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| 1 | Diversity characterization and association analysis of agronomic traits in a Chinese peanut (Arachis hypogaea L.) mini-core collection显示文摘Association mapping is a powerful approach for exploring the molecular basis of phenotypic variations in plants.A peanut(Arachis hypogaea L.)mini-core collection in China comprising 298 accessions was genotyped using 109 simple sequence repeat(SSR)markers,which identified 554 SSR alleles and phenotyped for 15 agronomic traits in three different environments,exhibiting abundant genetic and phenotypic diversity within the panel.A model-based structure analysis assigned all accessions to three groups.Most of the accessions had the relative kinship of less than 0.05,indicating that there were no or weak relationships between accessions of the mini-core collection.For 15 agronomic traits in the peanut panel,generally the Q t K model exhibited the best performance to eliminate the false associated positives compared to the Q model and the general linear model-simple model.In total,89SSR alleles were identified to be associated with 15 agronomic traits of three environments by the Q t K model-based association analysis.Of these,eight alleles were repeatedly detected in two or three environments,and 15 alleles were commonly detected to be associated with multiple agronomic traits.Simple sequence repeat allelic effects confirmed significant differences between different genotypes of these repeatedly detected markers.Our results demonstrate the great potential of integrating the association analysis and marker-assisted breeding by utilizing the peanut mini-core collection. | Huifang Jiang Li Huang Xiaoping Ren Yuning Chen Xiaojing Zhou Youlin Xia Jiaquan Huang Yong Lei Liying Yan Liyun Wan Boshou Liao | 2014 | Journal of Integrative Plant Biology2014，56，2： | 14 |
| 2 | Reduning injection for fever, rash, and ulcers in children with mild hand, foot, and mouth disease: a randomized controlled clinical study显示文摘OBJECTIVE: To assess the efficacy and safety of Reduning injection for fever, rash, and ulcers in children with mild hand, foot, and mouth disease(HFMD). METHODS: A stratified-block randomized, double-blind, parallel-controlled, and multicenter clinical trial was conducted with 360 patients in five hospitals across China: Quanzhou Children's Hospital, Shijiazhuang No. 5 Hospital, Shanghai Public Health Centre, Hunan Provincial Children's Hospital, and Kaifeng Children's Hospital. Patients were randomized into three groups with 120 in each. Group A was treated with Western Medicine, group B with Reduning injection, a Chinese herbal medicine, and group C with both Reduning injection and Western Medicine. Results were compared for treatment efficacy and safety on HFMD. The clinical outcomes were observed as follows: fever and onset time of antifebrile effect(time to bring the body temperature down ≥0.5℃ after medication); cumulative time for fever recovery(body temperature recovering to normal and lasting more than 24 h without medication); cumulative time for rash disappearance(without new rashes or ulcers appearing and the original ones fading away); and cumulative time for mouth ulcer disappearance. RESULTS: For the onset time of the antifebrile effect, there was no statistical difference between groups A and B(P>0.05) and groups B and C(P> 0.05). However, there was a statistical difference between groups A and C(P<0.05), and the effect in group C was the best. For the cumulative time for rash disappearance, there was no statistical difference between groups A and B(P>0.05). There were statistical differences between groups A and C, and groups B and C(P<0.05), and the effect in group C was the best. For the cumulative time for mouth ulcers disappearance, there were no statistical differences among the three groups(P>0.05). CONCLUSION: Reduning injection with Western Medicine for symptomatic treatment is most effective for mild HFMD. No adverse reactions were observed. | Guoliang Zhang Jie Zhao Liyun He Shiyan Yan Ziqiang Zhuo Haojie Zheng Yongping Mu Shuangjie Li Xi Zhang Jihan Huang Xiuhui Li Jianping Liu Hehe Wan Chaoyu Wei Wei Xiao | 2013 | Journal of Traditional Chinese Medicine2013，33，6： | 7 |
| 3 | Phenotypic identification of peanut germplasm for resistance to southern stem rot显示文摘Southern stem rot,caused by Sclerotium rolfsii Sacc.,is a destructive soil fungal disease of peanut in China and other countries.To evaluate resistant germplasm,a total of 256 peanut accessions were investigated on their resistance to southern stem rot in 3 environments by artificial inoculation.Variance analysis indicated that disease index was significantly influenced by environment,genotype and genotypeenvironment interactions.Peanut accessions of var.vulgaris type exhibited higher resistance to southern stem rot.Disease index was significantly negatively correlated with linoleic acid content,while positively correlated with oleic acid content.Six resistant accessions were identified,including Hua 28,Shandongzai,ICG 6326,Quanhua 7,Quanhua 9 and Guihua 836,with their disease indexes under 40 and mortality were less than 30%in the three environments.The identified resistant accessions showed the great potential to be applied in resistant peanut breeding,and would be good genetic resources for enhancing the resistance to southern stem rot. | Pengmin Fan Wanduo Song Yanping Kang Liyun Wan Yong Lei Dongxin Huai Yuning Chen Xin Wang Huifang Jiang Liying Yan Boshou Liao | 2020 | Oil Crop Science2020，5，4： | 3 |
| 4 | Genetic diversity of atoxigenic Aspergillus flavus isolates from peanut kernels in China显示文摘Aflatoxin contamination in peanuts is a major threat to public health, especially in tropical and subtropical regions of the world. Recently, the use of atoxigenic Aspergillus flavus strains as biological control agents prove to be effective in reducing aflatoxin contamination in crops. A total of 208 atoxigenic A. flavus isolates, collected from peanut kernels, were grouped into 7 deletion patterns by quadruplex PCR products of nor-1, ver-1, aflR and omtA genes. 49 SNPs, found in 1254 bp fragment of omtA gene, showed genetic variation of omtA among different A. flavus isolates. These isolates were assigned to either MAT1-1 type or MAT1-2 type with primers for each MAT locus. Thus, rich genetic diversity was found in the atoxigenic A. flavus isolates of peanut in China. The results indicated that quadruplex PCR would be an effective method for rapid screening of atoxigenic isolates with gene deletion in aflatoxin biosynthetic cluster. | Liying Yan Yanping Kang Yong Lei Liyun Wan Dongxin Huai Huifang Jiang Xiaoping Ren Boshou Liao | 2018 | Oil Crop Science2018，3，1： | 2 |
| 5 | Biocontrol potential of reducing aflatoxin contamination by an atoxigenic strain of Aspergillus flavus. isolated from peanut显示文摘 | Liying YAN Yanping KANG Yong LEI Liyun WAN Dongxin HUAI Huifang JIANG Xiaoping REN Boshou LIAO | 2016 | Oil Crop Science2016，1，1： | 2 |
| 6 | Direct detection of a single[4Fe-4S]cluster in a tungsten-containing enzyme:Electrochemical conversion of CO_(2) into formate by formate dehydrogenase显示文摘The conversion of CO_(2) into fuels and valuable chemicals is one of the central topics to combat climate change and meet the growing demand for renewable energy.Herein,we show that the formate dehydrogenase from Clostridium ljungdahlii(ClFDH)adsorbed on electrodes displays clear characteristic voltammetric signals that can be assigned to the reduction and oxidation potential of the[4Fe-4S]^(2+/+)cluster under nonturnover conditions.Upon adding substrates,the signals transform into a specific redox center that engages in catalytic electron transport.ClFDH catalyzes rapid and efficient reversible interconversion between CO_(2) and formate in the presence of substrates.The turnover frequency of electrochemical CO_(2) reduction is determined as 1210 s^(-1) at 25℃ and pH 7.0,which can be further enhanced up to 1786 s^(-1) at 50℃.The Faradaic efficiency at−0.6 V(vs.standard hydrogen electrode)is recorded as 99.3%in a 2-h reaction.Inhibition experiments and theoretical modeling disclose interesting pathways for CO_(2) entry,formate exit,and OCN−competition,suggesting an oxidation-state-dependent binding mechanism of catalysis.Our results provide a different perspective for understanding the catalytic mechanism of FDH and original insights into the design of synthetic catalysts. | Wenjin Li Yanxin Gao Xuan Sun Lei Wan Haishuo Ji Hang Luo Yao Tian Hao Song Geng Wu Liyun Zhang | 2023 | Carbon Energy2023，5，5： | 2 |
| 7 | Molecular cloning and characterization of a novel muscle adenylosuccinate synthetase, AdSSL1, from human bone marrow stromal cells显示文摘 | Hongying Sun Nan Li Xiaojian Wang Taoyong Chen Liyun Shi Lihuang Zhang Jianli Wang Tao Wan Xuetao Cao | 2005 | Molecular and Cellular Biochemistry2005，，1： | 1 |
| 8 | Three dimensionally braided carbon fabric- reinforced nylon composites prepared by in situ polymerization显示文摘 | Zheng Liyun Zhao Lixin Wan Yizao | 2005 | Carbon2005，43，： | 1 |
| 9 | Identification of major QTL for seed number per pod on chromosome A05 of tetraploid peanut(Arachis hypogaea L.)显示文摘The inheritance of pod-and seed-number traits(PSNT) in peanut(Arachis hypogaea L.) is poorly understood. In the present study, a recombinant inbred line(RIL) population of 188 lines was used to map quantitative trait loci(QTL) for number of seeds per pod(NSP),number of pods per plant(NPP), and numbers of one-, two-, and three-seeded pods per plant(N1 PP, N2 PP, and N3 PP) in four environments. A total of 28 consensus QTL and 14 single QTL were identified, including 11 major and stable QTL. Four major and stable QTL including qN3 PPA5.2, q N3 PPA5.4, qN3 PPA5.5, and qN3 PPA5.7 each explained 12.3%–33.0% of phenotype variation. By use of another integrated linkage map for the A5 group(hereafter referred to as INT A5 group), QTL for PSNT were located in seven intervals of 0.73–9.68 Mb in length on chromosome A05, and candidate genes underlying N3 PP were suggested. These findings shed light on the genetic basis of PSNT. Major QTL for N3 PP could be used as candidates for further positional cloning. | Yuning Chen Zhihui Wang Xiaoping Ren Li Huang Jianbin Guo Jiaojiao Zhao Xiaojing Zhou Liying Yan Huaiyong Luo Nian Liu Weigang Chen Liyun Wan Yong Lei Boshou Liao Dongxin Huai Huifang Jiang | 2019 | The Crop Journal2019，7，2： | 1 |
| 10 | Functional analyses of ethylene response factor JERF3 with the aim of improving tolerance to drought and osmotic stress in transgenic rice显示文摘 | Haiwen Zhang Wu Liu Liyun Wan Fang Li Liangying Dai Dingjun Li Zhijin Zhang Rongfeng Huang | 2010 | Transgenic Research2010，，5： | 1 |
| 11 | Progress on genetic and genomic research for enhanced oil content and quality in peanut显示文摘 | Boshou LIAO Yong LEI Li HUANG Silong CHEN Xiaoping REN Liying YAN Yurong LI Dongxin HUAI Xiaojing ZHOU Liyun WAN Yuning CHEN Wei HUA Huifang JIANG | 2016 | Oil Crop Science2016，1，1： | 1 |
| 12 | Functional analyses of ethylene response factor JERF3 with the aim of improving tolerance to drought and osmotic stress in transgenic rice显示文摘 | Haiwen Zhang Wu Liu Liyun Wan Fang Li Liangying Dai Dingjun Li Zhijin Zhang Rongfeng Huang | | 0，2010，： | 1 |
| 13 | siRNA screening of Rab GTPases reveal a role of Rab3b and Rab3c positive vesicles in cross presentation显示文摘Antigen cross-presentation in dendritic cells is a complex intracellular membrane transport process, but the underlying molecular mechanisms remain to be thoroughly investigated. | Liyun Zou, Jingran Zhou, Jinyu Zhang, Jingyi Li, Na Liu, Lingling Chai, Na Li, Ting Liu, Liqi Li, Zhunyi Xie, Hongli Liu, Ying Wan, Yuzhang Wu.Institute of Immunology,PLA, The Third Military Medical University, Chongqing 400038 | 2009 | 生物物理学报2009，25，S1： | 1 |
| 14 | Functional analyses of ethylene response factor JERF3 with the aim of improving tolerance to drought and osmotic stress in transgenic rice显示文摘 | Haiwen Zhang Wu Liu Liyun Wan Fang Li Liangying Dai Dingjun Li Zhijin Zhang Rongfeng Huang | 2010 | Transgenic Research2010，，5： | 1 |
| 15 | Functional analyses of ethylene response factor JERF3 with the aim of improving tolerance to drought and osmotic stress in transgenic rice显示文摘 | Haiwen Zhang Wu Liu Liyun Wan Fang Li Liangying Dai Dingjun Li Zhijin Zhang Rongfeng Huang | 2010 | Transgenic Research2010，，5： | 1 |
| 16 | Three dimensionally braided carbon fabric- reinforced nylon composites prepared by in situ polymerization显示文摘 | Zheng Liyun Zhao Lixin Wan Yizao | 2005 | Carbon2005，43，： | 1 |
| 17 | Clinical and antibody characteristics reveal diverse signatures of severe and non-severe SARS-CoV-2 patients显示文摘Background COVID-19 pandemic continues,clarifying signatures in clinical characters and antibody responses between severe and non-severe COVID-19 cases would benefit the prognosis and treatment.Methods In this study,119 serum samples from 37 severe or non-severe COVID-19 patients from the First People's Hospital of Yueyang were collected between January 25 and February 182020.The clinical features,antibody responses targeting SARS-CoV-2 spike protein(S)and its different domains,SARS-CoV-2-specific Ig isotypes,IgG subclasses,ACE2 competitive antibodies,binding titers with FcγIIa and FcγIIb receptors,and 14 cytokines were comprehensively investigated.The differences between severe and non-severe groups were analyzed using Mann–Whitney U test or Fisher’s exact test.Results Severe group including 9 patients represented lower lymphocyte count,higher neutrophil count,higher level of LDH,total bile acid(TBA)(P<1×10–4),r-glutaminase(P=0.011),adenosine deaminase(P<1×10–4),procalcitonin(P=0.004),C-reactive protein(P<1×10–4)and D-dimer(P=0.049)compared to non-severe group(28 patients).Significantly,higher-level Igs targeting S,different S domains(RBD,RBM,NTD,and CTD),FcγRIIa and FcγRIIb binding capability were observed in a severe group than that of a non-severe group,of which IgG1 and IgG3 were the main IgG subclasses.RBD-IgG were strongly correlated with S-IgG both in severe and non-severe group.Additionally,CTD-IgG was strongly correlated with S-IgG in a non-severe group.Positive RBD-ACE2 binding inhibition was strongly associated with high titers of antibody(S-IgG1,S-IgG3,NTD-IgG,RBD-IgA,NTD-IgA,and CTD-IgA)especially RBD-IgG and CTD-IgG in the severe group,while in the non-severe group,S-IgG3,RBD-IgG,NTD-IgG,and NTD-IgM were correlated with ACE2 blocking rate.S-IgG1,NTD-IgM and S-IgM were negatively associated with illness day in a severe group,while S-IgG3,RBD-IgA,CTD-IgA in the severe group(r=0.363,P=0.011)and S-IgG1,NTD-IgA,CTD-IgA in the non-severe group were positively associated with illness day.Moreover,GRO-α,IL-6,IL-8,IP-10,MCP-1,MCP-3,MIG,and BAFF were also significantly elevated in the severe group.Conclusion Antibody detection provides important clinical information in the COVID-19 process.The different signatures in Ig isotypes,IgG subclasses,antibody specificity between the COVID-19 severe and non-severe group will contribute to future therapeutic and preventive measures development. | Hongye Wang Dongshan Yan Ya Li Yanfei Gong Yulin Mai Bingxiang Li Xiaoyong Zhu Xinrui Wan Liyun Xie HuaKe Jiang Min Zhang Ming Sun Yufeng Yao Yongzhang Zhu | 2022 | Infectious Diseases of Poverty2022，11，1： | 0 |
| 18 | Recent advances in visible light-mediated chemical transformations of enaminones显示文摘Enaminones,which possesses both the nucleophilic enamine as well as electrophilic enone structures,are well known versatile building blocks in organic synthesis.Meanwhile,visible light-mediated reactions have emerged as useful synthetic strategy with enhanced sustainability.Around the last decade,various photochemical transformations of enaminones have been developed to construct cyclic or acyclic compounds.In this review,we describe the recent advances in visible light-mediated chemical transformations of enaminones.Detailed discussion on the reaction mechanism of the related reactions is given to provide guide to the reader.Finally,a summary on the existing challenges and the future outlook towards the development of practical photocatalytic reactions of enaminones is also presented. | Yu Han Liyun Zhou Chengyu Wang Shangti Feng Rong Ma Jie-Ping Wan | 2024 | Chinese Chemical Letters2024，35，2： | 0 |
| 19 | Development and validation of simple sequence repeat markers from Arachis hypogaea transcript sequences显示文摘Simple sequence repeats(SSRs) are important molecular markers for assessing genetic diversity in Arachis hypogaea L. and many other crops and constructing genetic linkage maps for important agricultural traits. In this study, 29,357 potential SSRs were identified in 22,806 unigenes assembled from A. hypogaea transcript sequences. Of these unigenes, 1883 and 4103 were annotated and assigned in Kyoto Encyclopedia of Genes and Genomes Orthology and Eukaryotic Orthologous Groups databases, respectively. Among the SSR motifs, mono-(19,065; 64.94%) and trinucleotide(5033; 17.14%) repeats were the most common, and the three most dominant motifs were A/T(18,358; 62.54%), AG/CT(2804;9.55%), and AAG/CTT(1396; 4.76%). Polymerase chain reaction(PCR) primer pairs were designed for 4340 novel SSR markers and 210 new SSRs were validated using 24 A. hypogaea varieties. Of the 210, 191(91%) yielded PCR products, with 37(18%) identifying polymorphisms. The 37 polymorphic SSR markers detected 146 alleles(2–10 alleles per locus), and the average polymorphic information content was 0.403(with a range of 0.077 to0.819). The new SSRs enrich the current marker resources for A. hypogaea and may also be useful for genetic diversity analysis, functional genomics research, and molecular breeding. | Houmiao Wang Yong Lei Liying Yan Liyun Wan Yan Cai Zefeng Yang Jianwei Lv Xiaojie Zhang Chenwu Xu Boshou Liao | 2018 | The Crop Journal2018，6，2： | 0 |
| 20 | Hydrogenase as the basis for green hydrogen production and utilization显示文摘Hydrogenase is a paradigm of highly efficient biocatalyst for H_(2) production and utilization evolved in nature. A dilemma is that despite the high activity and efficiency expected for hydrogenases as promising catalysts for the hydrogen economy, the poor oxygen tolerance and low yield of hydrogenases largely hinder their practical application. In these years, the enigmas surrounding hydrogenases regarding their structures, oxygen tolerance, mechanisms for catalysis, redox intermediates, and proton-coupled electron transfer schemes have been gradually elucidated;the schemes, which can well couple hydrogenases with other highly efficient(in)organic and biological catalysts to build novel reactors and drive valuable reactions, make it possible for hydrogenases to find their niches. To see how scientists put efforts to tackle this issue and design novel reactors in the fields where hydrogenases play crucial roles, in this review,recent advances were summarized, including different strategies for protecting enzyme molecules from oxygen, enzyme-based assembling systems for H_(2) evolution in the photoelectronic catalysis, enzymatic biofuel cells for H_(2) utilization and storage and the efficient electricity-hydrogen-carbohydrate cycle for high-purity hydrogen and biofuel automobiles. Limitations and future perspectives of hydrogenasebased applications in H_(2) production and utilization with great impact are discussed. In addition, this review also provides a new perspective on the use of biohydrogen in healthcare beyond energy. | Haishuo Ji Lei Wan Yanxin Gao Ping Du Wenjin Li Hang Luo Jiarui Ning Yingying Zhao Huangwei Wang Lixin Zhang Liyun Zhang | 2023 | Journal of Energy Chemistry2023，，10： | 0 |
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