|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Cryopreservation for delayed circulating tumor cell isolation is a valid strategy for prognostic association of circulating tumor cells in gastroesophageal cancer显示文摘AIM To demonstrate the feasibility of cryopreservation of peripheral blood mononuclear cells(PBMCs) for prognostic circulating tumor cell(CTC) detection in gastroesophageal cancer.METHODS Using 7.5 m L blood samples collected in EDTA tubes from patients with gastroesopheagal adenocarcinoma, CTCs were isolated by epithelial cell adhesion molecule based immunomagnetic capture using the Iso Flux platform. Paired specimens taken during the same blood draw(n = 15) were used to compare number of CTCs isolated from fresh and cryopreserved PBMCs. Blood samples were processed within 24 h to recover the PBMC fraction, with PBMCs used for fresh analysis immediately processed for CTC isolation. Cryopreservation of PBMCs lasted from 2 wk to 25.2 mo(median 14.6 mo). CTCs isolated from pre-treatment cryopreserved PBMCs(n = 43) were examined for associations with clinicopathological variables and survival outcomes.RESULTS While there was a significant trend to a decrease in CTC numbers associated with cryopreserved specimens(mean number of CTCs 34.4 vs 51.5, P = 0.04), this was predominately in samples with a total CTC count of > 50, with low CTC count samples less affected(P = 0.06). There was no significant association between the duration of cryopreservation and number of CTCs. In cryopreserved PBMCs from patient samples prior to treatment, a high CTC count(> 17) was associated with poorer overall survival(OS)(n = 43, HR = 4.4, 95%CI: 1.7-11.7, P = 0.0013). In multivariate analysis, after controlling for sex, age, stage, ECOG performance status, and primary tumor location, a high CTC count remained significantly associated with a poorer OS(HR = 3.7, 95%CI: 1.2-12.4, P = 0.03). CONCLUSION PBMC cryopreservation for delayed CTC isolation is a valid strategy to assist with sample collection, transporting and processing. | Daniel Brungs David Lynch Alison WS Luk Elahe Minaei Marie Ranson Morteza Aghmesheh Kara L Vine Martin Carolan Mouhannad Jaber Paul de Souza Therese M Becker | 2018 | World Journal of Gastroenterology2018，24，7： | 5 |
| 2 | Development of a regional climate model of the western Arctic 显示文摘 | Lynch A H Chapman W L Walsh J E | 1995 | Journal of Climate1995，8，： | 1 |
| 3 | Diagenesis of calcite cementation Frio Formation sandstones and its relationship to formation water chemistry 显示文摘 | Lynch F L Land L S | 1996 | Jour- nal of Sedimentary Research1996，66，3： | 1 |
| 4 | Recursive temporal-spatial information fusion with applications to target identification显示文摘 | Hong L Lynch A | 1993 | IEEE Transactions on Aerospace and Electronic Systems1993，29，： | 1 |
| 5 | 显示文摘 | JEONG S SESSLER J L LYNCH V | 2008 | J Am Chem Soc2008，130，： | 1 |
| 6 | Human capital investments and productivity显示文摘 | Black S Lynch L M | 1996 | American Economic Review1996，86，2： | 1 |
| 7 | Interactions between Fusarium culmorum and its potential biocontrol agent, Trichoderma harzianum, in a packed-bed, continuous-flow column reactor显示文摘 | Cheetham J L Bazin M J Lynch J M | 1997 | Enzyme and Microbial Technology1997，21，5： | 1 |
| 8 | Assessment of BLS skills, Optimizing use of instructor and manikin measures显示文摘 | LYNCH B EINSPRUCH E L NICHOL G | 2008 | Resuscitation2008，76，2： | 1 |
| 9 | Biotransformation of sevoflurane in dogs and rats 显示文摘 | Lynch S Napoli M D | 1981 | Anesth Analg1981，60，： | 1 |
| 10 | Social and Economic Exchange:Construct Development and Validation 显示文摘 | Shore L M Tetrick L E Lynch P Barksdale K | 2006 | Journal of Applied Social Psychology2006，36，4： | 1 |
| 11 | Organic matter,putative nannobacteria and theformation of ooids and hardgrounds显示文摘 | Folk R L Lynch F L | | Sedimentology0，48，21： | 1 |
| 12 | Simulation of Open Circuit Clinker Grinding显示文摘 | H Benzer L Ergun A Lynch | 2001 | Minerals Engineering2001，14，7： | 1 |
| 13 | In Search of Vocational and Technical Teacher Education显示文摘 | Lynch Richard L | 1996 | The Quality of Vocational Education1996，13，1： | 1 |
| 14 | Cetuximab and first- line taxane/carboplatin chemotherapy in advanced non- small- cell lung cancer: results of the randomized multicenter phase III trial BMS099显示文摘 | LYNCH TJ PATEL T DREISBACH L | 2010 | J Clin Oncol2010，28，6： | 1 |
| 15 | Pediatric obesity epidemiology 显示文摘 | Orsi CM Hale DE Lynch ]L | 2011 | Current Opinion in Endocrinology Diabetes &Obesity2011，18，： | 1 |
| 16 | CCR5 antagonists:bicyclic isoxazolidines as conformationally constrained N-(l)-substituted pyrolidines显示文摘 | LYNCH C L GENTRY A L HALE J J | | 0，，04： | 1 |
| 17 | Glucagon-like peptide 1 analogue therapy directly modulates innate immune-mediated inflammation in individuals with type 2 diabetes mellitus显示文摘 | Hogan AE Gaoatswe G Lynch L | 2014 | Dia- betologia2014，57，4： | 1 |
| 18 | Nursing Students'Experiences With and Strategic Approaches to Case-based Instruction:a Replication and Comparison Study Between Two Disciplines显示文摘 | Demarco R Hayward L Lynch M | 2002 | The Journal of Nursing Education2002，41，4： | 1 |
| 19 | Effectiveness of a 30-min CPR self-instruction program for lay responders: a controlled randomized study显示文摘 | LYNCH B EINSPRUCH E L NICHOL G | 2005 | Resuscitation2005，67，1： | 1 |
| 20 | Dry powder precursors of cubic liquid crystalline nanoparticles(cubosomes)显示文摘 | Spieer P T Small W B II Lynch M L Burns J L | 2002 | J Nanopart Res2002，4，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员