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109篇 您的检索式:作者名="Murchie"
    题名 作者 年代 出处 被引量
1神经干细胞体外分化抗原表达的研究显示文摘目的:观察体外神经干细胞向神经元分化过程中标志性抗原的表达,为进一步研究神经干细胞体外增殖分化的影响因素提供参照。方法:取12~14天胎鼠大脑皮层,体外培养神经干细胞;于第1次传代后第3天将培养的神经干细胞置于含有分化培养液的培养皿中进行分化;于不同分化时间点(1、4、7、10和14天)采用免疫细胞化学方法检测神经巢蛋白(nestin)、SOX2、doublecortin(DCX)、Tuj1、神经元核心抗原(NeuN)各抗原的表达。结果:nestin和SOX2在开始时表达量非常近似,几乎在所有的细胞中两者均同时表达,随时间的推移,两者表达量均逐渐下降,但SOX2表达时间相对nestin持续更长;DCX表达量在分化1周内较高且变化不大,随后开始下降,至分化14天时仅有少数细胞呈DCX阳性;Tuj1在分化7天时已有少量表达,随后逐渐增加,分化14天时有38.27%的细胞表达Tuj1;在贴壁分化的1周内未见NeuN阳性细胞,但至分化14天时,Ne-uN阳性细胞占21.11%;在贴壁分化第1天时即有大量GFAP阳性细胞,其表达量随分化时间延长而逐渐下降,但下降趋势较nestin缓和,至分化14天时仍有大量GFAP阳性细胞。结论:神经干细胞体外分化过程中抗原的表达是一个相互交错的过程,未分化状态标志性抗原表达的减少与分化状态标志性抗原表达的增加互相对应。刘罡 吴毅 贾杰 汪洋 胡永善 Alastair I H Murchie 2009中国运动医学杂志2009,28,1:4
2运动训练在缺血性脑梗死大鼠神经干细胞移植治疗中的作用显示文摘目的探讨运动训练对缺血性脑梗死大鼠神经干细胞移植后神经功能和缺血脑区超微结构的影响。方法建立Sprague-Dawley大鼠大脑中动脉缺血/再灌注模型,随机分为脑缺血对照组(对照组)、电动跑台训练组(运动组)、神经干细胞移植组(移植组)、神经干细胞移植联合运动训练组(联合组),每组6或10只。术后第5天将超顺磁氧化铁(SPIO)标记的神经干细胞移植到缺血侧纹状体区。术后第6天开始,运动组和联合组大鼠给予定量的电动跑台运动训练,运动训练期间对4组大鼠进行定期的神经功能评估。运动4周后处死大鼠,透射电镜观察SPIO标记的神经干细胞在宿主脑内存活迁徙以及宿主脑区超微结构变化情况。结果与对照组比较,运动训练2周后运动组和联合组的神经功能评分,差异有统计学意义(P〈0.05或0.01),移植组差异无统计学意义(P〉0.05)。联合组可见SPIO标记的神经干细胞密度较大,迁徙也相对广泛,宿主缺血脑区超微结构优于其他各组。结论运动训练可以促进神经干细胞移植对脑梗死大鼠的治疗作用,但其具体的机制还有待进一步研究。刘罡 贾杰 吴毅 胡永善 汪洋 于惠贤 张峰 Alastair I H Murchie 2008中华物理医学与康复杂志2008,30,11:4
3Acclimation of Photosythesis to Irradiance and Spectral Quality in British Plant Species: Chlorophyll Content, Photosynthetic Capacity and Habitat Preference 显示文摘Murchie EH Horton P 1997Plant Cell and Environment1997,20,:2
4Novel de novo mutations of the interleukin-10 receptor gene lead to infantile onset inflammatory bowel disease显示文摘Cheng Hiang Lee Peter Hsu Brigitte Nanan Ralph Nanan Melanie Wong Kevin J. Gaskin Rupert W. Leong Ryan Murchie Aleixo M. Muise Michael O. Stormon 2014Journal of Crohn’s and Colitis2014,,:2
5Cost effectiveness of nurse led secondary prevention clinics for coronary heart disease in primary care :follow up of a randomised controlled trial显示文摘Raftery JP Yao GL Murchie P 2005BMJ2005,330,7493:1
6Higher activity of the inducible nitric oxide synthase contributes to very early onset infam- matory bowel disease 显示文摘Dhillon SS Mastropaolo LA Murchie R 2014Clin Transl Gastroenterol2014,5,1:1
7High pressure processing of shellfish:A review of microbiological and other quality aspects显示文摘MURCHIE L W KERRY J P LINTON M 2005Innovative Food Science and Emerging Technologies2005,,6:1
8Acclimation of photosynthesis to ir-radiance and spectral quality in british plant species: chlorophyllcontent photosynthetic capacity and habitat preference显示文摘MURCHIE E H HORTON P 1997Plant Cell Environment1997,20,:1
9Color Variations on Eros from NEAR Multispectral Imaging 显示文摘Murchie S Robinson M Clark B 2002Icarus2002,155,1:1
10Interactions between senescence and leaf orientatin determine in situ patterns of photosynthesis and photoinhibition in field-grown rice 显示文摘Murchie EH Chen YZ Hubbart S 1999Plant Physiology1999,119,:1
11The crystal structure of a parallel-stranded guanine tetraplex at 0.95? resolution 1 1 Edited by R.Huber显示文摘Kathryn Phillips Zbyszek Dauter Alastair I.H. Murchie David M.J. Lilley Ben Luisi 1997Journal of Molecular Biology1997,,1:1
12Agriculture and the new challenges for photosynthesis research显示文摘MURCHIE E H PINTO M HORTON P 2009New Phytolo- gist2009,181,3:1
13Developing a community- based intervention to improve quality of life in people with colorec- tal cancer: a complex intervention development study 显示文摘Gray NM Allan JL Murchie P 2013BMJ Open2013,3,00:1
14Chlorophyll fluorescenceanalysis: a guide to good practice and understanding some newapplications显示文摘MURCHIE E H LAWSON T 2013Journal of Experimental Botany2013,64,13:1
15Chlorophyll fluorescence analysis: a guide to good practice and understanding some new applica- tions显示文摘Murchie E H Lawson T 2013J Exp Bot2013,64,13:1
16Spectral evidence for hydrated salts in recurring slope lineae on Mars 显示文摘OJHA L WILHELM M B MURCHIE S L 2015Nature Geoscience2015,8,:1
17Are there associations between grain-filling rate and photosynthesis in the flag leaves of filed-grown rice?显示文摘Murchie E H Yang J Hubbart S 2002Journal of Experimental Botany2002,53,:1
18The high-resolution crystal structure of a parallel-stranded guanine tetraplex显示文摘Laughlan G Murchie A I H Norman D G 1994Science1994,265,:1
19Agriculture and the new challenges for photosynthesis research显示文摘Murchie E.H Pinto M Horton P 0,,03:1
20High pressure processing of shellfish: A review of microbiologicaland other quality aspects 显示文摘Murchie L W Cruz-Romero M Kerry J P 2005Innovative Food Science and Emerging Technologies2005,,6:1
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