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| 1 | Adenovirus-mediated gene delivery:Potential applications for gene and cell-based therapies in the new era of personalized medicine显示文摘With rapid advances in understanding molecular pathogenesis of human diseases in the era of genome sciences and systems biology,it is anticipated that increasing numbers of therapeutic genes or targets will become available for targeted therapies.Despite numerous setbacks,efficacious gene and/or cell-based therapies still hold the great promise to revolutionize the clinical management of human diseases.It is wildly recognized that poor gene delivery is the limiting factor for most in vivo gene therapies.There has been a long-lasting interest in using viral vectors,especially adenoviral vectors,to deliver therapeutic genes for the past two decades.Among all currently available viral vectors,adenovirus is the most efficient gene delivery system in a broad range of cell and tissue types.The applications of adenoviral vectors in gene delivery have greatly increased in number and efficiency since their initial development.In fact,among over 2000 gene therapy clinical trials approved worldwide since 1989,a significant portion of the trials have utilized adenoviral vectors.This review aims to provide a comprehensive overview on the characteristics of adenoviral vectors,including adenoviral biology,approaches to engineering adenoviral vectors,and their applications in clinical and preclinical studies with an emphasis in the areas of cancer treatment,vaccination and regenerative medicine.Current challenges and future directions regarding the use of adenoviral vectors are also discussed.It is expected that the continued improvements in adenoviral vectors should provide great opportunities for cell and gene therapies to live up to its enormous potential in personalized medicine. | Cody S.Lee Elliot S.Bishop Ruyi Zhang Xinyi Yu Evan M.Farina Shujuan Yan Chen Zhao Zongyue Zeng Yi Shu Xingye Wu Jiayan Lei Yasha Li Wenwen Zhang Chao Yang Ke Wu Ying Wu Sherwin Ho Aravind Athiviraham Michael J.Lee Jennifer Moriatis Wolf Russell R.Reid Tong-Chuan He | 2017 | Genes & Diseases2017，4，2： | 19 |
| 2 | Establishment and functional characterization of the reversibly immortalized mouse glomerular podocytes(imPODs)显示文摘Glomerular podocytes are highly specialized epithelial cells and play an essential role in establishing the selective permeability of the glomerular filtration barrier of kidney.Maintaining the viability and structural integrity of podocytes is critical to the clinical management of glomerular diseases,which requires a thorough understanding of podocyte cell biology.As mature podocytes lose proliferative capacity,a conditionally SV40 mutant tsA58-immortalized mouse podocyte line(designated as tsPC)was established from the Immortomouse over 20 years ago.However,the utility of the tsPC cells is hampered by the practical inconvenience of culturing these cells.In this study,we establish a user-friendly and reversibly-immortalized mouse podocyte line(designated as imPOD),on the basis of the tsPC cells by stably expressing the wildtype SV40 T-antigen,which is flanked with FRT sites.We show the imPOD cells exhibit long-term high proliferative activity,which can be effectively reversed by FLP recombinase.The imPOD cells express most podocyte-related markers,including WT-1,Nephrin,Tubulin and Vinculin,but not differentiation marker Synaptopodin.The imPOD cells do not form tumor-like masses in vivo.We further demonstrate that TGFb1 induces a podocyte injury-like response in the FLP-reverted imPOD cells by suppressing the expression of slit diaphragm-associated proteins P-Cadherin and ZO-1 and upregulating the expression of mesenchymal markers,a-SMA,Vimentin and Nestin,as well as fibrogenic factors CTGF and Col1a1.Collectively,our results strongly demonstrate that the newly engineered im-POD cells should be a valuable tool to study podocyte biology both under normal and under pathological conditions. | Xinyi Yu Liqun Chen Ke Wu Shujuan Yan Ruyi Zhang Chen Zhao Zongyue Zeng Yi Shu Shifeng Huang Jiayan Lei Xiaojuan Ji Chengfu Yuan Linghuan Zhang Yixiao Feng Wei Liu Bo Huang Bo Zhang Wenping Luo Xi Wang Bo Liu Rex C.Haydon Hue H.Luu Tong-Chuan He Hua Gan | 2018 | Genes & Diseases2018，5，2： | 5 |
| 3 | CRISPR-Cas9-based genome-wide screening identified novel targets for treating sorafenib-resistant hepatocellular carcinoma:a cross-talk between FGF21 and the NRF2 pathway显示文摘The treatment of hepatocellular carcinoma(HCC)has been dominated by multikinase inhibitors for more than a decade.However,drug resistance can severely restrict the efficacy of these drugs.Using CRISPR/CAS9 genome library screening,we evaluated Kelch-like ECH-associated protein 1(KEAP1)as a key regulator of sorafenib’s susceptibility in HCC.We also investigated whether KEAP1’s knockdown can stabilize nuclear factor(erythroid-derived 2)-like 2(NRF2)protein levels that led to sorafenib’s resistance,including an NRF2 inhibitor that can synergize with sorafenib to abolish HCC’s growth in vitro and in vivo.Furthermore,we clarified that fibroblast growth factor 21(FGF21)is an important downstream regulator of NRF2 in HCC.Intriguingly,we observed that FGF21 bound to NRF2 through the C-terminus of FGF21,thereby stabilizing NRF2 by reducing its ubiquitination and generating a positive feedback loop in sorafenib-resistant HCC.These findings,therefore,propose that targeting FGF21 is a promising strategy to combat HCC sorafenib’s resistance. | Jiang Chen Shi Jiang Huijiang Shao Bixia Li Tong Ji Daniel Staiculescu Jiayan He Jie Zhao Liuxin Cai Xiao Liang Junjie Xu Xiujun Cai | 2022 | Science China(Life Sciences)2022，65，10： | 4 |
| 4 | The development of a sensitive fluorescent protein-based transcript reporter for high throughput screening of negative modulators of lncRNAs显示文摘While the human genome is pervasively transcribed,<2%of the human genome is transcribed into protein-coding mRNAs,leaving most of the transcripts as noncoding RNAs,such as microRNAs and long-noncoding RNAs(lncRNAs),which are critical components of epigenetic regulation.lncRNAs are emerging as critical regulators of gene expression and genomic stability.However,it remains largely unknown about how lncRNAs are regulated.Here,we develop a highly sensitive and dynamic reporter that allows us to identify and/or monitor negative modulators of lncRNA transcript levels in a high throughput fashion.Specifically,we engineer a fluorescent fusion protein by fusing three copies of the PEST destruction domain of mouse ornithine decarboxylase(MODC)to the C-terminal end of the codon-optimized bilirubin-inducible fluorescent protein,designated as dBiFP,and show that the dBiFP protein is highly destabilized,compared with the commonly-used eGFP protein.We further demonstrate that the dBiFP signal is effectively down-regulated when the dBiFP and mouse lncRNA H19 chimeric transcript is silenced by mouse H19-specific siRNAs.Therefore,our results strongly suggest that the dBiFP fusion protein may serve as a sensitive and dynamic transcript reporter to monitor the inhibition of lncRNAs by microRNAs,synthetic regulatory RNA molecules,RNA binding proteins,and/or small molecule inhibitors so that novel and efficacious inhibitors targeting the epigenetic circuit can be discovered to treat human diseases such as cancer and other chronic disorders. | Zongyue Zeng Bo Huang Shifeng Huang Ruyi Zhang Shujuan Yan Xinyi Yu Yi Shu Chen Zhao Jiayan Lei Wenwen Zhang Chao Yang Ke Wu Ying Wu Liping An Xiaojuan Ji Cheng Gong Chengfu Yuan Linghuan Zhang Wei Liu Yixiao Feng Bo Zhang Zhengyu Dai Yi Shen Xi Wang Wenping Luo Rex C.Haydon Hue H.Luu Lan Zhou Russell R.Reid Tong-Chuan He Xingye Wu | 2018 | Genes & Diseases2018，5，1： | 4 |
| 5 | Photothermal treatment with EGFRmAb-AuNPs induces apoptosis in hypopharyngeal carcinoma cells via PI3K/AKT/mTOR and DNA damage response pathways显示文摘 | Yingying Zhang Linhai Cong Jiayan He Yu Wang Yingying Zou Zhihong Yang Yuexin Hu Shiwen Zhang Xiaoguang He | 2018 | Acta Biochimica et Biophysica Sinica2018，50，6： | 2 |
| 6 | Genomic Convergence in the Adaptation to Extreme Environments显示文摘Convergent evolution is especially common in plants that have independently adapted to the same extreme environments(i.e.,extremophile plants).The recent burst of omics data has alleviated many limitations that have hampered molecular convergence studies of non-model extremophile plants.In this review,we summarize cases of genomic convergence in these taxa to examine the extent and type of genomic convergence during the process of adaptation to extreme environments.Despite being well studied by candidate gene approaches,convergent evolution at individual sites is rare and often has a high false-positive rate when assessed in whole genomes.By contrast,genomic convergence at higher genetic levels has been detected during adaptation to the same extreme environments.Examples include the convergence of biological pathways and changes in gene expression,gene copy number,amino acid usage,and GC content.Higher convergence levels play important roles in the adaptive evolution of extremophiles and may be more frequent and involve more genes.In several cases,multiple types of convergence events have been found to co-occur.However,empirical and theoretical studies of this higher level convergent evolution are still limited.In conclusion,both the development of powerful approaches and the detection of convergence at various genetic levels are needed to further reveal the genetic mechanisms of plant adaptation to extreme environments. | Shaohua Xu Jiayan Wang Zixiao Guo Ziwen He Suhua Shi | 2020 | Plant Communications2020，1，6： | 1 |
| 7 | TwoMADS-boxgenes regulate vascularcambium activity and secondary growth by modulating auxin homeostasis in Populus显示文摘In trees,stem secondary growth depends on vascular cambium proliferation activity and subsequent cell differentiation,in which an auxin concentration gradient across the cambium area plays a crucial role in regulating the process.However,the underlying molecular mechanismfor the establishment of auxin concentration is not fully understood.In this study,we identified two function-unknown MADS-box genes,VCM1 and VCM2,which are expressed specifically in the vascular cambium and modulate the subcellular homeostasis of auxin.Simultaneous knockdown of both VCM1 and VCM2 enhanced vascular cambium proliferation activity and subsequent xylem differentiation.Overexpression of VCM1 suppressed vascular cambium activity and wood formation by regulating PIN5 expression,which tuned the soluble auxin concentration in the vascular cambium area.This study reveals the role of VCM1 and VCM2 in regulating the proliferation activity of the vascular cambium and secondary growth by modulating the subcellular auxin homeostasis in Populus. | Shuai Zheng Jiajia He Zengshun Lin Yingying Zhu Jiayan Sun Laigeng Li | 2021 | Plant Communications2021，2，5： | 1 |
| 8 | Drift-free localisation using prior cross-source map for indoor low-light environments显示文摘In this study,a localisation system without cumulative errors is proposed.First,depth odometry is achieved only by using the depth information from the depth camera.Then the point cloud cross-source map registration is realised by 3D particle filtering to obtain the pose of the point cloud relative to the map.Furthermore,we fuse the odometry results with the point cloud to map registration results,so the system can operate effectively even if the map is incomplete.The effectiveness of the system for long-term localisation,localisation in the incomplete map,and localisation in low light through multiple experiments on the self-recorded dataset is demonstrated.Compared with other methods,the results are better than theirs and achieve high indoor localisation accuracy. | Junyi Tao Weichen Dai Da Kong Jiayan Wan Bin He Yu Zhang | 2023 | IET Cyber-Systems and Robotics2023，5，1： | 0 |
| 9 | A novel MYCN-YTHDF1 cascade contributes to retinoblastoma tumor growth by eliciting m^(6)A-dependent activation of multiple oncogenes显示文摘Retinoblastoma, the most prevalent primary intraocular tumor in children, leads to vision impairment, disability and even death.In addition to RB1 inactivation, MYCN activation has been documented as another common oncogenic alteration in retinoblastoma and represents one of the high-risk molecular subtypes of retinoblastoma. However, how MYCN contributes to the progression of retinoblastoma is still incompletely understood. Here, we report that MYCN upregulates YTHDF1, which encodes one of the reader proteins for N6-methyladenosine(m^(6)A) RNA modification, in retinoblastoma. We further found that this MYCN-upregulated m^(6)A reader functions to promote retinoblastoma cell proliferation and tumor growth in an m^(6)A bindingdependent manner. Mechanistically, YTHDF1 promotes the expression of multiple oncogenes by binding to their m RNAs and enhancing m RNA stability and translation in retinoblastoma cells. Taken together, our findings reveal a novel MYCN-YTHDF1regulatory cascade in controlling retinoblastoma cell proliferation and tumor growth, pinpointing an unprecedented mechanism for MYCN amplification and/or activation to promote retinoblastoma progression. | Yingxiu Luo Mengjia He Jie Yang Feifei Zhang Jie Chen Xuyang Wen Jiayan Fan Xianqun Fan Peiwei Chai Renbing Jia | 2023 | Science China(Life Sciences)2023，66，9： | 0 |
| 10 | Influences of Lactiplantibacillus plantarum and Saccharomyces cerevisiae fermentation on the nutritional components,flavor property and lipid-lowering effect of highland barley显示文摘Highland barley is a well-known cereal in Qinghai-Tibet Plateau area with high nutritional value,which has been reported to be a health-promoting grain for the obesity and the diabetes.Fermentation by certain microbiota can improve the flavor property and nutritional characteristics.In the present study,Lactiplantibacillus plantarum and Saccharomyces cerevisiae were singly or jointly applied to ferment highland barley,and the profile of volatile substances and lipid-lowering effects of the respective extracts were analyzed.Results indicated that either L.plantarum or S.cerevisiae or co-fermentation could consume the polysaccharides of highland barley to provide energy,and dramatically increase the contents of total protein and polyphenol.Gas chromatography-mass spectrometry(GC-MS)analysis revealed that the presence of S.cerevisiae was critical for production of the pleasant flavors,especially for the ethyl ester substances including hexadecanoic acid ethyl,hexanoic acid ethyl ester and so on.Meanwhile,we found that fermented highland barley extracts by L.plantarum exhibited stronger lipid-lowering effects in Caenorhabditis elegans than that by S.cerevisiae,while the co-fermentation not only emitted pleasant odors but also exerted high hypolipidemic function.In all,co-fermentation by L.plantarum and S.cerevisiae was proposed to be a promising processing to improve the flavor and functional properties of highland barley. | Juan Bai Linzhao He Jinfu Zhang Xiangyue Gu Beiqi Wu Anlin Wang Ying Zhu Jiayan Zhang Yansheng Zhao Jie Yuan Xiang Xiao | 2024 | Journal of Future Foods2024，4，3： | 0 |
| 11 | Crystallographic engineering of Zn anodes for aqueous batteries显示文摘With their intrinsic safety and environmental benignity,aqueous Zn-ion batteries(ZIBs)have been considered the most appropriate candidates for replacing alkali metal systems.However,polycrystalline Zn anodes in aqueous environments still pose enormous issues,such as dendrite growth and side reactions.Although many efforts have been made to address these obstacles through interphase modification and electrolyte design,researchers have not been able to improve the inherent thermodynamic stability and ion deposition behavior of the Zn anode.It is imperative to understand and explore advanced anode construction methods from the perspective of crystallinity.This review delves into the feasibility of precisely regulating the crystallographic features of metallic zinc,examines the challenges and merits of reported strategies for fabricating textured zinc,and offers constructive suggestions for the large-scale production and commercial application of aqueous ZIBs. | Shuang Wu Zhenglin Hu Pan He Lingxiao Ren Jiaxing Huang Jiayan Luo | 2023 | eScience2023，3，3： | 0 |
| 12 | Silver-Mediated N-Trifluoromethylation of Amides and Peptides显示文摘Summary of main observation and conclusion We report herein the direct N-trifluoromethylation of N-H amides.Promoted by AgOTf and 2-fluoro-pyridine,the reaction of a variety of amides with Selectfluor,TMSCF3 and CsF proceeds smoothly at room temperature leading to the corresponding N-trifluoromethylated products in satisfactory yields.The protocol is also applicable to amino acid derivatives,resulting in efficient and chemoselective W-trifluoromethylation of di-and tri-peptides with retention of configuration.A mechanism involving reductive elimination of Ag(Ⅲ)intermediates to form N—CF3 bonds is proposed. | Zhenzhen Zhang Jiayan He Lin Zhu Haiwen Xiao Yewen Fang Chaozhong Li | 2020 | Chinese Journal of Chemistry2020，38，9： | 0 |
| 13 | Corrigendum to “The development of a sensitive fluorescent protein-based transcript reporter for high throughput screening of negative modulators of lncRNAs” [Genes & Diseases 5 (2018) 62–74]显示文摘The authors regret having an image assembly error in Figure 5Ca,in which the image for the 'Oh dBiFP-AdRFp'group was erroneously duplicated with an overlapping image from the'36h BiFP dBIFP-AdR-simH19'group.We confirm the error is restricted to the image assembly,and the underlying data and conclusions are correct and unchanged.The authors would like to apologize for any inconvenience caused. | Zongyue Zeng Bo Huang Shifeng Huang Ruyi Zhang Shujuan Yan Xinyi Yu Yi Shu Chen Zhao Jiayan Lei Wenwen Zhang Chao Yang Ke Wu Ying Wu Liping An Xiaojuan Ji Cheng Gong Chengfu Yuan Linghuan Zhang Wei Liu Yixiao Feng Bo Zhang Zhengyu Dai Yi Shen Xi Wang Wenping Luo Rex C. Haydon Hue H. Luu Lan Zhou Russell R. Reid Tong-Chuan He Xingye Wu | 2023 | Genes & Diseases2023，10，2： | 0 |
| 14 | Corrigendum to “Establishment and functional characterization of the reversibly immortalized mouse glomerular podocytes (imPODs)” [Genes & Diseases 5 (2018) 137–149]显示文摘The authors regret having an image assembly error in Figure 3A,in which the image for 'imPOD Synaptopodin DAPl stain'groupwas erroneouslyduplicatedwiththe imagefrom the'tsPOD-33C SynaptopodinDAPIstain'group.We confirm the error is restricted to the image assembly,and the underlying data and conclusions are correct and unchanged.The authors would like to apologize for any inconvenience caused. | Xinyi Yu Liqun Chen Ke Wu Shujuan Yan Ruyi Zhang Chen Zhao Zongyue Zeng Yi Shu Shifeng Huang Jiayan Lei Xiaojuan Ji Chengfu Yuan Linghuan Zhang Yixiao Feng Wei Liu Bo Huang Bo Zhang Wenping Luo Xi Wang Bo Liu Rex C. Haydon Hue H. Luu Tong-Chuan He Hua Gan | 2023 | Genes & Diseases2023，10，2： | 0 |
| 15 | Effects of Polysaccharides from Enteromorpha prolifera on Hepatorenal Syndrome in Mice显示文摘[Objectives]This study was conducted to evaluate the protective effect of polysaccharides from Enteromorpha prolifera(PEP)on mice with hepatorenal syndrome induced by carbon tetrachloride.[Methods]A mouse hepatorenal syndrome model was induced by carbon tetrachloride.The serum levels of lipid,total antioxidant capacity,liver and kidney function,pathological changes of liver and kidney were selected to clarity the effectiveness of PEP on hepatorenal syndrome in mice.[Results]PEP effectively lowered the serum levels of lipid,increased total antioxidant capacity,improved liver and kidney injury,and alleviated pathological changes of liver and kidney of mice induced by carbon tetrachloride.[Conclusions]PEP has a potent preventive effect on hepatorenal syndrome induced by carbon tetrachloride in mice,which provides theoretical support for future clinical application of PEP. | Shanshan WANG Jiayan ZHOU Yue HE Yating ZHOU Qiuxi YANG Qianqian YUAN Qingying DU Hanchi CHENG Suduo JIANG Xiaojuan ZHAO | 2023 | Medicinal Plant2023，14，1： | 0 |
| 16 | From lab to market:a review of commercialization and advances for binders in lithium-,zinc-,sodium-ion batteries显示文摘The paper discusses the progress and commercialization of binders for energy storage applications,such as batteries.It explains the role of binders in holding together active materials and current collectors,and highlights the challenges associated with conventional organic solvents in binders.The potential of aqueous binders is introduced as a cost-effective and environmentally friendly alternative.The advantages and limitations of different types of binders are discussed,and the importance of binder selection for optimal battery performance is emphasized.The current state of commercialization of binders is reviewed,and the need for collaboration between researchers,manufacturers,and policymakers to develop and promote environmentally friendly and cost-effective binders is emphasized.The paper concludes by outlining future directions for research and development to further improve the performance and commercialization of binders,while addressing limitations such as lack of standardization,high cost,and long-term stability and reliability. | Chang Su Xuan Gao Kejiang Liu Yuhang Dai Haobo Dong Yiyang Liu Jiayan Zhu Qiuxia Zhang Hongzhen He Guanjie He | 2024 | Nano Research Energy2024，3，1： | 0 |
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