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| 1 | Daikenchuto (Da-Jian-Zhong-Tang) ameliorates intestinal fibrosis by activating myofibroblast transient receptor potential ankyrin 1 channel显示文摘AIM To investigate the anti-fibrotic effects of the traditional oriental herbal medicine Daikenchuto(DKT) associated with transient receptor potential ankyrin 1(TRPA1) channels in intestinal myofibroblasts. METHODS Inflammatory and fibrotic changes were detected in a2,4,6-trinitrobenzenesulfonic acid(TNBS) chronic colitis model of wild-type and TRPA1-knockout(TRPA1-KO) mice via pathological staining and immunoblotting analysis.Ca^(2+) imaging experiments examined the effects of DKT and its components/ingredients on intestinal myofibroblast(In Myo Fib) cell TRPA1 channel function.Profibrotic factors and transforming growth factor (TGF) -β1-associated signaling were tested in an In Myo Fib cell line by q PCR and immunoblotting experiments.Samples from non-stenotic and stenotic regions of the intestines of patients with Crohn’s disease (CD) were used for pathological analysis. RESULTS Chronic treatment with TNBS caused more severe inflammation and fibrotic changes in TRPA1-KO than in wild-type mice.A one-week enema administration of DKT reduced fibrotic lesions in wild-type but not in TRPA1-KO mice.The active ingredients of DKT,i.e.,hydroxyα-sanshool and 6-shogaol,induced Ca^(2+) influxes in In Myo Fib,and this was antagonized by co-treatment with a selective TRPA1 channel blocker,HC-030031.DKT counteracted TGF-β1-induced expression of TypeⅠcollagen andα-smooth muscle actin (α-SMA) ,which were accompanied by a reduction in the phosphorylation of Smad-2 and p38-mitogen-activated protein kinase (p38-MAPK) and the expression of myocardin.Importantly,24-h incubation with a DKT active component Japanese Pepper increased the m RNA and protein expression levels of TRPA1 in In Myo Fibs,which in turn negatively regulated collagen synthesis.In the stenotic regions of the intestines of CD patients,TRPA1 expression was significantly enhanced.CONCLUSION The effects of DKT on the expression and activation of the TRPA1 channel could be advantageous for suppressing intestinal fibrosis,and benefit inflammatory bowel disease treatment. | Keizo Hiraishi Lin-Hai Kurahara Miho Sumiyoshi Yao-Peng Hu Kaori Koga Miki Onitsuka Daibo Kojima Lixia Yue Hidetoshi Takedatsu Yu-Wen Jian Ryuji Inoue | 2018 | World Journal of Gastroenterology2018，24，35： | 2 |
| 2 | Up-regulation of ADRP in fatty liver in human and liver steatosis in mice fed with high fat diet显示文摘 | Wataru Motomura Mitsutaka Inoue Takaaki Ohtake Nubuhiko Takahashi Miho Nagamine Satoshi Tanno Yutaka Kohgo Toshikatsu Okumura | 2005 | Biochemical and Biophysical Research Communications2005，，4： | 1 |
| 3 | 改良热分解法制备新型钛酸钙涂层的实验研究显示文摘目的研究改良热分解法制备的钛金属表面钛酸钙复合涂层材料的特性。方法应用改良热分解法在钛金属表面制备钛酸钙复合涂层材料,该涂层由含有无定形碳的钛酸钙(CaTiO3-αC)与羟基磷灰石(HA)组成。实验中通过控制钙磷比(Ca/P)、钙钛比(Ca/Ti)、烧结温度和烧结速率,使CaTiO3颗粒的晶体结构中含有摩尔分数为4%的无定形碳。结果通过对HA中碳的检测,证实了存在碳酸磷灰石的结构。钛表面的黏附-分离实验显示,CaTiO3-αC/HA涂层的黏附强度是单纯HA涂层的2.5倍。结论改良热分解法制备的CaTiO3-αC层(0.6μm厚)和含有碳酸磷灰石的HA层(2.4μm厚)组成的双层生物活性材料能够作为钛表面理想的涂层材料。 | Noriyuki Nagai Bao-hong Zhao Mika Okauchi Andrea Rodriguez Mehmet Gunduz Hailong Hu Midori Kubota Noriyuki Nagaoka Miho Inoue Hitoshi Nagatsuka Tohru Takagi Masam Akao | 2010 | 中国实用口腔科杂志2010，3，6： | 1 |
| 4 | Locally Injected Dexmedetomidine Inhibits Carrageenin-Induced Inflammatory Responses in the Injected Region显示文摘 | Shintaro Sukegawa Hitoshi Higuchi Miho Inoue Hitoshi Nagatsuka Shigeru Maeda Takuya Miyawaki | 2014 | Anesthesia & Analgesia2014，，2： | 1 |
| 5 | Structure of the fission yeast S. pombe telomeric Tpz1-Poz1-Rap1 complex显示文摘 | Jing Xue Hongwen Chen Jian Wu Miho Takeuchi Haruna Inoue Yanmei Liu Hong Sun Yong Chen Junko Kanoh Ming Lei | 2017 | Cell Research2017，27，12： | 1 |
| 6 | Induction and Activation of the Transcription Factor NFATc1 (NFAT2) Integrate RANKL Signaling in Terminal Differentiation of Osteoclasts显示文摘 | Hiroshi Takayanagi Sunhwa Kim Takako Koga Hiroshi Nishina Masashi Isshiki Hiroki Yoshida Akio Saiura Miho Isobe Taeko Yokochi Jun-ichiro Inoue Erwin F. Wagner Tak W. Mak Tatsuhiko Kodama Tadatsugu Taniguchi | 2002 | Developmental Cell2002，，6： | 1 |
| 7 | Effects of scallop shell extract on scopolamine-induced memory impairment and MK801-induced locomotor activity显示文摘Objective:To evaluate the neuroprotective effects of the organic components of scallop shells(scallop shell extract) on memory impairment and locomotor activity induced by scopolamine or 5-methyl-10,11-dihydro-5H-dibenzo(a,d) cyclohepten-5,10-imine(MK801).Methods:Effect of the scallop shell extract on memory impairment and locomotor activity was investigated using the Y-maze test,the Morris water maze test,and the open field test.Results:Scallop shell extract significantly reduced scopolamine-induced short-term memory impairment and partially reduced scopolamine-induced spatial memory impairment in the Morris water maze test.Scallop shell extract suppressed scopolamine-induced elevation of acetylcholine esterase activity in the cerebral cortex.Treatment with scallop shell extract reversed the increase in locomotor activity induced by scopolamine.Scallop shell extract also suppressed the increase in locomotor activity induced by MK801.Conclusions:Our results provide initial evidence that scallop shell extract reduces scopolamine-induced memory impairment and suppresses MK-801-induced hyperlocomotion. | Yasushi Hasegawa Tatsuro Inoue Satoshi Kawaminami Miho Fujita | 2016 | Asian Pacific Journal of Tropical Medicine2016，9，7： | 1 |
| 8 | The Mitogenic Activity of Hepatocyte Growth Factor on Rat Hepatocytes Is Dependent upon Endogenous Transforming Growth Factor-α显示文摘 | Tomoaki Tomiya Itsuro Ogata Miho Yamaoka Mikio Yanase Yukiko Inoue Kenji Fujiwara | 2000 | The American Journal of Pathology2000，，5： | 1 |
| 9 | Locally Injected Dexmedetomidine Inhibits Carrageenin-Induced Inflammatory Respon- ses in the Injected Region显示文摘 | Shintaro Sukegawa Hitoshi I-Iiguehi Miho Inoue | 2014 | anesthesia & analgesia2014，118，2： | 1 |
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