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| 1 | Identification of tumor associated single-chain Fv by panning and screening antibody phage library using tumor cells显示文摘AIM:To study the feasibility of panning and screeningphage-displaying recombinant single-chain variablefragment (ScFv) of anti-tumor monoclonal antibodiesfor fixed whole cells as the carriers of mAb-bindingantigens.ME,ODS: The recombinant phage displaying librariesfor anti-colorectal tumor mAb MC3Ab, MC5Ab andanti-gastric tumor mAb MGD1 was constructed.Panning and screening were carried outby means ofmodified fixation of colorectal and gastric tumor cellsexpressed the mAb-binding antigens. Concordance ofbinding specificity to tumor cells between phageclones and parent antibodies was analyzed. Thephage of positive clones was identified withcompetitive ELISA, and infected by E.coli HB2151 toexpress soluble ScFv.RESULTS:The ratio of positive clones to MC3-ScF-MC5-ScFv and MGD1-ScFv were 60 %, 24 % and 30 %. MC3-ScFv had Mr 32 000 confirmed by Western blot. Thespecificity to antigen had no difference between 4positive recombinant phage antibodies and MC3Ab.CONCLUSION:The modified process of fixing wholetumor cells is efficient, convenient and feasible to panand screen the phage-displaying ScFv of anti-tumormonoclonal antibodies. | Nie, YZ He, FT Li, ZK Wu, KC Cao, YX Chen, BJ Fan, DM | 2002 | World Journal of Gastroenterology2002，8，4： | 12 |
| 2 | Treatment and complications of thalassemia major in Guangxi,Southern China显示文摘 | Yin XL Wu ZK He YY | 2011 | Pediatr Blood Cancer2011，57，： | 1 |
| 3 | Treatment and compli- cations of thalassemia major in Guangxi, Southern China 显示文摘 | Yin XL Wu ZK He YY | 2011 | Pediatr Blood Cancer2011，57，7： | 1 |
| 4 | Downregulation of protocadherin-10 expression correlates with malignant behaviour and poor prognosis in human bladder Cancer显示文摘 | Ma JG He ZK Ma JH | | 0，，01： | 1 |
| 5 | Magnetic microparticle-based multiplexed DNA detection with biobarcoded quantum dot probes 显示文摘 | Xiang DS Zeng GP He ZK | 2011 | Biosens Bioelectron2011，26，11： | 1 |
| 6 | Downregulation of CDH13 expression promotes invasiveness of bladder transitional cell carcinoma显示文摘 | Lin YL He ZK Li ZG | 2013 | Urol Int2013，90，2： | 1 |
| 7 | Determination of clenbuterol by ca-pillary electrophoresis immunoassay with chemiluminescence detection显示文摘 | Ji XH He ZK Ai XP | | 0，，02： | 1 |
| 8 | Treatment and complications of thalassemia major in Guangxi, Southern China显示文摘 | Yin XL Wu ZK He YY | 2011 | Pediatr Blood Cancer2011，57，7： | 1 |
| 9 | Nano-composite of poly(L-lactide) and surface grafted hydroxyapatite:Mechanical properties and biocompatibility显示文摘 | Hong ZK Zhang PB He CL | | 0，，： | 1 |
| 10 | Treatment and complications of thalassemia major in Guangxi, Southern China显示文摘 | Yin XL Wu ZK He YY | 2011 | Pediatr Blood Cancer2011，57，7： | 1 |
| 11 | Treatment and compli- cations ofthalassemia major in Guangxi,Southern China 显示文摘 | Yin XL Wu ZK He YY | 2011 | Pediatr Blood Cancer2011，57，7： | 1 |
| 12 | Nano-composite of poly (L-lactide) and surface grafted hydroxyapatite:Mechanical properties and biocompatibility显示文摘 | Hong ZK Zhang PB He CL | 2005 | Biomaterials2005，26，32： | 1 |
| 13 | Efficient Rh(I)-catalyzed directarylation and alkenylation of arene C-H bonds via decarbon-ylation of benzoic and cinnamic anhydrides 显示文摘 | Jin W Yu ZK He W | 2009 | Org Lett2009，11，6： | 1 |
| 14 | Treatment and complications of thalassemia major in Guangxi, Southern China显示文摘 | Yin XL Wu ZK He YY | 2011 | Pediatr Blood Cancer2011，57，7： | 1 |
| 15 | Downregulation of CDH13 expression promotes invasiveness of bladder transitional cell carcinoma显示文摘 | Lin YL He ZK Li ZG | 2013 | Urol Int2013，90，2： | 1 |
| 16 | Downregulation of protocadherin-10 expression correlates with malignant behavior and poor prognosis in human bladder cancer显示文摘 | Ma JG He ZK Ma JH | | 0，，： | 1 |
| 17 | Bifidobacteria exhibit LuxS-dependent autoinducer 2 activity and biofilm formation显示文摘 | Sun ZK He X Brancaccio VF | 2014 | PLoS One2014，9，88： | 1 |
| 18 | Treatment and complica- tions of thalassemia major in Guangxi, Southern China 显示文摘 | Yin XL Wu ZK He YY | 2011 | Pediatr Blood Cancer2011，57，7： | 1 |
| 19 | Treatment and complications of thalassemia major in Guangxi,Southern China显示文摘 | Yin XL Wu ZK He YY | 2011 | Pediatr Blood Cancer2011，57，7： | 1 |
| 20 | Determination of clenbuterol by capillary electrophoresis immunoassay with chemiluminescence detection显示文摘 | Ji XH He ZK Yang HY | | 0，，： | 1 |
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