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| 1 | Partial Beclin 1 silencing aggravates doxorubicin-and Fasinduced apoptosis in HepG2 cells显示文摘瞄准:在 HepG2 房间的危险性上调查 Beclin 1 的角色在反船边交货抗体或 doxorubicin 治疗以后经历 apoptosis。方法:Beclin 1 silencing 用 RNA 干扰被完成。DNA 倍性, apoptotic 房间的百分比和 mitochondrial 膜电位被流动血细胞计数估计。多形核白细胞(自动数据处理核糖) 的 Beclin 1, Bcl-X (L) 和细胞色素 c,和劈开聚合酶(PARP ) 的层次是由使用西方的污点的 assayed。结果:Beclin 1 表示在 Beclin 1 siRNA transfection 以后由 75% 72 h 减少了。部分 Beclin 1 silencing 显著地增加了 subG1 房间的百分比有 doxorubicin 或反船边交货抗体的 24 和 40 h 术后疗法,分别地并且这 potentiation 被处理与一个 pan-caspase 禁止者废除。部分 Beclin 1 silencing 也增加了 PARP 劈开, mitochondrial 膜去极和 cytosolic 细胞色素 c。部分 Beclin 1 silencing 的 pro-apoptotic 后果没在 Bcl-X (L) 表示与衰落被联系。结论:部分 Beclin 1 silencing 在与反船边交货抗体或与 doxorubicin 对待的 HepG2 房间加重 mitochondrial permeabilization 和 apoptosis。 | Fanny Daniel Agnès Legrand Dominique Pessayre Nathalie Vadrot Véronique Descatoire Dominique Bernuau | 2006 | World Journal of Gastroenterology2006，12，18： | 11 |
| 2 | c-Fos overexpression increases the proliferation of human hepatocytes by stabilizing nuclear Cyclin D1显示文摘AIM: To investigate the effect of stable c-Fos overexpression on immortalized human hepatocyte (IHH) proliferation. METHODS: IHHs stably transfected with c-Fos (IHH-Fos) or an empty vector (IHH-C) were grown in medium supplemented with 1% serum or stimulated with 10% serum. Cell proliferation was assessed by cell counts, 3H-thymidine uptake and fl ow cytometry analyses. The levels of cell cycle regulatory proteins (Cyclin D1, E, A) cyclin dependent kinases (cdk) cdk2, cdk4, cdk6, and their inhibitors p15, p16, p21, p27, to- tal and phosphorylated GSK-3β and epidermal growth factor receptor (EGF-R) were assayed by Western blot- ting. Analysis of Cyclin D1 mRNA levels was performed by reverse transcription-polymerase chain reaction and real-time polymerase chain reaction (PCR) analysis. Stability of Cyclin D1 was studied by cycloheximide blockade experiments. RESULTS: Stable c-Fos overexpression increased cell proliferation under low serum conditions and resulted in a two-fold increase in [3H]-thymidine incorpora- tion following serum addition. Cell cycle analysis by fl ow cytometry showed that c-Fos accelerated the cell cycle kinetics. Following serum stimulation, Cyclin D1 was more abundantly expressed in c-Fos overexpressing cells. Cyclin D1 accumulation did not result from increased transcriptional activation, but from nuclear stabilization. Overexpression of c-Fos correlated with higher nuclear levels of inactive phosphorylated GSK- 3β, a kinase involved in Cyclin D1 degradation and higher levels of EGF-R mRNA, and EGF-R protein compared to IHH-C both in serum starved, and in serum stimulated cells. Abrogation of EGF-R signalling in IHH- Fos by treatment with AG1478, a specif ic EGF-R tyrosine kinase inhibitor, prevented the phosphorylation of GSK-3β induced by serum stimulation and decreased Cyclin D1 stability in the nucleus. CONCLUSION: Our results clearly indicate a positive role for c-Fos in cell cycle regulation in hepatocytes. Importantly, we delineate a new mechanism by which c-Fos could contribute to hepatocarcinogenesis through stabilization of Cyclin D1 within the nucleus, evoking a new feature to c-Fos implication in hepatocellular carcinoma. | Meryem Güller Kahina Toualbi-Abed Agnès Legrand Laurence Michel Alain Mauviel Dominique Bernuau Fanny Daniel | 2008 | World Journal of Gastroenterology2008，14，41： | 6 |
| 3 | Use of solid phase microextraetion eoupled with gas ehromatography for the determination of residual solvents in pharmaeeutieal produets显示文摘 | Legrand S Dugay J Vial J | 2003 | Journal of Chromatography A2003，999，： | 1 |
| 4 | Determination of ketamine and norketamine in plasma by micro-liquid chromatography-mass spectrometry显示文摘 | LEGRAND T ROY S MONCHAUD C | 2008 | Journal of Pharmaceutical and Biomedical Analysis2008，48，1： | 1 |
| 5 | Efficacy of hand rubbing with alcohol based solute on versus standard handwashing with antiseptic soap:Randomized clinical trial显示文摘 | Girou E Loyeau S Legrand P | 2002 | BMJ2002，325，7360： | 1 |
| 6 | Simultaneous determination of three carbapenem antibiotics in plasma by HPLC with ultraviolet detection显示文摘 | Legrand T Chhun S Rey E | 2008 | J Chromatogr B Analyt Technol Biomed Life Sci2008，875，2： | 1 |
| 7 | Efficacy of spectinomycin against mycoplasma boris induced pneumonia in conventionally reared Calves 显示文摘 | POUMARAT F LEGRAND D PHILIPPE S | 2001 | Vet Microbiol2001，80，： | 1 |
| 8 | Electrical Characterization of SrTiO3 Thin Films Deposited on Si(001) Substrate by Liquid Injection MOCVD显示文摘 | J Legrand S Lhostis Y Chang | 2004 | Microelectronic Engineering2004，72，： | 1 |
| 9 | Thermal impact of SaharanDust over land,part I:simulation显示文摘 | Cautenet G Legrand M Cautenet S | 1992 | Meteorology1992，31，： | 1 |
| 10 | Modeling of a rotor speed transient response with' radial rubbing显示文摘 | Roques S Legrand M | | 0，，05： | 1 |
| 11 | Factors driving extreme sexual size dimorphism of a sit-and-wait predator under low density显示文摘 | Legrand R S Morse D H | 2000 | Biological Journal of the Linnean Society2000，71，： | 1 |
| 12 | Modeling of a rotor speed transient response with radial rubbing显示文摘 | Roques S Legrand M Cartraud P | 2010 | Journal of Sound and Vibration2010，329，5： | 1 |
| 13 | Archi- tecture-controlled ' SMART ' ealix arene self-assem- blies in aqeous solution 显示文摘 | HOUMADI S LEGRAND L REINAUD O | 2007 | Langmuir2007，23，9： | 1 |
| 14 | Efficacy of handrubbing with alcohol based solution versus standard handwashing with antiseptic soap:randomised clinical trial显示文摘 | Girou E Loyeau S Legrand P | 2002 | BMJ2002，325，7360： | 1 |
| 15 | Factors driving extreme sexual size dimorphism of a sit-and-wait predator under low density显示文摘 | Legrand R S Morse D H | 2000 | Biological Journal of the Linnean Society2000，71，： | 1 |
| 16 | Errors in opioid prescribing: a prospective survey in cancer pain 显示文摘 | Shaheen P E Legrand S B Walsh D | 2010 | JPain Symptom Manage2010，39，4： | 1 |
| 17 | Development of three multiplex RT-PCR assays for the detection of 12 respiratory RNA viruses显示文摘 | Bellau-Pujol S Vabret A Legrand L | | 0，，1： | 1 |
| 18 | Manifes ted Flatness Predictions in Thin Strip Cold Rolling 显示文摘 | Abdelkhalek S Montmitonnet P Legrand N | 2008 | International Journal of Material Forming2008，，1： | 1 |
| 19 | Cutaneous infections in bullous pemphigoid patients treated with topical corticosteroids 显示文摘 | Boughrara Z Ingen-Housz-Oro S Legrand P | 2010 | Ann Dermatol Venereol2010，137，5： | 1 |
| 20 | Investigation of the interaction of velocity and concentration fields by coupling LDV to conductivity probe in a continuous stirred tank reactor (CSTR)显示文摘 | Benayad S Salem A Legrand J | 2001 | Chemical Engineering Journal2001，84，3： | 1 |
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