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| 1 | Human amnion epithelial cells can be induced to differentiate into functional insulin-producing cells显示文摘胰腺的小岛移植证明了长期的胰岛素独立可以在受不了糖尿病 mellitus 类型 1 的病人被完成。然而,小岛织物的有限可获得性意味着对葡萄糖应答的生产胰岛素的房间的新来源被要求。这里,我们显示出那人的羊膜上皮的房间(HAEC ) 能被导致在 vitro 区分进功能的生产胰岛素的房间。在区别的正式就职以后, HAEC 表示了多重胰腺的 β-cell 基因,包括胰岛素,胰十二指肠 homeobox-1,配对的盒子基因 6, NK2 抄写因素相关的地点 2,小岛 1, glucokinase,和葡萄糖 transporter-2,和以响应另外的细胞外的刺激的一种调整葡萄糖的方式的释放 C 肽。进导致 streptozotocin 的糖尿病的 C57 老鼠的导致的 HAEC 的移植颠倒了多糖症,恢复了身体重量,并且维持了为 30 d 的 euglycemia。这些调查结果显示 HAEC 可以是为在类型 1 糖尿病的房间代替治疗的新来源。 | Yanan Hou | 2008 | Acta Biochimica et Biophysica Sinica2008，40，9： | 25 |
| 2 | Cancer stem-like cells can be isolated with drug selection in human ovarian cancer cell line SKOV3显示文摘为药抵抗的肿瘤的发展的一个新兴的模型利用作为癌症干细胞(CSC ) 或开始癌症的房间(用户信息控制系统) 知道的自我更新的恶意的祖先的一个水池。这研究的目的是从卵巢的癌症房间线 SKOV3 宣传如此的用户信息控制系统。SKOV3 范围房间在与表皮的生长因素补充的没有浆液的文化系统用 40.0 mol/l cisplatin 和 10.0 mol/l paclitaxel 被选择,基本成纤维细胞生长因素,白血病禁止的因素或标准包含浆液的系统。这些房间在药选择(cisplatin 和 paclitaxel ) 和没有浆液的文化系统下面形成了非支持者范围。选择范围房间对 cisplatin, paclitaxel, adriamycin,和 methotrexate 更抵抗。重要地,范围房间有自强的性质,与干细胞基因 Nanog, Oct4, sox2, nestin, ABCG2, CD133,和干细胞因素受体的高表示 CD117 (c 工具包) 。一致地,流动 cytometric 分析表明范围房间比区分的房间(33%) 有 CD133+/CD117+-positive 房间(71%) 的一个高得多的百分比。而且, SKOV3 范围房间是更多的 tumorigenic。而且, cDNA microarray 和随后的本体论的分析表明分类基因的一个大比例与 angiogenesis,细胞外的矩阵,调停 integrin 的发信号小径,房间粘附,和房间增长有关。从在这个文化系统下面的 SKOV3 房间线的 subpopulation 隔离出现一在为以他们的幸存,自强,和 chemoresistance 学习卵巢的 CSC,并且为开发明确地防碍卵巢的 CSC 的治疗学的药的 vitro 模型合适。 | Li Ma Dongmei Lai Te Liu Weiwei Cheng Lihe Guo | 2010 | Acta Biochimica et Biophysica Sinica2010，42，9： | 18 |
| 3 | Transformation of human amniotic epithelial cells into neuron-like cells in the microenvironment of traumatic brain injury in vivo and in vitro显示文摘Survival and differentiation of transplanted cells is closely related to the local microenvironment.The present study cultured human amniotic epithelial cells(HAECs) in a simulated microenvironment in vitro comprising RPMI 1640 culture medium and the solution extracted from injured brain tissues.Some HAECs were round,triangular in form or irregularly shaped,with extended neuron-like processes;some of the processes were interconnected,representing neuron-like morphology and some HAECs were microtubule-associated protein 2-positive.HAECs survived for at least 4 weeks following transplantation into the center and edges of the trauma focus with traumatic brain injury,and were microtubule-associated protein 2-positive.Moreover,the motor function of rat hind limbs was significantly improved. | Yi Lu Guozhen Hui Zhiyuan Wu Tianjin Liu Xunhe Ji Lihe Guo | 2011 | Neural Regeneration Research2011，6，10： | 10 |
| 4 | Monoamine alterations and rotational asymmetry in a rat model of Parkinson's disease following lateral ventricle transplantation of human amniotic epithelial cells显示文摘BACKGROUND: Human amniotic epithelial cells (HAECs) can differentiate into neurons, astrocytes,and oligodendrocytes. They biologically secrete many active neurotrophins and have the capacity tometabolize dopamine enzymes. These features underlie a theoretical basis for the treatment ofParkinson's disease (PD).OBJECTIVE: To investigate the survival and differentiation of transplanted HAECs in the lateralventricle of PD model rats, and to explore its effect on circling behavior, as well as levels ofdopamine (DA), the metabolite homovanillic acid, dihydroxyphenyl acetic acid,5-hydroxyindoleacetic acid, and 5-hydroxytryptamine in the striatum.DESIGN, TIME AND SETTING: A randomized, controlled, animal study was performed at theInstitute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences, ChineseAcademy of Sciences, and Shanghai Celstar Institute of Biotechnology from May 2007 to December2008.MATERIALS: HAECs were derived from the placental chorion following caesarean delivery at theShanghai International Maternal and Child Health Hospital. 6-hydroxydopamine (6-OHDA), andmouse anti-human Vimentin monoclonal antibody were purchased from Sigma, USA; mouseanti-human nestin and tyrosine hydroxylase (TH) monoclonal antibodies were purchased fromChemicon, USA.METHODS: A total of 114 healthy, adult, Sprague Dawley rats were randomly assigned to twogroups: PD model [n = 90, stereotactic microinjection of 2 μL 6-OHDA (3.5 μg/μL) into the striatum]and control (n = 24, no treatment). The 51 successful PD model rats were randomly divided into 3subgroups (n = 17): HAEC, PBS, and model. The HAEC and PBS groups were respectively injectedwith 10 μL PBS solution containing 1 x 105/mL HAECs or 10 μL PBS into the lateral ventricle. Themodel group was not treated.MAIN OUTCOME MEASURES: TH protein expression in the striatum was evaluated byimmunohistochemistry 5 weeks after HAEC transplantation. At 10 weeks, HAEC survival in thelateral ventricle was investigated by immunofluorescent staining; differentiation of HAECs in thelateral and third ventricles was examined by TH immunohistochemistry; concentrations of DA,homovanillic acid, dihydroxyphenyl acetic acid, 5-hydroxyindoleacetic acid, and 5-hydroxytryptaminein the striatum, as well as DA concentration in the cerebrospinal fluid, were measured withhigh-performance liquid chromatography-electrochemical detection. Circling behavior of PD modelrats was consecutively observed for 10 weeks following intraperitoneal injection of amphetamine 1week after successful model establishment.RESULTS: In the HAEC group, the number of TH-positive cells significantly increased in thestriatum, and circling behavior significantly decreased, compared with the PBS and model groups(P < 0.01). In addition, monoamine concentrations in the striatum, as well as DA concentrations inthe cerebrospinal fluid, significantly increased, compared with the PBS group (P < 0.05-0.01 ).Moreover, a large number of nestin-, vimentin-, and TH-positive cells were observed in the lateraland third ventricles following HAEC injection. CONCLUSION: HAECs survived for 10 weeks with no overgrowth following transplantation into thelateral ventricle of PD model rats. Moreover, the cells differentiated into dopaminergic neurons,which increased DA secretion. HAEC transplantation improved cycling behavior in PD model rats. | Shouru Xue Xinxin Yang Wanli Dong Guozhen Hui Lihe GUO | 2009 | Neural Regeneration Research2009，4，12： | 6 |
| 5 | Structural OH in mantle-derived clinopyroxene megacrysts from Nushan显示文摘Thirteen clinopyroxene megacrysts from Nushan Cenozoic basalts have been investigated by Fourier transform infrared spectrometer(FTIR). The results demonstrate that all samples contain trace amount of structural OH. Clinopyroxenes from Nushan show four groups of absorption bands, in which, 3 472-3 506 cm -1 is the major characteristic one. It is suggested that the position of major band is probably related to Al content: the position of major characteristic band for high_Al samples is around 3 470 cm -1 , whereas that for low_Al samples around 3 620 cm -1 . | Qunke Xia Daogong Chen Lihe Guo | 1998 | Chinese Science Bulletin1998，43，20： | 6 |
| 6 | Human amniotic epithelial cell feeder layers maintain mouse embryonic stem cell pluripotency via epigenetic regulation of the c-Myc promoter显示文摘老鼠胚胎的干细胞(转换字符) 在老鼠的 feeder 层上典型地是有教养的胚胎的成纤维细胞(MEF ) ,与白血病,禁止的因素(LIF ) 增加了处于一个无差别的状态维持他们。我们以前证明了人的羊膜的上皮的房间(hAECs ) 能被用作 feeder 房间维持老鼠转换字符 pluripotency,但是为这的机制是未知的。在现在的学习,我们发现那 CpG 5 c-Myc 基因仍然是的岛在在 hAECs 上有教养的老鼠转换字符的 hypomethylated。另外,在 c-Myc 基因倡导者 histone H3 的 acetylation 和 histone H3K4 的 trimethylation 铺平比在在 MEF 上有教养的 ES 房间的那些在在 hAECs 上有教养的 ES 房间是更高的。这些数据建议 hAECs 能经由 c-Myc 的 epigenetic 修正改变鼠标转换字符基因表示,提供为处于一个无差别的状态的转换字符的导致 hAEC 的维护的可能的机制。 | Te Liu Weiwei Cheng Tianjin Liu Lihe Guo Qin Huang Lizhen Jiang Xiling Du Fuhui Xu Zhixue Liu Dongmei Lai | 2010 | Acta Biochimica et Biophysica Sinica2010，42，2： | 6 |
| 7 | Amniotic fluid-derived stem cells mixed with platelet rich plasma for restoration of rat alveolar bone defect显示文摘从羊膜的液体孤立的干细胞作为房间治疗和织物工程的一个有希望的候选人被显示出。然而, maxillofacial 骨头织物工程的地里的羊膜的导出液体的干细胞(AFSC ) 的试验性、现出症状之前的潜的应用仍然是有限的。在这研究,老鼠 AFSC 成功地在 vitro 被收获并且描绘。老鼠 AFSC 显示出典型 fibroblastoid 形态学,稳定的增长活动和多区别潜力。Flow-cytometry 分析为象 CD34 和 CD45 那样的造血的标记证明这些房间为 CD29, CD44,和 CD90 是积极的,当否定时。与血小板富人一起的 AFSCs-premixed 的再生表演在牙槽的骨头缺点的恢复的血浆(PRP ) 胶化进一步用一只修改老鼠被调查颚的牙槽的缺点模型。微型电脑断层摄影术和组织学的检查在操作与控制组作比较以后在 4 和 8 个星期与 PRP 胶化显示出 AFSCs-premixed 的一个优异再生能力。而且,植入的 AFSC 能在缺点地点幸存并且直接参予骨头织物新生。一起拿,这些结果为牙槽的缺点恢复显示了基于 AFSCs 的牙槽的骨头织物工程策略的可行性。 | Minjiao Wang Hongliang Li Jiawen Si Jiewen Dai Jun Shi Xudong Wang Lihe Guo Guofang Shen | 2017 | Acta Biochimica et Biophysica Sinica2017，49，3： | 5 |
| 8 | Non-Oxidative Aromatization of CH4-C_3H_8 over La-Promoted Zn/HZSM-5 Catalysts显示文摘有在支持 La 的 Zn/HZSM-5 催化剂上的 C3H8 的混合 CH4 的非氧化的芳构化与空间速度 600 h 在 823 K 在一个改正床反应堆被学习(? 1 ) 并且 CH4/C3H8 (摩尔比)=5:1。丙烷变换和芳香的选择分别地在催化剂上直到 99% 和 60% ,当甲烷变换与 30% 的最高的变换有诱导期时。催化剂的结构和表面酸味被 XRD, NH3-TPD 和 TG-DTA 描绘。在这项活动和选择的反应和再生条件的影响也被调查。给词调音:芳构化;甲烷;丙烷;新生;镧;Zn; | Lihe Zheng Dong Xuan Jianjun Guo Hui Lou Xiaoming Zheng | 2006 | Journal of Natural Gas Chemistry2006，15，1： | 4 |
| 9 | Human amniotic epithelial cells express specific markers of nerve cells and migrate along the nerve fibers in the corpus callosum显示文摘Human amniotic epithelial cells were isolated from a piece of fresh amnion. Using immunocytochemical methods, we investigated the expression of neuronal phenotypes (microtubule-associated protein-2, glial fibrillary acidic protein and nestin) in human amniotic epithelial cells. The conditioned medium of human amniotic epithelial cells promoted the growth and proliferation of rat glial cells cultured in vitro, and this effect was dose-dependent. Human amniotic epithelial cells were further transplanted into the corpus striatum of healthy adult rats and the grafted cells could integrate with the host and migrate 1-2 mm along the nerve fibers in corpus callosum. Our experimental findings indicate that human amniotic epithelial cells may be a new kind of seed cells for use in neurograft. | Zhiyuan Wu Guozhen Hui Yi Lu Tianjin Liu Qin Huang Lihe Guo | 2012 | Neural Regeneration Research2012，7，1： | 3 |
| 10 | The Discovery of Magnesioferrite from Au (Fe, Cu) Magnesian Skarn Deposits and Study of the Magnesioferrite-Magnesiomagnetite Series显示文摘Magnesioferrite, a rare metasomatic mineral, was discovered for the first time in China from the Qinlou Au (Fe, Cu) magnesian skarn deposit, Sanpu, Huaibei, Auhui Province, and the Mulonggou Fe (Mo, Cu) magnesian skarn deposit, Luonan County, Shaanxi Province. In this paper, the geological setting, mineral associations, chemical composition, some physical properties, X-ray powder diffraction data and infrared spectroscopy of magnesioferrite and magnesiomagnetite are discussed. Magnesioferrite contains 17.66%-13.48% of MgO. Its main associated minerals are clinohumite, chondrodite, serpentine,, calcite and magnesiomagnetite. The density of magnesioferrite is 4.537-4.720, reflectances in percent are: 17.8-18.1, hardness is 838-900 kg/mm2, and the cell parameter a0 = 8.371-8.379 A. A systematic study of the magnesioferrite-magnesiomagnetite-magnetite series suggests that along with the increase of magnesioferrite molecules in the mineral, the density, reflectances and cell parameters decrease | ZHAO Yiming ZHANG Yinan BI Chengsi GUO Lihe | 1998 | Acta Geologica Sinica(English Edition)1998，72，4： | 3 |
| 11 | Synthesis and Anti-HIV Activity of a Series of 6-Modified 2',3'-Dideoxyguanosine and 2',3'-Didehydro-2',3'- dideoxyguanosine Analogs显示文摘寻找潜在的 2,3-dideoxyguanosine (ddG ) 和 2,3-didehydro-2,3-dideoxyguanosine (D4G ) prodrugs,一系列 6-modified ddG, D4G 类似物在基于房间的试金为他们的 anti-HIV 活动和 cytotoxities 被综合并且评估。所有类似物显示出低 cytotoxicities,他们中的一些显示了良性的 anti-HIV 活动。在 vivo, ddGTP 和 D4TTP 的活跃 triphosphate 形式,被一个新奇、灵巧的“一个壶”也综合方法。由 Taq, Therminater DNA 聚合酶和在 DNA/RNA 海滨合并的 HIV 反向的 transcriptase (RT ) 的 ddGTP 和 D4TTP 的识别被一个非放射性方法调查, Km 是坚定的。 | Lujia Xie Xiantao Yang Delin Pan Yingli Cao Mou Cao Guichun Lin Zhu Guan Ying Guo Lihe Zhang Zhenjun Yang | 2013 | Chinese Journal of Chemistry2013，31，9： | 2 |
| 12 | Nucleolin-targeting liposomes guided by aptamer AS1411 for the delivery of siRNA for the treatment of malignant melanomas显示文摘 | Liyu Li Jianjun Hou Xinjie Liu Yujia Guo Yun Wu Lihe Zhang Zhenjun Yang | 2014 | Biomaterials2014，，： | 1 |
| 13 | Low microRNA-199a expression in human amniotic epithelial cell feeder layers maintains human-induced pluripotent stem cell pluripotency via increased leukemia inhibitory factor expression显示文摘导致人的 pluripotent 茎(iPS ) 房间分享象胚胎的干细胞的一样的关键性质,并且可以从病人特定或疾病特定的来源被产生,它为个性化的药,药屏幕,或细胞的治疗使他们漂亮。处于一个无差别的自我更新的状态的正常 iPS 房间的长期的耕作和维护是主要挑战。我们的以前的研究证明了人的羊膜的上皮的房间(HuAECs ) 能为老鼠和人提供 feeder 房间的好来源胚胎的干细胞,或 spermatogonial 干细胞,他们表示内长的白血病禁止的因素(LIF ) 在高级。这里,我们在 HuAECs 在内长的 LIF 表示上检验了外长的 microRNA-199a 规定的效果,并且接着在人的 iPS 房间 pluripotency 上。我们发现有 microRNA-199a 异种的 HuAECs feeder 房间 transfected 在高水平表示了 LIF,允许 iPS 维持在长期的文化的碱的磷酸酶活动和在严重联合 immunodeficient 老鼠的形式 teratomas 的高水平。干细胞标记的表达式与 microRNA-199a 异种在在 HuAECs feeder 房间 transfected 上有教养的 iPS 被增加,与在有 microRNA-199a 或鼠标胚胎成纤维细胞的 HuAECs transfected 上有教养的 iPS 相比。一起拿,这些结果建议那 LIF 表情可能被 microRNA-199a,和 LIF 调整是在 feeder 房间的一个关键部件,并且也为人的 iPS 房间的维护被要求在一无差别,能够自强的 proliferative 状态。 | Te Liu Qing Chen Yongyi Huang Qin Huang Lizhen Jiang Lihe Guo | 2012 | Acta Biochimica et Biophysica Sinica2012，44，3： | 1 |
| 14 | Water in Mantle-derived Xenoliths in the Hannuoba Basalt显示文摘Minerals of various mantle-derived xenoliths from the Hannuoba basalt in Hebei Province have been studied by means of IR spectroscopy. The results show that all xenoliths from the mantle at depths <75 km contain trace amounts of water (0.45%-11.6×10-2 % H2O). The data of about 0.1% H2O contained in primary pyrolite estimated by earlier studies may be on the high side. The water might enter the frameworks of olivine, pyroxene and garnet earlier than it entered those of amphibole and phlogopite. The presence of water in amphibole and phlogopite may be a local phenomenon of water enrichment, which is related to relatively small-scale magmatic or metasomatic events although they can contain a hundred times more water than pyroxene contains. There is a little more water (1.11%-3.01×10-2 % of H2O mostly) in xenoliths from the Hannuoba basalt than in those from mid-ocean ridge basalt and kimberlites of South Africa (less than 1×10-2 % of H2O mostly). This indicates the heterogeneity of water in time and | GUO Lihe LIN Xingyuan XIE Manze FENG Jialin WU Shuqi | 1998 | Acta Geologica Sinica(English Edition)1998，72，2： | 1 |
| 15 | CD44+/CD105+ HumanAmniotic Fluid Mesenchymal Stem Cells Survive and Prolifer-ate in the Ovary Long-Term in a Mouse Model of Chemothera-py-Induced Premature Ovarian Failure显示文摘 | Te Liu Yongyi Huang Lihe Guo | 2012 | Int J Med 5d2012，9，7： | 1 |
| 16 | Existence of an Endogenous Glutamate and Aspartate Transporter in Chinese Hamster Ovary Cells显示文摘中国仓鼠卵巢房间出现内长高亲密关系 Na+ 依赖充斥使挫折运输活动。这项运输活动是类似于战略上在中央神经系统表示的一个 glut 使挫折 transporter 家庭的运动联盟者并且是 pharmacologically 不同的充斥使挫折 transporter-1 或有刺激性的氨基酸搬运人 1。象 transporter 一样的 glutamate/aspartate transporter (GLAST ) 的 cDNA 被获得并且分析。推出的氨基酸顺序高证明到人,老鼠,和老鼠 GLAST.We 的类似断定象 GLAST 一样充斥使挫折 transporter 在可能交谈的中国仓鼠卵巢房间存在内长高亲密关系 Na+ 依赖充斥在这些房间明显的使挫折运输活动。 | Xunhe JI Yuhua JIN Yaoyue CHEN Chongyong LI Lihe GUO | 2007 | Acta Biochimica et Biophysica Sinica2007，39，11： | 1 |
| 17 | Characterization of a micro-roughened TiO2/ZrO2 coating: mechanical properties and HBMSC responses in vitro显示文摘 | Jiawen Si Jianjun Zhang Sha Liu Wenbin Zhang Dedong Yu Kudong Wang Lihe Guo Steve G.F. Shen | 2014 | Acta Biochimica et Biophysica Sinica2014，46，7： | 1 |
| 18 | Identification of Ser^(354) and Ser^(357) involved in the function of norepinephrine transporter显示文摘Conserved Ser 354, Ser 357 in the seventh transmembrane domain of NET were mutated and the function of mutants was studied by 3H-NE influx measurement. Double mutation at these two serine sites reduced the activity of NET to base level. It is inferred that these two sites may be involved in the recognition and transport activity of the NET protein. | HUANG Fang 1, LIU Yanhong 1, FEI Jian 1*, GUO Lihe 1* and Wolfgang Schwarz 2 1. Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China 2. Max-Planck Institute for Biophysik, Kennedy Allee 70, 60956 Frankfurt/Mai | 1998 | Chinese Science Bulletin1998，43，18： | 0 |
| 19 | Studies of substrate binding region of mEAAC1 and mASCT1 by constructing chimeras显示文摘The cDNA chimeras between two subtypes of mouse excitatory amino acid transporter family, mouse excitatory amino acid carrier 1 (mEAAC1) and mouse alanine serine cysteine transporter 1 (mASCT1), were constructed by recombinant PCR. After transcription in vitro, the cRNA was injected and expressed in Xenopus laevis oocytes. 3H-Glu and 3H-Ser were used as isotopic tracer to measure the flux of amino acids. The results showed that there might not be the key amino acids responsible for substractive specificity in the NH2-terminal and its adjacent regions of these two transporters, which probably supported the formation of the substrata binding sites. | Jing Li Jibin Peng Jian Fei Fang Huang Quanbao Gu Lihe Guo | 1999 | Chinese Science Bulletin1999，44，6： | 0 |
| 20 | Tribological properties of PTFE-based fabric composites at cryogenic temperature显示文摘Fabric composites are widely employed in self-lubricating bearing liners as solid lubrication materials.Although the tribological behaviors of fabric composites have been extensively studied,the cryogenic tribological properties and mechanisms have been scarcely reported and are largely unclear to instruct material design for aerospace and other high-tech applications.Herein,the tribological properties of polytetrafluoroethylene(PTFE)-based hybrid-fabric composites were investigated at cryogenic and ambient temperatures in the form of pin-on-disk friction under heavy loads.The results suggest that the friction coefficients of the hybrid-fabric composites obviously increase with a decrease in wear when the temperature drops from 25 to−150°C.Moreover,thermoplastic polyetherimide(PEI),as an adhesive for fabric composites,has better cryogenic lubrication performance than thermosetting phenol formaldehyde(PF)resin,which can be attributed to the flexible chemical structure of PEI.The excellent lubrication performance of hybrid-fabric composites is attributed to the transfer film formed by PTFE fibers on the surface of fabrics. | Mingkun XU Zidan WANG Lihe GUO Liming TAO Tianbao MA Tingmei WANG Qihua WANG | 2024 | Friction2024，12，2： | 0 |
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