|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | PCDH17 increases the sensitivity of colorectal cancer to 5-fluorouracil treatment by inducing apoptosis and autophagic cell death显示文摘5-Fluorouracil(5-FU)is known as a first-line chemotherapeutic agent against colorectal cancer(CRC),but drug resistance occurs frequently and significantly limits its clinical success.Our previous study showed that the protocadherin 17(PCDH17)gene was frequently methylated and functioned as a tumor suppressor in CRC.However,the relationship between PCDH17 and 5-FU resistance in CRC remains unclear.Here,we revealed that PCDH17 was more highly expressed in 5-FU-sensitive CRC tissues than in 5-FU-resistant CRC tissues,and high expression of PCDH17 was correlated with high BECN1 expression.Moreover,this expression profile contributed to superior prognosis and increased survival in CRC patients.Restoring PCDH17 expression augmented the 5-FU sensitivity of CRC in vitro and in vivo by promoting apoptosis and autophagic cell death.Furthermore,autophagy played a dominant role in PCDH17-induced cell death,as an autophagy inhibitor blocked cell death to a greater extent than the pancaspase inhibitor Z-VAD-FMK.PCDH17 inhibition by siRNA decreased the autophagy response and 5-FU sensitivity.Mechanistically,we showed that c-Jun NH2-terminal kinase(JNK)activation was a key determinant in PCDH17-induced autophagy.The compound SP600125,an inhibitor of JNK,suppressed autophagy and 5-FU-induced cell death in PCDH17-reexpressing CRC cells.Taken together,our findings suggest for the first time that PCDH17 increases the sensitivity of CRC to 5-FU treatment by inducing apoptosis and JNK-dependent autophagic cell death.PCDH17 may be a potential prognostic marker for predicting 5-FU sensitivity in CRC patients. | Shuiping Liu Haoming Lin Da Wang Qiang Li Hong Luo Guoxiong Li Xiaohui Chen Yongqiang Li Peng Chen Bingtao Zhai Wengang Wang Ruonan Zhang Bi Chen Mingming Zhang Xuemeng Han Qiujie Li Liuxi Chen Ying Liu Xiaying Chen Guohua Li Yu Xiang Ting Duan Jiao Feng Jianshu Lou Xingxing Huang Qin Zhang Ting Pan Lili Yan Ting Jin Wenzheng Zhang Lvjia Zhuo Yitian Sun Tian Xie Xinbing Sui | 2019 | Signal Transduction and Targeted Therapy2019，4，1： | 4 |
| 2 | Fabrication of Ti/Al/Mg laminated composites by hot roll bonding and their microstructures and mechanical properties显示文摘Ti/Al/Mg laminated composites were successfully fabricated by hot roll bonding.The effects of the rolling reduction on the microstructural evolution and mechanical properties of the composites were explored.The results show that Ti/Al/Mg laminated sheets exhibit good interfacial bonding.The rolling reduction has a significant effect on the deformation inhomogeneity through the thickness of the Al layer.The initial grains of the Al layer near the Ti/Al interface are fragmented into fine equiaxed grains,and the grains at the center and near the Al/Mg interface are elongated.The R-cube shear texture of the Al layer forms near the Ti/Al interface and permeates into the center layer in the samples with greater rolling reductions.The b-fiber rolling texture of the Al layer is observed near the Al/Mg interface and increases with the increase of rolling reduction.The stress–strain curves indicate that the fracture appears first in the Mg layer.With the increasing rolling reduction,the ultimate tensile and yield strength values increase,and the elongation up to the Mg layer fracture decreases. | Pengju WANG Zejun CHEN Hongtao HUANG Jianshu LIN Boxin LI Qing LIU | 2021 | Chinese Journal of Aeronautics2021，34，8： | 4 |
| 3 | Simultaneous NF‐κB inhibition and E‐cadherin upregulation mediate mutually synergistic anticancer activity of celastrol and SAHA in vitro and in vivo显示文摘 | Lin Zheng Yingying Fu Linhan Zhuang Renhua Gai Jian Ma Jianshu Lou Hong Zhu Qiaojun He Bo Yang | 2014 | Int. J. Cancer2014，，： | 1 |
| 4 | Recursive identification of time-varying systems: Self-tuning and matrix RLS algorithms显示文摘 | Jianshu Li Yuanjin Zheng Zhiping Lin | 2014 | Systems & Control Letters2014，，： | 1 |
| 5 | A thermohydrodynamic analysis of dry gas seals for high-temperature gas-cooled reactor 显示文摘 | Wang Hong Zhu Baoshan Lin Jianshu | 2013 | Journal of Tribology2013，135，02： | 1 |
| 6 | Optimized collaboration of the first and third signals endows robust activity to T cells within the immunocompetent tumor microenvironment显示文摘CAR T-cell therapy has shown remarkable potential in the treatment of hematologic malignancies,and these successes have greatly motivated research on treatment of solid tumors.Although numerous clinical trials have been actively carried out,exciting clinical responses are still sporadic and may be are transient for patients with solid tumors[1]. | Jianshu Wei Xin Lin Weidong Han | 2023 | Cellular & Molecular Immunology2023，20，11： | 0 |
| 7 | pPeOp inhibits HGC-27 cell proliferation,migration and invasion by upregulating miR-30b-5p and down-regulating the Rac1/Cdc42 pathway显示文摘Gastric cancer is the fifth most frequently occurring and the fourth most lethal malignant cancer worldwide.A bioactive protein(pPeOp)from Omphalia lapidescens exhibits significant inhibitory effects on gastric cancer cells.miRNA deep sequencing analysis shows that miR-30b-5p is significantly upregulated in HGC-27 cells treated with pPeOp.Verification results show that the expression level of miR-30b-5p is significantly increased in HGC-27 cells after pPeOp treatment.Additionally,miR-30b-5p is significantly downregulated in clinical gastric cancer tissues compared to that in adjacent normal tissues.Following pPeOp treatment and/or transfection with miR-30b-5p mimic,the proliferation,migration,and invasion of HGC-27 cells are significantly impaired.Immunofluorescence microscopy shows that pPeOp and/or miR-30b-5p destroy(s)microfilaments and microstructures and inhibit(s)the formation of pseudopodia.Bioinformatics analysis,dual-luciferase reporter assay,and western blot analysis confirm that miR-30b-5p downregulates Rac1/Cdc42 expression and activation by targeting RAB22A.Available data indicate that miR-30b-5p plays an anti-gastric cancer role in mediating pPeOp.pPeOp upregulates miR-30b-5p expression,which in turn inhibits RAB22A expression,resulting in a reduction in the expression and activation of Rac1 and Cdc42 and their downstream targets,thus destroying the cytoskeletal structure and inhibiting the proliferation,migration,and invasion of cancer cells. | Wenjun Xu Zhenjie Fu Yuqin Xu Man Hei Cheung Yan Chen Meiai Lin Hang Wen Hang Lv Chun Liang Jianshu Lou Yitao Chen | 2022 | Acta Biochimica et Biophysica Sinica2022，54，12： | 0 |
| 8 | Supramolecular assembly of Cp1-11 peptide and insulin for rapid-acting formulation显示文摘In order to improve the life quality of diabetic patients,it is very important to develop rapid-acting insulin formulations that can mimic the physiological meal-time secretion profile of insulin in healthy people.Although several insulin analogues have been designed to provide postprandial glycemic control,still there are some serious disadvantages.A supramolecular strategy is presented here to inhibit insulin aggregation and improve its bioactivity by using Cp1-11 peptide.As a fragment of C-peptide in proinsulin,Cp1-11 peptide was found to influence insulin oligomerization by supramolecular interactions.This work demonstrates that the Cp1-11 peptide can interact with oligomeric insulin and facilitate its disaggregation into the physiologically active monomeric form.Computer simulation indicates that Cp1-11 can insert into the space between the C-terminal tail and the N-terminal helix of the B-chain of insulin,causing dissociation of the insulin dimer.The supramolecular assembly of Cp1-11 and insulin can improve the bioavailability and therapeutic effect of insulin on the control of in vivo blood glucose levels.These results suggest that Cp1-11 peptide can modulate the intermolecular interaction of aggregated insulin and prevent the transition from monomeric to multimeric states,and shows great potential for the development of an effective rapid-acting strategy to treat diabetes. | Weigang Wang Sheyu Li Zhouxiang Zhao Anna Zhou Yanpeng Liu Yantao Chen Mingchang Lin Guosong Chen Chunmei Ding Jianshu Li | 2017 | Journal of Bioresources and Bioproducts2017，2，3： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员