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| 1 | The impact of synthetic biology on drug discovery显示文摘 | Wilfried Weber Martin Fussenegger | 2009 | Drug Discovery Today2009，，19： | 1 |
| 2 | Adult Stem Cell Therapy of Female Stress Urinary Incontinence显示文摘 | Michael Mitterberger Germar-Michael Pinggera Rainer Marksteiner Eva Margreiter Martin Fussenegger Ferdinand Frauscher Hanno Ulmer Steffen Hering Georg Bartsch Hannes Strasser | 2007 | European Urology2007，，1： | 1 |
| 3 | Molecular Regulation of Cellcycle Progression and Apoptosis in Mammalian Cells, Implications for Biotechnology 显示文摘 | FUSSENEGGER MARTIN BAILEY JAMES | 1998 | Biotechnology Progress1998，14，6： | 1 |
| 4 | Myoblast and Fibroblast Therapy for Post-Prostatectomy Urinary Incontinence: 1-Year Followup of 63 Patients显示文摘 | Michael Mitterberger Rainer Marksteiner Eva Margreiter Germar M. Pinggera Ferdinand Frauscher Hanno Ulmer Martin Fussenegger Georg Bartsch Hannes Strasser | 2008 | The Journal of Urology2008，，1： | 1 |
| 5 | Adult Stem Cell Therapy of Female Stress Urinary Incontinence显示文摘 | Michael Mitterberger Germar-Michael Pinggera Rainer Marksteiner Eva Margreiter Martin Fussenegger Ferdinand Frauscher Hanno Ulmer Steffen Hering Georg Bartsch Hannes Strasser | 2007 | European Urology2007，，1： | 1 |
| 6 | Therapeutic cell engineering:designing programmable synthetic genetic circuits in mammalian cells显示文摘Cell therapy approaches that employ engineered mam-malian cells for on-demand production of therapeutic agents in the patient's body are moving beyond proof-of-concept in translational medicine.The therapeutic cells can be customized to sense user-defined signals,pro-cess them,and respond in a programmable and pre-dictable way.In this paper,we introduce the available tools and strategies employed to design therapeutic cells.Then,various approaches to control cell behav-iors,including open-loop and closed-loop systems,are discussed.We also highlight therapeutic applications of engineered cells for early diagnosis and treatment of various diseases in the clinic and in experimental dis-ease models.Finally,we consider emerging technolo-gies such as digital devices and their potential for incorporation into future cell-based therapies. | Maysam Mansouri Martin Fussenegger | 2022 | Protein & Cell2022，13，7： | 0 |
| 7 | Engineered poly(A)-surrogates for translational regulation and therapeutic biocomputation in mammalian cells显示文摘Here,we present a gene regulation strategy enabling programmable control over eukaryotic translational initiation.By excising the natural poly-adenylation(poly-A)signal of target genes and replacing it with a synthetic control region harboring RNA-binding protein(RBP)-specific aptamers,cap-dependent translation is rendered exclusively dependent on synthetic translation initiation factors(STIFs)containing different RBPs engineered to conditionally associate with different eIF4F-binding proteins(eIFBPs).This modular design framework facilitates the engineering of various gene switches and intracellular sensors responding to many user-defined trigger signals of interest,demonstrating tightly controlled,rapid and reversible regulation of transgene expression in mammalian cells as well as compatibility with various clinically applicable delivery routes of in vivo gene therapy.Therapeutic efficacy was demonstrated in two animal models.To exemplify disease treatments that require on-demand drug secretion,we show that a custom-designed gene switch triggered by the FDA-approved drug grazoprevir can effectively control insulin expression and restore glucose homeostasis in diabetic mice.For diseases that require instantaneous sense-and-response treatment programs,we create highly specific sensors for various subcellularly(mis)localized protein markers(such as cancer-related fusion proteins)and show that translation-based protein sensors can be used either alone or in combination with other cell-state classification strategies to create therapeutic biocomputers driving self-sufficient elimination of tumor cells in mice.This design strategy demonstrates unprecedented flexibility for translational regulation and could form the basis for a novel class of programmable gene therapies in vivo. | Jiawei Shao Shichao Li Xinyuan Qiu Jian Jiang Lihang Zhang Pengli Wang Yaqing Si Yuhang Wu Minghui He Qiqi Xiong Liuqi Zhao Yilin Li Yuxuan Fan Mirta Viviani Yu Fu Chaohua Wu Ting Gao Lingyun Zhu Martin Fussenegger Hui Wang Mingqi Xie | 2024 | Cell Research2024，34，1： | 0 |
| 8 | Direct electrification of silicon microfluidics for electric field applications显示文摘Microfluidic systems are widely used in fundamental research and industrial applications due to their unique behavior, enhanced control, and manipulation opportunities of liquids in constrained geometries. In micrometer-sized channels, electric fields are efficient mechanisms for manipulating liquids, leading to deflection, injection, poration or electrochemical modification of cells and droplets. While PDMS-based microfluidic devices are used due to their inexpensive fabrication, they are limited in terms of electrode integration. Using silicon as the channel material, microfabrication techniques can be used to create nearby electrodes. Despite the advantages that silicon provides, its opacity has prevented its usage in most important microfluidic applications that need optical access. To overcome this barrier, silicon-on-insulator technology in microfluidics is introduced to create optical viewports and channel-interfacing electrodes. More specifically, the microfluidic channel walls are directly electrified via selective, nanoscale etching to introduce insulation segments inside the silicon device layer, thereby achieving the most homogeneous electric field distributions and lowest operation voltages feasible across microfluidic channels. These ideal electrostatic conditions enable a drastic energy reduction, as effectively shown via picoinjection and fluorescence-activated droplet sorting applications at voltages below 6 and 15 V, respectively, facilitating low-voltage electric field applications in next-generation microfluidics. | Diego Monserrat Lopez Philipp Rottmann Gabriel Puebla-Hellmann Ute Drechsler Marcel Mayor Sven Panke Martin Fussenegger Emanuel Lörtscher | 2023 | Microsystems & Nanoengineering2023，9，3： | 0 |
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