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| 1 | Identification of tumor associated single-chain Fv by panning and screening antibody phage library using tumor cells显示文摘AIM:To study the feasibility of panning and screeningphage-displaying recombinant single-chain variablefragment (ScFv) of anti-tumor monoclonal antibodiesfor fixed whole cells as the carriers of mAb-bindingantigens.ME,ODS: The recombinant phage displaying librariesfor anti-colorectal tumor mAb MC3Ab, MC5Ab andanti-gastric tumor mAb MGD1 was constructed.Panning and screening were carried outby means ofmodified fixation of colorectal and gastric tumor cellsexpressed the mAb-binding antigens. Concordance ofbinding specificity to tumor cells between phageclones and parent antibodies was analyzed. Thephage of positive clones was identified withcompetitive ELISA, and infected by E.coli HB2151 toexpress soluble ScFv.RESULTS:The ratio of positive clones to MC3-ScF-MC5-ScFv and MGD1-ScFv were 60 %, 24 % and 30 %. MC3-ScFv had Mr 32 000 confirmed by Western blot. Thespecificity to antigen had no difference between 4positive recombinant phage antibodies and MC3Ab.CONCLUSION:The modified process of fixing wholetumor cells is efficient, convenient and feasible to panand screen the phage-displaying ScFv of anti-tumormonoclonal antibodies. | Nie, YZ He, FT Li, ZK Wu, KC Cao, YX Chen, BJ Fan, DM | 2002 | World Journal of Gastroenterology2002，8，4： | 12 |
| 2 | NEW ALDALOIDS TONGOLINE AND TONGOLININE FROMDELPHINIUM TONGOLENSE显示文摘NEWALDALOIDSTONGOLINEANDTONGOLININEFROMDELPHINIUMTONGOLENSELanHE;YaoZuCHEN(TheChemistryDepartmentofLanzhouUniversity,Lanzhou7... | He, L Chen, YZ Ding, LS Li, BG | 1996 | Chinese Chemical Letters1996，7，6： | 4 |
| 3 | Hyperactivation of working memory-related brain circuits in newly diagnosed middle-aged type 2 diabetics显示文摘 | He XS Wang ZX Zhu YZ | 2015 | Acta Diabetol2015，52，1： | 1 |
| 4 | Effects of testosterone on cytokines and left ventricular remodeling following heart failure显示文摘 | Zhang YZ Xing XW He B | 2007 | Cell Physiol Biochem2007，20，6： | 1 |
| 5 | Effect of dietary- rare earth elements on growth performance and blood parameters of rats 显示文摘 | He ML Wang YZ Xu ZR | 2003 | Journal of Animal Physiology and Animal Nutrition2003，87，56： | 1 |
| 6 | Effects of testosterone on cytokines and left ventricular remodeling following heart failure 显示文摘 | Zhang YZ Xing XW He B | 2007 | Cell Physiol Biochem2007，20，6： | 1 |
| 7 | Hemorrhagic fever caused by a novel Bunyavirus in China: pathogenesis and correlates of fatal outcome 显示文摘 | Zhang YZ He YW Dai YA | 2012 | Clin Infect Dis2012，54，4： | 1 |
| 8 | Plasma fatty acid metabolic profiling and biomarkers of type 2 diabetes mellitus based on GC/MS and PLS-LDA 显示文摘 | Yi LZ He J Liang YZ | 2012 | FEBS Letters2012，580，30： | 1 |
| 9 | Effects of testosterone on cytokines and left ventricular remodeling following heart failure显示文摘 | Zhang YZ Xing XW He B | 2007 | Cell Physiol Biochem2007，，6： | 1 |
| 10 | Study of RNA interference targeting NET-1 combination with sorafenib for hepatocellular carcinoma therapy in vitro and in vivo显示文摘 | He S Wei YZ Wang GL | 2013 | Gastroenterol Res Pract2013，2013，68： | 1 |
| 11 | Study on the prognosis of patients with acute paraquat intoxication 显示文摘 | Liu P He YZ Wang HC | 2011 | Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi2011，29，3： | 1 |
| 12 | Plasma fatty acid metabolicprofiling and biomarkers of type 2 diabetes mellitus based on GC! MS and PLS-LDA 显示文摘 | Yi LZ He J Liang YZ | 2006 | FEBS Letters2006，580，： | 1 |
| 13 | Association between RAD51 gene polymorphism(-135G/C)and susceptibility of myelodysplastic syndrome and acute leukemia:evidence based on a meta-analysis显示文摘 | He YZ Hu X Chi XS | 2014 | Tumour Biol2014，35，： | 1 |
| 14 | Hemorrhagic fever caused by a novel bunyavirus in China:Pathogensis and correlates of fatal outcome显示文摘 | Zhang YZ He YW Dai YA | 2012 | Clin Infect Dis2012，54，4： | 1 |
| 15 | Plasma fatty acid metabolic profiling and biomarkers of type 2 diabetes mellitus based on GC/MS and PLS-LDA显示文摘 | YI LZ HE J LIANG YZ | 2006 | FEBS Lett2006，580，： | 1 |
| 16 | Effects of intratracheal administration of nuclear factor-kappaB decoy oligodeoxynucleotides on long-term cigarette smoke-induced lung inflammation and pathology in mice显示文摘 | Li YT He B Wang YZ | 2009 | Respir Res2009，25，10： | 1 |
| 17 | Characterization of a Hy-perthe-rmo- stable Fe-superoxide dismutase from hot spring 显示文摘 | He YZ Fan KQ Jia C J | 2007 | Appl Mierobiol Biotechno12007，75，2： | 1 |
| 18 | Draft genome of the kiwifruit Actinidia chinensis 显示文摘 | Huang SX Ding J Deng DJ Tang W Sun HH Liu DY Zhang L Niu XL Zhang X Meng M Yu JD Liu J Han Y Shi W Zhang DF Cao SQ Wei ZJ Cui YL Xia YH Zeng HP Bao K Lin L Min Y Zhang H Miao M Tang XF Zhu YZ Sui Y Li GW Sun HJ Yue JY Sun JQ Liu FF Zhou LQ Lei L Zheng XQ Liu M Huang L Song J Xu CH Li JW Ye KY Zhong SL Lu BR He GH Xiao FM Wang HL Zheng HK Fei ZJ Liu YS | 2013 | Nat Commun2013，4，： | 1 |
| 19 | Direct production of ethanol from raw sweet potato starch using genetically engineered Zymomonas mobilis 显示文摘 | He MX Feng H Bai F Li Y Liu X Zhang YZ | 2009 | Afr J Mierobiol Res2009，3，11： | 1 |
| 20 | Diversity of both the cultivable protease-producing bacteria and their extracellular proteases in the sediments of the South China Sea显示文摘 | Zhou MY Chen XL Zhao HL Dang HY Luan XW Zhang XY He HL Zhou BC and Zhang YZ | | 0，，03： | 1 |
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