi Iraha Akira Hokama Ayako Uehara Gretchen Parrott Tetsuya Ohira Masatoshi Kaida Tetsu Kinjo Takeshi Kinjo Tetsuo Hirata Nagisa Kinjo Jiro Fujita | 2015 | World Journal of Gastroenterology2015，21，44： | 4 |
| 13 | 太阳紫外辐射增加对材料的作用显示文摘合成高分子,天然生物高分子以及其它一些商品材料都要受到太阳紫外辐射(UV)的有害作用。这些材料,特别是塑料在需要经常曝露于日光的环境下的应用,只有利用光稳定剂和(或)表面处理对它们进行保护才有可能。因此,由于部分臭氧减少引起的日光UV含量的任何增高都将增加这些材料的光降解速率,减少它们在户外的应用寿命。这种由日光中UV辐射增加引起的破坏的性质和程度可用作用光谱(action spectra)进行定量表征。虽然在文献上报道了几个高分子的作用光谱,现有资料常不足以对增加的破坏进行可靠的估计。高分子材料的配方,所使用的破坏判据乃至解释数据的方式都常常能影响结果。然而,从已有的资料可清楚看出,短波UV-B过程是引起曝露于日光下的高分子的光破坏的主要原因,这些破坏包括从变色直到整体机械性能的丧失。在许多情况下这些变化仍未有清楚的分子水平的解释。可以用在高分子配方中加入更多量常用光稳定剂的方法来减轻日光中增加的UV含量的作用。但是,这个方法包含了以下假设:a)在日光光谱改变了的情况下这些光稳定剂仍继续有效;b)这些稳定剂曝露于富UV的日光下自身是光稳定的;c)在足够低的深度下它们仍足够有效以达到经济的目的。尚缺乏有关这方面的实验资料。正在进行的研究,特别是有关高分子在极端环境条件下曝露的研究,可能会使这些问题变得更加清晰。用光稳定性更好的塑料和其它材料来代替原来的材料也是一个引人注意的解决方法。上述两种解决方法都将增加应用的塑料产品的价格。随着塑料大量取代传统的材料在许多方面的应用,这是需要考虑的重要问题,尤其在发展中国家更是如此。 | Anthony L.Andrady Mohamed B.Amin S.Haleem Hamid 胡兴洲 Ayako Torikai | 1995 | 人类环境杂志1995，24，3： | 4 |
| 14 | 伴放线放线杆菌菌落生长形态变化的观察显示文摘目的 观察伴放线放线杆菌 (Actinobacillusactinomycetemcomitans,Aa)从粗糙型到光滑型的转变过程 ,识别Aa在实验室传代过程中出现的不同生长形态。方法 从牙周炎患者龈下菌斑中分离出的原代菌株 8株 ,应用固体及液体培养基连续传代 ,液体培养每次传代的同时接种固体培养基观察相应的菌落形态。结果 液体培养获得 3株光滑型转变株。菌落的变化从粘附的小菌落到沉淀的大菌落到完全的均匀生长 ,转化过程大约需要 7~ 8代。在这一过程中相应传至固体培养基上生长的Aa从粘附的半透明的小菌落变大、不透明并失去粘附的特性 ,又随着边缘的扩散变为扁平 ,透明度也增加 ;与此同时内部的星形结构逐渐变简单、变小 ,最后消失。固体培养未获得典型的转变株。结论 Aa从粗糙型到光滑型的转变是一个菌落湿度逐渐增加 ,体积逐渐增大 ,并逐渐失去内部结构的过程。这一过程至少可以看到半透明突起的粗糙型、不透明突起的光滑型和近乎透明的扁平光滑型 | 王者玲 杨圣辉 李金路 Nobuko Maeda Tomoko Ohshima Ayako Takao | 2003 | 中华口腔医学杂志2003，38，1： | 3 |
| 15 | A unique case of collagenous colitis presenting as protein-losing enteropathy successfully treated with prednisolone显示文摘A 76-year-old woman with a 5-mo history of recurrent diarrhea and generalized edema was admitted to our hospital. Colonoscopy revealed edematous mucosa,and histopathological examination was compatible with collagenous colitis. Protein leakage from the colon,particularly in the ascending portion,was identified on 99mTc-human serum albumin scintigraphy. Collagenous colitis associated with protein-losing enteropathy (PLE) without small bowel disease was diagnosed. Prednisolone treatment ameliorated diarrhea and hypoproteinemia. Collagenous colitis should be included in the differential diagnosis of chronic diarrhea with hypoproteinemia for appropriate management. | Soichi Sano Keiko Yamagami Ayako Tanaka Minako Nishio Tomoyuki Nakamura Yuki Kubo Takeshi Inoue Wataru Ueda Kiyotaka Okawa Katsunobu Yoshioka | 2008 | World Journal of Gastroenterology2008，14，39： | 3 |
| 16 | Endoscopic Submucosal Dissection of Esophageal Squamous Cell Neoplasms显示文摘 | Mitsuhiro Fujishiro Naohisa Yahagi Naomi Kakushima Shinya Kodashima Yosuke Muraki Satoshi Ono Nobutake Yamamichi Ayako Tateishi Yasuhito Shimizu Masashi Oka Keiji Ogura Takao Kawabe Masao Ichinose Masao Omata | 2006 | Clinical Gastroenterology and Hepatology2006，，6： | 2 |
| 17 | Outcomes of Endoscopic Submucosal Dissection for Colorectal Epithelial Neoplasms in 200 Consecutive Cases显示文摘 | Mitsuhiro Fujishiro Naohisa Yahagi Naomi Kakushima Shinya Kodashima Yosuke Muraki Satoshi Ono Nobutake Yamamichi Ayako Tateishi Masashi Oka Keiji Ogura Takao Kawabe Masao Ichinose Masao Omata | 2007 | Clinical Gastroenterology and Hepatology2007，，6： | 2 |
| 18 | Hematogenous umbilical metastasis from colon cancer treated by palliative single-incision laparoscopic surgery显示文摘Sister Mary Joseph’s nodule(SMJN)is a rare umbilical nodule that develops secondary to metastatic cancer.Primary malignancies are located in the abdomen or pelvis.Patients with SMJN have a poor prognosis.An 83-year-old woman presented to our hospital with a1-month history of a rapidly enlarging umbilical mass.Endoscopic findings revealed advanced transverse colon cancer.computer tomography and fluorodeoxyglucose-positron emission tomography revealed tumors of the transverse colon,umbilicus,right inguinal lymph nodes,and left lung.The feeding arteries and drainage veins for the SMJN were the inferior epigastric vessels.Imaging findings of the left lung tumor allowed for identification of the primary lung cancer,and a diagnosis of advanced transverse colon cancer with SMJN and primary lung cancer was made.The patient underwent local resection of the SMNJ and subsequent single-site laparoscopic surgery involving right hemicolectomy and paracolic lymph node dissection.Intra-abdominal dissemination to the mesocolon was confirmed during surgery.Histopathologically,the transverse colon cancer was confirmed to be moderately differentiated tubular adenocarcinoma.We suspect that SMJN may occur via a hematogenous pathway.Although chemotherapy for colon cancer and thoracoscopic surgery for the primary lung cancer were scheduled,the patient and her family desired home hospice.Seven months after surgery,she died of rapidly growing lung cancer. | Tomohide Hori Noriyuki Okada Masaya Nakauchi Shuji Hiramoto Ayako Kikuchi-Mizota Masahisa Kyogoku Fumitaka Oike Hidemitsu Sugimoto Junya Tanaka Yoshiki Morikami Kaori Shigemoto Toyotsugu Ota Masanobu Kaneko Masato Nakatsuji Shunji Okae Takahiro Tanaka Daigo Gunji Akira Yoshioka | 2013 | World Journal of Gastrointestinal Surgery2013，5，10： | 2 |
| 19 | The relationship between percentage of organic matter in substrate and plant growth in extensive green roofs显示文摘 | Ayako Nagase Nigel Dunnett | 2011 | Landscape and Urban Planning2011，，2： | 2 |
| 20 | Drugs Associated with Hepatotoxicity and their Reporting Frequency of Liver Adverse Events in VigiBase(TM)显示文摘 | Suzuki Ayako Andrade Raul J Bjornsson Einar Lucena M Isabel Lee William M Yuen Nancy A Hunt Christine M Freston James W | 2010 | Drug Safety2010，，6： | 2 |
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