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| 1 | Gene expression profiling under different photoperiod/temperature conditions in a photoperiod-/thermo-sensitive genic male sterile line of rice(Oryza sativa L.)显示文摘Peiai 64S is a photoperiod- and thermo- sensitive genic male sterile (PTSGMS) line of rice, which is male sterile at long day/high temperature and partial fertile at short day/low temperature. A cDNA array representing 3328 unique rice genes was used to profile the gene expression patterns in the young panicles of Peiai 64S under these two condi- tions. The statistical data showed that up to 14.60% of genes exhibited up-or down-regulated expressions in the plants at long day/high temperature compared with plants at short day/low temperature. Only four genes were up-regulated while 482 genes down-regulated. Real-time PCR with all the up-regulated and 9 randomly selected down-regulated genes confirmed the differential expressions detected by the array, indicating that the constructed cDNA array is reliable. These differently expressed genes participated in almost all cell biological responses. Analysis of up- and down-regulated genes revealed distinctive changes between the mRNA abundances of MMK1 and MMK2, both of which are analogs ofMAPK, and significant down-regulation of several transcription factors. It was suggested that changes that occurred in the MAPK signal transduction path- way might disturb the transcription control necessary for morphogenesis of pollens and corresponding phys- iological functions. | GUO Xiaoqin DONG Haitao ZHENG Kangle LUO Hongmei TAN Xuelin FANG Yongqi WANG Yanqin DENG Ye DAI Chengen LOU Yichun SHAO Jing SHI Wenqin ZHAO Dong LI Debao | 2006 | Chinese Science Bulletin2006,51,2: | 2 |
| 2 | An Improved ZigBee rou- ting strategy for monitoring system显示文摘 | Xiaohui Li Kangling Fang Jinguang Gu | 2008 | Intelligent Networks and In- telligent Systems2008,19,6: | 1 |
| 3 | A neural-fuzzy control in resistance furnace显示文摘 | FANG Kangling SHEN Zhongjie | 1997 | IEEE Intelligent Processing Sys- tems1997,35,16: | 1 |
| 4 | WSNHA- GAHR : A Greedy and A* Heuristic Routing Algorithm for Wireless Sensor Networks in Home Automation显示文摘 | Li Xiaohui Hong Sung Ho Fang Kangling | 2011 | IET Communications2011,5,13: | 1 |
| 5 | Redesigning transcription factor Cre1 for alleviating carbon catabolite repression in Trichoderma reesei显示文摘Carbon catabolite repression(CCR),which is mainly mediated by Cre1 and triggered by glucose,leads to a decrease in cellulase production in Trichoderma reesei.Many studies have focused on modifying Cre1 for alleviating CCR.Based on the homologous alignment of CreA from wild-type Penicillium oxalicum 114–2(Po-0)and cellulase hyperproducer JUA10-1(Po-1),we constructed a C-terminus substitution strain—Po-2—with decreased transcriptional levels of cellulase and enhanced CCR.Results revealed that the C-terminal domain of CreAPo−1 plays an important role in alleviating CCR.Furthermore,we replaced the C-terminus of Cre1 with that of CreAPo−1 in T.reesei(Tr-0)and generated Tr-1.As a control,the C-terminus of Cre1 was truncated and Tr-2 was generated.The transcriptional profiles of these transformants revealed that the C-terminal chimera greatly improves cellulase transcription in the presence of glucose and thus upregulates cellulase in the presence of glucose and weakens CCR,consistent with truncating the C-terminus of Cre1 in Tr-0.Therefore,we propose constructing a C-terminal chimera as a new strategy to improve cellulase production and alleviate CCR in the presence of glucose. | Lijuan Han Kuimei Liu Wei Ma Yi Jiang Shaoli Hou Yinshuang Tan Quanquan Yuan Kangle Niu Xu Fang | 2020 | Synthetic and Systems Biotechnology2020,5,3: | 0 |
| 6 | 毛竹的耐铝性研究显示文摘毛竹广泛分布于中国的南方的酸性土壤区域,那些区域是铝毒性的高风险地区。为了对毛竹对铝的抗性的进行评估,本文对相同生长进程下的毛竹和两个水稻栽培品种的种子萌发和根的延长生长进行了对比,并仔细观察了他们在各级不同铝的受力下的物理和生理的损害程度。结果表明:铝浓度增加至500μM时,毛竹种子萌发受到抑制,半致死浓度为2000μM。相比之下,水稻种子萌发并没有受到铝浓度的抑制,甚至在铝浓度为2000μM时。铝积累主要在根尖细胞壁和原生质体治疗时间的延长进入原生质体和/或随着铝浓度的增加,这种现象导致细胞凋亡。竹子的根表皮在2000μM铝浓度时存在显著的退化。总之,毛竹只对低浓度的铝具有抗性。 | Renvi Gui Huanan Leng Shunyao Zhuang Kangle Zheng Wei Fang | 2012 | 世界竹藤通讯2012,,5: | 0 |
| 7 | Multiple splicing types of OsRIX4,an RAD21 homolog in rice (Oryza sativa L.)显示文摘The present paper describes multiple splicing types of OsRIX4,an RAD21 homolog in rice. A type of alternative splicing (AS),distinctive from all five previously known splicing types,was identified in which interior sequences of a constitutive exon could be spliced. Translation of the transcript produced with this AS type was demonstrated at the protein level. Expression of multiple transcripts was organ specific. The expression abundance of transcripts,OsRIX4-4 and OsRIX4-5,was positively correlated with fertility in rice. The splicing type identified in the present study provided the means to further un-derstand and define different mRNA splicing types in plants and suggested that post-transcription processing of mRNA and its regulation mechanism are complex. | DONG HaiTaoi GUO XiaoQin PEI YanXi DAI ChengEn FANG YongQi TU QiChao ZHUANG JieYun ZHAO Dong ZHENG KangLe LI DeBao | 2007 | Chinese Science Bulletin2007,52,11: | 0 |
| 8 | Feasibility of feeding cadmium accumulator maize(Zea mays L.)to beef cattle:Discovering a strategy for eliminating phytoremediation residues显示文摘Eco-friendly and efficient strategies for eliminating cadmium(Cd)phytoremediation plant residues are needed.The present study investigated the feasibility of feeding Cd accumulator maize to beef cattle.In total,20 cattle at 6 months of age were selected and randomly allocated into two groups fed with 85.82%(fresh basis)Cd accumulator maize(CAM)or normal maize(control[Con])silage diets for 107 d.Feeding CAM did not affect the body weight(P=0.24),while it decreased feed intake and increased feed efficiency of beef cattle(P<0.01).Feeding CAM increased serum concentrations of immunoglobulin A and G,complement 3 and 4,blood urea nitrogen,and low-density lipoprotein cholesterol,decreased serum concentrations of interleukin-6 and lipopolysaccharide(P<0.05),and caused wider lumens in the renal tubules.The Cd residue in meat was 7 mg/kg beyond the restriction for human food.In the muscle,the unsaturated fatty acids(t11C18:1 and C20:4),Lys,Arg,Pro,and Cys were decreased,while the saturated fatty acids(C10:0,C12:0,and C17:0)and Leu were increased(P<0.05).Therefore,at the current feeding level,phytoremediation maize increased the feed efficiency of beef cattle,but did present risks to cattle health and production safety,and decreased the meat nutrition and flavor.Further research must be performed to determine whether a lower proper dose of phytoremediation maize and an appropriate feeding period may be possible to ensure no risk to cattle health and the supply of safe meat for humans. | Zebang Xu Bin Yang Kangle Yi Tianrong Chen Xinxin Xu Ao Sun Haobang Li Jianbo Li Fang He Cheng Huan Yang Luo Jiakun Wang | 2023 | Animal Nutrition2023,,4: | 0 |