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| 1 | A randomized controlled clinical trial on the treatment of Thymosin-a1 versus interferon-α in patients with hepatitis B显示文摘INTRODUCTIONChronic hepatitis B virus (HBV) infection is a serious problem because of its world wide distribution and possible adverse sequelae ,such as cirrhosis and hepatocellular carcinoma .The World Health Organization estimates that HBV has infected mord than 350 million people worldwide ,and up to 20% of them will become chromic carricrs and will be at significant risk for cirrhosis and HCC .The ultimate goal of the therapy for chronic hepatitis B is to prevent progression to cirrhosis and to prevent development of HCC. | Jing You Lin Zhuang Bao Zhang Tang Wei Bo Yang Su Ying Ding Wu Li Rong Xue Wu Hong Li Zhang Yan Mei Zhang Shao Ming Yan Lu Zhang ~1Department of Infectious Diseases,The First Affiliated Hospital of Kunming Medical College,Kunming 650032,Yunnan Province,China ~2Departrnent of Hepatology,Kunming Third Municipal People’s Hospital,Kunming 650041,Yunnan Province,China | 2001 | World Journal of Gastroenterology2001，7，3： | 48 |
| 2 | Activation of gibberellin 2-oxidase 6 decreases active gibberellin levels and creates a dominant semi-dwarf phenotype in rice (Oryza sativa L.)显示文摘Gibberellin (GA) 2-oxidase plays a key role in the GA catabolic pathway through 2β-hydroxylation.In the present study,we isolated a CaMV 35S-enhancer activation tagged mutant,H032.This mutant exhibited a dominant dwarf and GA-deficient phenotype,with a final stature that was less than half of its wild-type counterpart.The endogenous bioactive GAs are markedly decreased in the H032 mutant,and application of bioactive GAs (GA3 or GA4) can reverse the dwarf phenotype.The integrated T-DNA was detected 12.8 kb upstream of the OsGA2ox6 in the H032 genome by TAIL-PCR.An increased level of OsGA2ox6 mRNA was detected at a high level in the H032 mutant,which might be due to the enhancer role of the CaMV 35S promoter.RNAi and ectopic expression analysis of OsGA2ox6 indicated that the dwarf trait and the decreased levels of bioactive GAs in the H032 mutant were a result of the up-regulation of the OsGA2ox6 gene.BLASTP analysis revealed that OsGA2ox6 belongs to the class III of GA 2-oxidases,which is a novel type of GA2ox that uses C20-GAs (GA12 and/or GA53) as the substrates.Interestingly,we found that a GA biosynthesis inhibitor,paclobutrazol,positively regulated the OsGA2ox6 gene.Unlike the over-expression of OsGA2ox1,which led to a high rate of seed abortion,the H032 mutant retained normal flowering and seed production.These results indicate that OsGA2ox6 mainly affects plant stature,and the dominant dwarf trait of the H032 mutant can be used as an efficient dwarf resource in rice breeding. | Jian Huang Ding Tang Yi Shen Baoxiang Qin Lilan Hong Aiqing You Ming Li Xin Wang Hengxiu Yu Minghong Gu Zhukuan Cheng | 2010 | Journal of Genetics and Genomics2010，37，1： | 36 |
| 3 | 5-Hydroxymethylcytosine signatures in circulating cell-free DNA as diagnostic biomarkers for human cancers显示文摘象 5-methylcytosine (5mC ) 和 5-hydroxymethylcytosine (5hmC ) 那样的 DNA 修正是知道在哺乳动物影响全球基因表示的 epigenetic 标记。在人的染色体,与基因表示的靠近的关联和高化学的稳定性给他们的流行,这些 DNA epigenetic 标记能为癌症用作理想的 biomarkers 诊断。利用一种高度敏感、选择的化学标记技术,我们这里报导在传播没有房间的 DNA ( cfDNA )并且在从最近与 colorectal 诊断的 260 个病人的一个队收集的配对的肿瘤和邻近的纸巾的 genomic DNA ( gDNA )的 5hmC 的染色体宽的介绍,胃,胰腺,从 90 个健康个人的肝或甲状腺癌症和正常纸巾。5hmC 主要在与开的染色质和容许的 histone 修正重合的 transcriptionally 活跃的区域被散布。柔韧的联系癌症的 5hmC 签名在 cfDNA 被识别为特定的癌症类型是特征的。传播 cfDNA 的 5hmC 底 biomarkers colorectal 和胃的癌症是高度预兆的并且比常规 biomarkers 优异并且比得上从织物活体检视的 5hmC biomarkers。因此,这新策略能从血样品的分析为癌症的诊断和预后导致有效、最低限度地侵略的方法的发展。 | Wenshuai Li Xu Zhang Xingyu Lu Lei You Yanqun Song Zhongguang Luo Jun Zhang Ji Nie Wanwei Zheng Diannan Xu Yaping Wang Yuanqiang Dong Shulin Yu Jun Hong Jianping Shi Hankun Hao Fen Luo Luchun Hua Peng Wang Xiaoping Qian Fang Yuan Lianhuan Wei Ming Cui Taiping Zhang Quan Liao Menghua Dai Ziwen Liu Ge Chen Katherine Meckel Sarbani Adhikari Guifang Jia Marc B Bissonnette Xinxiang Zhang Yupei Zhao Wei Zhang Chuan He Jie Liu | 2017 | Cell Research2017，27，10： | 30 |
| 4 | Transduction of Fas gene or Bcl-2 antisense RNA sensitizes cultured drug resistant gastric cancer cells to chemotherapeutic drugs显示文摘INTRODUCTIONChemotherapyisoneofthemajormethodsintumortreatment,butitoftendoesnotworkduetomultidrugresistance(MDR).Recentstudi... | XIAO Bing, SHI Yong Quan, ZHAO Yan Qiu, YOU Han, WANG Zuo You, LIU Xian Ling, YIN Fang, QIAO Tai Dong and FAN Dai Ming | 1998 | World Journal of Gastroenterology1998，4，5： | 24 |
| 5 | Study of angiogenesis induced by metastatic and non-metastatic liver cancer by corneal micropocket model in nude mice显示文摘METHODSCornealmicropocketswerecreatedinnudemice.Tumortissuesandlivertissueswereimplantedintothecornealmicropockets.Angiogenes... | SUN Hui Chuan, LI Xiao Ming, XUE Qiong, CHEN Jun, GAO Dong Mei and TANG Zhao You | 1999 | World Journal of Gastroenterology1999，5，2： | 17 |
| 6 | Role of CyclinD1 and CDK4 in the Carcinogenesis Induced by Silica显示文摘Objective To study the role of cyclinD1 and CDK4 in malignant transformation of human fetal lung diploid fibroblast cell line(2BS) induced by silica. Methods Recombination vectors with sense and antisense pXJ41-cyclinD1 and pXJ41-CDK4 were constructed, and then transfected into the malignant transformed cells induced by silica, respectively. At the same time, pXJ41-neo was used as the control. Results During the progress of the malignant transformation of 2BS cells induced by silica, cyclinD1 and CDK4 were overexpressed. Antisense RNA suppressed cyclinD1 and CDK4 gene expression in the antisense pXJ41-cyclinD1 and pXJ41-CDK4 transfected cells. Antisense RNA led to cell cycle arrest, resulting in lengthened G1 phase (the percentages of cells in the G1 phase changed from 45.1% to 52.7% and 58.0% for cyclinD1 and CDK4 transfected cells, respectively), and eventually attenuated the increase of the proliferation of malignant transformed cells induced by silica. Compared with malignant transformed cells induced by silica, cells transfected with antisense pXJ41-cyclinD1 and pXJ41-CDK4 showed obviously reduced growth rates. On the 8th day, the suppression rates were 58.69 and 77.43% (the growth rate of malignant transformed cells induced by silica was 100%), doubling time changed from 21.0 h to 31.4 h and 21.0 h to 42.7 h, respectively, the growth capacities on soft agar of cells transfected by antisense pXJ41-cyclinD1 and pXJ41-CDK4 decreased obviously. Conclusion CyclinD1 and CDK4 play an important role in maintaining transformed phenotype of the cancer cells. | KE-XIA YAN BING-CI LIU XIANG-LIN SHI BAO-RONG YOU MING XU | 2005 | Biomedical and Environmental Sciences2005，18，5： | 14 |
| 7 | Two new triterpenoid saponins from Lonicera macranthoides显示文摘二新 triterpenoid saponins 把 lonimacranthoide 称为 IV (1 ) , lonimacranthoide V (2 ) 从忍冬 macranthoides Hand.-Mazz 的花芽被孤立。(Caprifoliaceae ) 。他们作为 aglycone 有 hederagenin。Lonimacranthoide IV (1 ) 是 hederagenin 的 C-23 上的稀罕 chlorogenic 酸酉旨 acylated。Lonimacranthoide IV 是新 sulfated triterpenoid saponin。saponins 的结构被化学、光谱的方法建立。 | Yu Chen Yu Shan You Yi Zhao Qi Zhi Wang Ming Wang Xu Feng Jing Yu Liang | 2012 | Chinese Chemical Letters2012，23，3： | 14 |
| 8 | Overview of 5G security technology显示文摘The 5 th-generation mobile communication system(5 G) has higher security requirements than previous systems. Accordingly, international standard organizations, operators, and equipment manufacturers are focusing extensively on 5 G security technology. This paper analyzes the security requirements of 5 G business applications, network architecture, the air interface, and user privacy. The development trends of5 G security architecture are summarized, with a focus on endogenous defense architecture, which represents a new trend in 5 G security development. Several incremental 5 G security technologies are reviewed, including physical layer security, lightweight encryption, network slice security, user privacy protection, and block chain technology applied to 5 G. | Xinsheng JI Kaizhi HUANG Liang JIN Hongbo TANG Caixia LIU Zhou ZHONG Wei YOU Xiaoming XU Hua ZHAO Jiangxing WU Ming YI | 2018 | Science China(Information Sciences)2018，61，8： | 14 |
| 9 | Uptake of HIV Self-testing among Men Who have Sex with Men in Beijing, China: a Cross-sectional Study显示文摘Objective To examine HIV self-testing uptake and its determinates among men who have sex with men(MSM) in Beijing, China. Methods A cross-sectional online survey was conducted in Beijing, China in 2016. Participants were users of a popular Chinese gay networking application and had an unknown or negative HIV status. Univariate and multivariate logistic regression analyses were conducted to examine factors associated with HIV self-testing based on adjusted odds ratio(AOR) and 95% confidence interval(CI). Results Among the 5,996 MSM included in the study, 2,383(39.7%) reported to have used HIV self-testing kits. Willingness to use an HIV self-test kit in the future was expressed by 92% of the participants. High monthly income(AOR = 1.49; CI = 1.10-2.02; P = 0.010), large number of male sex partners(≥ 2: AOR = 1.24; CI = 1.09-1.43; P = 0.002), sexual activity with commercial male sex partners(≥ 2: AOR = 1.94; CI = 1.34-2.82; P = 0.001), long-term drug use(AOR = 1.42; CI = 1.23-1.62; P < 0.001), and long-term HIV voluntary counseling and testing(VCT) attendance(AOR = 3.62; CI = 3.11-4.22; P < 0.001) were all associated with increased odds of HIV self-testing uptake. Conclusion The nearly 40% rate of HIV self-testing uptake among MSM in our sample was high. In addition, an over 90% willingness to use kits in the future was encouraging. HIV self-testing could be an important solution to help China achieve the global target of having 90% of all people living with HIV diagnosed by 2020. | REN Xian Long WU Zun You MI Guo Dong MCGOOGAN Jennifer ROU Ke Ming ZHAO Yan | 2017 | Biomedical and Environmental Sciences2017，30，6： | 13 |
| 10 | 17β-estradiol contributes to the accumulationof myeloid-derived suppressor cells in bloodby promoting TNF-αsecretion显示文摘雌激素被影响有免疫力的房间的发展和激活强烈在有免疫力的回答处于性差别含有。最近的研究显示出那导出 myeloid 的 suppressor 房间(MDSC ) ,源于 CD11b + 在病理学的条件下面的 Gr-1 + myeloid 房间,在 modulating 免疫者回答起重要作用。然而,它仍然是未知的 MDSC 上的雌激素的效果。在现在的学习,我们在调整 MDSC 的累积上调查了雌激素的效果和机制。与有全身的豺狼座 erythematosus (SLE ) 的男病人相比,有 SLE 的女病人在浆液在外部血 mononuclear 房间和肿瘤坏死因素(TNF-) 的高水平显示出 MDSC 的更高的频率,这被发现。尤其是,在有 SLE 的女病人的浆液的 estradiol 水平断然与 MDSC 的频率被相关。而且, 17-estradiol 能支持 TNF -- 在由增加 CD11b + Gr-1 + 房间。而且, 17-estradiol 在 vivo 支持了 TNF- 的分泌物,它贡献了 CD11b + Gr-1 + 房间。另外,雌老鼠显示出 CD11b + Gr-1 + 房间和更高的 TNF- 比匹配年龄的雄的老鼠在血铺平。这些数据显示 17-estradiol 由支持 TNF- 分泌物在血贡献 MDSC 的累积,它增加 CD11b + Gr-1 + 房间。我们的调查结果在发炎和自体免疫的疾病的流行提供新卓见进性差别的机制。 | Guanjun Dong Ming You Hongye Fan Jianjian Ji Liang Ding Pengfei Li Yayi Hou | 2015 | Acta Biochimica et Biophysica Sinica2015，47，8： | 11 |
| 11 | Admission delay is associated with worse surgical outcomes for elderly hip fracture patients:A retrospective observational study显示文摘BACKGROUND:The influence of surgical delay on mortality and morbidity has been studied extensively among elderly hip fracture patients.However,most studies only focus on the timing of surgery when patients have already been hospitalized,without considering pre-admission waiting time.Therefore,the present study aims to explore the infl uence of admission delay on surgical outcomes.METHODS:In this retrospective study,we recorded admission timing and interval from admission to surgery for included patient.Other covariates were also collected to control confounding.The primary outcome was 1-year mortality.The secondary outcomes were 1-month mortality,3-month mortality,ICU admission and postoperative pneumonia.We mainly used multivariate logistic regression to determine the effect of admission timing on postoperative outcomes.An additional survival analysis was also performed to assess the impact of admission delay on survival status in the fi rst year after operation.RESULTS:The proportion of patients hospitalized on day 0,day 1,day 2 after injury was 25.4%,54.7%and 66.3%,respectively.And 12.6%patients visited hospital one week later after injury.Mean time from admission to surgery was 5.2 days(standard deviation 2.8 days).Hospitalization at one week after injury was a risk factor for 1-year mortality(OR 1.762,95%CI 1.026–3.379,P=0.041).CONCLUSION:Admission delay of more than one week is signifi cantly associated with higher 1-year mortality.As a supplement to the current guidelines which emphasizes early surgery after admission,we also advocate early admission once patients get injured. | Wei He Yue-yang You Kai Sun Chen Xie Yue Ming Li-na Yu Feng-jiang Zhang Min Yan | 2020 | World Journal of Emergency Medicine2020，11，1： | 11 |
| 12 | Multi-dimensional scenario forecast for generation of multiple wind farms显示文摘A novel multi-dimensional scenario forecast approach which can capture the dynamic temporal-spatial interdependence relation among the outputs of multiple wind farms is proposed.In the proposed approach,support vector machine(SVM)is applied for the spot forecast of wind power generation.The probability density function(PDF)of the SVM forecast error is predicted by sparse Bayesian learning(SBL),and the spot forecast result is corrected according to the error expectation obtained.The copula function is estimated using a Gaussian copula-based dynamic conditional correlation matrix regression(DCCMR)model to describe the correlation among the errors.And the multidimensional scenario is generated with respect to the estimated marginal distributions and the copula function.Test results on three adjacent wind farms illustrate the effectiveness of the proposed approach. | Ming YANG You LIN Simeng ZHU Xueshan HAN Hongtao WANG | 2015 | Journal of Modern Power Systems and Clean Energy2015，3，3： | 10 |
| 13 | PandaX: a liquid xenon dark matter experiment at CJPL显示文摘PandaX is a large liquid-xenon detector experiment usable for direct dark-matter detection and 136Xe double-beta decay search.The central vessel was designed to accommodate a staged target volume increase from initially 120 kg(stage I)to 0.5 t(stage II)and eventually to a multi-ton scale.The experiment is located in the Jinping Deep-Underground Laboratory in Sichuan,China.The detector operates in dual-phase mode,allowing detection of both prompt scintillation,and ionization charge through proportional scintillation.In this paper a detailed description of the stage I detector design and performance as well as results established during the commissioning phase are presented. | CAO XiGuang CHEN Xun CHEN YunHua CUI XiangYi FANG DeQing FU ChangBo GIBONI Karl L. GONG HaoWei GUO GuoDong HE Ming HU Jie HUANG XingTao JI XiangDong JU YongLin LI ShaoLi LIN Qing LIU HuaXuan LIU JiangLai LIU Xiang LORENZON Wolfgang MA YuGang MAO YaJun NI KaiXuan PUSHKIN Kirill REN XiangXiang SCHUBNELL Michael SHEN ManBing SHI YuJie STEPHENSON Scott TAN AnDi TARLé Greg WANG HongWei WANG JiMing WANG Meng WANG XuMing WANG Zhou WEI YueHuan WU ShiYong XIAO MengJiao XIAO Xiang XIE PengWei YE Tao YOU YingHui ZEN XiongHui ZHANG Hua ZHANG Tao ZHAO HaiYing ZHAO Li ZHOU XiaoPeng ZHU ZhongHua | 2014 | Science China(Physics,Mechanics & Astronomy)2014，57，8： | 9 |
| 14 | Clinical Course and Outcome Patterns of Acute-on-chronic Liver Failure:A Multicenter Retrospective Cohort Study显示文摘Background and Aims:Acute-on-chronic liver failure(ACLF)is acute decompensation of liver function in the setting of chronic liver disease,and characterized by high short-term mortality.In this study,we sought to investigate the clinical course of patients at specific time points,and to propose dynamic prognostic criteria.Methods:We assessed the clinical course of 453 patients with ACLF during a 12-week follow-up period in this retrospective multicenter study.The clinical course of patients was defined as disease recovery,improvement,worsening or steady patterns based on the variation tendency in prothrombin activity(PTA)and total bilirubin(TB)at different time points.Results:Resolution of PTA was observed in 231 patients(51%)at 12 weeks after the diagnosis of ACLF.Among the remaining patients,66(14.6%)showed improvement and 156(34.4%)showed a steady or worsening course.In patients with resolved PTA,the clinical course of TB exhibited resolved pattern in 95.2%,improved in 3.9%,and steady or worse in 0.8%.Correspondingly,in patients with improved PTA,these values for TB were 28.8%,27.3%,and 43.9%,respectively.In patients with steady or worsening PTA,these values for TB were 5.7%,32.3%,and 65.6%,respectively.Dynamic prognostic criteria were developed by combining the clinical course of PTA/TB and the clinical outcomes at 4 and 12 weeks after diagnosis in ACLF patients.Conclusions:We propose the following dynamic prognostic criteria:rapid progression,slow progression,rapid recovery,slow recovery,and slow persistence,which lay the foundation for precise prediction of prognosis and the improvement of ACLF therapy. | Man-Man Xu Ming Kong Peng-Fei Yu Ying-Ying Cao Fang Liu Bing Zhu Yi-Zhi Zhang Wang Lu Huai-Bin Zou Bin-Wei Duan Shao-Li You Shao-Jie Xin Tao Han Zhong-Ping Duan Yu Chen | 2021 | Journal of Clinical and Translational Hepatology2021，9，5： | 8 |
| 15 | Spatial associations of tree species in a subtropical evergreen broad-leaved forest显示文摘Aims The spatial segregation hypothesis and the low-frequency hypothesis are two important proposed mechanisms that delay or prevent competitive exclusion in ecosystems.Because tree species interact with their neighbors,the importance of these potential processes can be investigated by analyzing the spatial structures of tree species.Methods The distribution of the adults of 27 common tree species in a fully mapped 5-ha subtropical forest plot in Baishanzu,eastern China,was analyzed to investigate the community-level intra-and interspecific spatial association patterns.We first tested for the overall spatial pattern in the 5-to 40-m neighborhoods and classified first-order bivariate associations with a diametric scheme based on Ripley’s K and nearest-neighbor statistic(G-function).Then heterogeneous Poisson null models were used to distinguish second-order interactions from overall spatial associations(including first-order effects).Finally,we analyzed correlations between the existence of species interactions and some attributes of the species involved.Important Findings Partial overlap and segregation increased with scale,whereas mixing decreased.Nearly 70%of the species pairs occurred less than expected at random,and only 3.4%of the species pairs were well mixed;11.0%of all species pairs showed significant small-scale interactions,which was a greater frequency than expected by chance if species are abundant or prefer the same habitat,but less frequent than expected if species are highly aggregated.This suggests that both spatial segregation and low frequency of species facilitate species coexistence by reducing the opportunity that trees of two species encounter each other.The study also revealed that positive interactions were more prevalent than negative interactions in the forest,which indicates that positive interactions may have important effects on forest species assemblies. | Zheng R.Luo Ming J.Yu De L.Chen You G.Wu Bing Y.Ding | 2012 | Journal of Plant Ecology2012，5，3： | 8 |
| 16 | Research progress on squeeze casting in China显示文摘Squeeze casting is a technology with short route,high efficiency and precise forming,possessing features of casting and plastic processing.It is widely used to produce high performance metallic structural parts.As energy conservation and environmental protection concerns have risen,lightweight and high performance metal parts are urgently needed,which accelerated the development of squeeze casting technology over the past two decades in China.In this paper,research progress on squeeze casting alloys,typical parts manufacturing and development of squeeze casting equipment in China are introduced.The future trend and development priorities of squeeze casting are discussed. | Li Yuanyuan Zhang Weiwen Zhao Haidong You Dongdong Zhang Datong Shao Ming Zhang Wen | 2014 | China Foundry2014，11，4： | 8 |
| 17 | Recent progress of integrated circuits and optoelectronic chips显示文摘Integrated circuits(ICs)and optoelectronic chips are the foundation stones of the modern information society.The IC industry has been driven by the so-called'Moore's law'in the past 60 years,and now has entered the post Moore's law era.In this paper,we review the recent progress of ICs and optoelectronic chips.The research status,technical challenges and development trend of devices,chips and integrated technologies of typical IC and optoelectronic chips are focused on.The main contents include the development law of IC and optoelectronic chip technology,the IC design and processing technology,emerging memory and chip architecture,brain-like chip structure and its mechanism,heterogeneous integration,quantum chip technology,silicon photonics chip technology,integrated microwave photonic chip,and optoelectronic hybrid integrated chip. | Yue HAO Shuiying XIANG Genquan HAN Jincheng ZHANG Xiaohua MA Zhangming ZHU Xingxing GUO Yahui ZHANG Yanan HAN Ziwei SONG Yan LIU Ling YANG Hong ZHOU Jiangyi SHI Wei ZHANG Min XU Weisheng ZHAO Biao PAN Yangqi HUANG Qi LIU Yimao CAI Jian ZHU Xin OU Tiangui YOU Huaqiang WU Bin GAO Zhiyong ZHANG Guoping GUO Yonghua CHEN Yong LIU Xiangfei CHEN Chunlai XUE Xingjun WANG Lixia ZHAO Xihua ZOU Lianshan YAN Ming LI | 2021 | Science China(Information Sciences)2021，64，10： | 7 |
| 18 | Study of differential polymerase chain reaction of C-erbB-2 oncogene amplification in gastric cancer显示文摘METHODSCerbB2oncogeneamplificationwasexaminedbyusingdiferentialpolymerasechainreaction(dPCR)insurgicalandendoscopicspecimen... | JI Feng, PENG Qing Bi, ZHAN Jing Biao and LI You Ming | 1999 | World Journal of Gastroenterology1999，5，2： | 7 |
| 19 | Two New Cyclic Peptides from Psammosilene tunicoides显示文摘From Psammosilene tunicoides W. C. Wu et C. Y Wu, two cyclic octapeptides (namedpsammosilenins A and B) were isolated. Their structures were determined as cyclo(-Pro1-phe1-Pro2-Phe2-Phe3-Ala-Pro3-Leu-) and cyclo(-Pro1-Gly-Phe1-Val-Pro2-Phe2-Thr-Ile) by spectroscopicmethods. | Zhong Tao DING You Chu WANG Jun ZHOU Ning Hua TAN Hou Ming WU(1Laboratory of Phytochemisty, Kunming institute of Botany, Academia Sinica, Kunming 650204)(2Department of Chemistry, Yunnan University, Kunming 650091)(3State Key Laboratory of Bio-Organic | 1999 | Chinese Chemical Letters1999，10，12： | 6 |
| 20 | Elevated Neurosteroids in the Lateral Thalamus Relieve Neuropathic Pain in Rats with Spared Nerve Injury显示文摘Neurosteroids are synthesized in the nervous system from cholesterol or steroidal precursors imported from peripheral sources. These compounds are important allosteric modulators of c-aminobutyric acid A receptors(GABA_ARs), which play a vital role in pain modulation in the lateral thalamus, a main gate where somatosensory information enters the cerebral cortex. Using high-performance liquid chromatography/tandem mass spectrometry,we found increased levels of neurosteroids(pregnenolone,progesterone, deoxycorticosterone, allopregnanolone, and tetrahydrodeoxycorticosterone) in the chronic stage of neuropathic pain(28 days after spared nerve injury) in rats.The expression of the translocator protein TSPO, the upstream steroidogenesis rate-limiting enzyme, increased at the same time. In vivo stereotaxic microinjection of neurosteroids or the TSPO activator AC-5216 into the lateral thalamus(AP-3.0 mm, ML ±3.0 mm, DV 6.0 mm)alleviated the mechanical allodynia in neuropathic pain,while the TSPO inhibitor PK 11195 exacerbated it. The analgesic effects of AC-5216 and neurosteroids were significantly attenuated by the GABA_AR antagonist bicuculline. These results suggested that elevated neurosteroids in the lateral thalamus play a protective role in the chronic stage of neuropathic pain. | Meng Zhang Jia Liu Meng-Meng Zhou Honghai Wu Yanning Hou Yun-Feng Li Yuxin Yin Lemin Zheng Feng-Yu Liu Ming Yi You Wan | 2016 | Neuroscience Bulletin2016，32，4： | 6 |
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