|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Macrophage secretory products induce an inflammatory phenotype in hepatocytes显示文摘AIM:To investigate the influence of macrophages on hepatocyte phenotype and function.METHODS:Macrophages were differentiated from THP-1 monocytes via phorbol myristate acetate stimulation and the effects of monocyte or macrophageconditioned medium on HepG2 mRNA and protein expression determined.The in vivo relevance of these findings was confirmed using liver biopsies from 147 patients with hepatitis C virus(HCV)infection.RESULTS:Conditioned media from macrophages,but not monocytes,induced a transient morphological change in hepatocytes associated with upregulation of vimentin(7.8±2.5-fold,P=0.045)and transforming growth factor(TGF)-β1(2.6±0.2-fold,P<0.001)and downregulation of epithelial cadherin(1.7±0.02-fold,P=0.017)mRNA expression.Microarray analysis revealed significant upregulation of lipocalin-2(17-fold,P <0.001)and pathways associated with inflammation,and substantial downregulation of pathways related to hepatocyte function.In patients with chronic HCV,realtime polymerase chain reaction and immunohistochemistry confirmed an increase in lipocalin-2 mRNA(F0 1.0 ±0.3,F1 2.2±0.2,F2 3.0±9.3,F3/4 4.0±0.8,P= 0.003)and protein expression(F1 1.0±0.5,F2 1.3± 0.4,F3/4 3.6±0.4,P=0.014)with increasing liver injury.High performance liquid chromatography-tandem mass spectrometry analysis identified elevated levels of matrix metalloproteinase(MMP)-9 in macrophageconditioned medium,and a chemical inhibitor of MMP-9 attenuated the change in morphology and mRNA expression of TGF-β1(2.9±0.2 vs 1.04±0.1,P<0.001) in macrophage-conditioned media treated HepG2 cells.In patients with chronic HCV infection,hepatic mRNA expression of CD163(F0 1.0±0.2,F1/2 2.8±0.3,F3/4 5.3±1.0,P=0.001)and MMP-9(F0 1.0±0.4,F1/2 2.8±0.3,F3/4 4.1±0.8,P=0.011)was significantly associated with increasing stage of fibrosis.CONCLUSION:Secreted macrophage products alter the phenotype and function of hepatocytes,with increased expression of inflammatory mediators,suggesting that hepatocytes actively participate in liver injury. | Michelle Melino Victoria L Gadd Gene V Walker Richard Skoien Helen D Barrie Dinesh Jothimani Leigh Horsfall Alun Jones Matthew J Sweet Gethin P Thomas Andrew D Clouston Julie R Jonsson Elizabeth E Powell | 2012 | World Journal of Gastroenterology2012，18，15： | 3 |
| 2 | Non-invasive assessment of fibrosis in liver grafts due to hepatitis C virus recurrence显示文摘 | JS Cross T Jothimani D Heneghan MA | 2011 | Clin Transplant2011，25，6： | 1 |
| 3 | Long-term immunosuppression for prevention of nonviral disease recurrence显示文摘 | NEUBERGER J JOTHIMANI D | 2005 | Transplant Proc2005，37，4： | 1 |
| 4 | Phytocheniical and pharmacological evaluation of prop roots of Pandanus fascicularis Lam显示文摘Objective:To evaluate the anti-inflammatory and analgesic activities of the ethanol and aqueous extracts of prop roots of Pandanus fascicularis(P.fascicularis) Lam(pandanaceae).And provide experimental evidence for its traditional use such as rheumatoid arthritis and spasmodic. Methods:The anti-inflammatory activity was observed by carrageenan-induced edema of the hind paw of rats.Analgesic activities of prop roots of P.fascicularis were determined using acetic acid induced writhing model and tail clip method in mice and rat,respectively.The ethanol fraction was then subjected to chromatographic analysis and a compound has been isolated and characterized by IR,~1H-NMR and mass spectroscopy.Results:Edema suppressant effect of ethanol extract was found to be 37.03%inhibition whereas aqueous extract was found to be 63.22%inhibition after 3 h which was nearly equivalent to that of 10 mg/kg of indomethacin (67.81%).Percentage inhibition of writhing compared to control were 63.15%,54.38%,14.90%for aspirin,aqueous extract and ethanolic extract,respectively.Both ethanol and aqueous extracts show significant activity against appropriate controls after 60 min of treatment on tail clip method. The structure of the isolated compound is may be characterized as Hepta deca-5-ene-l-ol by analysis it’s IR,H-NMR and mass spectroscopy data.Conclusions:The extracts of prop roots of P.fascicularis produce significant analgesic and anti-inflammatory activities,supporting the traditional application of this herb in treating various diseases associated with inflammation and pain. | Jothimani Rajeswari Karthikeyan Kesavan Balasundaram Jayakar | 2011 | Asian Pacific Journal of Tropical Medicine2011，4，8： | 1 |
| 5 | Anaerobic biodegradation of aromatic compounds显示文摘 | Jothimani P Kalaichelvan G Bhaskaran A | 2003 | Indian Journal of Experimental Biology2003，41，： | 1 |
| 6 | Magnetic resonance imaging and spectroscopy accurately estimate the severity of steatosis provided the stage of fibrosis is considered<.--'Genetic and Metabolic Liver Disease' -->显示文摘 | Stuart McPherson Julie R. Jonsson Gary J. Cowin Peter O’Rourke Andrew D. Clouston Andrew Volp Leigh Horsfall Dinesh Jothimani Jonathan Fawcett Graham J. Galloway Mark Benson Elizabeth E. Powell | 2009 | Journal of Hepatology2009，，2： | 1 |
| 7 | Post-COVID-19 cholangiopathy:Current understanding and management options显示文摘Post-coronavirus disease 2019(COVID-19)cholangiopathy(PCC)is a rare but lifethreatening complication of COVID-19 infection.PCC typically presents when patients recovering from the contagion and manifests as cholestasis in patients with no history of pre-existing liver disease.The pathogenesis of PCC is little understood.Hepatic injury in PCC could be mediated by the predilection of severe acute respiratory syndrome coronavirus 2 for cholangiocytes.Though PCC shows some resemblance to secondary sclerosing cholangitis in critically ill patients,it is considered as a separate and unique entity in the literature.Various treatment options like ursodeoxycholic acid,steroids,plasmapheresis,and endoscopic retrograde cholangiopancreatography guided interventions have been tried but with limited success.We have noticed significant improvement in liver function with antiplatelet therapy in a couple of patients.PCC can progress to end-stage liver disease necessitating liver transplantation.In this article,we discuss the current knowledge of PCC focusing on its pathophysiology,clinical manifestations,and management strategies. | Fadl H Veerankutty Kushan Sengupta Mukul Vij Ashwin Rammohan Dinesh Jothimani Ananthavadivelu Murali Mohamed Rela | 2023 | World Journal of Gastrointestinal Surgery2023，15，5： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员