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| 1 | Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system显示文摘允许的技术内长的基因的特定的规定为基因功能的学习是珍贵的并且在治疗学有大潜力。我们创造了在 CRISPR 上系统,由核酸酶死者 Cas9 (dCas9 ) 蛋白质组成的二部件的 transcriptional 使活跃之物与互补顺序与 transcriptional 激活域和单个指南 RNA (sgRNAs ) 熔化了到基因倡导者。我们证明在 CRISPR 上能高效地以一种悦耳的方式在人和老鼠房间激活外长的记者基因。另外,我们证明在 vivo 的柔韧的记者基因激活能被把系统部件注入老鼠接合子完成。而且,我们证明在 CRISPR 上能激活内长的 IL1RN, SOX2,和 OCT4 基因。最有效的基因激活被对近似倡导者有约束力的 3-4 sgRNAs 的簇完成,建议他们在基因正式就职的 synergistic 行动。显著地,当指向多重基因的 sgRNAs 同时被介绍进房间时,柔韧的多路的内长的基因激活被完成。染色体宽的表示介绍表明了系统的高特性。 | Albert W Cheng Haoyi Wang Hui Yang Linyu Shi Yarden Katz Thorold W Theunissen Sudharshan Rangarajan Chikdu S Shivalila Daniel B Dadon Rudolf Jaenisch | 2013 | Cell Research2013，23，10： | 65 |
| 2 | CRISPR-Cas9-mediated multiplex gene editing in CAR-T cells显示文摘 | Xiaojuan Liu Yongping Zhang Chen Cheng Albert W Cheng Xingying Zhang Na Li Changqing Xia Xiaofei Wei Xiang Liu Haoyi Wang | 2017 | Cell Research2017，27，1： | 40 |
| 3 | Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation and genomic labeling显示文摘 | Albert W Cheng Nathaniel Jillette Phoebe Lee Dylan Plaskon Yasuhiro Fuj iwara Wenbo Wang Aziz Taghbalout Haoyi Wang | 2016 | Cell Research2016，26，2： | 19 |
| 4 | Simulation of the piezoelectric high-speed on/off valve显示文摘The development trend and unsolved problems of the high-speed valve are given briefly, and the excellent characteristics and some key technologies of the piezoelectric high-speed on/off valve are introduced. An innovative high-speed on/off valve based on piezoelectric actuators is outlined, where the performance of the high frequency and high instant force is employed to kick off the poppet. The micro displacement amplification and temperature dependence of the piezoelectric actuator are solved. Furthermore, the mathematical model of the piezoelectric high-speed on/off valve is built and related simulation results are discussed. The simulation results show that the piezoelectric high-speed on/off valve can be switched by piezoelectric actuators, and the maximal flowrate of 11 L/min, pressure of 20 MPa and frequency of 200 Hz can be gained. | OUYANG XiaoPing YANG HuaYong JIANG HaoYi XU Bing | 2008 | Chinese Science Bulletin2008，53，17： | 11 |
| 5 | Fabrication of NiFe layered double hydroxide with well-defined laminar superstructure as highly efficient oxygen evolution electrocatalysts显示文摘Structure-activity relationship (SAR) is the key problem of nanoscience,thus to fabricate novel and well-defined nanostructure will provide a new insight on catalyst preparation method.Highly active and low cost electrocatalysts for oxygen evolution reaction (OER) are of great importance for future renewable energy conversion and storage.Herein,NiFe-based layered double hydroxides with laminar structure (NFLS) were successfully fabricated via a one-step hydrothermal approach by using sodium dodecyl sulfate as surfactant.The as-fabricated NFLS showed a well-defined periodic layered-stacking geometry with a scale down to 1-nm.Benefitting from the unique structure,NFLS exhibited an excellent catalytic activity towards OER with current densities of 10 mA·cm^-2 at overpotential of 197 mV.The synergistic effect of Ni and Fe plays a key role in electrode reactions.The present work provides a new insight to improve the OER performance by rational design of electrocatalysts with unique structures. | Hao Zhang Haoyi Li Bilal Akram Xun Wang | 2019 | Nano Research2019，12，6： | 10 |
| 6 | Whole-Genome Resequencing of a Worldwide Collection of Rapeseed Accessions Reveals the Genetic Basis of Ecotype Divergence显示文摘Rapeseed (Brassica napus),an important oilseed crop,has adapted to diverse climate zones and latitudes by forming three main ecotype groups,namely winter,semiwinter,and spring types. However,genetic variations underlying the divergence of these ecotypes are largely unknown. Here,we report the global pattern of genetic polymorphisms in rapeseed determined by resequencing a worldwide collection of 991 germplasm accessions.A total of 5.56 and 5.53 million singlenucleotide polymorphisms (SNPs)as Well as 1.86 and 1.92 million InDels were identified by mapping reads to the reference genomes of 'Darmor-bzh'and 'Tapidor,'respectively.We generated a map of allelic drift paths that shows splits and mixtures of the main populations,and revealed an asymmetric evolution of the two subgenomes of B.napus by calculating the genetic diversity and linkage disequilibrium parameters.Selective-sweep analysis revealed genetic changes in genes orthologous to those regulating various aspects of plant development and response to stresses.A genome-wide association study identified SNPs in the promoter regions of FLOWERING LOCUS T and FLOWERING LOCUS C orthologs that corresponded to the different rapeseed ecotype groups. Our study provides important insights into the genomic footprints of rapeseed evolution and flowering-time divergence among three ecotype groups,and will facilitate screening of molecular markers for accelerating rapeseed breeding. | Dezhi Wu Zhe Liang Tao Yan Ying Xu Lijie Xuan Juan Tang Gang Zhou Ulrike Lohwasser Shuijin Hua Haoyi Wang Xiaoyang Chen Qian Wang Le Zhu Antony Maodzeka Nazim Hussain Zhilan Li Xuming Li Imran Haider Shamsi Ghulam Jilani Linde Wu Hongkun Zheng Guoping Zhang Boulos Chalhoub Lisha Shen Hao Yu Lixi Jiang | 2019 | Molecular Plant2019，12，1： | 10 |
| 7 | CRISPR-Cas9 mediated LAG-3 disruption in CAR-T cells显示文摘 | Yongping Zhang Xingying Zhang Chen Cheng Wei Mu Xiaojuan Liu Na Li Xiaofei Wei Xiang Liu Changqing Xia Haoyi Wang | 2017 | Frontiers of Medicine2017，11，4： | 9 |
| 8 | Numerical analysis of broadband noise reduction with wavy leading edge显示文摘Large Eddy Simulation(LES) is performed to investigate the airfoil broadband noise reduction with wavy leading edge under anisotropic incoming turbulence. The anisotropic incoming turbulence is generated by a rod with a diameter of 10 mm. The incoming flow velocity is 40 m/s and the corresponding Reynolds numbers based on airfoil chord and rod diameter are about 397000 and 26000, respectively. The far-field acoustic field is predicted using an acoustic analogy method which has been validated by the experiment. A straight leading edge airfoil and a wavy leading edge airfoil are simulated. The results show that wavy leading edge increases the airfoil lift and drag whereas the lift and drag fluctuations are substantially reduced. In addition, wavy leading edge can significantly change the flow pattern around the leading edge and a pair of counter-rotating streamwise vortices stemming from each wavy leading edge peak are observed.An averaged noise reduction of 9.5 dB is observed with the wavy leading edge at the azimuthal angle of 90°. Moreover, the wavy leading edge can mitigate noise radiation at all the azimuthal angles without significantly changing the noise directivity. The underlying noise reduction mechanisms are then analyzed in detail. | Fan TONG Weiyang QIAO Weijie CHEN Haoyi CHENG Renke WEI Xunnian WANG | 2018 | Chinese Journal of Aeronautics2018，31，7： | 8 |
| 9 | Gene editing and its applications in biomedicine显示文摘The steady progress in genome editing, especially genome editing based on the use of clustered regularly interspaced short palindromic repeats(CRISPR) and programmable nucleases to make precise modifications to genetic material, has provided enormous opportunities to advance biomedical research and promote human health. The application of these technologies in basic biomedical research has yielded significant advances in identifying and studying key molecular targets relevant to human diseases and their treatment. The clinical translation of genome editing techniques offers unprecedented biomedical engineering capabilities in the diagnosis, prevention, and treatment of disease or disability. Here, we provide a general summary of emerging biomedical applications of genome editing, including open challenges. We also summarize the tools of genome editing and the insights derived from their applications, hoping to accelerate new discoveries and therapies in biomedicine. | Guanglei Li Xiangyang Li Songkuan Zhuang Liren Wang Yifan Zhu Yangcan Chen Wen Sun Zeguang Wu Zhuo Zhou Jia Chen Xingxu Huang Jin Wang Dali Li Wei Li Haoyi Wang Wensheng Wei | 2022 | Science China(Life Sciences)2022，65，4： | 8 |
| 10 | Phase I study of CAR-T cells with PD-1 and TCR disruption in mesothelin-positive solid tumors显示文摘Programmed cell death protein-1(PD-1)-mediated immunosuppression has been proposed to contribute to the limited clinical efficacy of chimeric antigen receptor T(CAR-T)cells in solid tumors.We generated PD-1 and T cell receptor(TCR)deficient mesothelin-specific CAR-T(MPTK-CAR-T)cells using CRISPR-Cas9 technology and evaluated them in a dose-escalation study.A total of 15 patients received one or more infusions of MPTK-CAR-T cells without prior lymphodepletion.No dose-limiting toxicity or unexpected adverse events were observed in any of the 15 patients.The best overall response was stable disease(2/15 patients).Circulating MPTK-CAR-T cells peaked at days 7–14 and became undetectable beyond 1 month.TCR-positive CAR-T cells rather than TCR-negative CAR-T cells were predominantly detected in effusion or peripheral blood from three patients after infusion.We further confirmed the reduced persistence of TCR-deficient CAR-T cells in animal models.Our results establish the preliminary feasibility and safety of CRISPR-engineered CAR-T cells with PD-1 disruption and suggest that the natural TCR plays an important role in the persistence of CAR-T cells when treating solid tumors. | Zhenguang Wang Na Li Kaichao Feng Meixia Chen Yan Zhang Yang Liu Qingming Yang Jing Nie Na Tang Xingying Zhang Chen Cheng Lianjun Shen Jiaping He Xun Ye Wei Cao Haoyi Wang Weidong Han | 2021 | Cellular & Molecular Immunology2021，18，9： | 7 |
| 11 | Artificial neural networks enabled by nanophotonics显示文摘The growing demands of brain science and artificial intelligence create an urgent need for the development of artificial neural networks(ANNs)that can mimic the structural,functional and biological features of human neural networks.Nanophotonics,which is the study of the behaviour of light and the light–matter interaction at the nanometre scale,has unveiled new phenomena and led to new applications beyond the diffraction limit of light.These emerging nanophotonic devices have enabled scientists to develop paradigm shifts of research into ANNs.In the present review,we summarise the recent progress in nanophotonics for emulating the structural,functional and biological features of ANNs,directly or indirectly. | Qiming Zhang Haoyi Yu Martina Barbiero Baokai Wang Min Gu | 2019 | Light(Science & Applications)2019，8，1： | 7 |
| 12 | One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering显示文摘 | Haoyi Wang Hui Yang Chikdu S. Shivalila Meelad M. Dawlaty Albert W. Cheng Feng Zhang Rudolf Jaenisch | 2013 | Cell2013，，4： | 5 |
| 13 | Multi-node CdS hetero-nanowires grown with defect-rich oxygen-doped MoS2 ultrathin nanosheets for efficient visible-light photocatalytic H2 evolution显示文摘Developing low-cost and high-efficiency photocatalysts for hydrogen production from solar water splitting is intriguing but challenging. In this study, unique one-dimensional (1D) multi-node MoS2/CdS hetero-nanowires (NWs) for efficient visible-light photocatalytic H2 evolution are synthesized via a facile hydrothermal method. Flower-like sheaths are assembled from numerous defect-rich O-incorporated {0001} MoS2 ultrathin nanosheets (NSs), and {112̅0}-facet surrounded CdS NW stems are grown preferentially along the c-axis. Interestingly, the defects in the MoS2 NSs provide additional active S atoms on the exposed edge sites, and the incorporation of O reduces the energy barrier for H2 evolution and increases the electric conductivity of the MoS2 NSs. Moreover, the recombination of photoinduced charge carriers is significantly inhibited by the heterojunction formed between the MoS2 NSs and CdS NWs. Therefore, in the absence of noble metals as co-catalysts, the 1D MoS2 NS/CdS NW hybrids exhibit an excellent H2-generation rate of 10.85 mmol·g-1·h-1 and a quantum yield of 22.0% at λ = 475 nm, which is far better than those of Pt/CdS NWs, pure MoS2 NSs, and CdS NWs as well as their physical mixtures. Our results contribute to the rational construction of highly reactive nanostructures for various catalytic applications. | Haifeng Lin Yanyan Li Haoyi Li Xun Wang | 2017 | Nano Research2017，10，4： | 5 |
| 14 | Questions about NgAgo显示文摘 | Shawn Burgess Linzhao Cheng Feng Gu Zhiwei Huang Shuo Lin Jinsong Li Wei Li Wei Qin Yujie Sun Zhou Songyang Wensheng Wei Qiang Wu Haoyi Wang Xiaoqun Wang Jing-Wei Xiong Jianzhong Xi Hui Yang Bin Zhou Bo Zhang Junjiu Huang | 2016 | Protein & Cell2016，7，12： | 5 |
| 15 | Delivery of Cas9 Protein into Mouse Zygotes through a Series of Electroporation Dramatically Increases the Efficiency of Model Creation显示文摘Previously we established Zygote Electroporation of Nucleases(ZEN) technology as an efficient and high-throughput way to generate genetically modified mouse models.However,there were significant variations of the targeting efficiency among different genomic loci using our previously published protocol.In this study,we improved the ZEN technology by delivering Cas9 protein into mouse zygotes through a series of electroporation.Using this approach,we were able to introduce precise nucleotide substitutions,large segment deletion and short segment insertion into targeted loci with high efficiency. | Wenbo Wang Peter M.Kutny Shannon L.Byers Charles J.Longstaff Michael J.DaCosta Changhong Pang Yingfan Zhang Robert A.Taft Frank W.Buaas Haoyi Wang | 2016 | Journal of Genetics and Genomics2016，43，5： | 4 |
| 16 | Glucocorticoid receptor modulates myeloid-derived suppressor cell function via mitochondrial metabolism in immune thrombocytopenia显示文摘Myeloid-derived suppressor cells(MDSCs)are a heterogeneous population of immature cells and natural inhibitors of adaptive immunity.Intracellular metabolic changes in MDSCs exert a direct immunological influence on their suppressive activity.Our previous study demonstrated that high-dose dexamethasone(HD-DXM)corrected the functional impairment of MDSCs in immune thrombocytopenia(ITP);however,the MDSC population was not restored in nonresponders,and the mechanism remained unclear.In this study,altered mitochondrial physiology and reduced mitochondrial gene transcription were detected in MDSCs from HD-DXM nonresponders,accompanied by decreased levels of carnitine palmitoyltransferase-1(CPT-1),a rate-limiting enzyme in fatty acid oxidation(FAO).Blockade of FAO with a CPT-1 inhibitor abolished the immunosuppressive function of MDSCs in HD-DXM responders.We also report that MDSCs from ITP patients had lower expression of the glucocorticoid receptor(GR),which can translocate into mitochondria to regulate the transcription of mitochondrial DNA(mtDNA)as well as the level of oxidative phosphorylation.It was confirmed that the expression of CPT-1 and mtDNA-encoded genes was downregulated in GR-siRNA-treated murine MDSCs.Finally,by establishing murine models of active and passive ITP via adoptive transfer of DXM-modulated MDSCs,we confirmed that GR-silenced MDSCs failed to alleviate thrombocytopenia in mice with ITP.In conclusion,our study indicated that impaired aerobic metabolism in MDSCs participates in the pathogenesis of glucocorticoid resistance in ITP and that intact control of MDSC metabolism by GR contributes to the homeostatic regulation of immunosuppressive cell function. | Yu Hou Jie Xie Shuwen Wang Daqi Li Lingjun Wang Haoyi Wang Xiaofei Ni Shaoqiu Leng Guosheng Li Ming Hou Jun Peng | 2022 | Cellular & Molecular Immunology2022，19，7： | 4 |
| 17 | Sediment microbial fuel cell with floating biocathode for organic removal and energy recovery显示文摘 | Aijie WANG Haoyi CHENG Nanqi REN Dan CUI Na LIN Weimin WU | 2012 | Frontiers of Environmental Science & Engineering2012，6，4： | 4 |
| 18 | Toward better electrode/electrolyte interfaces in the ionic-liquid-based rechargeable aluminum batteries显示文摘The past decade has witnessed the germination of rechargeable aluminum batteries(RABs)with the colossal potential to enact as a device for the large scale energy storage and conversion.The Majority of investigations are dedicated to the exploration of suitable cathode materials,while less is known about the electrode/electrolyte interfaces that determine the electrochemistry of batteries.In this perspective,we will highlight the significance of electrode/electrolyte interface for RABs,in overall kinetics and capacity retention.Emphasis will be laid on the complicated yet basic understandings of the phenomena at the interfaces,including the dendrite growth,surface Al2O3 and solid–electrolyte-interphase(SEI).And we will summarize the reported practice in effort to build better electrode/electrolyte interfaces in RAB.In the end,outlook regarding to the challenges,opportunities and directions is presented. | Haoyi Yang Feng Wu Ying Bai Chuan Wu | 2020 | Journal of Energy Chemistry2020，29，6： | 4 |
| 19 | Gene editing in T cell therapy显示文摘The adoptive transfer of engineered T cells for the treatment of cancer, autoimmunity, and infectious disease is a rapidly growing field that has shown great promise. Gene editing holds tremendous potential for further improvements of T cell therapy. Here we review the applications of gene editing in various T cell therapies, focusing on antiviral strategies and cancer immunotherapies, and discuss the challenges and future prospects. | Yongping Zhang Wei Mu Haoyi Wang | 2017 | Journal of Genetics and Genomics2017，44，9： | 3 |
| 20 | Insight to defects regulation on sugarcane waste-derived hard carbon anode for sodium-ion batteries显示文摘A great deal of attention has been paid on developing plant-derived hard carbon(HC)materials as anodes for sodium-ion batteries(SIBs).So far,the regulation of HC has been handicapped by the well-known ambiguity of Na^(+)storage mechanism,which fails to differentiate the Na^(+)adsorption and Na^(+)insertion,and their relationship with the size of d-interlayer spacing and structural porosity.Herein,bagassederived HC materials have been synthesized through a combination of pyrolysis treatment and microwave activation.The combined protocol has enabled to synergistically control the d-interlayer spacing and porosity.Specifically,the microwave activation has created slit pores into HC and these pores allow for an enhanced Na^(+)adsorption with an increased sloping capacity,establishing a strong correlation between the porosity and sloping capacity.Meanwhile,the pyrolysis treatment promotes the graphitization and it contributes to an intensified Na^(+)insertion with an increased plateau capacity,proving that the plateau capacity is largely contributed by the Na^(+)insertion between interlayers.Therefore,the structural regulation of bagasse-derived HC has provided a proof on positively explaining the Na^(+)storage with HC materials.The structural changes in the pore size distribution,specific surface area,d-interlayer spacing,and the electrochemical properties have been comprehensively characterized,all supporting our understanding of Na^(+)storage mechanism.As a result,the HC sample with an optimized d-interlayer spacing and porosity has delivered an improved reversible capacity of 323.6 m Ah g^(-1) at 50 m A g^(-1).This work provides an understanding of Na^(+)storage mechanism and insights on enhancing the sloping/plateau capacity by rationally regulating the graphitization and porosity of HC materials for advanced SIBs. | Kaihua Yu Xinran Wang Haoyi Yang Ying Bai Chuan Wu | 2021 | Journal of Energy Chemistry2021，30，4： | 3 |
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