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| 1 | Animal models of acute gastric mucosal injury: Macroscopic and microscopic evaluation显示文摘Acute gastric mucosal injury is a common disorder of the gastrointestinal tract and the search for new therapeutics is ongoing. The aim of this study is to update and expand the information related to the most widely used rat models of acute gastric ulcer, the ethanol‐induced ulcer and the indomethacin‐induced ulcer. These two models are compared in terms of macroscopic and microscopic features. Experimentally, ethanol was given orally in a single dose and indomethacin was subcutaneously injected into male Wistar rats. After ulcerative challenges, the stomachs were removed and visually inspected. Anti‐ulcerative drugs were used to validate the models. Histological analysis of the stomachs determined the microscopic score. The methodology used for model evaluation applied to macroscopic and microscopic gastric lesions. With these methods it was possible to induce lesions in the gastric mucosa. Microscopic evaluation permitted assessment of the inflammatory and apoptotic impact in the mucosa not observable by macroscopic evaluation. Groups of animals were treated with two standard drugs: sulcralfate suspension or lansoprazole solution. Both drugs reduced macroscopic and microscopic lesions, particularly the hemorrhagic ones. Both models induced acute gastric mucosal injury and no single evaluation method can address all the aspects of the pathology of gastric lesions. As a complement to macroscopic evaluation, microscopy appears to be a relevant tool to selectively identify specific aspects of the development of mucosal injury, quantify the extent of lesions, and contribute to an appropriate interpretation of results. The score systems established here offer a reliable method for testing antiulcer drugs. | Sandra Simoes Rui Lopes Maria Celeste Dias Campos Maria Joao Marruz Maria Eugenia Meirinhos da Cruz Luisa Corvo | 2019 | Animal Models and Experimental Medicine2019，2，2： | 7 |
| 2 | Liquid chromatographic methods for determination of the new antiepileptic drugs stiripentol, retigabine, rufinamide and perampanel: A comprehensive and critical review显示文摘The new antiepileptic drugs perampanel,retigabine,rufinamide and stiripentol have been recently approved for different epilepsy types.Being them an innovation in the antiepileptics armamentarium,a lot of investigations regarding their pharmacological properties are yet to be performed.Besides,considering their broad anticonvulsant activities,an extension of their therapeutic indications may be worthy of investigation,especially regarding other seizure types as well as other central nervous system disorders.Although different liquid chromatographic(LC)methods coupled with ultraviolet,fluorescence,mass or tandem-mass spectrometry detection have already been developed for the determination of perampanel,retigabine,rufinamide and stiripentol,new and more cost-effective methods are yet required.Therefore,this review summarizes the main analytical aspects regarding the liquid chromatographic methods developed for the analysis of perampanel,retigabine(and its main active metabolite),rufinamide and stiripentol in biological samples and pharmaceutical dosage forms.Furthermore,the physicochemical and stability properties of the target compounds will also be addressed.Thus,this review gathers,for the first time,important background information on LC methods that have been developed and applied for the determination of perampanel,retigabine,rufinamide and stiripentol,which should be considered as a starting point if new(bio)analytical techniques are aimed to be implemented for these drugs. | Sara Meirinho Marcio Rodrigues Ana Fortuna Amílcar Falcao Gilberto Alves | 2021 | Journal of Pharmaceutical Analysis2021，11，4： | 2 |
| 3 | RAPD and ISSR molecular markers in Olea europaea L.: Genetic variability and molecular cultivar identification显示文摘 | Paula Martins-Lopes José Lima-Brito Sónia Gomes Julieta Meirinhos Luís Santos Henrique Guedes-Pinto | 2007 | Genetic Resources and Crop Evolution2007，，1： | 1 |
| 4 | Repeat Around: A software tool for finding and visualizing repeats in circular genomes and its application to a human mtDNA database 显示文摘 | Goios A Meirinhos J Rocha R | 2006 | Mitoehondrion2006，6，4： | 1 |
| 5 | Heme oxygenase-1 protects retinal endothe- lial cells against high glucose-and oxidative/nitrosative stress- induced toxicity显示文摘 | Castilho/k Aveleira CA Leal EC SimSes NF Fernandes CR Meirinhos RI | 2012 | PLoS One2012，7，42： | 1 |
| 6 | RAPD and ISSR molecular markers in Olea europaea L.: Genetic variability and molecular cultivar identification显示文摘 | Paula Martins-Lopes José Lima-Brito Sónia Gomes Julieta Meirinhos Luís Santos Henrique Guedes-Pinto | 2007 | Genetic Resources and Crop Evolution2007，，1： | 1 |
| 7 | Analysis and quantification of flavonoidic compounds from Portuguese olive (Olea Europaea L) leaf cultivars显示文摘 | Meirinhos Julieta Silva | 2005 | Natural product research2005，19，2： | 1 |
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