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| 1 | Single-cell RNA-seq uncovers dynamic processes and critical regulators in mouse spermatogenesis显示文摘 | Yao Chen Yuxuan Zheng Yun Gao Zhen Lin Suming Yang Tongtong Wang Qiu Wang Nannan Xie Rong Hua Mingxi Liu Jiahao Sha Michael D. Griswold Jinsong Li Fuchou Tang Ming-Han Tong | 2018 | Cell Research2018，28，9： | 26 |
| 2 | Urimem,a membrane that can store urinary proteins simply and economically,makes the large-scale storage of clinical samples possible显示文摘By nature,biomarker is the measurable change associated with a physiological or pathophysiological process.Unlike blood which has mechanisms to minimize changes and to keep the internal environment homeostatic,urine is more likely to reflect changes of the body and is a better biomarker source.Because of its potential in biomarker discovery,urinary proteins should be preserved comprehensively as the duration of the patients’corresponding medical records.Here,we propose a method to adsorb urinary proteins onto a membrane we named Urimem.This simple and inexpensive method requires minimal sample handling,uses no organic solvents,and is environmentally friendly.Urine samples were filtered through the membrane,and urinary proteins were adsorbed onto the membrane.The proteins on the membrane were dried and stored in a vacuum bag,which keeps the protein pattern faithfully preserved.The membrane may even permit storage at room temperature for weeks.Using this simple and inexpensive method,it is possible to begin preserving urine samples from all consenting people.Thus,medical research especially biomarker research can be conducted more economically.Even more objective large-scale prospective studies will be possible.This method has the potential to change the landscape of medical research and medical practice. | JIA LuLu LIU XueJiao LIU Liu LI MingXi GAO YouHe | 2014 | Science China(Life Sciences)2014，57，3： | 19 |
| 3 | Perfecting and extending the near-infrared imaging window显示文摘In vivo fluorescence imaging in the second near-infrared window(NIR-II)has been considered as a promising technique for visualizing mammals.However,the definition of the NIR-II region and the mechanism accounting for the excellent performance still need to be perfected.Herein,we simulate the photon propagation in the NIR region(to 2340 nm),confirm the positive contribution of moderate light absorption by water in intravital imaging and perfect the NIR-II window as 900–1880 nm,where 1400–1500 and 1700–1880 nm are defined as NIR-IIx and NIR-IIc regions,respectively.Moreover,2080–2340 nm is newly proposed as the third near-infrared(NIR-III)window,which is believed to provide the best imaging quality.The wide-field fluorescence microscopy in the brain is performed around the NIRIIx region,with excellent optical sectioning strength and the largest imaging depth of intravital NIR-II fluorescence microscopy to date.We also propose 1400 nm long-pass detection in off-peak NIR-II imaging whose performance exceeds that of NIR-IIb imaging,using bright fluorophores with short emission wavelength. | Zhe Feng Tao Tang Tianxiang Wu Xiaoming Yu Yuhuang Zhang Meng Wang Junyan Zheng Yanyun Ying Siyi Chen Jing Zhou Xiaoxiao Fan Dan Zhang Shengliang Li Mingxi Zhang Jun Qian | 2021 | Light(Science & Applications)2021，10，10： | 4 |
| 4 | Specific Recognition of Breast Cancer Cells In Vitro Using Near Infrared-Emitting Long-Persistence Luminescent Zn_3Ga_2Ge_2O_(10):Cr^(3+)Nanoprobes显示文摘In this paper, near-infrared emitting long-persistence luminescent Zn3Ga2Ge2O10:Cr3?(ZGG) nanoparticles with diameters of 30–100 nm and bright luminescence were prepared by a sol–gel synthesis method. After the surface amination, the nanoparticles were further bioconjugated with breast cancer-specific monoclonal antibody(anti-Ep CAM) to form ZGG-Ep CAM nanoprobes which can specifically target breast cancer cell lines(MCF7) in vitro. The results of in vitro images show that the luminescence signals from the cells treated with ZGG-Ep CAM nanoprobes are stronger than those from cells treated with ZGG-unconjugated antibody, indicating that the prepared ZGG-Ep CAM nanoprobes possessed excellent specific recognition capability. Furthermore, due to their long afterglow properties, the imaging could persist more than 1 h. Therefore, these nanoprobes could not only provide a high specificity detection method for cancer cells but also realize the long-time monitoring. Developed near-infrared emitting long-persistence luminescent nanoprobes will be expected to find new perspectives for cell therapy research and diagnosis applications. | Jinlei Li Junpeng Shi Jiangshan Shen Huizi Man Mingxi Wang Hongwu Zhang | 2015 | Nano-Micro Letters2015，7，2： | 4 |
| 5 | Theoretical and experimental research on nonlinear spring models of a bonding interface显示文摘在二固体之间的一个结合的接口上的非线性的边界条件一纵或砍水平嘘,在正常发生下面的波浪被探索。由使用秒顺序不安方法,非线性的春天模型严厉地在小厚度的限制被开发到波长比率。数字结果同意很好,准确答案由连续边界条件获得了。为纵的波浪的非线性的春天模型被测量钢或铝底层的楔形的结合结构的非线性验证。试验性的结果表明上述模型是相当精确的,当到粘合剂的 adherend 的阻抗比率增加,模型变得更精确。 | AN Zhiwu WANG Xiaomin MAO Jie LI Mingxuan DENG Mingxi | 2012 | Chinese Journal of Acoustics2012，31，2： | 4 |
| 6 | Securing Multicast Route Discovery for Mobile Ad Hoc Networks显示文摘This paper presents SMRAN, a novel securing multicast route discovery scheme for mobile ad hoc networks. The scheme relies entirely on hash chains based one-time signature mechanism, HORSEI, with very efficient signing and verifying, and we do this by improving the HORSE protocol through the introduction of interme- diate hash joints. The main purpose of SMRAN is to provide source authentication for multicast routing discovery messages in mobile ad hoc networks. SMRAN will construct multicast tree with authentica- tion constrains in ad hoc networks. The performance measure of SMRAN is evaluated using simulator NS2. The results represent that SMRAN produces less end-to-end packet latency than public key based secure routing scheme, and it is a feasible approach to securing multicast routing for mobile ad hoc networks. | YANG Mingxi LI Layuan FANG Yiwei | 2007 | Wuhan University Journal of Natural Sciences2007，12，1： | 3 |
| 7 | Quantum dots assisted in vivo two-photon microscopy with NIR-Ⅱemission显示文摘With the advantages of high resolution and deep penetration depth,two-photon excited NIR-Ⅱ(900–1880 nm)fluorescence(2PF)microscopic bioimaging is promising.However,due to the lack of imaging systems and suitable probes,few such works,to our best knowledge,were demonstrated utilizing NIR-II excitation and NIR-Ⅱfluorescence simultaneously.Herein,we used aqueously dispersible Pb S/Cd S quantum dots with bright NIR-II fluorescence as the contrast agents.Under the excitation of a 1550 nm femtosecond(fs)laser,they emitted bright 2 PF in the NIR-Ⅱregion.Moreover,a 2PF lifetime imaging microscopic(2PFLIM)system was implemented,and in vivo 2PFLIM images of mouse brain blood vessels were obtained for the first time to our best knowledge.To improve imaging speed,an in vivo two-photon fluorescence microscopy(2 PFM)system based on an In Ga As camera was implemented,and in vivo 2PFM images of QDs-stained mouse brain blood vessels were obtained. | HUWEI NI YALUN WANG TAO TANG WENBIN YU DONGYU LI MUBIN HE RUNZE CHEN MINGXI ZHANG JUN QIAN | 2022 | Photonics Research2022，10，1： | 3 |
| 8 | Sol-gel preparation and phosphorescence property of Mn^(2+)-doped zinc boro-silicate glass thin films显示文摘Mn2+-doped zinc borosilicate (ZBSM) glass thin films were first synthesized by sol-gel method. In the experiment, a thin gel film was depos-ited onto quartz glass substrates by dip-coating method and then heat-treated to form a Mn2+-doped zinc borosilicate glass thin film. Long lasting phosphorescence (LLP) and photo-stimulated long lasting phosphorescence (PSLLP) were found in the film sample. According to fluorescence spectra, LLP emission spectra, and PSLLP emission spectra, both LLP and PSLLP emissions are attributed to the energy level transition of 4Eg→4A1g from Mn2+. Both the phosphorescence intensity decay curves contain a fast decay component and another slow decay one. The thermoluminescence (TL) spectra show that the sample has two kinds of traps at least and their energy level values are about 0.8 eV and 1.02 eV, which could be estimated by the Randall and Willcins formula. The infrared absorption spectra (IR) consist of characteristic vi-bration bands of Si-O-Si, Si-O-Zn, B-O in [BO3], B-O group, and Zn-O in [ZnO4]. Moreover, image storage and logical operation of the ZBSM film were carried out successfully through an experiment analogues of optical storage. | LI Fengfeng ZHANG Mingxi HOU Guiqin SHEN Yi LIU Zhigang LI Hongsheng | 2011 | Rare Metals2011，30，3： | 2 |
| 9 | Chiral polymer-based biointerface materials显示文摘Chirality is a unique phenomenon in nature. Chiral interactions play an important role in biological and physiological processes, which provides much inspiration for scientists to develop chiral materials. As a breakthrough from traditional materials, biointerface materials based on chiral polymers have attracted increasing interest over the past few years. Such materials elegantly combine the advantages of chiral surfaces and traditional polymers, and provide a novel solution not only for the investigation of chiral interaction mechanisms but also for the design of biomaterials with diverse applications, such as in tissue engineering and biocompatible materials, bioregulation, chiral separation and chiral sensors. Herein, we summarize recent advances in the study of chiral effects and applications of chiral polymer-based biointerface materials, and also present some challenges and perspectives. | LI MinMin QING GuangYan ZHANG MingXi SUN TaoLei | 2014 | Science China Chemistry2014，57，4： | 2 |
| 10 | Genetically modified CD7-targeting allogeneic CAR-T cell therapy with enhanced efficacy for relapsed/refractory CD7-positive hematological malignancies:a phase I clinical study显示文摘Chimeric antigen receptor(CAR)-T cell therapy against T cell malignancies faces major challenges including fratricide between CAR-T cells and product contamination from the blasts.Allogeneic CAR-T cells,generated from healthy donor T cells,can provide ready-to-use,blast-free therapeutic products,but their application could be complicated by graft-versus-host disease(GvHD)and host rejection.Here we developed healthy donor-derived,CD7-targeting CAR-T cells(RD13-01)with genetic modifications to resist fratricide,GvHD and allogeneic rejection,as well as to potentiate antitumor function.A phase I clinical trial(NCT04538599)was conducted with twelve patients recruited(eleven with T cell leukemia/lymphoma,and one with CD7-expressing acute myeloid leukemia).All patients achieved pre-set end points and eleven proceeded to efficacy evaluation.No dose-limiting toxicity,GvHD,immune effector cell-associated neurotoxicity or severe cytokine release syndrome(grade≥3)were observed.28 days post infusion,81.8%of patients(9/11)showed objective responses and the complete response rate was 63.6%(7/11,including the patient with AML).3 of the responding patients were bridged to allogeneic hematopoietic stem cell transplantation.With a median follow-up of 10.5 months,4 patients remained in complete remission.Cytomegalovirus(CMV)and/or Epstein-Barr virus(EBV)reactivation was observed in several patients,and one died from EBV-associated diffuse large B-cell lymphoma(DLBCL).Expansion of CD7-negative normal T cells was detected post infusion.In summary,we present the first report of a Phase I clinical trial using healthy donor-derived CD7-targeting allogeneic CAR-T cells to treat CD7^(+)hematological malignancies.Our results demonstrated the encouraging safety and efficacy profiles of the RD13-01 allogeneic CAR-T cells for CD7^(+)tumors. | Yongxian Hu Yali Zhou Mingming Zhang Houli Zhao Guoqing Wei Wengang Ge Qu Cui Qitian Mu Gong Chen Lu Han Tingting Guo Jiazhen Cui Xiaoyan Jiang Xiujun Zheng Shuhui Yu Xiaolong Li Xingwang Zhang Mingxi Chen Xiuju Li Ming Gao Kang Wang Cheng Zu Hao Zhang Xiaohong He Yanbin Wang Dongrui Wang Jiangtao Ren He Huang | 2022 | Cell Research2022，32，11： | 2 |
| 11 | Specific Determination of Unbound Oxacillin in Protein Solution with Cefoperazone by High-performance Frontal Analysis with Chemiluminescence Detection显示文摘 | Li Famei Qiao Mingxi Guo Xingjie | 2003 | Biomed Chromatogr2003，17，1： | 1 |
| 12 | Laser cladding Co?based alloy/SiCp composite coatings on IF steel显示文摘 | Li Mingxi He Yizhu Sun Guoxiong | 2004 | Materials and Design2004，25，： | 1 |
| 13 | Capsid destabilization and epitope alterations of human papillomavirus 18 in the presence of thimerosal显示文摘Thimerosal has been widely used as a preservative in drug and vaccine products for decades.Due to the strong propensity to modify thiols in proteins,conformational changes could occur due to covalent bond formation between ethylmercury(a degradant of thimerosal)and thiols.Such a conformational change could lead to partial or even complete loss of desirable protein function.This study aims to investigate the effects of thimerosal on the capsid stability and antigenicity of recombinant human papillomavirus(HPV)18 virus-like particles(VLPs).Dramatic destabilization of the recombinant viral capsid upon thimerosal treatment was observed.Such a negative effect on the thermal stability of VLPs preserved with thimerosal was shown to be dependent on the thimerosal concentration.Two highly neutralizing antibodies,13H12 and 3C3,were found to be the most sensitive to thimerosal treatment.The kinetics of antigenicity loss,when monitored with 13H12 or 3C3 as probes,yielded two distinctly different sets of kinetic parameters,while the data from both monoclonal antibodies(mAbs)followed a biphasic exponential decay model.The potential effect of thimerosal on protein function,particularly for thiolcontaining proteinaceous active components,needs to be comprehensively characterized during formulation development when a preservative is necessary. | Xiaofen Huang Yike Li Meifeng Nie Mingxi Yue Yufang Li Zhijie Lin Huirong Pan Mujin Fang Ting Wu Shaowei Li Jun Zhang Ningshao Xia Qinjian Zhao | 2021 | Journal of Pharmaceutical Analysis2021，11，5： | 1 |
| 14 | Influence of the C2H2flow rate on gradient Ti CN films deposited by multi-arc ion plating显示文摘 | Li Jinlong Zhang Shihong Li Mingxi | 2013 | Applied Surface Science2013，283，： | 1 |
| 15 | Effect of the addition of Sm2 03 on the microstructure of laser cladding alloy coating layers显示文摘 | Zhang Shihong Li Mingxi Jae Hong Yoon | 2008 | Metals and Materials International2008，14，3： | 1 |
| 16 | Characterization on the coatings of Ni-base alloy with nano-and micron-size Sm2O3addition prepared by laser deposition显示文摘 | ZHANGShihong LI mingxi YOON Jae Hong CHU Tong Yul | 2008 | Materials Chemistry and Physics2008，112，： | 1 |
| 17 | Microstructure and wear resistance of laser clad Cobalt-based alloy mutilayer coatings显示文摘 | Li Mingxi He Yizhu Sun Guoxiong | 2004 | Applied Surface Science2004，230，2： | 1 |
| 18 | Micrnstructure and wear resistance of laser clad cobah - based alloy mahi - layer coatings 显示文摘 | Li Mingxi He Yizhu Sun Guoxiong | 2004 | Application Surface Science2004，230，： | 1 |
| 19 | Synthesis and character-ization of Cu3 N - WC nano composite films prepared by direct currentmagnetron sputtering 显示文摘 | Shihong Zhang Yinsheng He Mingxi Li | 2010 | Thin Solid Films2010，518，： | 1 |
| 20 | A superhard CrAlSiN superlattice coating deposited by multi-arc ion plating: I. Microstructure and mechanical properties显示文摘 | Shihong Zhang Lei Wang Qimin Wang Mingxi Li | 2013 | Surface & Coatings Technology2013，，： | 1 |
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