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| 1 | Identification of tumor associated single-chain Fv by panning and screening antibody phage library using tumor cells显示文摘AIM:To study the feasibility of panning and screeningphage-displaying recombinant single-chain variablefragment (ScFv) of anti-tumor monoclonal antibodiesfor fixed whole cells as the carriers of mAb-bindingantigens.ME,ODS: The recombinant phage displaying librariesfor anti-colorectal tumor mAb MC3Ab, MC5Ab andanti-gastric tumor mAb MGD1 was constructed.Panning and screening were carried outby means ofmodified fixation of colorectal and gastric tumor cellsexpressed the mAb-binding antigens. Concordance ofbinding specificity to tumor cells between phageclones and parent antibodies was analyzed. Thephage of positive clones was identified withcompetitive ELISA, and infected by E.coli HB2151 toexpress soluble ScFv.RESULTS:The ratio of positive clones to MC3-ScF-MC5-ScFv and MGD1-ScFv were 60 %, 24 % and 30 %. MC3-ScFv had Mr 32 000 confirmed by Western blot. Thespecificity to antigen had no difference between 4positive recombinant phage antibodies and MC3Ab.CONCLUSION:The modified process of fixing wholetumor cells is efficient, convenient and feasible to panand screen the phage-displaying ScFv of anti-tumormonoclonal antibodies. | Nie, YZ He, FT Li, ZK Wu, KC Cao, YX Chen, BJ Fan, DM | 2002 | World Journal of Gastroenterology2002，8，4： | 12 |
| 2 | A New Isoflavone Glucoside from Astragalus membranaceus显示文摘A new isoflavone glucoside, 3’-methoxy-5’-hydroxy-isoflavone-7-O-β-D-glucoside, was isolated from the roots of Astragalus membranaceus and its structure waselucidated by spectroscopic data. | Cao, ZZ Cao, Y Yi, YJ Wu, YP Len, ZK Li, D Owen, NL | 1998 | Chinese Chemical Letters1998，9，6： | 4 |
| 3 | Comparison of percutaneous verte- broplasty with and without interventional tumour removal for malignant vertebral compression fractures with symptoms of neu- rological eompression显示文摘 | Li Y Gu YF Sun ZK | 2013 | Eur Radiol2013，23，10： | 1 |
| 4 | Highly water-dispersible biocompatible magnetite particles with low cytotoxicity stabilized by citrate groups显示文摘 | Liu J Sun ZK Deng YH Zou Y Li CY Guo XH | 2009 | Angew Chem Int Ed2009，48，： | 1 |
| 5 | Dragon moxibustion for 32 cases of low back muscle fasciitis显示文摘 | Yan ZK Huang W Li SL | 2014 | Zhongguo Zhen Jiu2014，34，6： | 1 |
| 6 | Effect of Deanxit,Omeprazole and Mosapride in patients of intractable gastroesophageal reflux disease显示文摘 | Li CH Sha ZK Shun GS | | 0，，06： | 1 |
| 7 | Molecular cloning of a novel tissue-specific gene from human nasopharyngeal epithelium 显示文摘 | Li ZK Yao KT Cao Y | 1999 | Gene1999，237，1： | 1 |
| 8 | Prognosyic vallie of serlm von willebrand factor,but not sollble icam and vcam,for mortality and cardiovasclilar events is independent of residlial renal function inperitoneal dialysis patients显示文摘 | Dong J Li YJ Yang ZK | 2014 | Perit Dial In2014，34，7： | 1 |
| 9 | Mapping of QTL with epistatic effects and QTL×environment interactions by mixed model approaches显示文摘 | Wang DL Zhu J Li ZK | 1999 | Theoretical and Applied Genetics1999，99，： | 1 |
| 10 | Development of an industrial medium for economical 2,3-butanediol production through co-fermentation of glucose and xylose by Klebsiella oxytoca显示文摘 | Ji X J Huang H Du J Zhu JG Ren L J Li S Nie ZK | 2009 | Bioresour Technol2009，100，21： | 1 |
| 11 | Mesenchymal stem cells alter migratory property of T and dendritic cells to delay the development of murine lethal acute graft-versus-host disease显示文摘 | Li H Guo ZK Jiang XX | 2008 | Stem Cells2008，26，： | 1 |
| 12 | Functional and phenotypic alteration of intrasplenic lymphocytes affected by mesenchymal stem cells in a murine allo-splenocyte transfusion model显示文摘 | Li H Guo ZK Li XS | 2007 | Cell transplantation2007，16，： | 1 |
| 13 | Epidemic of Klebsiella pneu-moniae ST11clone coproducing KPC-2and 16SrRNA methy-lase RmtB in a Chinese University Hospital显示文摘 | Li JJ Sheng ZK Deng M | 2012 | BMC InfectDis2012，12，： | 1 |
| 14 | CD73 + adipose-derived mesenchy- mal stem cells possess higher potential to differentiate into cardio- myocytes in vitro显示文摘 | Li Q Qi LJ Gun ZK | 2013 | J Mol Histol2013，44，4： | 1 |
| 15 | Downregulation of CDH13 expression promotes invasiveness of bladder transitional cell carcinoma显示文摘 | Lin YL He ZK Li ZG | 2013 | Urol Int2013，90，2： | 1 |
| 16 | Functional and phenotypic alteration of intrasplenic lymphocytes affected by mesenchymal stem cells in a murine allosplenocyte transfusion model 显示文摘 | Li H Guo ZK Li XS | 2007 | Cell Transplant2007，16，1： | 1 |
| 17 | Research of heroin addicts between buprenorphine and methadone 显示文摘 | LI JH Wan WP Feng ZK Yang MB Yang GY | 1993 | Zhongguo Shenjing Jingshen Zazhi (Chin J Nerv Ment Dis)1993，19，： | 1 |
| 18 | In vitro characteristics and in vivo immunosuppressive activity of compact bone-derived murine mesenchymal progenitor cells 显示文摘 | Guo ZK Li H Li XS | 2006 | Stem CeUs2006，2，10： | 1 |
| 19 | To compared the effect of total hip and built soldier prosthetic femoral head replacement therapy older femoral neck fracture 显示文摘 | LI L NIE ZK NIU JB | 2011 | Chinese and Foreign Medical2011，，4： | 1 |
| 20 | AN EXACT ANALYSIS OF THERMAL BUCKLING OF PIEZOELECTRIC LAMINATED PLATES显示文摘In this paper,the governing differential equations of elastic stability problems in ther-mopiezoelectric media are deduced.The solutions of the thermal buckling problems for piezoelectriclaminated plates are presented in the context of the mathematical theory of elasticity.Owing to thecomplexity of the eigenvalue problem involved,the critical temperature values of thermal bucklingmust be solved numerically.The numerical results for piezoelectric/non-piezoelectric laminated platesare presented and the influence of piezoelectricity upon thermal buckling temperature is discussed. | Shang, FL Wang, ZK Li, ZH | 1997 | Acta Mechanica Solida Sinica1997，10，2： | 1 |
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