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| 1 | Service-aware 6G:An intelligent and open network based on the convergence of communication,computing and caching显示文摘With the proliferation of the Internet of Things(IoT),various services are emerging with totally different features and requirements,which cannot be supported by the current fifth generation of mobile cellular networks(5G).The future sixth generation of mobile cellular networks(6G)is expected to have the capability to support new and unknown services with changing requirements.Hence,in addition to enhancing its capability by 10–100 times compared with 5G,6G should also be intelligent and open to adapt to the ever-changing services in the IoT,which requires a convergence of Communication,Computing and Caching(3C).Based on the analysis of the requirements of new services for 6G,this paper identifies key enabling technologies for an intelligent and open 6G network,all featured with 3C convergence.These technologies cover fundamental and emerging topics,including 3C-based spectrum management,radio channel construction,delay-aware transmission,wireless distributed computing,and network self-evolution.From the detailed analysis of these 3C-based technologies presented in this paper,we can see that although they are promising to enable an intelligent and open 6G,more efforts are needed to realize the expected 6G network. | Yiqing Zhou Ling Liu Lu Wang Ning Hui Xinyu Cui Jie Wu Yan Peng Yanli Qi Chengwen Xing | 2020 | Digital Communications and Networks2020，6，3： | 57 |
| 2 | Analysis of DNA methylation in different maize tissues显示文摘DNA methylation plays an important role in gene expression regulation during biological development and tissue differentiation in plants. This study adopted methylation-sensitive Amplified fragment length polymorphism (AFLP) to compare the levels of DNA cyto- sine methylation at CCGG sites in tassel, bracteal leaf, and ear leaf from maize inbred lines, 18 White and 18 Red, respectively, and also examined specific methylation patterns of the three tissues. Significant differences in cytosine methylation level among the three tissues and the same changing tendency in two inbred lines were detected. Both MSAP (methylation sensitive amplification polymorphism) ratio and full methylation level were the highest in bracteal leaf, and the lowest in tassel. Meanwhile, different methylation levels were ob- served in the same tissue from the inbred lines, 18 White and 18 Red. Full methylation of internal cytosine was the dominant type in the maize genome. The differential methylation patterns in the three tissues were observed. In addition, sequencing of nine differentially me- thylated fragments and the subsequent blast search revealed that the cytosine methylated 5′-CCGG-3′sequences were distributed in re- peating sequences, in the coding and noncoding regions. Southern hybridization was used to verify the methylation polymorphism. These results clearly demonstrated the power of the MSAP technique for large-scale DNA methylation detection in the maize genome, and the complexity of DNA methylation change during plant growth and development. The different methylation levels may be related to specific gene expression in various tissues. | Yanli Lu, Tingzhao Rong, Moju Cao Maize Research Institute of Sichuan Agriculture University/Key Laboratory of Crop Genetic Resource and Improvement, Ministry of Education, Ya’an, 625014, China | 2008 | Journal of Genetics and Genomics2008，35，1： | 27 |
| 3 | The Genetic Architecture of Flowering Time and Photoperiod Sensitivity in Maize as Revealed by QTL Review and Meta Analysis显示文摘The control of flowering is not only important for reproduction,but also plays a key role in the processes of domestication and adaptation.To reveal the genetic architecture for flowering time and photoperiod sensitivity,a comprehensive evaluation of the relevant literature was performed and followed by meta analysis.A total of 25 synthetic consensus quantitative trait loci(QTL)and four hot-spot genomic regions were identified for photoperiod sensitivity including 11 genes related to photoperiod response or flower morphogenesis and development.Besides,a comparative analysis of the QTL for flowering time and photoperiod sensitivity highlighted the regions containing shared and unique QTL for the two traits.Candidate genes associated with maize flowering were identified through integrated analysis of the homologous genes for flowering time in plants and the consensus QTL regions for photoperiod sensitivity in maize(Zea mays L.).Our results suggest that the combination of literature review,meta-analysis and homologous blast is an efficient approach to identify new candidate genes and create a global view of the genetic architecture for maize photoperiodic flowering.Sequences of candidate genes can be used to develop molecular markers for various models of marker-assisted selection,such as marker-assisted recurrent selection and genomic selection that can contribute significantly to crop environmental adaptation. | Jie Xu Yaxi Liu Jian Liu Moju Cao Jing Wang Hai Lan Yunbi Xu Yanli Lu Guangtang Pan Tingzhao Rong | 2012 | Journal of Integrative Plant Biology2012，54，6： | 14 |
| 4 | Osthole inhibits proliferation of human breast cancer cells by inducing cell cycle arrest and apoptosis显示文摘Recent studies have revealed that osthole,an active constituent isolated from the fruit of Cnidium monnieri(L.) Cusson,a traditional Chinese medicine,possesses anticancer activity.However,its effect on breast cancer cells so far has not been elucidated clearly.In the present study,we evaluated the effects of osthole on the proliferation,cell cycle and apoptosis of human breast cancer cells MDA-MB 435.We demonstrated that osthole is effective in inhibiting the proliferation of MDA-MB 435 cells,The mitochondrion-mediated apoptotic pathway was involved in apoptosis induced by osthole,as indicated by activation of caspase-9 and caspase-3 followed by PARP degradation.The mechanism underlying its effect on the induction of G1 phase arrest was due to the up-regulation of p53 and p21 and down-regulation of Cdk2 and cyclin D1 expression.Were observed taken together,these findings suggest that the anticancer efficacy of osthole is mediated via induction of cell cycle arrest and apoptosis in human breast cancer cells and osthole may be a potential chemotherapeutic agent against human breast cancer. | Lintao Wang Yanyan Peng Kaikai Shi Haixiao Wang Jianlei Lu Yanli Li Changyan Ma | 2015 | The Journal of Biomedical Research2015，29，2： | 12 |
| 5 | Status and development of high-power laser facilities at the NLHPLP显示文摘In this paper, we review the status of the multifunctional experimental platform at the National Laboratory of High Power Laser and Physics(NLHPLP). The platform, including the SG-II laser facility, SG-II 9th beam, SG-II upgrade(SG-II UP) facility, and SG-II 5 PW facility, is operational and available for interested scientists studying inertial confinement fusion(ICF) and a broad range of high-energy-density physics. These facilities can provide important experimental capabilities by combining different pulse widths of nanosecond, picosecond, and femtosecond scales. In addition, the SG-II UP facility, consisting of a single petawatt system and an eight-beam nanosecond system, is introduced including several laser technologies that have been developed to ensure the performance of the facility. Recent developments of the SG-II 5 PW facility are also presented. | Jianqiang Zhu Jian Zhu Xuechun Li Baoqiang Zhu Weixin Ma Xingqiang Lu Wei Fan Zhigang Liu Shenlei Zhou Guang Xu Guowen Zhang Xinglong Xie Lin Yang Jiangfeng Wang Xiaoping Ouyang Li Wang Dawei Li Pengqian Yang Quantang Fan Mingying Sun Chong Liu Dean Liu Yanli Zhang Hua Tao Meizhi Sun Ping Zhu Bingyan Wang Zhaoyang Jiao Lei Ren Daizhong Liu Xiang Jiao Hongbiao Huang Zunqi Lin | 2018 | High Power Laser Science and Engineering2018，6，4： | 8 |
| 6 | Embryonic endothelial evolution towards first hematopoietic stem cells revealed by single-cell transcriptomic and functional analyses显示文摘Hematopoietic stem cells(HSCs)in adults are believed to be born from hemogenic endothelial cells(HECs)in mid-gestational embryos.Due to the rare and transient nature,the HSC-competent HECs have never been stringently identified and accurately captured,let alone their genuine vascular precursors.Here,we first used high-precision single-cell transcriptomics to unbiasedly examine the relevant EC populations at continuous developmental stages with intervals of 0.5 days from embryonic day(E)9.5 to E11.0.As a consequence,we transcriptomically identified two molecularly different arterial EC populations and putative HSCprimed HECs,whose number peaked at El 0.0 and sharply decreased thereafter,in the dorsal aorta of the aorta-gonadmesonephros(AGM)region.Combining computational prediction and in vivo functional validation,we precisely captured HSCcompetent HECs by the newly constructed Neurl3-EGFP reporter mouse model,and realized the enrichment further by a combination of surface markers(Procr+Kit+CD44+,PK44).Surprisingly,the endothelial-hematopoietic dual potential was rarely but reliably witnessed in the cultures of single HECs.Noteworthy,primitive vascular ECs from E8.0 experienced two-step fate choices to become HSC-primed HECs,namely an Initial arterial fate choice followed by a hemogenic fate conversion.This finding resolves several previously observed contradictions.Taken together,comprehensive understanding of endothelial evolutions and molecular programs underlying HSC-primed HEC specification in vivo will facilitate future investigations directing HSC production in vitro. | Siyuan Hou Zongcheng Li Xiaona Zheng Yun Gao Ji Dong Yanli Ni Xiaobo Wang Yunqiao Li Xiaochen Ding Zhilin Chang Shuaili Li Yuqiong Hu Xiaoying Fan Yu Hou Lu Wen Bing Liu Fuchou Tang Yu Lan | 2020 | Cell Research2020，30，5： | 8 |
| 7 | Surface quality of cold rolling aluminum strips under lubrication condition显示文摘The effects of oil film on the rolled surface,including surface roughness and topography,were investigated during cold rolling of aluminum strips.Various mineral oils with viscosities from 0.10 to 1.6 Pa.s were used to obtain different oil film thick- nesses.Results from experiment and calculation show that the thicker oil film protects the initial roughening surface so that it leads to an increase in roughness of the rolled surface,in particular when the surface roughness has the character of direction.The rolled surface roughness was determined byλ,which is the ratio of oil film thickness to the combined surface roughness.Whenλ>3,the rolled surface roughness increases rapidly with the increase in oil viscosity,whereas the surface roughening has already occurred whenλ<3,but the increase of the rolled surface roughness with increasing viscosity is not distinct. | Jianlin Sun Wang Lu Yanli Ma Qingbing Shi Aihua Zhang Jian Li | 2008 | Journal of University of Science and Technology Beijing2008，15，3： | 6 |
| 8 | Soil Macropore Structure Characterized by X-Ray Computed Tomography Under Different Land Uses in the Qinghai Lake Watershed, Qinghai-Tibet Plateau显示文摘Quantification of soil macropores is important to enhance our understanding of preferential pathways for water, air, and chemical movement in soils. However, the soil architecture of different land uses is not well understood in elusive alpine regions. The objective of this study was to quantify the architecture of soil macropores in a Kobresia meadow, farmland, and sand in the Qinghai Lake watershed of northeastern Qinghai-Tibet Plateau, China using X-ray computed tomography. Nine soil cores at 0–50 cm depth were collected at three sites with three replicates. At each site, the three collected cores were scanned using a GE Hi Speed FX/i medical scanner(General Electric, USA). To analyze soil architecture, the number of macropores, macroporosity, and mean macropore equivalent diameter within the 50 cm soil profile were determined from the X-ray computed tomography. Analysis of variance indicated that land use significantly influenced macroporosity, mean macropore equivalent diameter, and number of macropores. The soils of the Kobresia meadow and farmland had greater macroporosity and developed deeper and longer macropores than that of sand. For the Kobresia meadow, macropores were distributed mainly in the 0–10 cm soil layer, while they were distributed in the 0–20 cm soil layer for the farmland. The large number of macropores observed in the soils of the Kobresia meadow and farmland could be attributed to greater root development. The results of this study provided improved quantitative evaluation of a suite of soil macropore features with significant implications for non-equilibrium flow prediction and chemical transport modeling in soils. | HU Xia LI Zongchao LI Xiaoyan WANG Pei ZHAO Yunduo LIU Lianyou LU Yanli | 2018 | Pedosphere2018，28，3： | 3 |
| 9 | Reconstructing the annual precipitation variation since 1899 based on tree-ring width in the western Hedong sandy land of Ningxia显示文摘Based on the analysis of the correlation between the tree-ring width of Pinus tabulaeformis and the climate factors in the western Hedong sandy land of Ningxia, a conversion equation between the annual precipitation and the tree-ring width since 1899 was reconstructed. The results of cross verification indicated that the conversion equation is stable and the reconstructed results are reliable. The result of reconstructed annual precipitation showed the remarkable fluctuation of precipitation and dry-to-wet variation before the 1940s. The smaller fluctuation and high frequent changes of precipitation occurred during the period of 1940s-1980s and after the 1980s the change trend of the precipitation became high periodic extent and low frequent. The study found that there were some coincidences with the climate change in Changling Mountains, Helan Mountains and the east of Qilian Mountains. The relatively dry periods in the beginning of 20th century, 1920s to 1930s, the end of the 20th century and 2004 to 2006 in the western Hedong sandy land of Ningxia accelerated the desertification, while the relatively humid period during the periods of the 1910s-1920s, 1930s-1940s and 1990s is favorable to prevent and control the desertification, and to weaken the climate warming and drying. The periods of annual precipitation variation in the western Hedong sandy land of Ningxia since 1899 are approximately 2-4 years, 5-7 years and 10 years. | Wang, YaJun Gao, ShangYu Ma, YuZhen Lu, RuiJie Sang, YanLi Meng, HongWei | 2010 | Journal of Arid Land2010，2，4： | 3 |
| 10 | Genome-level diversification of eight ancient tea populations in the Guizhou and Yunnan regions identifies candidate genes for core agronomic traits显示文摘The ancient tea plant,as a precious natural resource and source of tea plant genetic diversity,is of great value for studying the evolutionary mechanism,diversification,and domestication of plants.The overall genetic diversity among ancient tea plants and the genetic changes that occurred during natural selection remain poorly understood.Here,we report the genome resequencing of eight different groups consisting of 120 ancient tea plants:six groups from Guizhou Province and two groups from Yunnan Province.Based on the 8,082,370 identified high-quality SNPs,we constructed phylogenetic relationships,assessed population structure,and performed genome-wide association studies(GWAS).Our phylogenetic analysis showed that the 120 ancient tea plants were mainly clustered into three groups and five single branches,which is consistent with the results of principal component analysis(PCA).Ancient tea plants were further divided into seven subpopulations based on genetic structure analysis.Moreover,it was found that the variation in ancient tea plants was not reduced by pressure from the external natural environment or artificial breeding(nonsynonymous/synonymous=1.05).By integrating GWAS,selection signals,and gene function prediction,four candidate genes were significantly associated with three leaf traits,and two candidate genes were significantly associated with plant type.These candidate genes can be used for further functional characterization and genetic improvement of tea plants. | Litang Lu Hufang Chen Xiaojing Wang Yichen Zhao Xinzhuan Yao Biao Xiong Yanli Deng Degang Zhao | 2021 | Horticulture Research2021，8，1： | 3 |
| 11 | New algorithms for verifiable outsourcing of bilinear pairings显示文摘Dear editor,Outsourcing of computation has received widespread attention with the development of cloud computing and the proliferation of mobile devices[1].Despite the huge benefits that it provides,it also encounters some security concerns and other challenges.First,the | Yanli REN Ning DING Tianyin WANG Haining LU Dawu GU | 2016 | Science China(Information Sciences)2016，59，9： | 3 |
| 12 | Microscopic phase field study of the antisite defect of Ni_3 Al in binary Ni-Al alloys显示文摘The temporal evolution feature of a microscopic phase field model is utilized to study the antisite defects of L1 2-Ni 3 Al;this is quite different from other physicist’ interests.There are mainly two points in brief.Firstly,antisite defects Ni Al and Al Ni ,which are caused by the deviation from the stoichiometric Ni 3 Al,coexist in the Ni 3 Al phase.The surplus Ni atom in the Ni-rich side is prone to substitute Al thus producing the antisite defect Ni Al that maintains the stability of the L1 2 structure.In other case,the surplus Al atom in the Al-rich side is accommodated by a Ni sublattice consequently giving rise to antisite defect Al Ni .The calculated equilibrium occupancy probability of Ni Al is much higher than that of Al Ni .This point is generally in line with other theoretical and experimental works.Additionally,both Ni Al and Al Ni have a strong negative correlation to time step during the disorder-order transformation.Since the initial value of Ni Al and Al Ni on each site of the matrix is right at the concentration that we set,we can observe the decrease process of Ni Al and Al Ni from the initial disordered high anti-structure state to their respective equilibrium state,i.e.to the result of the ordering process further coarsening. | ZHANG Jing ,CHEN Zheng,LU YanLi,ZHANG MingYi & WANG YongXin State Key Laboratory of Solidification Processing School of Material Science and Engineering,Northwestern Polytechnical University,Xi’an 710072,China | 2010 | Science China(Physics,Mechanics & Astronomy)2010，53，11： | 3 |
| 13 | Water Vapor Motion Signal Extraction from FY-2E Longwave Infrared Window Images for Cloud-free Regions:The Temporal Difference Technique显示文摘The aim of this study is to calculate the low-level atmospheric motion vectors(AMVs) in clear areas with FY-2E IR2window(11.59–12.79 μm) channel imagery, where the traditional cloud motion wind technique fails. A new tracer selection procedure, which we call the temporal difference technique, is demonstrated in this paper. This technique makes it possible to infer low-level wind by tracking features in the moisture pattern that appear as brightness temperature(TB) differences between consecutive sequences of 30-min-interval FY-2E IR2 images over cloud-free regions. The TBdifference corresponding to a 10% change in water vapor density is computed with the Moderate Resolution Atmospheric Transmission(MODTRAN4)radiative transfer model. The total contribution from each of the 10 layers is analyzed under four typical atmospheric conditions: tropical, midlatitude summer, U.S. standard, and midlatitude winter. The peak level of the water vapor weighting function for the four typical atmospheres is assigned as a specific height to the TB'wind'. This technique is valid over cloudfree ocean areas. The proposed algorithm exhibits encouraging statistical results in terms of vector difference(VD), speed bias(BIAS), mean vector difference(MVD), standard deviation(SD), and root-mean-square error(RMSE), when compared with the wind field of NCEP reanalysis data and rawinsonde observations. | YANG Lu WANG Zhenhui CHU Yanli ZHAO Hang TANG Min | 2014 | Advances in Atmospheric Sciences2014，31，6： | 3 |
| 14 | Revisiting the role of delta-1-pyrroline-5-carboxylate synthetase in drought-tolerant crop breeding显示文摘The delta-1-pyrroline-5-carboxylate synthetase(P5CS)gene exercises a protective function in stressed plants.However,the relationship between proline accumulation caused by P5CS and abiotic stress tolerance in plants is not always clear,as P5CS overexpression has been reported to repress plant growth under normal conditions in several reports.We re-evaluated the role of P5CS in drought-tolerant rice breeding by expressing the AtP5CS1 and feedback-inhibition-removed AtP5CS1(AtP5CS1^(F128A))genes under the regulation of an ABA-inducible promoter to avoid the potential side effects of P5CS overexpression under normal conditions.ABA-inducible AtP5CS1 and AtP5CS1^(F128A) increased seedling growth in a nutrient solution(under osmotic stress)and grain yield in pot plants.However,the evidently deleterious effects of AtP5CS1 on grain quality,tiller number,and grain yield in the field indicated the unsuitability of P5CS for drought-tolerance breeding. | Xuanjun Feng Yue Hu Weixiao Zhang Rongqian Xie Huarui Guan Hao Xiong Li Jia Xuemei Zhang Hanmei Zhou Dan Zheng Ying Wen Qingjun Wang Fengkai Wu Jie Xu Yanli Lu | 2022 | The Crop Journal2022，10，4： | 2 |
| 15 | Heterogeneity in endothelial cells and widespread venous arterialization during early vascular development in mammals显示文摘Arteriogenesis rather than unspecialized capillary expansion is critical for restoring effective circulation to compromised tissues in patients.Deciphering the origin and specification of arterial endothelial cells during embryonic development will shed light on the understanding of adult arteriogenesis.However,during early embryonic angiogenesis,the process of endothelial diversification and molecular events underlying arteriovenous fate settling remain largely unresolved in mammals.Here,we constructed the single-cell transcriptomic Iandscape of vascular endothelial cells(VECs)during the time window for the occurrenee of key vasculogenic and angiogenic events in both mouse and human embryos.We uncovered two distinct arterial VEC types,the major artery VECs and arterial plexus VECs,and un expectedly diverge nt arteriove nous characteristics among VECs that are located in morphologically undistinguishable vascular plexus intra-embryonically.Using computational prediction and further lineage tracing of venous-featured VECs with a newly developed Nr2f2^(CrexER) | Siyuan Hou Zongcheng Li Ji Dong Yun Gao Zhilin Chang Xiaochen Ding Shuaili Li Yunqiao Li Yang Zeng Qian Xin Baihan Wang Yanli Ni Xiaowei Ning Yuqiong Hu Xiaoying Fan Yu Hou Xianlong Li Lu Wen Bin Zhou Bing Liu Fuchou Tang Yu Lan | 2022 | Cell Research2022，32，4： | 2 |
| 16 | Non-mechanical behaviors of soft clay in two-dimensional electro-osmotic consolidation显示文摘To investigate the soil behaviors in a direct current field on both spatial and temporal scales, a 1: 5 scale model test was conducted in laboratory to simulate the two-dimensional (2D) electro-osmotic consolidation of soft clay foundation. Volume of drainage, intensity, voltage, water content and pH value of water collected in the cathodes were monitored. The pH values of soil and the mass of anodes were measured before and after the test. The test results indicate that the unsaturated state, resultant from fissures induced by the differences in water contents, is favorable to dynamic compaction of soil during electro-osmotic drainage. The results also demonstrate that water content, degree of saturation and electric potential distributions can be used to deduce the electro-osmotic drainage process. Water content of soil decreases first near electrodes, while keeps nearly constant in the center of the model. The area with constant water content is larger than half of the sample surface. Moving anodes towards cathodes by about one third of the electrode spacing is effective to improve the treatment effect after electro-osmosis stops due to the large resistance. Moreover, it is observed that during electro-osmosis, the corrosion rate of anodes becomes smaller, while the variation in pH values of soil near anodes becomes larger. | Ying Li Xiaonan Gong Mengmeng Lu Yanli Tao | 2012 | Journal of Rock Mechanics and Geotechnical Engineering2012，4，3： | 2 |
| 17 | Activation of the bitter taste sensor TRPM5 prevents high saltinduced cardiovascular dysfunction显示文摘High salt intake is a known risk factor of cardiovascular diseases. Our recent study demonstrated that long-term high salt intake impairs transient receptor potential channel M5(TRPM5)-mediated aversion to high salt concentrations, consequently promoting high salt intake and hypertension;however, it remains unknown whether TRPM5 activation ameliorates cardiovascular dysfunction. Herein we found that bitter melon extract(BME) and cucurbitacin E(CuE), a major compound in BME, lowered high salt-induced hypertension. Long-term BME intake significantly enhanced the aversion to high salt concentrations by upregulating TRPM5 expression and function, eventually decreasing excessive salt consumption in mice. Moreover, dietary BME ameliorated high salt-induced cardiovascular dysfunction and angiotensin II-induced hypertension in vivo. The mechanistic evidence demonstrated that dietary BME inhibited high salt-induced RhoA/Rho kinase pathway overactivation, leading to reduced phosphorylation levels of myosin light chain kinase and myosin phosphatase targeting subunit 1. Furthermore, CuE inhibited vasoconstriction by attenuating L-type Ca^(2+) channel-induced Ca^(2+) influx in vascular smooth muscle cells. To summarize, our findings indicate that dietary BME has a beneficial role in antagonizing excessive salt consumption and thus appears promising for the prevention of high salt-induced cardiovascular dysfunction. | Hao Wu Yuanting Cui Chengkang He Peng Gao Qiang Li Hexuan Zhang Yanli Jiang Yingru Hu Xiao Wei Zongshi Lu Tianyi Ma Daoyan Liu Zhiming Zhu | 2020 | Science China(Life Sciences)2020，63，11： | 2 |
| 18 | Determination of optimal dietary selenium levels by full expression of selenoproteins in various tissues of broilers from 1 to 21 d of age显示文摘The current NRC dietary selenium(Se)requirement(0.15 mg/kg)of broilers is primarily based on growth performance data reported in 1986.Our study aimed to determine optimal dietary Se levels of broilers fed a practical corn-soybean meal diet for the full expression of selenoproteins in various tissues.A total of 384 one-d-old male broilers(n=8 replicates/diet)were fed a basal corn-soybean meal diet or the basal diet supplemented with 0.1,0.2,0.3,0.4 or 0.5 mg Se/kg in the form of Na_(2)SeO_(3) for 21 d.Regression analysis was conducted to evaluate the optimal dietary Se levels using broken-line,quadratic or asymptotic models.The activity of glutathione peroxidase(GPX)in the plasma,liver,kidney and pancreas,iodothyronine deiodinase(DIO)in the plasma,liver and pancreas,and thioredoxin reductase(Txnrd)in the liver and pancreas,the mRNA levels of Gpx1,Gpx4,Dio1,selenoprotein(Seleno)h,Selenop and Selenou in the liver,Gpx4,Dio1,Txnrd1,Txnrd2,Selenoh,Selenop and Selenou in the kidney,and Gpx1,Gpx4,Selenoh and Selenou in the pancreas,and the protein levels of GPX4 in the liver and kidney of broilers were influenced(P<0.05)by added Se levels,and increased quadratically(P<0.05)with the increase of added Se levels.The estimates of optimal dietary Se levels were 0.07 to 0.36 mg/kg based on the fitted broken-line,quadratic or asymptotic models(P<0.001)of the aforementioned selenoprotein expression in the plasma,liver and kidney,and 0.09 to 0.46 mg/kg based on the fitted broken-line models(P<0.001)of the aforementioned selenoprotein expression in the pancreas.The results indicate that the optimal dietary Se levels would be 0.36 mg/kg to support the full expression of selenoproteins in the plasma,liver and kidney,and 0.46 mg/kg to support the full expression of selenoproteins in the pancreas of broilers fed a practical corn-soybean meal diet from 1 to 21 d of age. | Xiudong Liao Guoqing Liu Guangming Sun Xiaoming Sun Tao Liu Lin Lu Liyang Zhang Minhong Zhang Yanli Guo Xugang Luo | 2021 | Animal Nutrition2021，，4： | 2 |
| 19 | Green tea consumption, inflammation and the risk of primary hepatocellular carcinoma in a Chinese population显示文摘 | Yanli Li Shen-Chih Chang Binh Y. Goldstein William L. Scheider Lin Cai Nai-Chieh Y. You Heather P. Tarleton Baoguo Ding Jinkou Zhao Ming Wu Qingwu Jiang Shunzhang Yu Jianyu Rao Qing-Yi Lu Zuo-Feng Zhang Lina Mu | 2011 | Cancer Epidemiology2011，，4： | 2 |
| 20 | Expression of Pseudorabies Virus gE Core Epitopes in Escherichia coli Strain BL21 and Utilization of Indirect PRV gE-ELISA显示文摘Pseudorabies virus glycoprotein E( PRV gE) has been recognized as a suitable diagnostic antigen for pseudorabies. In order to produce gE antigen in large quantities and at low cost,a gene fragment encoding PRV gE core epitopes was expressed in E. coli BL21 expression system. SDS-PAGE and Western Blotting revealed that the expression product in culture supernatant of E. coli BL21 was a recombinant protein,approximately 51. 8 Kd. At 5 h post-induction,protein concentration assay showed that the expression product amounted to 1. 65 mg /ml,accounting for 24. 17% of total proteins in the culture supernatant. An indirect PRV gE-ELISA was established by using the recombinant expression product as a coating antigen. Cross-reactivity assay showed that this antigen was PRV specific. In addition,the assay was consistently reproducible. Comparison of detection results of 240 serum samples between PRV gE-ELISA and a commercially available PRV diagnostic kit showed that there was no significant difference between these two methods( P > 0. 05). | Guangjun GUO Sufang LU Guanggang QU Feng LI Lin DONG Yanli BI Jinliang WANG Feng WEI Na TANG Chunling ZHANG Zhuang DING Zhiqiang SHEN | 2014 | Agricultural Biotechnology2014，3，4： | 2 |
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