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| 1 | Rapid assembly of multilayer microfluidic structures via 3D-printed transfer molding and bonding显示文摘A critical feature of state-of-the-art microfluidic technologies is the ability to fabricate multilayer structures without relying on the expensive equipment and facilities required by soft lithography-defined processes.Here,three-dimensional(3D)printed polymer molds are used to construct multilayer poly(dimethylsiloxane)(PDMS)devices by employing unique molding,bonding,alignment,and rapid assembly processes.Specifically,a novel single-layer,two-sided molding method is developed to realize two channel levels,non-planar membranes/valves,vertical interconnects(vias)between channel levels,and integrated inlet/outlet ports for fast linkages to external fluidic systems.As a demonstration,a single-layer membrane microvalve is constructed and tested by applying various gate pressures under parametric variation of source pressure,illustrating a high degree of flow rate control.In addition,multilayer structures are fabricated through an intralayer bonding procedure that uses custom 3D-printed stamps to selectively apply uncured liquid PDMS adhesive only to bonding interfaces without clogging fluidic channels.Using integrated alignment marks to accurately position both stamps and individual layers,this technique is demonstrated by rapidly assembling a six-layer microfluidic device.By combining the versatility of 3D printing while retaining the favorable mechanical and biological properties of PDMS,this work can potentially open up a new class of manufacturing techniques for multilayer microfluidic systems. | Casey C.Glick Mitchell T.Srimongkol Aaron J.Schwartz William S.Zhuang Joseph C.Lin Roseanne H.Warren Dennis R.Tekell Panitan A.Satamalee Liwei Lin | 2016 | Microsystems & Nanoengineering2016，2，1： | 4 |
| 2 | Microcosm investigations of stormwater pond sediment toxicity to embryonic and larval amphibians:variation in sensitivity among species显示文摘 | Snodgrass J W Casey R E Joseph D Simon J A | | 0，，02： | 1 |
| 3 | Three-Dimensional Model for the Human Cl ? /HCO 3 ? Exchanger, AE1, by Homology to the E. coli ClC Protein显示文摘 | Pamela Bonar Hans-Peter Schneider Holger M. Becker Joachim W. Deitmer Joseph R. Casey | 2013 | Journal of Molecular Biology2013，，14： | 1 |
| 4 | Transcriptomic Profiling of Human Pluripotent Stem Cell-derived Retinal Pigment Epithelium over Time显示文摘Human pluripotent stem cell(h PSC)-derived progenies are immature versions of cells,presenting a potential limitation to the accurate modelling of diseases associated with maturity or age.Hence,it is important to characterise how closely cells used in culture resemble their native counterparts.In order to select appropriate time points of retinal pigment epithelium(RPE)cultures that reflect native counterparts,we characterised the transcriptomic profiles of the h PSC-derived RPE cells from 1-and 12-month cultures.We differentiated the human embryonic stem cell line H9 into RPE cells,performed single-cell RNA-sequencing of a total of 16,576 cells to assess themolecular changes of the RPE cells across these two culture time points.Our results indicate the stability of the RPE transcriptomic signature,with no evidence of an epithelial–mesenchymal transition,and with the maturing populations of the RPE observed with time in culture.Assessment of Gene Ontology pathways revealed that as the cultures age,RPE cells upregulate expression of genes involved in metal binding and antioxidant functions.This might reflect an increased ability to handle oxidative stress as cells mature.Comparison with native human RPE data confirms a maturing transcriptional profile of RPE cells in culture.These results suggest that long-term in vitro culture of RPE cells allows the modelling of specific phenotypes observed in native mature tissues.Our work highlights the transcriptional landscape of h PSC-derived RPE cells as they age in culture,which provides a reference for native and patient samples to be benchmarked against. | Grace E.Lidgerwood Anne Senabouth Casey J.A.Smith-Anttila Vikkitharan Gnanasambandapillai Dominik C.Kaczorowski Daniela Amann-Zalcenstein Erica L.Fletcher Shalin H.Naik Alex W.Hewitt Joseph E.Powell Alice Pebay | 2021 | Genomics, Proteomics & Bioinformatics2021，19，2： | 1 |
| 5 | Enhanced external counterpulsation improves endothelial function and exercise capacity in patients with ischaemic left ventricular dysfunction显示文摘 | Darren T Beck Jeffrey S Martin Darren P Casey Joseph C Avery Paloma D Sardina Randy W Braith | 2014 | Clin Exp Pharmacol Physiol2014，，： | 1 |
| 6 | Cellular trac- tion stresses increase with increasing metastatic potential 显示文摘 | Kraning-Rush Casey M Joseph P Reinhart-King C A | 2012 | PLoS ONE2012，7，32： | 1 |
| 7 | Microcosm investigations of stormwater pond sediment toxicity to embryonic and larval amphibians: variation in sensitivity among species 显示文摘 | SNODGRASS J W CASEY R E JOSEPH D | 2008 | Environ Pollut2008，154，2： | 1 |
| 8 | Ethical Dilemma of Mandated Con- traception in Pharmaceutical Research at Catholic Medical Institutions 显示文摘 | Murray Joseph Casey Richard O' Brien Marc Rendell | 2012 | The American Journal of Bioethics2012，12，7： | 1 |
| 9 | Antibody-Based Immunotherapy of Cancer显示文摘 | Louis M. Weiner Joseph C. Murray Casey W. Shuptrine | 2012 | Cell2012，，6： | 1 |
| 10 | Intra-abdominal carcinomatosis after prophylactic oophorectomy in women of hereditary breast ovarian cancer syndrome kindreds associated with BRCA1 and BRCA2 mutations显示文摘 | Murray Joseph Casey Carrie Synder Chhanda Bewtra Steven A. Narod Patrice Watson Henry T. Lynch | 2005 | Gynecologic Oncology2005，，2： | 1 |
| 11 | The use of feed-grade amino acids in lactating sow diets显示文摘Background: The use of feed grade amino acids can reduce the cost of lactation feed. With changing genetics,increasing feed costs, and higher number of pigs weaned with heavier wean weights further evaluation of higher inclusion levels of feed-grade amino acid in lactation diets than previously published is warranted. Two experiments(Exp.) were conducted to determine the optimal inclusion level of L-lysine HCl to be included in swine lactation diets while digestible lysine levels remain constant across dietary treatments and allowing feed grade amino acids to be added to the diet to maintain dietary ratios relative to lysine to maximize litter growth rate and sow reproductive performance. Furthermore, the studies were to evaluate minimal amino acid ratios relative to lysine that allows for optimal litter growth rate and sow reproductive performance.Results: Exp. 1: Increasing L-lysine HCl resulted in similar gilt feed intake, litter, and reproductive performance.Average litter gain from birth to weaning was 2.51, 2.49, 2.59, 2.43, and 2.65 kg/d when gilts were fed 0.00, 0.075,0.150, 0.225, and 0.30% L-lysine HCl, respectively. Exp. 2: The average litter gain from birth to weaning was 2.68,2.73, 2.67, 2.70, and 2.64 kg/d(P < 0.70) when sows were fed 0.1, 0.2, 0.3, 0.4, and 0.4% L-lysine HCl plus valine,respectively. No other differences among dietary treatments were observed.Conclusions: Collectively, these studies demonstrate corn-soybean meal based lactation diets formulated with a constant SID lysine content for all parities containing up to 0.40% L-lysine HCl with only supplemental feed grade threonine and a methionine source have no detrimental effect on litter growth rate and subsequent total born. | Laura Greiner Pairat Srichana James L.Usry Casey Neill Gary L.Allee Joseph Connor Kevin J.Touchette Christopher D.Knight | 2018 | Journal of Animal Science and Biotechnology2018，9，2： | 0 |
| 12 | AB087.Corneal phenotype of a Slc4a11 knockout murine model for congenital hereditary endothelial dystrophy显示文摘Background:Congenital hereditary endothelial dystrophy(CHED)is characterized by blindness at birth or in early infancy resulting from bilateral corneal opacification,and is linked to mutation in the Slc4a11 gene.A Slc4a11 knockout(KO)mouse,generated by gene deletion(Vithana et al.Nat Genet 2006),was acquired in order to study this disease.To confirm the phenotype of this Slc4a11 KO mouse model as a function of age,using the wild type(WT)mouse as a control.Methods:Genotyping was performed by PCR(REDExtract-N-AmpTM Tissue PCR Kit,Sigma-Aldrich,Oakville,ON).Slc4a11 WT and KO mice populations aged from 5 to 50 weeks were studied(n=5 animals per age group;5-year age intervals).Slit lamp examination,anterior segment-ocular coherence tomography(OCT930SR;Thorlabs,Inc.,Newton,NJ),corneal endothelial cell staining,and scanning(SEM)and transmission(TEM)electron microscopy were used to assess the morphological and cellular differences between the two groups.The expression of basolateral membrane transporter NaBC1 within the corneal endothelium was also assessed using immunohistochemistry.Results:Diffuse and progressive corneal opacification was observed at the slit lamp in the Slc4a11 KO mice,starting at 10 weeks.The central corneal thickness(CCT)also increased progressively as a function of time.In comparison,Slc4a11 WT corneas remained clear over the entire study period.Early TEM results showed vacuole degeneration of the corneal endothelium in the 15-week KO mouse,which was not seen in the same age WT mouse.Conclusions:The corneal phenotype of this Slc4a11 KO mouse is representative of the clinical manifestations of CHED in human subjects,confirming the usefulness of this model for studying pathophysiology and therapeutic alternatives for Slc4a11-associated corneal dystrophies. | Sergiu Vlad Antoine Sylvestre-Bouchard Hasitha Alwis Weerasekera Rami Darwich Marilyse Piché Khampoun Sayasith Joseph Casey Isabelle Brunette | 2018 | Annals of Eye Science2018，，1： | 0 |
| 13 | Physical contributors to glenohumeral internal rotation deficit in high school baseball players显示文摘背景对棒球运动员而言,盂肱关节内旋能力降低(GIRD)是导致肩部和肘部损伤的一个危险因素。尽管这为推荐拉伸提供证据基础,但内旋活动范围(ROM)临床测试无法区分GIRD是由于肌肉韧带还是骨成分因素导致。因此正确理解导致GIRD的原因,对采取针对性干预措施有重要意义。本研究假设骨成分是导致其内旋能力降低的主要因素,其次是肌肉僵硬和后囊膜厚度。方法以156位棒球运动员为对象,评估其内旋ROM,肌肉僵硬(小圆肌、冈下肌与三角肌后束)程度,后囊膜厚度和肱骨后移程度。每个变量都计算了左右侧的差异。将变量输入多元线性回归分析以确定GIRD的重要预测因素。结果:该回归模型只在肱骨后移作为重要预测因素(β=-0 243,t_(156)=-4 9,p<0 01)时存在显著性差异(R^2=0 134,F(1,1 56)=24 0,p<0 01)。不同侧肱骨后移的变化与GIRD有关。结论:肱骨后移在导致GIRD的原因中占13 3%。而浅表肌肉和囊膜僵硬不能作为GIRD的预测因素。在本研究中未评估的因素,如更深层肌肉的僵硬程度、囊膜GIRD或韧带松弛以及神经肌肉调节能力迟缓也可能导致GIRD。肱骨后移是导致GIRD的最大的因素,需要确认其原因。骨成分在GIRD中仅占13 3%,揭示了在这项研究中并未解决软组织因素对该问题的影响。需要进一步确认这些因素,以便完善其评估证据及干预方案,从而降低棒球运动员的受伤风险度。 | Elizabeth E. Hibberd Casey E. Shutt Sakiko Oyama J. Troy Blackburn Joseph B. Myers | 2015 | Journal of Sport and Health Science2015，4，3： | 0 |
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