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| 1 | Actin cytoskeleton-dependent pathways for ADMA-induced NF-κB activation and TGF-β high expression in human renal glomerular endothelial cells显示文摘不对称的 dimethylarginine (ADMA ) ,一个内长的氮的氧化物 synthase 禁止者,被认为是在长期的肾疾病(CKD ) 的前进的一个独立风险因素。它能由增加转变生长因素(TGF )-1 表示导致肾纤维变性,但是它的分子的机制是不清楚的。现在的学习的目的是在人的肾的 glomerular endothelial 房间(HRGEC ) 在导致 ADMA 的 TGF-1 高表示调查肌动朊细胞骨架的角色。压力纤维的结构被 immunofluorescence 设想,原子 factor-B (NF-B )DNA 有约束力的活动被 electrophoretic 活动性移动试金估计, TGF-1 表示被西方的污点分析估计。结果证明 ADMA 导致了压力纤维, NF-B 的 DNA 绑定,和 TGF-1 的增加的表示的汇编。当肌动朊细胞骨架的动力学被使解聚肌动朊的代理人 cytochalasin 使不安时, D 和稳定肌动朊的代理人 jasplakinolide,或压力纤维的脱离由 nicotineamide 腺嘌 dinucleotide 磷酸盐 oxidase 禁止者 apocynin 和 p38 绑激活 mitogen 的蛋白质 kinase 禁止者 SB203580, NF-B 的导致 ADMA 的 DNA 绑定和 TGF-1 表示被禁止。这些结果在 HRGEC 在导致 ADMA 的 NF-B 激活和 TGF-1 高表示揭示了肌动朊细胞骨架依赖者机制。肌动朊细胞骨架的特定的指向可以是有用策略在 CKD 阻止激活 ADMA 的肾纤维变性。 | Liyan Wang Dongliang Zhang Junfang Zheng Yiduo Feng Yu Zhang Wenhu Liu | 2012 | Acta Biochimica et Biophysica Sinica2012，44，11： | 9 |
| 2 | Analysis of genetic architecture and favorable allele usage of agronomic traits in a large collection of Chinese rice accessions显示文摘Genotyping and phenotyping large natural populations provide opportunities for population genomic analysis and genome-wide association studies(GWAS). Several rice populations have been re-sequenced in the past decade;however, many major Chinese rice cultivars were not included in these studies. Here, we report large-scale genomic and phenotypic datasets for a collection mainly comprised of 1,275 rice accessions of widely planted cultivars and parental hybrid rice lines from China. The population was divided into three indica/Xian and three japonica/Geng phylogenetic subgroups that correlate strongly with their geographic or breeding origins. We acquired a total of 146 phenotypic datasets for 29 agronomic traits under multi-environments for different subpopulations. With GWAS, we identified a total of 143 significant association loci, including three newly identified candidate genes or alleles that control heading date or amylose content. Our genotypic analysis of agronomically important genes in the population revealed that many favorable alleles are underused in elite accessions, suggesting they may be used to provide improvements in future breeding efforts. Our study provides useful resources for rice genetics research and breeding. | Xiuxiu Li Zhuo Chen Guomin Zhang Hongwei Lu Peng Qin Ming Qi Ying Yu Bingke Jiao Xianfeng Zhao Qiang Gao Hao Wang Yunyu Wu Juntao Ma Liyan Zhang Yongli Wang Lingwei Deng Shanguo Yao Zhukuang Cheng Diqiu Yu Lihuang Zhu Yongbiao Xue Chengcai Chu Aihong Li Shigui Li Chengzhi Liang | 2020 | Science China(Life Sciences)2020，63，11： | 8 |
| 3 | Performance measures for management of chronic heart failure patients with acute coronary syndrome in China: results from the Bridging the Gap on Coronary Heart Disease Secondary Prevention in China (BRIG) Project显示文摘 | WANG Na ZHAO Dong LIU Jing LIU Jun Cheuk-Man Yu WANG Wei SUN Jia-yi LIYan CHEN Bu-xing | 2013 | Chinese Medical Journal2013，，14： | 7 |
| 4 | Development of an Inactivated Iridovirus Vaccine Against Turbot Viral Reddish Body Syndrome显示文摘Turbot(Scophthalmus maximus L.) reddish body iridovirus(TRBIV) was propagated in turbot fin cells(TF cells) and inactivated as the TRBIV vaccine with its protection efficiency evaluated in this study.TF cells were cultured in 10% bovine calf serum(BCS)-containing MEM medium(pH7.0) at 22℃,in which TRBIV propagated to a titer as high as 105.6 TCID50 mL-1.The TRBIV was inactivated with 0.1% formalin and formulated with 0.5% aluminum hydroxide.The inactivated vaccine caused neither cytopathogenic effect(CPE) on TF cells nor pathogenic effect on turbots.After being administered with the vaccine twice via muscle injection,the turbot developed high-tittered TRBIV neutralizing antibodies in a dose-dependent manner.The vaccine protected the turbot from dying with an immunoprotection rate of 83.3% as was determined via subcutaneous vaccination in the laboratory and 90.5% via bath vaccination in turbot farms,respectively.The inactivated vaccine was very immunogenic,efficiently preventing tur-bot from death.It holds the potential of being applied in aquaculture. | FAN Tingjun HU Xiuzhong WANG Liyan GENG Xiaofen JIANG Guojian YANG Xiuxia YU Miaomiao | 2012 | Journal of Ocean University of China2012，11，1： | 5 |
| 5 | Hybrid Phase-controlled Circuit Breaker with Switch System Used in the Railway Auto-passing Neutral Section with an Electric Load显示文摘Constrained by the characteristics of neutral sec- tions (NS) and traditional vacuum circuit breakers, previous phase-controlled strategies have a long power supply dead time, it is difficult to realize a continuous power supply to the auxiliary power system. The dead time can be reduced by using the ground automatic convert method, hybrid phase-controlled technologies can in theory completely eliminate inrush currents. In this paper, a new system based on hybrid phase-controlled switches is described and termed ground-switching passing neutral section system (GPNSS). The principle for restraining inrush currents is analyzed and strategies are carried out with the dead time limited to 5 ms. The characteristics of the vacuum circuit breaker are illustrated and the closing time window of the transformer is quantified. Through the use of mechanical switches and power electronics, the auxiliary power system may be continuously pow- ered. The prototype system is implemented and experimentally tested in the laboratory. | Shuo Wang Liyan Zhang Guanghui Yu Enyuan Dong Jiyan Zou Yu Cong Yu Tian | 2019 | CSEE Journal of Power and Energy Systems2019，5，4： | 4 |
| 6 | EFFICIENT BOX-CONSTRAINED TV-TYPE-l^1 ALGORITHMS FOR RESTORING IMAGES WITH IMPULSE NOISE显示文摘 | Liyan Ma Michael K. Ng Jian Yu Tieyong Zeng | 2013 | Journal of Computational Mathematics2013，31，3： | 4 |
| 7 | Characterization of a Novel Anti-DR5 Monoclonal Antibody WD1 with the Potential to Induce Tumor Cell Apoptosis显示文摘TNF-related apoptosis-inducing ligand (TRAIL) is a TNF family member capable of inducing apoptosis. Death receptor 5 (DR 5) is a key receptor of TRAIL and plays an important role in TRAIL-induced apoptosis. To prepare monoclonal antibodies (mAbs) against DR5,cDNA encoding soluble DR5 (sDR5) was firstly amplified by reverse transcriptase-polymerase chain reaction (RT-PCR) with specific primers,and then inserted into a prokaryotic expression vector pET-30a. The recombinant plasmid was expressed in Escherichia coli strain BL21 (DE3),and sDR5 was purified by nickel affinity chromatography. As an antigen,sDR5 was used to immunize mice. Hybridomas secreting antibodies against sDR5 were identified. One positive clone was selected to produce antibody,WD1. ELISA and immunofluorescence demonstrated that WD1 could bind recombinant sDR5 and membrane-bound DR5 (mDR5) on Jurkat and Molt-4 cells. ATPLite assays showed that Jurkat and Molt-4 cells were sensitive to the antibody in a dose dependent manner. The Annexin V/PI assays and Giemsa's staining both showed that WD1 could induce Jurkat cell apoptosis efficiently. Transient transfection of 293T cells and indirect immunofluorescence assay demonstrated that mAb (WD1) couldn't cross-react with DR4. Our findings indicated that the novel antibody,WD1 could act as a direct agonist,bind DR5 characteristically,and initiate efficient apoptotic signaling and tumor regression. Thus,WD1 would be a leading candidate for potential cancer therapeutics. | Jing Wang Zhou Lin Chunxia Qiao Ming Lv Ming Yu He Xiao Qingyang Wang Liyan Wang Jiannan Feng Beifen Shen Yuanfang Ma Yan Li | 2008 | Cellular & Molecular Immunology2008，5，1： | 4 |
| 8 | Synthesis and characterization of phosphotungstic acid/ activated carbon as a novel ultrasound oxidative desulfurization catalyst显示文摘有各种各样的交流尺寸或 PTA 内容的 Phosphotungstic acid/activated 碳(PTA/AC ) 催化剂被 N2 physisorption 综合了并且描绘, X 光检查衍射, Fourier 变换规划的红外线的光谱学和温度氨的解吸附作用。这些催化剂然后被 ultrasoundassisted 以 dibenzothiophene (DBT ) 的移动评估氧化 desulfurization 过程。结果证明显然与交流的减少增加的 DBT 变换支持尺寸和 PTA 内容的增加。在在交流上支持 PTA 以后, DBT 变换能被 38.9% 为 10 min 在超声照耀以后改进。另外, PTA/AC 的稳定性测试证明 PTA/AC 的催化氧化活动将近为 20 min 在超声照耀以后被使经常,它成为在帮助超声的氧化 desulfurization 过程使用是有希望的催化剂。 | Liyan LIU Yu ZHANG Wei TAN | 2013 | Frontiers of Chemical Science and Engineering2013，7，4： | 3 |
| 9 | Preparation and luminescence properties of orange-red Ba3Y4O9:Sm3+phosphors显示文摘A novel orange-red emitting Ba_3 Y_4 O_9:Sm^(3+) phosphors were prepared by a high temperature solidstate reaction in air. X-ray diffraction(XRD), photoluminescence spectra, fluorescence decay and temperature-dependent emission spectra were utilized to characterize the structure and luminescence properties. The results show that the excitation spectrum includes a series of linear peaks at350, 367, 382, 410, 424, 445, 470 and 495 nm, respectively. Under 410 nm excitation, the emission peaks were located at 574 nm(~4 G_(5/2)-~6 H_(5/2)), 608 nm(~4 G_(5/2)-~6 H_(7/2)),659 nm(~4 G_(5/2)-~6 H_(9/2)) and722 nm(~4 G_(5/2)-~6 H_(11/2)), respectively. The concentration quenching occurs when x equals 0.08 for Ba_3 Y_(4-x)O_9:xSm^(3+) phosphor and its mechanism is ascribed to the dipole-dipole interaction. The chromaticity coordinates of Ba_3 Y_(3.92)O_9:0.08 Sm^(3+) phosphor are in the orange-red region. The temperature-dependent study shows that this phosphor has excellent luminescence thermal-stability.And the luminescence intensity of Ba_3 Y_(3.92)O_9:0.08 Sm^(3+) phosphor at 473 K only declines by about25.75% of its initial intensity. The experimental data indicate that Ba_3 Y_4 O_9:Sm^(3+) phosphor may be promising as an orange-red emitting phosphor for white light emitting diodes. | Jiayu Li Ran Pang Zhan Yu Liyan Liu Haiyan Wu Huimin Li Lihong Jiang Su Zhang Jing Feng Chengyu Li | 2018 | Journal of Rare Earths2018，36，7： | 3 |
| 10 | Nano-and microphytoplankton community characteristics in brown tide bloom-prone waters of the Qinhuangdao coast,Bohai Sea, China显示文摘Since 2009, the newly recorded species Aureococcus anophagefferens has successively induced brown tides in the Qinhuangdao coastal waters of the Bohai Sea. Here, we focus on the phytoplankton community structure in this region and try to point out the biological background characteristics of the outbreaks of successive brown tides. We analyzed species composition,cell abundance, diversity indices and size-fractionated chlorophyll a(Chl-a) concentration monthly from March 2013 to January2014. The phytoplankton community, as observed by microscopy, underwent succession from nano-celled chained diatoms to dinoflagellates in the spring and summer, and then to micro-celled diatoms and nano-celled chained diatoms as the most prominent groups in the subsequent autumn and winter. Canonical correspondence analysis revealed that changes in the abundance of diatoms were related to nutrient availability, especially of nitrogen, while water temperature was the crucial factor influencing the abundance of dinoflagellates and A. anophagefferens. Considering the contribution of nano-celled chained species to the micro-sized Chl-a,small phytoplankton(<20 μm) composed the majority of total Chl-a and were generally abundant during our study. Abundant and diverse small phytoplankton seemed to serve as a background or seedbank for the formation of blooms of A. anophagefferens and other small-celled algae. | XU Xin YU ZhiMing HE LiYan CHENG FangJin CAO XiHua SONG XiuXian | 2017 | Science China Earth Sciences2017，60，6： | 3 |
| 11 | White blood cell count and the incidence of hyperuricemia:insights from a community-based study显示文摘Hyperuricemia(HUA)is a risk factor for chronic kidney disease(CKD).The relationship between HUA and white blood cell(WBC)count remains unknown.A sampling survey for CKD was conducted in Sanlin community in 2012 and 2014.CKD was defined as proteinuria in at least the microalbuminuric stage or an estimated GFR of 60 mL/(min·1.73 m2).HUA was defined as serum uric acid>420µmol/L in men and>360µmol/L in women.This study included 1024 participants.The prevalence of HUA was 17.77%.Patients with HUA were more likely to have higher levels of WBC count,which was positively associated with HUA prevalence.This association was also observed in participants without CKD,diabetes mellitus,hyperlipidemia,or obesity.Multivariate logistic regression analysis showed that WBC count was independently associated with the risk for HUA in male and female participants.Compared with participants without HUA,inflammatory factors such as high-sensitivity C-reactive protein,tumor necrosis factor-α,and interleukin 6 increased in participants with HUA.Hence,WBC count is positively associated with HUA,and this association is independent of conventional risk factors for CKD. | Jian Liu Pingyan Shen Xiaobo Ma Xialian Yu Liyan Ni Xu Hao Weiming Wang Nan Chen | 2019 | Frontiers of Medicine2019，13，6： | 3 |
| 12 | Structural analyses of Legionella LepB reveal a new GAP fold that catalytically mimics eukaryotic RasGAP显示文摘Rab GTPases 是多样的细菌的病原体的新兴的目标。这里,我们执行 LepB 的生物化学、结构的分析,从 Legionella pneumophila 的一个 Rab 激活 GTPase 蛋白质(差距) 受动器。我们印射 LepB 差距领域到 GAP 领域比正规真核细胞的 TBC 差距和细菌的 RabGAP 受动器的最新识别的 VirA/EspG 家庭高是与一项催化活动特定的 Rab1 的残余 313-618 和表演。彻底的变化分析作为精氨酸手指,而是没有催化地必要的夫酸安残余识别 Arg444。独自一个的 LepB 313-618 和在有 Rab1-GDP-AlF 3 的建筑群的 Legionella drancourtii LepB 的 GAP 领域的水晶结构支持 Arg444 的催化角色,并且进一步也揭示 3D 体系结构和从所有已知的差距不同的一个 GTPase 有约束力的模式。Glu449,在结构上等价物到在 apo-LepB 的 TBC RabGAP 夫酸安手指,在 Rab1 绑定之上经历一个激烈的运动,它导致通过一个强壮的离子的相互作用向 GDP-AlF 3 扭动的 Rab1 Gln70 方面链。这 conformationally 重排的 Gln70 充当催化 cis 夫酸安,因此揭开为 LepB 的意外象 RasGAP 一样催化机制。我们的研究加亮 RabGAPs 的非凡的结构、催化的差异,特别地那些从细菌的病原体。 | Qin Yu Liyan Hu Qing Yao Yongqun Zhu Na Dong Da-Cheng Wang Feng Shao | 2013 | Cell Research2013，23，6： | 3 |
| 13 | Biosynthesis of antibiotic chuangxinmycin from Actinoplanes tsinanensis显示文摘Chuangxinmycin is an antibiotic isolated from Actinoplanes tsinanensis CPCC 200056 in the1970 s with a novel indole-dihydrothiopyran heterocyclic skeleton. Chuangxinmycin showed in vitro antibacterial activity and in vivo efficacy in mouse infection models as well as preliminary clinical trials.But the biosynthetic pathway of chuangxinmycin has been obscure since its discovery. Herein, we report the identification of a stretch of DNA from the genome of A. tsinanensis CPCC 200056 that encodes genes for biosynthesis of chuangxinmycin by bioinformatics analysis. The designated cxn cluster was then confirmed to be responsible for chuangxinmycin biosynthesis by direct cloning and heterologous expressing in Streptomyces coelicolor M1146. The cytochrome P450 CxnD was verified to be involved in the dihydrothiopyran ring closure reaction by the identification of seco-chuangxinmycin in S. coelicolor M1146 harboring the cxn gene cluster with an inactivated cxn D. Based on these results, a plausible biosynthetic pathway for chuangxinmycin biosynthesis was proposed, by hijacking the primary sulfur transfer system for sulfur incorporation. The identification of the biosynthetic gene cluster of chuangxinmycin paves the way for elucidating the detail biochemical machinery for chuangxinmycin biosynthesis, and provides the basis for the generation of novel chuangxinmycin derivatives by means of combinatorial biosynthesis and synthetic biology. | Yuanyuan Shi Zhibo Jiang Xingxing Li Lijie Zuo Xuan Lei Liyan Yu Linzhuan Wu Jiandong Jiang Bin Hong | 2018 | Acta Pharmaceutica Sinica B2018，8，2： | 2 |
| 14 | An Improvement on Particle Swarm Optimization显示文摘 | QIAO Liyan PENG Xiyuan PENG Yu | 2006 | Chinese Journal of Electronics2006，15，2： | 2 |
| 15 | Structural characterization and antioxidant activity of a polysaccharide from the fruiting bodies of cultured Cordyceps militaris显示文摘 | Rongmin Yu Wei Yang Liyan Song Chunyan Yan Zhang Zhang Yu Zhao | 2007 | Carbohydrate Polymers2007，，4： | 2 |
| 16 | N,P-codoped porous carbon derived from chitosan with hierarchical N-enriched structure and ultra-high specific surface Area toward high-performance supercapacitors显示文摘In this work,a facile'carbonization-activation'strategy is developed to synthesize N,P-codoped hierarchical porous carbon.Phosphoric acid is innovatively introduced during the hydrothermal process to achieve in-situ P doping as well as create abundant pores,and the employment of sodamide is of vital importance to simultaneously serve as activating agent and N-source to succeed a high-level N doping.Thus,the obtained samples exhibit a unique three-dimensional hierarchical structure with an ultra-high specific surface area(3646 m^(2)g^(-1))and ultra-high N-doping level(9.81 at.%).Computational analyses confirm that N,P co-doping and higher N content can enhance active sites and widen potential differences of carbon materials to improve their capacitance.The as-prepared carbon materials demonstrate superior electrochemical performances,such as an ultra-high capacitance of 586 Fg^(-1)at 1 Ag^(-1),a superior rate capability of 409 Fg^(-1)at 20 Ag^(-1),and excellent long-term stability of 97%capacitance retention after10,000 cycles in 6 M KOH.Moreover,an assembled symmetric supercapacitor delivers a high energy density of 28.1 Wh kg^(-1)at the power density of 450 W kg^(-1)in 1 M Na_(2)SO_(4),demonstrating a great potential for applications in supercapacitors. | Xipeng Xin Na Song Ruiming Jia Bingnan Wang Hongzhou Dong Shuai Ma Lina Sui Yingjie Chen Qian Zhang Lifeng Dong Liyan Yu | 2021 | Journal of Materials Science & Technology2021，，29： | 2 |
| 17 | A Convex Approach for Local Statistics Based Region Segmentation显示文摘 | MA Liyan YU Jian | 2012 | Chinese Journal of Electronics2012，21，4： | 2 |
| 18 | Validated LC–MS/MS method for determination of YH-8, a novel PKnB inhibitor, in rat plasma and its application to pharmacokinetic study显示文摘(E)-Methyl-4-aryl-4-oxabut-2-enoate(YH-8) is a novel PKn B protein kinase inhibitor with good anti-tuberculosis activity. To evaluate its pharmacokinetics in rats, a sensitive and selective high performance liquid chromatography–tandem mass spectrometric(LC–MS/MS) method has been developed and validated for the quantification of YH-8 in rat plasma for the first time. Samples were pre-treated using a liquid–liquid extraction with ethyl acetate and the chromatographic separation was performed on a C18 column by gradient elution with methanol–water as the mobile phase. YH-8 was detected using a tandem mass spectrometer in positive selected reaction monitoring(SRM) mode. Method validation revealed good linearity over the range of1–500 ng/m L for YH-8 with a lower limit of quantification(LLOQ) of 1 ng/m L. Intra- and inter-day precision of YH-8 assay in rat plasma samples were 2.0%–6.8%, with accuracy of the method being 100.69%–106.18%.Stability test showed that when spiked into rat plasma, YH-8 was stable for 12 h at room temperature, for up to15 days at -70℃, and after three freeze-thaw cycles. Extracted samples were found to be stable over 12 h in an auto-sampler. The method was successfully applied to the pharmacokinetic study of YH-8 in rats after oral administration at 100 mg/kg and 200 mg/kg. | Qianqian Zhai Jing Pang Guoqing Li Congran Li Xinyi Yang Liyan Yu Yucheng Wang Jian Li Xuefu You | 2015 | Acta Pharmaceutica Sinica B2015，5，5： | 2 |
| 19 | Classification of duck hepatitis virus into three genotypes based on molecular evolutionary analysis显示文摘 | Liyan Wang Meng Pan Yu Fu Dabing Zhang | 2008 | Virus Genes2008，，1： | 1 |
| 20 | A Simple Method to Synthesize Multi-branched Carbon Fibers Using Cupric Chloride Aqueous Solution as Catalyst Precursor显示文摘Carbon fibers with multi-branched structures were synthesized by chemical vapor deposition method using cupric chloride as catalyst precursor and acetylene as carbon source at different reaction temperatures.Effects of water vapor and reaction temperature on the growth mode of carbon fibers were investigated.Experimental results demonstrate that initial reaction conditions and temperature are key factors for the formation of different carbon materials.Carbon fibers with typical multi-branched structures can be obtained at 450 C when cupric chloride solution was used as catalyst precursor.X-ray diffraction,field emission scanning electron microscopy,and transmission electron microscopy are used to characterize carbon materials,and the growth mechanisms of multi-branched carbon fibers were discussed. | Qiuxiang Wang Qian Zhang Liping Chen Liyan Yu Lifeng Dong | 2014 | Journal of Materials Science & Technology2014，30，9： | 1 |
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