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| 1 | Study of the mechanisms of acupuncture and moxibustion treatmentfor ulcerative colitis ratsinview of the gene expression of cytokines显示文摘AIM To observe the effect of acupuncture and moxibustion on the expression of IL 1β and IL 6 mRNA in ulcerative colitis rats. METHODS The SD rat ulcerative colitis model was created by immunological method associated with local stimulation. Colonic mucosa was prepared from human fresh surgical colonic specimens, homogenized by adding appropriate amount of normal saline and centrifuged at 3000*!r/*!min . The supernatant was collected for measurement of protein conentration and then mixed with Freund adjuvant. This antigen fluid was first injected into the plantae of the model group rats, and then into their plantae, dorsa, inguina and abdominal cavities (no Freund adjuvant for the last injection) again on the 10th, 17th, 24th and 31st day. When a certain titer of serum anti colonic antibody was reached, 2% formalin and antigen fluid (no Freund adjuvant) were administered separately by enema. The ulcerative colitis rat model was thus set up. The animals were randomly divided into four groups: model control group (MC, n =8), electro acupuncture group (EA, n =8), herbs partition moxibustion group (HPM 8), normal control group (NC, n =8). HPM: Moxa cones made of refined mugwort floss were placed on the medicinal pad (medicinal pad dispensing: Radix Aconiti praeparata, cortex Cinnamomi, etc ) for Qihai (RN 6) and Tianshu (ST 25, bilateral) and ignited. Two moxa cones were used for each acupoint once a day and 14 times in all. EA: Tianshu (bilateral) and Qihai were stimulated by the intermittent pulse with 2Hz frequency, 4mA intensity for 20 minutes once a day and 14 times in all. After treatment, rats of all four groups were killed simultaneously. The spleen was separated and the distal colon was dissected. Total tissue RNA was isolated by the guanidinium thiocyanate phenol chloroform extraction method. RT PCR technique was used to study the expression of IL 1β and IL 6 mRNA. RESULTS IL 1β and IL 6 mRNAs were not detected in the spleen and colonic mucosa of the NC rats, whereas they were significantly expressed in that of the MC rats. IL 1β and IL 6 mRNAs were markedly lower in the EA and HPM rats than that in MC rats. There was no significant difference between the levels of IL 1β and IL 6 mRNAs in the EA and HPM rats. The expressions of IL 1β and IL 6 mRNAs were nearly the same in the spleen and colon of all groups. CONCLUSION Acupuncture and moxibustion greatly inhibited the expression of IL 1β and IL 6 mRNA in the experimental ulcerative colitis rats. | Wu HG Zhou LB Pan YY Huang C Chen HP Shi Z Hua XG | 1999 | World Journal of Gastroenterology1999，5，6： | 45 |
| 2 | miR-182参与调节胶质母细胞瘤的凋亡、生长和分化(英文)显示文摘Glioblastoma multiforme(GBM)is a lethal,therapy-resistant brain cancer consisting of numerous tumor cell subpopulations,including stem-like glioma-initiating cells(GICs),which contribute to tumor recurrence following initial response to therapy.Here,we identified miR-182 as a regulator of apoptosis,growth,and differentiation programs whose expression level is correlated with GBM patient survival.Repression of Bcl2-like12(Bcl2L12),c-Met,and hypoxia-inducible factor 2α(HIF2A)is of central importance to miR-182 anti-tumor activity,as it results in enhanced therapy susceptibility,decreased GIC sphere size,expansion,and stemness in vitro.To evaluate the tumor-suppressive function of miR-182 in vivo,we synthesized miR-182-based spherical nucleic acids(182-SNAs);i.e.,gold nanoparticles covalently functionalized with mature miR-182 duplexes.Intravenously administered 182-SNAs penetrated the bloodbrain/blood-tumor barriers(BBB/BTB)in orthotopic GBM xenografts and selectively disseminated throughout extravascular glioma parenchyma,causing reduced tumor burden and increased animal survival.Our results indicate that harnessing the anti-tumor activities of miR-182 via safe and robust delivery of 182-SNAs represents a novel strategy for therapeutic intervention in GBM. | Kouri FM Hurley LA Daniel WL Day ES Hua Y Hao L Peng CY Merkel TJ Queisser MA Ritner C Zhang H James CD Sznajder JI Chin L Giljohann DA Kessler JA Peter ME Mirkin CA Stegh AH | 2015 | 中华神经外科疾病研究杂志2015，14，2： | 14 |
| 3 | Tracing method study of bacterial translocation in vivo显示文摘INTRODUCTIONEndogenesis infection plays an important role innosocomial infection.By studying progress ofbacteria translocation from intestinal tract,theconcept of gut-origin infection has been acceptedgradually.Because of no ideal tracing method,there were some controversies.In order to solve theproblem,the PUC19 plasmid vector tracing | Fu WL Xiao GX Yue XL Hua C Lei MP | 2000 | World Journal of Gastroenterology2000，6，1： | 9 |
| 4 | Lipid-dependent conformational dynamics underlie the functional versatility of T-cell receptor显示文摘T 房间 receptor-CD3 建筑群(TCR ) 是能基于 CD3 细胞质的领域的各种各样的生物化学的变化开始抗原特定的有免疫力的回答的一台万用的发信号机器,但是内在的结构的基础留下逃犯。这里,我们开发了生物物理的途径学习 CD3 细胞质的领域的 conformational 动力学(CD3 CD ) 。在单个分子的水平,我们发现那 CD3 CD 能与三个功能的主题的不同坦诚有多重 conformational 状态,即, ITAM, BRS 和 PRS。因为,这些符合构造被产生 CD3 CD 有异构的类脂化合物绑定性质因此有异构的动力学。实时房间的成像实验证明不同抗原刺激能稳定 CD3 在不同符合构造的 CD 。类脂化合物依赖者 conformational 动力学因此为 TCR 的万用的发信号性质提供结构的基础。 | Xingdong Guo Chengsong Yan Hua Li Wenmao Huan Xiaoshan Shi Min Huang Yingfang Wang Weiling Pan Mingjun Cai Lunyi Li Wei Wu Yibing Bai Chi Zhang Zhijun Liu Xinyan Wang Xiaohui F Zhang Chun Tang Hongda Wang Wanli Liu Bo Ouyang Catherine C Wong Yi Cao Chenqi Xu | 2017 | Cell Research2017，27，4： | 5 |
| 5 | On the solitary wave solutions of the CQNLS显示文摘 | Hua C C Li K T | 2005 | Chaos Solitons and Fractals2005，25，5： | 1 |
| 6 | Design of high efficiency, low profile, low voltage converter with integrated magnetics显示文摘 | Hua G Sable D Lee F C | 1997 | IEEE APEC1997，，： | 1 |
| 7 | Heteronuclear 2D NMR studies of an engineered insulin monomer:assignment and characterization of the receptor-bingding surface by selective 2H and 13C labeling with application to protein design显示文摘 | Hua Q X Lynch C S | 1991 | Biochemistry1991，30，： | 1 |
| 8 | Compact multiband planar monopole antennas for smart application 显示文摘 | R C Hua C F Chou | 2008 | IET Microw Antennas Propag2008，2，5： | 1 |
| 9 | Effect of valsartan and captopril in rabbit carotid injury:possible involvement of bradykinin in the anti-proliferative action of the reninangiotensin blockade显示文摘 | Tong C F Ying W Y Hua R Y | 2001 | JRAAS2001，2，1： | 1 |
| 10 | (A) 显示文摘 | Cao Huai xin Xu zong ben Zhang Jian hua Matrix-trace on C^*- algebra M | 2002 | Linear Algebra and its Applications2002，，345： | 1 |
| 11 | Dexmedetomidine and ST91 analgesia in the formalin model is mediated by alpha2Aadrenoceptors:a mechanism of action distinct from morphine显示文摘 | NAZARIAN A CHRISTIANSON C A HUA X Y | 2008 | Br J Pharmacol2008，155，7： | 1 |
| 12 | High levels of mitochon- drial heteroplasmy modify the development of ovine-bovine in- terspecies nuclear transferred embryos 显示文摘 | HUA S LU C L SONG Y K | 2012 | Reproduction Fer- tility and Development2012，24，： | 1 |
| 13 | Chelation-assisted method for the preparation of cathode material LiFePO4显示文摘 | LI C HUA N WANG C | | 0，，： | 1 |
| 14 | Separation of fenoxaprop-p-ethyl biodegradation products by HPTLC显示文摘 | Song L Y Zhao Y C Hua R M | 2005 | J Planar Chromatogr2005，18，： | 1 |
| 15 | Degrada- tion of chlorpyrifos alone and in combillation with chlorotha- lonil and their effects on soil microbial populations 显示文摘 | Xiaoqiang C H U Hua F Xuedong P A N | | Jour- nal of Environmental Sciences0，20，4： | 1 |
| 16 | Effects of soybean oligosac-charides on antioxidant enzyme activities and insulin resis-tance in pregnant women with gestational diabetes melli-tus 显示文摘 | Fei BB Ling L Hua C | 2014 | Food Chem2014，158，5： | 1 |
| 17 | Top-k typicality queries and efficient query answering methods on large databases显示文摘 | Hua M Pei J Fu A W C | 2009 | The International Journal on Very Large Data Bases(VLDB)2009，32，18： | 1 |
| 18 | Achievable rates of full-duplex MIMO radios in fast fading channels with imperfect channel estimation显示文摘 | CIRIK A C RONG Y HUA Y | 2014 | IEEE Transactions on Signal Processing2014，62，15： | 1 |
| 19 | Effects of NaCl stress on photochemical aclivity and thylakoid membrane polypeptide composition of a salt-tolerant and a salt sensitive rice cultivar显示文摘 | WANG R L HUA C ZHOU F | 2009 | Photosynthetica2009，47，1： | 1 |
| 20 | Soft-switching techniques in PWM converters显示文摘 | HUA Guichao LEE F C | 1995 | IEEE Transactions on Industrial Electronics1995，42，6： | 1 |
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