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| 1 | Clay nanosheet-mediated delivery of recombinant plasmids expressing artificial miRNAs via leaf spray to prevent infection by plant DNA viruses显示文摘Whitefly-transmitted begomoviruses are economically important plant pathogens that cause severe problems in many crop plants,such as tomato,papaya,cotton,and tobacco.Tomato yellow leaf curl virus(TYLCV)is a typical monopartite begomovirus that has been extensively studied,but methods that can efficiently control begomoviruses are still scarce.In this study,we combined artificial microRNA(amiRNA)-mediated silencing technology and clay nanosheetmediated delivery by spraying and developed a method for efficiently preventing TYLCV infection in tomato plants.We designed three amiRNAs that target different regions of TYLCV to silence virus-produced transcripts.Three plant expression vectors expressing pre-amiRNAs were constructed,and recombinant plasmid DNAs(pDNAs)were loaded onto nontoxic and degradable layered double hydroxide(LDH)clay nanosheets.LDH nanosheets containing multiple pDNAs were sprayed onto plant leaves.We found that the designed amiRNAs were significantly accumulated in leaves 7 days after spraying,while the pDNAs were sustainably detected for 35 days after the spray,suggesting that the LDH nanosheets released pDNAs in a sustained manner,protected pDNAs from degradation and efficiently delivered pDNAs into plant cells.Importantly,when the LDH nanosheets coated with pDNAs were sprayed onto plants infected by TYLCV,both the disease severity and TYLCV viral concentration in sprayed plants were significantly decreased during the 35 days,while the levels of H_(2)O_(2) were significantly increased in those plants.Taken together,these results indicate that LDH nanosheets loaded with pDNAs expressing amiRNAs can be a sustainable and promising tool for begomovirus control. | Qili Liu Yanpeng Li Kedong Xu Dongxiao Li Haiyan Hu Feng Zhou Puwen Song Yongang Yu Qichao Wei Qian Liu Weipeng Wang Ruifang Bu Haili Sun Xiaohui Wang Jianjun Hao Honglian Li Chengwei Li | 2020 | Horticulture Research2020，7，1： | 1 |
| 2 | Seed germinating characteristics of 54 gramineous species in the alpine meadow on the eastern Qinghai-Tibet plateau显示文摘The germination experiment was started on March 3,2004,and seeds were collected from July to October in 2003.We analyzed the percentage of germination,days to first germination,germination period and days to 50%germination.Among the 54 examined species,26 species exceeded 80%germination,11 species exceeded 60%–80%germination,8 exceeded 40%–0%,5 exceeded 20%–40%,and 4 showed less than 20%.A principalcomponent analysis revealed that the species were distributed along two statistically independent axes,the first primarily represented the germination rate and the second represented the time of germination process.Based on scores of germination characteristics,cluster analysis of the 54 gramineous species could be divided into 4 distinct groups:rapid,slow,intermediate germinating(germination percentage>50%),and low germinating(germination percentage<50%).The meaning of different groups to the vegetation regeneration was discussed. | Haiyan BU Qingji REN Xiuli XU Kun LIU Peng JIA Shujun WEN Dashuai SUN Guozhen DU | 2008 | Frontiers in Biology2008，3，2： | 1 |
| 3 | Seed mass and germination in an alpine meadow on the eastern Tsinghai - Tibet plateau 显示文摘 | Haiyan Bu Xuelin Chen Xiuli Xu | 2007 | Plant Ecology2007，191，： | 1 |
| 4 | 放牧对青藏高原高寒草地种子萌发性状选择的影响显示文摘以前的研究表明放牧能够引起草地生物与非生物环境的显著变化,但这种变化影响草地群落对种子萌发特征的选择机制尚不清楚。因此,我们旨在回答:放牧是否对草地群落中萌发特征的组成和多样性产生显著影响。我们在实验室检测了研究草地群落内主要物种种子的萌发特性,并比较了这些植物在放牧和非放牧草地上的表现。在此基础上,比较了放牧草地和非放牧草地的各萌发性状的群落加权平均值和萌发性状多样性,从而了解放牧草地和非放牧草地是否存在不同的萌发性状结构。研究结果表明,在物种水平上,放牧和非放牧草地各物种的多度变化与物种的萌发性状无显著关系。但在群落水平上,与非放牧草地相比,放牧草地的物种普遍具有较高的种子萌发率;放牧草地种子萌发对变温的正响应显著大于非放牧草地,而且放牧草地种子萌发温度生态位宽度小于非放牧草地。与非放牧草地相比,放牧草地种子萌发性状多样性增加,萌发性状均匀度降低。放牧可以改变微生境,从而通过环境过滤改变草地群落对萌发性状的选择,导致草地群落的萌发性状于放牧前后在群落水平上存 在显著差异。 | Yang Liu Kun Liu Zhilong Zhang Shiting Zhang Carol C.Baskin Jerry M.Baskin Ting Liang Haiyan Bu Shuxia Li Tingting Zhang Xianliang Cui Sa Xiao | 2022 | Journal of Plant Ecology2022，15，4： | 0 |
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