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| 1 | Cryopreservation for delayed circulating tumor cell isolation is a valid strategy for prognostic association of circulating tumor cells in gastroesophageal cancer显示文摘AIM To demonstrate the feasibility of cryopreservation of peripheral blood mononuclear cells(PBMCs) for prognostic circulating tumor cell(CTC) detection in gastroesophageal cancer.METHODS Using 7.5 m L blood samples collected in EDTA tubes from patients with gastroesopheagal adenocarcinoma, CTCs were isolated by epithelial cell adhesion molecule based immunomagnetic capture using the Iso Flux platform. Paired specimens taken during the same blood draw(n = 15) were used to compare number of CTCs isolated from fresh and cryopreserved PBMCs. Blood samples were processed within 24 h to recover the PBMC fraction, with PBMCs used for fresh analysis immediately processed for CTC isolation. Cryopreservation of PBMCs lasted from 2 wk to 25.2 mo(median 14.6 mo). CTCs isolated from pre-treatment cryopreserved PBMCs(n = 43) were examined for associations with clinicopathological variables and survival outcomes.RESULTS While there was a significant trend to a decrease in CTC numbers associated with cryopreserved specimens(mean number of CTCs 34.4 vs 51.5, P = 0.04), this was predominately in samples with a total CTC count of > 50, with low CTC count samples less affected(P = 0.06). There was no significant association between the duration of cryopreservation and number of CTCs. In cryopreserved PBMCs from patient samples prior to treatment, a high CTC count(> 17) was associated with poorer overall survival(OS)(n = 43, HR = 4.4, 95%CI: 1.7-11.7, P = 0.0013). In multivariate analysis, after controlling for sex, age, stage, ECOG performance status, and primary tumor location, a high CTC count remained significantly associated with a poorer OS(HR = 3.7, 95%CI: 1.2-12.4, P = 0.03). CONCLUSION PBMC cryopreservation for delayed CTC isolation is a valid strategy to assist with sample collection, transporting and processing. | Daniel Brungs David Lynch Alison WS Luk Elahe Minaei Marie Ranson Morteza Aghmesheh Kara L Vine Martin Carolan Mouhannad Jaber Paul de Souza Therese M Becker | 2018 | World Journal of Gastroenterology2018，24，7： | 5 |
| 2 | Human capital investments and productivity显示文摘 | Black S Lynch L M | 1996 | American Economic Review1996，86，2： | 1 |
| 3 | Interactions between Fusarium culmorum and its potential biocontrol agent, Trichoderma harzianum, in a packed-bed, continuous-flow column reactor显示文摘 | Cheetham J L Bazin M J Lynch J M | 1997 | Enzyme and Microbial Technology1997，21，5： | 1 |
| 4 | Biotransformation of sevoflurane in dogs and rats 显示文摘 | Lynch S Napoli M D | 1981 | Anesth Analg1981，60，： | 1 |
| 5 | Social and Economic Exchange:Construct Development and Validation 显示文摘 | Shore L M Tetrick L E Lynch P Barksdale K | 2006 | Journal of Applied Social Psychology2006，36，4： | 1 |
| 6 | Nursing Students'Experiences With and Strategic Approaches to Case-based Instruction:a Replication and Comparison Study Between Two Disciplines显示文摘 | Demarco R Hayward L Lynch M | 2002 | The Journal of Nursing Education2002，41，4： | 1 |
| 7 | Dry powder precursors of cubic liquid crystalline nanoparticles(cubosomes)显示文摘 | Spieer P T Small W B II Lynch M L Burns J L | 2002 | J Nanopart Res2002，4，： | 1 |
| 8 | The growth inhibitory role and potential clinical value of macrophage inflammatory protein 1 alpha in myeloid leukemias显示文摘 | Owen LYNCH P J Czaplewski L G Hunter M G | 1998 | Leuk Lymphoma1998，30，2： | 1 |
| 9 | A new terpyrrolic analogue of dipyrrolylquinoxalines: an efficient optical- based sensor for anions in organic media显示文摘 | Sjevcjuk S V Lynch V M Sessler J L | 2004 | Tetrahedron2004，60，11： | 1 |
| 10 | Tomato Ve disease resistance genes encode cell surface-like receptors 显示文摘 | Kawchuk L M Hachey J Lynch D R | 2001 | Proceedings of the National Academy of Sciences2001，95，11： | 1 |
| 11 | Enhanced loading of water-soluble actives into bicontinous cubic phase liquid crystals using cationic surfactant显示文摘 | Lynch M L Boateng A O Hippe A | 2003 | J Colloid Interface Sci2003，260，： | 1 |
| 12 | 查看详情显示文摘 | Zhu L Shabbir S H Gray M Lynch V M Sorey S Anslyn E V | | 0，，： | 1 |
| 13 | The use of colony development for the characterization of bacterial communities in soil and on roots 显示文摘 | Faam D L Whipps J M Lynch J M | 1993 | Microbial Ecology1993，27，： | 1 |
| 14 | Cells with haematopoieric stem cell phenotype in adult human endometrium:relevance to infertility? 显示文摘 | Lynch L Golden-Mason L Eogan M | 2007 | Hum Reprod2007，22，4： | 1 |
| 15 | The influence of cold acclimation on lipid composition and cryobehaviour of the plasma membrane of isolated rye protoplasts 显示文摘 | Steponkus P L Lynch D V Uemura M | 1990 | Philosophical Transactions of the Royal Society B1990，326，： | 1 |
| 16 | Integration of are- sorbable membrane and a bone graft material显示文摘 | Kay SA Wisher Lynch L Marxer M | 1997 | Practical Peri- odontics and Aesthetic Dentistry1997，9，： | 1 |
| 17 | Effect of introduced Pseudomonas fluorescens strains on soil nematode and protozoan populations in the rhizosphere of wheat and pea显示文摘 | Brimecombe M J De L F Lynch J M | 1999 | Microbial ecology1999，38，4： | 1 |
| 18 | Effect of preservatives on the accuracy of mid-infrared milk component testing1 显示文摘 | BARBANO D M WOJCIECHOWSKI K L LYNCH J M | 2010 | Journal of Dairy Science2010，93，12： | 1 |
| 19 | Burial diagenesis of illite/smectite in shales and the origins of authigenic quartz and secondary porosity in sandstones显示文摘 | Lynch F L Lawrence E M Lynton S L | 1997 | Geochimica et Cosmochimica Acta1997，61，10： | 1 |
| 20 | Beam buncher for heavy ions显示文摘 | LYNCH F J LEWIS I N BOLLINGER L M | 1979 | Nucl Instrum Methods1979，159，： | 1 |
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