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| 1 | Discrimination of False Negative Results in RT-PCR Detection of SARS-CoV-2 RNAs in Clinical Specimens by Using an Internal Reference显示文摘Reverse transcription-polymerase chain reaction(RT-PCR)is an essential method for specific diagnosis of SARS-CoV-2 infection.Unfortunately,false negative test results are often reported.In this study,we attempted to determine the principal causes leading to false negative results of RT-PCR detection of SARS-CoV-2 RNAs in respiratory tract specimens.Multiple sputum and throat swab specimens from 161 confirmed COVID-19 patients were tested with a commercialfluorescent RT-PCR kit targeting the ORF1 ab and N regions of SARS-CoV-2 genome.The RNA level of a cellular housekeeping gene ribonuclease P/MRP subunit p30(RPP30)in these specimens was also assessed by RT-PCR.Data for a total of 1052 samples were retrospectively re-analyzed and a strong association between positive results in SARS-CoV-2 RNA tests and high level of RPP30 RNA in respiratory tract specimens was revealed.By using the ROC-AUC analysis,we identified Ct cutoff values for RPP30 RT-PCR which predicted false negative results for SARS-CoV-2 RT-PCR with high sensitivity(95.03%–95.26%)and specificity(83.72%–98.55%)for respective combination of specimen type and ampli-fication reaction.Using these Ct cutoff values,false negative results could be reliably identified.Therefore,the presence of cellular materials,likely infected host cells,are essential for correct SARS-CoV-2 RNA detection by RT-PCR in patient specimens.RPP30 could serve as an indicator for cellular content,or a surrogate indicator for specimen quality.In addition,our results demonstrated that false negativity accounted for a vast majority of contradicting results in SARS-CoV-2 RNA test by RT-PCR. | Yafei Zhang Changtai Wang Mingfeng Han Jun Ye Yong Gao Zhongping Liu Tengfei He Tuantuan Li Mengyuan Xu Luping Zhou Guizhou Zou Mengji Lu Zhenhua Zhang | 2020 | Virologica Sinica2020，35，6： | 5 |
| 2 | Neuroprotective effect of heat shock protein 70 Inhibition of Tau protein expression显示文摘BACKGROUND: Previous studies have shown that heat shock protein 70 (HSP70) hasneuroprotective effects by decreasing phosphorylation of Tau protein, thereby reducing theexpression of Tau protein and proper aggregation.OBJECTIVE: To observe and verify expressional changes of HSP70 and Tau in retinal ganglioncells following stretch injury to the right optic nerve in rats, and to determine the effect of heat stresspretreatment on HSP70 and Tau protein expressions.DESIGN, TIME AND SETTING: A randomized, controlled, animal experiment was performed at theLaboratory of Neurology Research Institute of the First Affiliated Hospital of Chongqing MedicalUniversity from March to June 2006.MATERIALS: Instant SABC immunohistochemistry kit, as well as mouse anti-HSP70 and rabbitanti-Tau polyclonal antibodies, were purchased from Wuhan Boster Bioengineering Limited, China.METHODS: A total of 57 male, Wistar rats were randomly assigned to 4 groups: control (n = 3);150-180 g stretch force was induced in the right optic nerves in stretch-only group (n = 18) toestablish optic nerve stretch injury model; heat stress was applied to 18 animals in heat-stresstreatment group; 18 rats in the heat-stress pretreatment plus stretch group were subjected toidentical stretch injury as stretch-only group after 24-hour heat-stress pretreatment. According tosacrifice time, the groups were assigned to 6 subgroups at different time points of 4, 8, and 16 hours,and 1,3, and 5 days, with 3 rats in each subgroup. No treatment was performed in the control groupexcept anesthesia.MAIN OUTCOME MEASURES: Morphological changes of optic nerves and retinal ganglion cellsfollowing stretch injury were observed by light microscopy following hematoxylin-eosin staining.HSP70 and Tau protein expression levels were observed in retinal ganglial cells from each groupusing im munohistochemistry.RESULTS: (1) Compared with the control group, morphological axonal and retinal ganglial cellchanges, as well as a decreased number of retinal ganglial cells, were identified in the stretch-onlygroup (P< 0.01). Pathological damage in optical nerve and retinal ganglial cells were not remarkablein the heat-stress pretreatment plus stretch group, with no statistical difference in the number ofretinal ganglial cells compared with the control group (P > 0.05). (2) Compared with the control groupsignificantly increased HSP70 expression in retinal ganglial cells occurred in the stretch-only,heat-stress treatment, and heat-stress pretreatment plus stretch groups (P < 0.05 or P < 0.01). Thepeak of HSP70 expression was earlier in the heat-stress pretreatment plus stretch group comparedwith the stretch-only and heat-stress treatment groups, and was expressed over a longer period oftime compared with the heat-stress treatment group. Compared with the control group, Tauexpression in the retinal ganglial cells rapidly increased 4-16 hours following stretch injury in thestretch-only group (P < 0.05 or P < 0.01), and obviously decreased in the heat-stress pretreatmentplus stretch group (P < 0.05 or P < 0.01).CONCLUSION: Tau expression increased following stretch injury, with an earlier expression peakthan HSP70, which indicated that stretch injury-induced HSP70 expression was not strong or quickenough to sufficiently protect the nerve. A much more enhanced HSP70 expression, with an earlierpeak and longer expression period, was observed in rats subjected to stretch injury following heatstress, which demonstrated that HSP70 exhibited neuroprotective functions by reducing abnormalaggregation of Tau. | Zhaohui He Xiaochuan Sun Feng Li Yong Jiang Haitao Wu Luping Zheng | 2009 | Neural Regeneration Research2009，4，12： | 1 |
| 3 | Optical topologicallattices of Bloch-type skyrmion and meron topologies显示文摘Optical skyrmions, quasiparticles that are characterized by the topologically nontrivial vectorial textures of optical parameters such as the electromagnetic field, Stokes parameters, and spin angular momentum, have aroused great attention recently. New dimensions for optical information processing, transfer, and storage have become possible, and developing multiple schemes for manipulating the topological states of skyrmions, thus, is urgent.Here we propose an approach toward achieving dynamic modulation of skyrmions via changing the field symmetry and adding chirality. We demonstrate that field symmetry governs the skyrmionic transformation between skyrmions and merons, whereas material chirality modulates the twist degree of fields and spins and takes control of the Néel-type–Bloch-type skyrmionic transition. Remarkably, the enantioselective twist of skyrmions and merons results from the longitudinal spin arising from the chirality-induced splitting of the hyperboloid in the momentum space. Our investigation, therefore, acts to enrich the portfolio of optical quasiparticles. The chiral route to topological state transitions will deepen our understanding of light–matter interaction and pave the way for chiral sensing, optical tweezers, and topological phase transitions in quantum matter. | Qiang Zhang Zhenwei Xie Peng Shi Hui Yang Hairong He Luping Du Xiaocong Yuan | 2022 | Photonics Research2022，10，4： | 1 |
| 4 | Erosion-Induced Inflammation on Coronary Plaque Stress/Strain and Flow Shear Stress Calculations Using OCT-Based FSI Computational Model显示文摘Plaque erosion,together with plaque rupture,is a common cause for acute coronary syndrome(ACS).Plaque erosion alone is responsible for about one third of the patients with ACS.Eroded plaque is defined as thrombosed,endothelium-absent and non-ruptured but often-inflamed plaques based on histological findings.Even though there is efficient imaging technologies to detect the eroded plaque in vivo and tailored treatment strategy has also been developed for ACScaused by erosion in clinics,the pathogenesis mechanisms that cause plaque erosion are not fully understood.It is widely postulated that thrombus formation and endothelial apoptosis(the precursors of plaque erosion)have closed association with biomechanical conditions in the coronary vessel.Revealing of the mechanical conditions in the eroded plaque could advance our knowledge in understanding the formation of plaque erosion.To this end,patient-specific OCT-based fluid-structure interaction(FSI)models were developed to investigate the plaque biomechanical conditions and investigate the impact of erosioninduced inflammation on biomechanical conditions.In vivo OCTand Biplane X-ray angiographic data of eroded coronary plaque were acquired from one male patient(age:64). OCT images were segmented manually with external elastic membrane contour and the trailing edge of the lipid-rich necrotic core(lipid)assumed to have positive remodeling ratio 1.1.Locations with luminal surface having direct contact with intraluminal thrombus on OCT images were identified erosion sites.Fusion of OCT and biplane X-ray angiographic data were performed to obtain the 3D coronary geometry.OCT-based FSI models with pre-shrink-stretch process and anisotropic material properties were constructed following previously established procedures.To reflect tissue weakening caused by erosion-induced inflammation,the material stiffness of plaque intima at the erosion site was adjust to one tenth of un-eroded fibrous plaque tissue.Three FSI models were constructed to investigate the impacts of inflammation and lipid component on plaque biomechanics:M1,without erosion(this means plaque intima at the erosion sites were not softened)and without inclusion of lipid component;M2,with erosion but no lipid;M3,with erosion and inclusion of lipid.FSI models were solved by ADINA to obtain the biomechanical conditions at peak blood pressure including plaque wall stress/strain(PWS/PWSn)and flow wall shear stress(WSS).The average values of three biomechanical conditions at the erosion sites and at the fibrous cap overlaying lipid component were calculated from three models for analysis.The results of M1 and M2 were compared to investigate the impact of erosion-induced inflammation on plaque biomechanics.Mean PWS value decreases from 49.98 kPa to 18.83 kPa(62.32%decrease)while Mean PWSn value increases from 0.123 1 to 0.138 4(12%increase)as the material stiffness becomes 10times soft.Comparing M2 and M3 at the cap sites,M3(with inclusion of lipid)will elevates mean PWS and PWSn values by48.59%and 16.09%,respectively.The impacts of erosion and lipid on flow shear stress were limited(<2%).To conclude,erosion-induced inflammation would lead to lower stress distribution but larger strain distribution,while lipid would elevate both stress and strain conditions.This shows the influence of erosion and lipid component has impacts on stress/strain cal-culations which are closely related to plaque assessment. | Liang Wang Luping He Rui Lv Xiaoya Guo Chun Yang Haibo Jia Dalin Tang | 2019 | 医用生物力学2019，34，A01： | 1 |
| 5 | MORNING GLOW OVER THE CITY显示文摘When Chinese read phrases such as guangmang wanzhang or jinxia wuyunlong,they may associate these descriptions with a beautiful morning glow and become intoxicated by the glorious scenes.In Chinese,countless characters and sentences can describe the natural beauty of the moment when the first glow of morning appears in the sky. | Wang Wei He Bingfu(Photos) Li Jidong(Photos) Wu Hui(Photos) Wu Luping(Photos) Zhang Yonghong(Photos) Zhang Ziliang(Photos) | 2021 | Beijing2021，，1： | 0 |
| 6 | Detector development at the Back-n white neutron source显示文摘Purpose Back-n is a white neutron beamline at China spallation neutron source,which was established in the year of 2018.It is a powerful facility for nuclear data measurement,neutron detector calibration,and radiation effect research.Method A series of detectors were built for different experiments,including beam monitoring,beam profile measurement,neutron induced secondaries(fission fragments,light charged particles and gamma)cross section measurement,and neutron resonance radiography,etc.A common digitization electronics and a cluster-based DAQ were developed for these detector systems.Most detectors have been employed at Back-n and serviced for experiments from the beginning of the beamline running.Results and conclusion As an overview of detectors of Back-n,the details of the detector design and the experiment performing are described in this paper.Some developing systems,e.g.,MTPC and B-MCP,are also included. | Fan Ruirui Li Qiang Bao Jie Li Yang Liu Rong Jiang Wei Ren Jie Zhang Qiwei Cao Ping Gu Minhao Ren Zhizhou Yi Han Tang Jingyu An Qi Bai Haofan Bai Jiangbo Chen Qiping Chen Yonghao Chen Zhen Cui Zengqi Fan Anchuan Feng Changqing Feng Fanzhen Gao Keqing Han Changcai Han Zijie He Guozhu He Yongcheng Hong Yang Hu Yiwei Huang Hanxiong Jia Weihua Jiang Haoyu Jiang Zhijie Jin Zhengyao Kang Ling Li Bo Li Chao Li Gong Li Jiawen Li Xiao Liu Jie Liu Shubin Luan Guangyuan Ning Changjun Qi Binbin Ruan Xichao Song Zhaohui Sun Kang Tan Zhixin Tang Shengda Wang Pengcheng Wang Zhaohui Wen Zhongwei Wu Xiaoguang Wu Xuan Xie Likun Yang Yiwei Yu Yongji Zhang Guohui Zhang Linhao Zhang Mohan Zhang Xianpeng Zhang Yuliang Zhang Yue Zhang Zhiyong Zhao Maoyuan Zhou Luping Zhou Zhihao Zhu Kejun The CSNS Back-n Collaboration | 2023 | Radiation Detection Technology and Methods2023，7，2： | 0 |
| 7 | Research on error accumulation control of three-dimensional adjustment with offset constraint显示文摘Background Currently,laser tracker is the primary instrument used to carry out three-dimensional position measurement in accelerator alignment.Theoretically,three-dimensional measuring data processed by three-dimensional adjustment are more rigorous,however,error accumulation is found in practice.Purpose In order to control error accumulation and further improve the measurement accuracy of accelerator alignment,this research introduces the laser alignment system into the activity of measurement and data processing.Methods A measurement scheme combining laser tracker and laser alignment system is proposed.To construct the constraint condition,the offset values from the measuring points to the laser straight-line datum were used.To carry out the three-dimensional adjustment with offset constraint,the laser tracker observations were used.Results A three-dimensional adjustment function model of laser tracker observations is given.The construction method of the constraint equation is researched,and the calculation formulas of the three-dimensional adjustment with offset constraint are derived.A 200 m linac tunnel control network is designed,using simulation measurement method,the measuring data of laser tracker and the offset values from the measuring points to the laser straight-line datum were generated.The simulated data are calculated by the method given in this paper and the result is analyzed.Conclusion Simulation result shows introducing the laser alignment system into laser tracker measurement and applying the three-dimensional adjustment with offset constraint can effectively suppress the error accumulation caused by long distance move station measurement. | Wang Xiaolong Kang Ling Dong Lan Li Bo Men Lingling Luo Tao Wang Tong Liang Jing He Zhenqiang Ke Zhiyong Ma Na Lu Shang Han Yuanying Yan Luping Zhang Luyan Liu Xiaoyang Yan Haoyue Li Chunhua Wu Lei | 2022 | Radiation Detection Technology and Methods2022，6，4： | 0 |
| 8 | CORRECTION:Correction to:Discrimination of False Negative Results in RT-PCR Detection of SARS-CoV-2 RNAs in Clinical Specimens by Using an Internal Reference显示文摘In the original version of Fig.41 and 4J,the cutoff lines were accidently shifted during figure layout.Figures 41 and 4J are re-drawn and provided below. | Yafei Zhang Changtai Wang Mingfeng Han Jun Ye Yong Gao Zhongping Liu Tengfei He Tuantuan Li Mengyuan Xu Luping Zhou Guizhou Zou Mengji Lu Zhenhua Zhang | 2020 | Virologica Sinica2020，35，6： | 0 |
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