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| 1 | Effect of Long-Term Rice Straw Return on Soil Glomalin, Carbon and Nitrogen显示文摘A long-term experiment was conducted to investigate how long-term fertilization and rice straw incorporation into soil affect soil glomalin, C and N. The combined application of chemical fertilizer and straw resulted in a significant increase in both soil easily extractable glomalin (EEG) and total glomalin (TG) concentrations, as compared with application of only chemical fertilizer or no fertilizer application. The EEG and TG concentrations of the NPKS (nitrogen, phosphorus, and potassium fertilizer application + rice straw return) plot were 4.68% and 5.67% higher than those of the CK (unfertilized control) plot, and 9.87% and 6.23% higher than those of the NPK (nitrogen, phosphorus, and potassium fertilizer applied annually) plot, respectively. Application of only chemical fertilizer did not cause a statistically significant change of soil glomalin compared with no fertilizer application. The changes of soil organic C (SOC) and total N (TN) contents demonstrated a similar trend to soil glomalin in these plots. The SOC and TN contents of NPKS plot were 15.01% and 9.18% higher than those of the CK plot, and 8.85% and 14.76% higher than those of the NPK plot, respectively. Rice straw return also enhanced the contents of microbial biomass C (MBC) and microbial biomass N (MBN) in the NPKS plot by 7.76% for MBC and 31.42% for MBN compared with the CK plot, and 12.66% for MBC and 15.07% for MBN compared with the NPK plots, respectively. Application of only chemical fertilizer, however, increased MBN concentration, but decreased MBC concentration in soil. | NIE Jun ZHOU Jian-Min WANG Huo-Yan CHEN Xiao-Qin DU Chang-Wen | 2007 | Pedosphere2007，17，3： | 48 |
| 2 | Effects of Long-Term Winter Planted Green Manure on Physical Properties of Reddish Paddy Soil Under a Double-Rice Cropping System显示文摘Soil physical properties are important indicators of the potential for agricultural production.Our objective was to evaluate the effects of long-term inputs of green manures on physical properties of a reddish paddy soil(Fe-Typic Hapli-Stagnic Anthrosols) under a double cropping system.The common cropping pattern before the study was early-late rice-fallow(winter).The field treatments included rice-rice-fallow(R-R-WF),rice-rice-rape(R-R-RP),rice-rice-Chinese milk vetch(RR-MV),and rice-rice-ryegrass(R-R-RG).The rape,Chinese milk vetch and ryegrass were all incorporated as green manures 15 d before early rice transplanting during the following year.The soil bulk density in all green manure treatments was significantly reduced compared with the winter fallow treatment.Soil porosity with green manure applications was significantly higher than that under the winter fallow.The green manure treatments had higher 0.25-5 mm water stable aggregates and aggregates stabilities in the plow layer(0-15 cm depth) compared with the fallow treatment.The mean weight diameter(MWD) and normalized mean weight diameter(NMWD) of aggregates in the green manure treatment were larger than that with the winter fallow.Soil given green manure retained both a higher water holding capacity in the plow layer soil,and a larger volume of moisture at all matric potentials(-10,-33 and-100 kPa).We conclude that the management of double-rice fields in southern central China should be encouraged to use green manures along with chemical fertilizers to increase SOC content,improve soil physical properties and soil fertility. | YANG Zeng-ping XU Ming-gang ZHENG Sheng-xian NIE Jun GAO Ju-sheng LIAO Yu-lin XIE Jian | 2012 | Journal of Integrative Agriculture2012，11，4： | 40 |
| 3 | Effects of Long-Term Winter Planted Green Manure on Distribution and Storage of Organic Carbon and Nitrogen in Water-Stable Aggregates of Reddish Paddy Soil Under a Double-Rice Cropping System显示文摘In agricultural systems, maintenance of soil organic matter has long been recognized as a strategy to reduce soil degradation. Manure amendments and green manures are management practices that can increase some nutrient contents and improve soil aggregation. We investigated the effects of 28 yr of winter planted green manure on soil aggregate-size distribution and aggregateassociated carbon(C) and nitrogen(N). The study was a randomized completed block design with three replicates. The treatments included rice-rice-fallow, rice-rice-rape, rice-rice-Chinese milk vetch and rice-rice-ryegrass. The experiment was established in 1982 on a silty light clayey paddy soil derived from Quaternary red clay(classified as Fe-Accumuli-Stagnic Anthrosols) with continuous early and late rice. In 2009, soil samples were collected(0-15 cm depth) from the field treatment plots and separated into water-stable aggregates of different sizes(i.e., >5, 2-5, 1-2, 0.5-1, 0.25-0.5 and <0.25 mm) by wet sieving. The long-term winter planted green manure significantly increased total C and N, and the formation of the 2-5-mm water-stable aggregate fraction. Compared with rice-rice-rape, rice-rice-Chinese milk vetch and rice-rice-ryegrass, the rice-rice-fallow significantly reduced 2-5-mm water-stable aggregates, with a significant redistribution of aggregates into micro-aggregates. Long-term winter planted green manure obviously improved C/N ratio and macro-aggregate-associated C and N. The highest contribution to soil fertility was from macro-aggregates of 2-5 mm in most cases. | YANG Zeng-ping ZHENG Sheng-xian NIE Jun LIAO Yu-lin XIE Jian | 2014 | Journal of Integrative Agriculture2014，13，8： | 32 |
| 4 | Photo-curing 3D printing technique and its challenges显示文摘In recent ten years,3D printing technology has been developed rapidly.As an advanced technology,3D printing has been used to fabricate complex and high-precision objects in many fields.3D printing has several technologies.Among these technologies,photo-curing 3D printing was the earliest and most mature technology.In 1988,the first 3D printing machine which was based on photo-curing and called Stereo lithography Appearance(SLA)technology was produced by 3D system Corp.After 30 years of development,many new technologies based on photocuring mechanism emerged.Based on the different principle of pattern formation and character of printing technology,numerous photocuring 3D printing techniques,such as SLA,DLP,LCD,CLIP,MJP,twophoton 3D printing,holographic 3D printing and so on,have been developed.Photo-curing 3D printing has many advantages,such as high precision,smooth surface of printing objects,rapid printing speed and so on.Here,we would introduce five industrial photocuring 3D printing technologies,which are SLA,DLP,LCD,CLIP and MJP.The characters of the materials and the progress of the application of the technique in the biomedical field is also overviewed.At last,the difficulties and challenges of photo-curing 3D printing are also discussed. | Haoyuan Quan Ting Zhang Hang Xu Shen Luo Jun Nie Xiaoqun Zhu | 2020 | Bioactive Materials2020，5，1： | 33 |
| 5 | Long-Term Effect of Fertilizer and Rice Straw on Mineral Composition and Potassium Adsorption in a Reddish Paddy Soil显示文摘Increasing K+ adsorption can be an effective alternative in building an available K pool in soils to optimize crop recovery and minimize losses into the environment. We hypothesized that long-term fertilization might change K+ adsorption because of changes in the chemical and mineralogical properties of a rice (Oryza sativa L.). The aims of this study were (i) to determine clay minerals in paddy soil clay size fractions using X-ray diffraction methods and a numerical diagram-decomposition method; (ii) to measure K+ adsorption isotherms before and after H 2 O 2 oxidation of organic matter, and (iii) to investigate whether K+ adsorption is correlated with changes in soil chemical and mineral properties. The 30-yr long-term fertilization treatments caused little change in soil organic C (SOC) but a large variation in soil mineral composition. The whole-clay fraction (<5 μm) corresponded more to the fertilization treatment than the fine-clay fraction (<1 μm) in terms of percentage of illite peak area. The total percentage of vermiculite-chlorite peak area was significantly negatively correlated with the total percentage of illite peak area in the <5 μm soil particles (R=-0.946, P<0.0006). Different fertilization treatments gave significantly different results in K+ adsorption. The SOC oxidation test showed positive effects of SOC on K+ adsorption at lower K+ concentration ( 120 mg L-1) and negative effects at higher K+ concentration (240 mg L-1). The K+ adsorption by soil clay minerals after SOC oxidization accounted for 60-158% of that by unoxidized soils, suggesting a more important role of soil minerals than SOC on K+ adsorption. The K+ adsorption potential was significantly correlated to the amount of poorly crystallized illite present (R=0.879, P=0.012). The availability of adsorbed K+ for plant growth needs further study. | LIAO Yu-lin ZHENG Sheng-xian NIE Jun XIE Jian LU Yan-hong QIN Xiao-bo | 2013 | Journal of Integrative Agriculture2013，12，4： | 30 |
| 6 | 5-Hydroxymethylcytosine signatures in circulating cell-free DNA as diagnostic biomarkers for human cancers显示文摘象 5-methylcytosine (5mC ) 和 5-hydroxymethylcytosine (5hmC ) 那样的 DNA 修正是知道在哺乳动物影响全球基因表示的 epigenetic 标记。在人的染色体,与基因表示的靠近的关联和高化学的稳定性给他们的流行,这些 DNA epigenetic 标记能为癌症用作理想的 biomarkers 诊断。利用一种高度敏感、选择的化学标记技术,我们这里报导在传播没有房间的 DNA ( cfDNA )并且在从最近与 colorectal 诊断的 260 个病人的一个队收集的配对的肿瘤和邻近的纸巾的 genomic DNA ( gDNA )的 5hmC 的染色体宽的介绍,胃,胰腺,从 90 个健康个人的肝或甲状腺癌症和正常纸巾。5hmC 主要在与开的染色质和容许的 histone 修正重合的 transcriptionally 活跃的区域被散布。柔韧的联系癌症的 5hmC 签名在 cfDNA 被识别为特定的癌症类型是特征的。传播 cfDNA 的 5hmC 底 biomarkers colorectal 和胃的癌症是高度预兆的并且比常规 biomarkers 优异并且比得上从织物活体检视的 5hmC biomarkers。因此,这新策略能从血样品的分析为癌症的诊断和预后导致有效、最低限度地侵略的方法的发展。 | Wenshuai Li Xu Zhang Xingyu Lu Lei You Yanqun Song Zhongguang Luo Jun Zhang Ji Nie Wanwei Zheng Diannan Xu Yaping Wang Yuanqiang Dong Shulin Yu Jun Hong Jianping Shi Hankun Hao Fen Luo Luchun Hua Peng Wang Xiaoping Qian Fang Yuan Lianhuan Wei Ming Cui Taiping Zhang Quan Liao Menghua Dai Ziwen Liu Ge Chen Katherine Meckel Sarbani Adhikari Guifang Jia Marc B Bissonnette Xinxiang Zhang Yupei Zhao Wei Zhang Chuan He Jie Liu | 2017 | Cell Research2017，27，10： | 30 |
| 7 | Fast track clinical pathway implications in esophagogastrectomy显示文摘AIM:To investigate the feasibility of fast track clinical pathway for esophageal tumor resections. METHODS:One hundred and fourteen patients with esophageal carcinoma who underwent esophagogastrectomy from January 2006 to October 2007 in our department were studied.Fast track clinical pathway included analgesia control,fluid infusion volume control,early ambulation and enteral nutrition. Nasogastric tube was removed 3 d after operation and chest tube was removed 4 d after operation as a routine,and full liquid diet 5 d after operation. RESULTS:Among 114 patients(84 men and 30 women),26 patients underwent fast track surgery,including 17 patients over 65 years old and 9 under 65(P=0.014);18 patients who had preoperative complications could not bear fast track surgery(P< 0.001).No significant differences in tolerance of fast track surgery were attributed to differences in gender,differentiated degree or stage of tumor,pathological type of tumor,or operative incision.The median length of hospital stay was 7 d(5-28 d),4%patients were readmitted to hospital within 30 d of discharge.Three patients died and postoperative mortality was 2.6%. All 3 patients had no determinacy to fast track surgery approach.CONCLUSION:The majority of patients with esophageal carcinoma can tolerate fast track surgery. Patients younger than 65 or who have no preoperative diseases have the best results.Median length of hospital stay has been reduced to 7 d. | Ke Jiang Lin Cheng Jian-Jun Wang Jin-Song Li Jun Nie | 2009 | World Journal of Gastroenterology2009，15，4： | 24 |
| 8 | Association between Vitamin D Insufficiency and the Risk for Gestational Diabetes Mellitus in Pregnant Chinese Women显示文摘Objective To investigate the association between vitamin D deficiency and risk of gestational diabetes mellitus(GDM) in pregnant Chinese women.Methods A nested case-control study was conducted.Clinical and biochemical data were analyzed for 200 subjects with GDM and 200 subjects with normal glucose tolerance(NGT).Results The median(interquartile range) serum 25-hydroxyvitamin D(25OHD) levels were 22.39(17.67,29.38) and 25.86(19.09,34.88) nmol/L in the GDM and NGT groups,respectively.Rates of 25OHD deficiency or insufficiency were significantly higher in the GDM group than in the NGT group.Subjects with 25OHD levels <25 nmol/L had a 1.8-fold higher risk of GDM compared with subjects with higher vitamin D levels.In the GDM group,serum 25OHD was independently associated with HbA1c and insulin resistance after adjusting for confounding factors.In the NGT group,serum 25OHD was independently associated with fasting plasma glucose and systolic blood pressure after adjusting for maternal age and other confounding factors.Conclusion 25OHD insufficiency is very common in Chinese women.Low 25OHD status may be associated with insulin resistance and act as a risk factor for GDM. | WANG Ou NIE Min HU Ying Ying ZHANG Kui LI Wei PING Fan LIU Jun Tao CHEN Li Meng XING Xiao Ping | 2012 | Biomedical and Environmental Sciences2012，25，4： | 21 |
| 9 | Intercontinental disjunctions between eastern Asia and western North America in vascular plants highlight the biogeographic importance of the Bering land bridge from late Cretaceous to Neogene显示文摘这评论在脉管的植物(flowering 植物,裸子植物,蕨纲植物和 lycophytes ) 的主要的系从迟了的白垩纪显示出在东方亚洲和西方的北美洲之间的一个靠近的 biogeographic 连接到迟了的晚第三纪。东方亚洲北方的美国 disjuncts,针叶树展出在东方亚洲和西方的北美洲之间的 disjuncts 的一个高比例。蕨纲植物的几个系也在二个区域显示出一个最近的分离模式。在 flowering 植物,模式通常在适度的元素在之间被显示出东北亚洲和西北的北美洲,以及寡妇 boreotropical 和晚第三纪 mesophytic 和具球果的植物群的元素。在在最近的种系发生的分析支持的植物的东方亚洲和西方的北美洲之间的洲际 biogeographic 分离的许多盒子从迟了的白垩纪越过 Beringian 区域强调 Bering 陆地桥或植物迁居的重要性到迟了的晚第三纪,在特别期间中新世。Beringian 区域允许自从上新世,在白令海峡的开始以后过滤和某些植物 taxa 的移植,作为许多同种的 taxa 或密切相关的种类发生在 Beringia 的双方上。 | Jun Wen Ze-Long Nie Stefanie M. Ickert-Bond | 2016 | Journal of Systematics and Evolution2016，54，5： | 20 |
| 10 | 胆汁酸与肠道微生物的对话机制及其在宿主代谢与健康中的作用(英文)显示文摘胆汁酸只在肝脏中合成,并通过直接作用或间接作用抑杀肠道微生物。而肠道微生物又可以促进初级胆汁酸转化为次级胆汁酸,从而改变机体内胆汁酸的组成。初级胆汁酸和次级胆汁酸均可调控法尼酯X核受体(FXR)的表达,已有研究发现,FXR在胆汁酸的合成与代谢、葡萄糖代谢、脂质代谢和肝脏自噬中发挥着重要作用。此外,胆汁酸在肠道微生物的互作中起到了重要作用。本文主要综述了胆汁酸与肠道微生物之间的互作及其在宿主代谢调控中的作用,首次探讨了胆汁酸与肠道微生物的互作可能调节自噬相关信号通路。 | Yang-fan NIE Jun HU Xiang-hua YAN | 2015 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2015，16，6： | 19 |
| 11 | Contrast sensitivity and higher-order aberrations in patients with astigmatism显示文摘背景散光是为完成令人满意的视觉功能的最重要的障碍之一。这研究是在对比敏感(CS ) 上评估散光的影响,高顺序的 aberrations.Methods CS,住所反应和波前错误与散光在 113 个病人被测量, 18-36 年。单身者和双目望远镜视觉性能不到四被检验点亮条件:光适应,有瞪视的光适应,暗视力和暗视力与分别地怒视。住所反应作为正常、反常、低被分类。当散光的屈光的力量增加了,到 CS,住所反应和波前错误的散光的力量和轴的贡献是 analyzed.Results, CS 空间频率的损失从高度改变了到中介,然后到低频率。CS 分数在不同照度层次变化了,在下列顺序下降:光适应,有瞪视的光适应,暗视力,和有瞪视的暗视力。然而,正常住所组比没有瞪视与瞪视在光适应下面显示出更好的 CS 价值。当光 accommadation 稀释了时,正弦波浪栅栏的影响方向的范围主要在高屈光的力量的顶点线留下了,它将被扩展。有对称的散光的病人比与主导的眼睛视觉与 binoculus 视觉得到了更高的 CS 分数,当有不均匀的散光的病人与瞪视仅仅在暗视力做了这时。在高顺序的错误,昏迷错误,第二等的昏迷错误和总数之中,更高的顺序错误被散光影响,所有哪个与减少散光的散光 increased.Conclusions 的力量升起可能改进视觉功能的表演。散光的力量应该不仅被砍倒,而且双目望远镜轴应该相称性地被做。 | ZHENG Guang-ying DU Jun ZHANG Jin-song LIU Su-bing NIE Xiao-li ZHU Xiao-hong TANG Xiu-xia XIN Bao-li MAI Zhi-bin ZHANG Wei-xia | 2007 | Chinese Medical Journal2007，，10： | 16 |
| 12 | LYRM03, an ubenimex derivative, attenuates LPS- induced acute lung injury in mice by suppressing the TLR4 signaling pathway显示文摘像使用费的受体(TLR4 ) 4 调停了表明戏在导致败血的尖锐的肺损害(ALI ) 的一个关键角色。LYRM03 (3-amino-2-hydroxy-4-phenyl-valyl-isoleucine ) 是 ubenimex 的新奇衍生物,广泛地使用的 antineoplastic 药。我们以前发现 LYRM03 在盲肠的结扎刺老鼠模型有反煽动性的效果。在这研究,我们决定了 LYRM03 是否在老鼠稀释了导致 LPS 的 ALI。导致 LPS 的 ALI 老鼠模型被与 LPS (5 mg/kg ) 的 intratracheal 注射质问老鼠建立,它随后与 LYRM03 被对待(10 mg/kg, ip ) 。LYRM03 管理显著地减轻了导致 LPS 的肺浮肿,煽动性的房间(neutrophils 和巨噬细胞) 渗入和 myeloperoxidase (军邮局) 活动,减少的支持 inflammatory 和趋化性的 cytokine (TNF- , IL-6, IL-1, MIP-2 ) 产生和在肺纸巾的 COX-2 表示。在有教养的老鼠在 vitro 的牙槽的巨噬细胞,有 LYRM03 (100 mol/L ) 的预告的处理由减少 Myd88 表示,增加 IB 稳定性并且禁止 p38 phosphorylation 压制了导致 LPS 的巨噬细胞激活。这些结果建议 LYRM03 有效地由在牙槽的巨噬细胞禁止支持 inflammatory 调停人和 Myd88 依赖的 TLR4 发信号小径的表示稀释导致 LPS 的 ALI。LYRM03 可以为调停败血的肺损害用作一个潜在的处理。 | Hui-qiong HE Ya-xian WU Yun-juan NIE Jun WANG Mei GE Feng QIAN | 2017 | Acta Pharmacologica Sinica2017，38，3： | 14 |
| 13 | Correlation between TIMP-1 expression and liver fibrosis in two rat liver fibrosis models显示文摘瞄准:在老鼠在导致免疫者、导致 CCL4 的肝纤维变性模型评估在 TIMP-1 表示和肝纤维变性之间的浆液 TIMP-1 水平和关联。方法:导致免疫者、导致 CCL4 的肝纤维变性模型被地塞米松(0.01 mg ) 和 CCL4 分别地建立。浆液 TIMP-1 水平与 ELISA 被检测,当肝活体检视的组织病理学说的等级被评估时。枪兵等级关联测试被用来在二个纤维变性模型分析在 TIMP-1 表示和肝的纤维变性之间的关联的差别。而且,原位杂交被用来在二个模型决定 TIMP-1 mRNA 的表示差别。结果:积极关联在有免疫力的劝诱的组的浆液 TIMP-1 水平和相应老鼠的纤维变性肝的组织病理学说的阶段之间存在(枪兵等级关联测试, r = 0.812, P <
0.05 ) ,并且 TIMP-1 mRNA 的积极原位杂交信号是强壮的。在导致 CCL4 的肝纤维变性模型,在浆液 TIMP-1 水平和肝的纤维变性的严厉之间的关联不是统计上重要的(枪兵等级关联测试, r = 0.229, P >
0.05 ) 。并且与导致免疫者的模型,相比, TIMP-1 mRNA 的积极原位杂交信号是更弱的,当表达式变化在一样的严厉的肝的纤维变性是更高的时。结论:在在二个老鼠肝纤维变性模型的 TIMP-1 表示和肝纤维变性之间的关联是不同的。在导致免疫者的模型,,浆液 TIMP-1 水平能在导致 CCL4 的模型,反映肝纤维变性的严厉在浆液 TIMP-1 水平和肝的纤维变性的严厉之间的关联不是统计上重要的。 | Qing-He Nie Ya-Fei Zhang Yu-Mei Xie Xin-Dong Luo Bin Shao Jun Li Yong-Xing Zhou | 2006 | World Journal of Gastroenterology2006，12，19： | 12 |
| 14 | Molecular Typing of Leptospira interrogans Strains Isolated from Rattus tanezumi in Guizhou Province,Southwest of China显示文摘Objective To identify and type three leptospires isolated from Rattus tanezumi in Guizhou Province by using three molecular techniques (PFGE, MLVA, and MLST), reveal the molecular characteristic of causative agents of local leptospirosis and evaluate these three molecular methods based on their detection resolution and efficiency. Methods Three Leptospira strains were isolated from the kidney of Rattus tanezumi and cultured with EMJH medium. PFGE, MLVA, and MLST assays were applied to type the three strains isolated from Rattus tanezumi in Guizhou Province. Results PFGE, MLVA, and MLST typing showed that the three leptospiral isolates matched with leptospiral serogroup Icterohaemorrhagiae serovar Lai. The findings of the genotyping methods were consistent. MLVA and MLST defined genotypes, whereas PFGE allowed the recognition of additional subgroups within the genotypes, and the findings of molecular typing were also consistent with those of traditional techniques. Conclusion Three leptospiral isolates from Guizhou Province matched with leptospiral serogroup Icterohaemorrhagiae serovar Lai, and PFGE, MLVA, and MLST, as reliable molecular techniques for identifying and typing of Leptospira interrogans, would contribute to the active surveillance, outbreak investigation and source tracking for leptospirosis in Guizhou Province. | LI Shi Jun ZHANG Cui Cai LI Xiu Wen TIAN Ke Cheng TANG Guang Peng WANG Ding Ming LIU Ying NIE Yi Xin JIANG Xiu Gao | 2012 | Biomedical and Environmental Sciences2012，25，5： | 12 |
| 15 | Effects of Film-Degraded Rice Controlled Release Fertilizer on the Nitrogen Uptake and Yield of the Hybrid Rice显示文摘Field experiment was carried out to nitrogen release characteristics of rice controlled release fertilizer (RCRF) coated with natural and half natural high molecular materials, and to thereof effects on the nitrogen uptake and yield of early and late hybrid rice from 1999 to 2000, with urea as control.In experimental fields regardless of early and late seasons, the amount of N in the plants increased exponentially at the early growth stage (y=abx) and nonlinearly at the middle and late growth stages (y=a+bx+cx2).Rice controlled release fertilizer lengthened the exponential phase and increased the parameter -b/c during the nonlinear phase. Although the N amount of plants was less in rice controlled release fertilizer plots than in urea plots within 20 days after transplanting, at the heading and maturity stages, the N amount of plants in rice controlled release fertilizer plots was above two times greater than in the urea plots due to the higher N recovery, and the high absorption density of N in the nonlinear phase. Tillers pattern and achievement of the maximum tillering stage varied with fertilizer types. The maximum tillering stage in the urea plots occurred a week earlier than in the rice controlled release fertilizer plots. Yield sink size and potential sink size of the plants reflected the N absorption pattern and the amount of N in the plants. The yields of early and late rice with 90 kg N ha-1of rice controlled release fertilizer were respectively increased by 832.7 kg ha-1 and 412.8 kg ha-1 than those with 90 kg N ha-1 of urea, almost equivalent to the yield of early and late hybrid rice with 180 kg N ha-1 of urea. | Zheng Shengxian, Nie Jun, Xiao Jian, Xiong Jinying, and Yi Guoying Hunan Soil and Fertilizer Institute, Changsha 410125, China | 2002 | Hunan Agricultural Science & Technology Newsletter2002，3，2： | 12 |
| 16 | Substitution of chemical fertilizer by Chinese milk vetch improves the sustainability of yield and accumulation of soil organic carbon in a double-rice cropping system显示文摘The double-rice cropping system is a very important intensive cropping system for food security in China. There have been few studies of the sustainability of yield and accumulation of soil organic carbon (SOC) in the double-rice cropping system following a partial substitution of chemical fertilizer by Chinese milk vetch (Mv). We conducted a 10-year (2008–2017) field experiment in Nan County, South-Central China, to examine the double-rice productivity and SOC accumulation in a paddy soil in response to different fertilization levels and Mv application (22.5 Mg ha^–1). Fertilizer and Mv were applied both individually and in combination (sole chemical fertilizers, Mv plus 100, 80, 60, 40, and 0% of the recommended dose of chemical fertilizers, labeled as F100, MF100, MF80, MF60, MF40, and MF0, respectively). It was found that the grain yields of double-rice crop in treatments receiving Mv were reduced when the dose of chemical fertilizer was reduced, while the change in SOC stock displayed a double peak curve. The MF100 produced the highest double-rice yield and SOC stock, with the value higher by 13.5 and 26.8% than that in the F100. However, the grain yields increased in the MF80 (by 8.4% compared to the F100), while the SOC stock only increased by 8.4%. Analogous to the change of grain yield, the sustainable yield index (SYI) of double rice were improved significantly in the MF100 and MF80 compared to the F100, while there was a slight increase in the MF60 and MF40. After a certain amount of Mv input (22.5 Mg ha^–1), the carbon sequestration rate was affected by the nutrient input due to the stimulation of microbial biomass. Compared with the MF0, the MF100 and MF40 resulted in a dramatically higher carbon sequestration rate (with the value higher by 71.6 and 70.1%), whereas the MF80 induced a lower carbon sequestration rate with the value lower by 70.1% compared to the MF0. Based on the above results we suggested that Mv could partially replace chemical fertilizers (e.g., 40–60%) to improve or maintain the productivity and sustainability of the double-rice cropping system in South-Central China. | ZHOU Xing LU Yan-hong LIAO Yu-lin ZHU Qi-dong CHENG Hui-dan NIE Xin CAO Wei-dong NIE Jun | 2019 | Journal of Integrative Agriculture2019，18，10： | 11 |
| 17 | Establishment of intestinal organoid cultures modeling injury-associated epithelial regeneration显示文摘The capacity of 3D organoids to mimic physiological tissue organization and functionality has provided an invaluable tool to model development and disease in vitro.However,conventional organoid cultures primarily represent the homeostasis of self-organizing stem cells and their derivatives.Here,we established a novel intestinal organoid culture system composed of 8 components,mainly including VPA,EPZ6438,LDN193189,and R-Spondin 1 conditioned medium,which mimics the gut epithelium regeneration that produces hyperplastic crypts following injury;therefore,these organoids were designated hyperplastic intestinal organoids(Hyper-organoids).Single-cell RNA sequencing identified different regenerative stem cell populations in our Hyper-organoids that shared molecular features with in vivo injury-responsive Lgr5+stem cells or Clu+revival stem cells.Further analysis revealed that VPA and EPZ6438 were indispensable for epigenome reprogramming and regeneration in Hyper-organoids,which functioned through epigenetically regulating YAP signaling.Furthermore,VPA and EPZ6438 synergistically promoted regenerative response in gut upon damage in vivo.In summary,our results demonstrated a new in vitro organoid model to study epithelial regeneration,highlighting the importance of epigenetic reprogramming that pioneers tissue repair. | Molong Qu Liang Xiong Yulin Lyu Xiannian Zhang Jie Shen Jingyang Guan Peiyuan Chai Zhongqing Lin Boyao Nie Cheng Li Jun Xu Hongkui Deng | 2021 | Cell Research2021，31，3： | 9 |
| 18 | Biocompatible and biodegradable nanoparticles for enhancement of anti-cancer activities of phytochemicals显示文摘Many phytochemicals show promise in cancer prevention and treatment, but their low aqueous solubility, poor stability, unfavorable bioavailability, and low target specificity make administering them at therapeutic doses unrealistic. This is particularly true for(-)-epigallocatechin gallate, curcumin, quercetin, resveratrol, and genistein. There is an increasing interest in developing novel delivery strategies for these natural products. Liposomes, micelles, nanoemulsions, solid lipid nanoparticles, nanostructured lipid carriers and poly(lactide-co-glycolide) nanoparticles are biocompatible and biodegradable nanoparticles. Those nanoparticles can increase the stability and solubility of phytochemicals, exhibit a sustained release property, enhance their absorption and bioavailability, protect them from premature enzymatic degradation or metabolism, prolong their circulation time, improve their target specificity to cancer cells or tumors via passive or targeted delivery, lower toxicity or side-effects to normal cells or tissues through preventing them from prematurely interacting with the biological environment, and enhance anti-cancer activities. Nanotechnology opens a door for developing phytochemical-loaded nanoparticles for prevention and treatment of cancer. | LI Chuan ZHANG Jia ZU Yu-Jiao NIE Shu-Fang CAO Jun WANG Qian NIE Shao-Ping DENG Ze-Yuan XIE Ming-Yong WANG Shu | 2015 | Chinese Journal of Natural Medicines2015，13，9： | 8 |
| 19 | Solving 3D Electromagnetic Scattering and Radiation by Local Multilevel Fast Multipole Algorithm显示文摘 | HU Jun NIE Zaiping LEI Lin RUI Xi WANG Jun | 2007 | Chinese Journal of Electronics2007，16，2： | 8 |
| 20 | Research on KOH/La-Ba-Al_2O_3 catalysts for biodiesel production via transesterification from microalgae oil显示文摘Alumina supports modified by lanthanum(La)and barium(Ba)were prepared by peptization.Catalysts with different KOH contents supported on modified alumina were prepared by impregnation method.Various techniques,including N2 adsorption-desorption(Brunauer-Emmet-Teller method,BET),X-ray diffraction(XRD),scanning electron microscopy(SEM),and fourier transform infrared absorption spectroscopy(FT-IR). Catalytic activity for microalgae oil conversion to methyl ester via transesterification was evaluated and analyzed by GC-MS and GC.BET results showed that the support possessed high specific surface area,suitable pore volume and pore size distribution.Activity results indicated that the catalyst with 25 wt%KOH showed the best activity for microalgae oil conversion.XRD and SEM results revealed that Al-O-K compound was the active phase for microalgae oil conversion.The agglomeration and changing of pore structure should be the main reasons for the catalyst deactivation when KOH content was higher than 30 wt%. | Xiaoyu Zhang Qing Ma Bibo Cheng Jun Wang Jinshan Li Fude Nie | 2012 | Journal of Natural Gas Chemistry2012，21，6： | 7 |
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