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| 1 | 全日制放牧BMY牛的活体采卵-体外胚胎生产-胚胎移植研究显示文摘为建立BMY牛的活体采卵(OPU)-体外胚胎生产(IVP)-胚胎移植(ET)快速扩繁技术体系,从人工草地全放牧牛群中选择健康的经产空怀BMY牛作为供体(n=14)集中OPU一次;卵母细胞经TCM-199体外成熟培养(IVM)、Fert-TALP液体外受精(IVF),受精卵用合成输卵管液(SOF)体外培养(IVC)生产胚胎;BMY牛受体(n=81)用CUE-MATETM孕酮阴道栓+EB+FSH+PG方法同期发情处理,对黄体合格的移植OPU-IVP来源鲜胚。研究结果表明:①OPU头均获卵母细胞23.50枚,获10枚以上卵母细胞的供体占85.7%;IVP的平均囊胚数和可用胚数分别为5.21和4.00枚;OPU前8~9d卵巢上有黄体(CL)的供体,头均获卵母细胞数及IVP的囊胚数、可移植胚数、囊胚率和可移植胚率分别比对照组高7.28枚(P<0.05)、7.29枚(P<0.05)、6.00枚(P<0.05)、27.3%(P<0.01)和23.2%(P<0.01);②受体黄体合格率69.1%(56/81),胚胎移植妊娠率37.5%(21/56),产犊率35.7%(20/56);③受体在胚胎移植时注射GnRH,其妊娠率比对照组高16.2%(P>0.05)。结果显示,BMY母牛具有良好的OPU潜力,但个体间差异较大;可通过严格供体选择及改善IVP技术等措施,实现OPU-IVP效率最大化。CUE-MATETM+EB+FSH+PG同期发情处理方法适于在牛规模化胚胎移植(ET)和人工授精(AI)中应用。综合应用OPU、IVP、同期发情和ET技术是加快优良BMY牛扩繁的重要途径之一。 | 和占星 王喆 张继才 王安奎 金显栋 袁希平 Joaquim M.Garcia Marina R.de Lima 何永珍 黄必志 | 2013 | 畜牧兽医学报2013，44，1： | 4 |
| 2 | Medicinal properties of Angelica archangelica root extract: Cytotoxicity in breast cancer cells and its protective effects against in vivo tumor development显示文摘Objective: Although Angelica archangelica is a medicinal and aromatic plant with a long history of use for both medicinal and food purposes, there are no studies regarding the antineoplastic activity of its root.This study aimed to evaluate the cytotoxicity and antitumor effects of the crude extract of A. archangelica root(CEAA) on breast cancer.Methods: The cytotoxicity of CEAA against breast adenocarcinoma cells(4T1 and MCF-7) was evaluated by a 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide(MTT) assay. Morphological and biochemical changes were detected by Hoechst 33342/propidium iodide(PI) and annexin V/PI staining. Cytosolic calcium mobilization was evaluated in cells staining with FURA-4 NW. Immunoblotting was used to determine the effect of CEAA on anti-and pro-apoptotic proteins(Bcl-2 and Bax, respectively).The 4 T1 cell-challenged mice were used for in vivo assay.Results: Using ultra-high-performance liquid chromatography–mass spectrometry analysis, angelicin, a constituent of the roots and leaves of A. archangelica, was found to be the major constituent of the CEAA evaluated in this study(73 mg/mL). The CEAA was cytotoxic for both breast cancer cell lines studied but not for human fibroblasts. Treatment of 4 T1 cells with the CEAA increased Bax protein levels accompanied by decreased Bcl-2 expression, in the presence of cleaved caspase-3 and cytosolic calcium mobilization, suggesting mitochondrial involvement in breast cancer cell death induced by the CEAA in this cell line. No changes on the Bcl-2/Bax ratio were observed in CEAA-treated MCF7 cells. Gavage administration of the CEAA(500 mg/kg) to 4T1 cell-challenged mice significantly decreased tumor growth when compared with untreated animals.Conclusion: Altogether, our data show the antitumor potential of the CEAA against breast cancer cells in vitro and in vivo. Further research is necessary to better elucidate the pharmacological application of the CEAA in breast cancer therapy. | Carlos R.Oliveira Daniel G.Spindola Daniel M.Garcia Adolfo Erustes Alexandre Bechara Caroline Palmeira-dos-Santos Soraya S.Smaili Gustavo J.S.Pereira André Hinsberger Ezequiel P.Viriato Maria Cristina Marcucci Alexandra C.H.F.Sawaya Samantha L.Tomaz Elaine G.Rodrigues Claudia Bincoletto | 2019 | Journal of Integrative Medicine2019，17，2： | 2 |
| 3 | Iachemic hepatitis secondary to the spontaneous rupture of a hepatocellular Carcinoma in a patient with cirrhosis 显示文摘 | Castiella A,Cancio M.Garcia M, | 1996 | Liver1996，16，2： | 1 |
| 4 | Effects of TNF receptor blockade on in vitro cell survival and response to negative energy balance in dairy cattle显示文摘Background: Associative data and some controlled studies suggest that the inflammatory cytokine tumor necrosis factor(TNF) α can induce fatty liver in dairy cattle. However, research demonstrating that TNFα is a necessary component in the etiology of bovine fatty liver is lacking. The aim of this work was to evaluate whether blocking TNFα signaling with a synthetic cyclic peptide(TNF receptor loop peptide; TRLP) would improve liver metabolic function and reduce triglyceride accumulation during feed restriction.Results: Capability of TRLP to inhibit TNFα signaling was confirmed on primary bovine hepatocytes treated with recombinant bovine TNFα and 4 doses of TRLP(0, 1, 10, 50 μmol/L) over 24 h. Next, 4 lactating Holstein cows(parity 1.4 ± 0.5, 433 ± 131 d in milk) in an incomplete Latin rectangle design(3 × 2) were subcutaneously administered with different TRLP doses(0, 1.5, 3.0 mg/kg BW) every 4 h for 24 h, followed by an intravenous injection of TNFα(5 μg/kg BW). Before and for 2 h after TNFα injection, TRLP decreased plasma non-esterified fatty acid(NEFA)concentration(P ≤ 0.05), suggesting an altered metabolic response to inflammation. Finally, 10 non-pregnant, nonlactating Holstein cows(3.9 ± 1.1 yr of age) were randomly assigned to treatments: control(carrier: 57% DMSO in PBS)or TRLP(1.75 mg TRLP/kg BW per day). Treatments were administrated every 4 h for 7 d by subcutaneous injection to feed-restricted cows fed 30% of maintenance energy requirements. Daily blood samples were analyzed for glucose,insulin, β-hydroxybutyrate, NEFA, and haptoglobin concentrations, with no treatment effects detected. On d 7, cows completed a glucose tolerance test(GTT) by i.v. administration of a dextrose bolus(300 mg glucose/kg BW). Glucose,insulin, and NEFA responses failed to demonstrate any significant effect of treatment during the GTT. However, plasma and liver analyses were not indicative of dramatic lipolysis or hepatic lipidosis, suggesting that the feed restriction protocol failed to induce the metabolic state of interest. Injection site inflammation, assessed by a scorer blinded to treatment, was enhanced by TRLP compared to control.Conclusions: Although the TRLP inhibited bovine TNFα signaling and altered responses to i.v. administration of TNFα,repeated use over 7 d caused apparent local allergic responses and it failed to alter metabolism during a feed restriction-induced negative energy balance. Although responses to feed restriction seemed atypical in this study, side effects of TRLP argue against its future use as a tool for investigating the role of inflammation in metabolic impacts of negative energy balance. | C.A.Martel L.K.Mamedova J.E.Minton M.Garcia C.Legallet B.J.Bradford | 2018 | Journal of Animal Science and Biotechnology2018，9，2： | 1 |
| 5 | Analysis of exosome release and its prognostic value in human colorectal cancer显示文摘 | J.Silva V.Garcia M.Rodriguez M.Compte E.Cisneros P.Veguillas J. M.Garcia G.Dominguez Y.Campos‐Martin J.Cuevas C.Pe?a M.Herrera R.Diaz N.Mohammed F.Bonilla | 2012 | Genes Chromosom Cancer2012，，4： | 1 |
| 6 | 预制混凝土节段拼装式轻轨桥创新方法显示文摘1991年,在墨西哥新莱昂州蒙特雷大都市区蒙特雷第一条地铁,长18.5km的1号线竣工并向公众开放。这个预制混凝土分段梁结构由Jean Muller设计,此结构的简支梁标准跨长27m,最大跨长36m。箱梁宽7.40m,高度2.13m。 | 李俊平 Juan José Golli Baamoude Antonio M.Garcia Belliteg | 2010 | 建筑技术开发2010，，4： | 0 |
| 7 | 菲律宾丘陵地土壤保持显示文摘 | Richard T.Yao Jose Nestor M.Garcia | 2002 | 中国水土保持2002，，7： | 0 |
| 8 | IMPLANT OF EX-PRESS MINI GLAUCOMA SHUNT. SURGICAL TECHNIQUE AND RE-SULTS显示文摘Purpose: To determine the effectiveness of the Ex-Press mini glaucoma shunt. Methods: We implanted the new device for treatment of glaucoma in ten patients, following the standard surgical procedure. Results: After a three months follow-up, two patients mantained safe intraocular pressure range without complications. The rest showed a high variability of pressure and a diversity of complications such as fibrosis and | M.L.Hernandez D.Salom F.Morais A.Perez M.Garcia | 2003 | 国际眼科杂志2003，3，1： | 0 |
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