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| 1 | Antineoplastic effects of deoxyelephantopin,a sesquiterpene lactone from Elephantopusscaber, on lung adenocarcinoma (A549) cells显示文摘OBJECTIVE: Deoxyelephantopin, a sesquiterpene lactone from Elephantopus scaber, showed inhibition of the growth of various tumor cells in vitro. In the present study, we investigated the cytotoxicity and apoptosis-inducing capacity of deoxyelephantopin on lung adenocarcinoma (A549) cells. METHODS: The cytotoxic effect of deoxyelephantopin on A549 cells and normal lymphocytes was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and 50% inhibitory concentration (IC50) value was determined. The self-renewal and proliferating potential of A549 cells after treatment with deoxyelephantopin were examined by colony formation assay. Cellular morphology of deoxyelephantopin-treated cells was observed using phasecontrast microscopy. The induction of apoptosis was evaluated using acridine orange and ethidium bromide staining, Hoechst 33342 staining, terminal deoxynucleotidyl transferase-mediated dUTP biotin nick end-labeling (TUNEL) assay, DNA fragmentation analysis and Annexin V-fluorescein isothiocyanate staining by flow cytometry. Activation of caspases was detected using fluorogenic substrate specific to caspases 2, 3, 8 and 9 and flow cytometric analysis. The total cellular DNA content and expression of cleaved poly (ADP-ribose) polymerase was also analyzed. RESULTS: Deoxyelephantopin exhibited cytotoxicity to A549 cells (IC50=12.287 μg/mL), however, there was no toxicity towards normal human lymphocytes. Deoxyelephantopin suppressed the colony-forming ability of A549 cells in a dose-dependent manner. Acridine orange, ethidium bromide and Hoechst 33342 staining showed cell shrinkage, chromosomal condensation and nuclear fragmentation, indicating induction of apoptosis. Deoxyelephantopin increased apoptosis of A549 cells, as evidenced by more TUNEL-positive cells. DNA fragmentation and Annexin V staining revealed late-stage apoptotic cell population. Deoxyelephantopin inhibited A549 cell growth by cell cycle arrest at G2/M phase and induced apoptosis through both extrinsic and intrinsic pathways. CONCLUSION: These results suggest that deoxyelephantopin has great potential as a new chemotherapeutic agent to be developed further for the treatment of lung cancer. | Farha A. Kabeer Geetha B. Sreedevi Mangalam S. Nair Dhanya S. Rajalekshmi LathaP. Gopalakrishnan Sujathan Kunjuraman Remani Prathapan | 2013 | Journal of Integrative Medicine2013，11，4： | 4 |
| 2 | Design of area and power efficient Radix-4 DIT FFT butterfly unit using floating point fused arithmetic显示文摘In this work, power efficient butterfly unit based FFT architecture is presented. The butterfly unit is designed using floating-point fused arithmetic units. The fused arithmetic units include two-term dot product unit and add-subtract unit. In these arithmetic units, operations are performed over complex data values. A modified fused floating-point two-term dot product and an enhanced model for the Radix-4 FFT butterfly unit are proposed. The modified fused two-term dot product is designed using Radix-16 booth multiplier. Radix-16 booth multiplier will reduce the switching activities compared to Radix-8 booth multiplier in existing system and also will reduce the area required. The proposed architecture is implemented efficiently for Radix-4 decimation in time(DIT) FFT butterfly with the two floating-point fused arithmetic units. The proposed enhanced architecture is synthesized, implemented, placed and routed on a FPGA device using Xilinx ISE tool. It is observed that the Radix-4 DIT fused floating-point FFT butterfly requires 50.17% less space and 12.16% reduced power compared to the existing methods and the proposed enhanced model requires 49.82% less space on the FPGA device compared to the proposed design. Also, reduced power consumption is addressed by utilizing the reusability technique, which results in 11.42% of power reduction of the enhanced model compared to the proposed design. | Prabhu E Mangalam H Karthick S | 2016 | Journal of Central South University2016，23，7： | 2 |
| 3 | Primer premier: Program for design of degenerate primers from a protein sequence 显示文摘 | Singh VK Mangalam AK Dwivedi S | 1998 | Biotechniques1998，24，2： | 1 |
| 4 | Resistive switching in polymethyl methacrylate thin films 显示文摘 | MANGALAM J AGARWAL S RESMI A N | 2016 | Organic Electronics2016，29，： | 1 |
| 5 | On computation of NPMLE for Middle-censored data显示文摘 | Vasudevan Mangalam Gopalan M.Nair Yun zhao | | 0，，： | 1 |
| 6 | On computation of NPMLE for Middle-censored data显示文摘 | Vasudevan Mangalam Gopalan M Nair Yun zhao | 2008 | Statistics and Probability Letters2008，78，： | 1 |
| 7 | Evaluation of api- cally extruded debris and irrigant using three instrumentation techniques 显示文摘 | Mangalam S Rao CV Lakshminarayanan L | 2002 | Endodont2002，14，： | 1 |
| 8 | Cellulose/DNA hybrid nanomaterials显示文摘 | Anand P Mangalam John Simonsen Albert S Benight | 2009 | Biomacromolecules2009，10，： | 1 |
| 9 | HLA class 1I transgenic mice mimic human inflammatory diseases显示文摘 | Mangalam AK Rajagopalan G Taneja V | 2008 | Ady Immunol2008，97，： | 1 |
| 10 | Experimental observation on the relationship between stagnation region,flow oscillations and eddy shedding for circular cylinder显示文摘 | MANGALAM S M KUBENDRAM L R | 1990 | Stability and Transition1990，1，： | 1 |
| 11 | Primer Premier: program for design of degenerate primers from a protein sequence 显示文摘 | Singh V K Mangalam A K Dwivedi S | 1998 | Bio Techniques1998，24，： | 1 |
| 12 | Model owsite emergency plan case study: Toxic gas release from an ammonia storage terminal显示文摘 | Boppana V Ramabrahmam Sreenivasulu B Mangalam M Mallikarjunan | 1996 | J of Loss Prevention in the Process Industries1996，9，： | 1 |
| 13 | A pitui- tary POU-domain protein, Pit-1, activates both growth hor- mone and prolactin promoters transcriptionally 显示文摘 | Mangalam H J Albert V R Ingraham H A | 1989 | Genes Dev1989，3，： | 1 |
| 14 | Primer premier:program for design of degenerate primers from a protein sequence显示文摘 | SINGH V K MANGALAM A DWIVEDI S | 1998 | Biotechniques1998，24，2： | 1 |
| 15 | Effect of acoustic excitation on the flow over a low-Re airfoil显示文摘 | Zaman K B M Q Bar-Sever A Mangalam S M | | 0，，： | 1 |
| 16 | A pituitary POU-domain protein,Pit-1,activates both growth hormone and prolactin promoters transcriptionally显示文摘 | Mangalam H J Albert V R Ingraham H A | 1989 | Genes Dev1989，，3： | 1 |
| 17 | Dielectric prop- erties,thennal decomposition and related aspects of BiA103显示文摘 | MANGALAM R V K BHAT S V IYO A | 2008 | Solid State Commun2008，146，1112： | 1 |
| 18 | HLA class II transgenic mice mimic human inflammatory diseases 显示文摘 | Mangalam AK Rajagopalan G Taneja V | 2008 | Adv Immunol2008，97，1： | 1 |
| 19 | A Pituitary POU domain protein, PIT1, activates both growth hormone and prolactin promoters transcription-ally 显示文摘 | Mangalam H J Albert V A Ingraham H A | 1989 | Genes&Dev1989，3，： | 1 |
| 20 | Primer premier program for design of degenerate primers from a protein sequence显示文摘 | SINGH V K MANGALAM AK DWIVEDI S | 1998 | BioTechniques1998，24，2： | 1 |
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