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| 1 | Engineering Herbicide-Resistant Rice Plants through CRISPR/Cas9-Mediated Homologous Recombination of Acetolactate Synthase显示文摘 | Sun, Yongwei Zhang, Xin Wu, Chuanyin He, Yubing Ma, Youzhi Hou, Han Guo, Xiuping Du, Wenming Zhao, Yunde Xia, Lanqin | 2016 | Molecular Plant2016，9，4： | 94 |
| 2 | Programmed Self-Elimination of the CRISPR/Cas9 Construct Greatly Accelerates the Isolation of Edited and Transgene-Free Rice Plants显示文摘 | Yubing He Min Zhu Lihao Wang Junhua Wu Qiaoyan Wang Rongchen Wang Yunde Zhao | 2018 | Molecular Plant2018，11，9： | 18 |
| 3 | Self-cleaving ribozymes enable the production of guide RNAs from unlimited choices of promoters for CRISPR/Cas9 mediated genome editing显示文摘Development of tools for targeted modifications of specific DNA sequences in plants is of great importance to basic plant biology research as well as crop improvement.The ability to cut DNA at specific locations in the genome to generate doublestrand breaks(DSBs)in vivo is a prerequisite for any genome editing efforts.Several engineered nucleases including zinc finger nucleases(ZFNs)and transcriptional activator-like nucleases(TALENs)have been successfully used to edit genomes.However, | Yubing He Tao Zhang Ning Yang Meilian Xu Lang Yan Lihao Wang Rongchen Wang Yunde Zhao | 2017 | Journal of Genetics and Genomics2017，44，9： | 14 |
| 4 | Clinical phenotype network: the underlying mechanism for perso- nalized diagnosis and treatment of traditional Chinese medicine显示文摘繁体中文药(TCM ) 在个性化的药的一个典型纲要调查临床的诊断和治疗整齐,它意味着有一样的疾病的个性化的病人将从不同 TCM 医生获得不同诊断和最佳的治疗。这个原则被 TCM 临床的专业人员认出并且粘住几千年了。然而,个性化的药不是的 TCM 的内在的机制到目前为止充分调查了并且仍然保持未知。这份报纸讨论 TCM 的框架在经典文学并且在真实世界的临床的背景的个性化的药,并且调查 TCM 的内在的机制从网络药的观点的个性化的药。在失眠上基于 246 个设计得好的门诊病人记录,由评估表明观察的个人偏爱和植物药方的偏爱,我们注意了每张植物药方的重要类似和每相遇的症状类似。为了调查 TCM 的内在的机制,个性化药,我们构造了一个临床的显型网络(CPN ) ,在哪个临床的显型实体喜欢症状并且诊断作为连接表现为在这些实体之间的节点和关联。这 CPN 被用来调查症候群和症状的同现的混杂的边界。小世界的拓扑的特征与高聚类结构在 CPN 被注意,它在 TCM 的合理性上提供卓见个性化的诊断和处理。这个网络上的调查将帮助我们在 TCM 的内在的机制上获得理解个性化的药并且将为 TCM 的精炼建议一个新观点个性化的临床的技巧。 | Xuezhong Zhou Yubing Li Yonghong Peng Jingqing HU Runshun Zhang Liyun He Yinghui Wang Lijie Jiang Shiyan Yan Peng Li Qi Xie Baoyan Liu | 2014 | Frontiers of Medicine2014，8，3： | 7 |
| 5 | Technological breakthroughs in generating transgene-free and genetically stable CRISPR-edited plants显示文摘CRISPR/Cas9 gene-editing technologies have been very effective in editing target genes in all major crop plants and offer unprecedented potentials in crop improvement.A major challenge in using CRISPR gene-editing technology for agricultural applications is that the target gene-edited crop plants need to be transgene free to maintain trait stability and to gain regulatory approval for commercial production.In this article,we present various strategies for generating transgene-free and target geneedited crop plants.The CRISPR transgenes can be removed by genetic segregation if the crop plants are reproduced sexually.Marker-assisted tracking and eliminating transgenes greatly decrease the time and labor needed for identifying the ideal transgene-free plants.Transgenes can be programed to undergo self-elimination when CRISPR genes and suicide genes are sequentially activated,greatly accelerating the isolation of transgene-free and target gene-edited plants.Transgene-free plants can also be generated using approaches that are considered non-transgenic such as ribonucleoprotein transfection,transient expression of transgenes without DNA integration,and nano-biotechnology.Here,we discuss the advantages and disadvantages of the various strategies in generating transgene-free plants and provide guidance for adopting the best strategies in editing a crop plant. | Yubing He Yunde Zhao | 2020 | aBIOTECH2020，1，1： | 5 |
| 6 | Multistage analysis method for detection of effective herb prescription from clinical data显示文摘为特定的疾病条件或特别的耐心的组决定有效繁体中文药(TCM ) 治疗是因为复杂个性化的表明,在真实世界的病人和在临床的背景规定的个性化的联合治疗要求调查的一个困难的问题。在这研究,集成倾向大小写匹配的一个多级式的分析方法,复杂网络分析,和植物给了丰富分析被建议为特别疾病识别有效植物药方(例如,失眠) 。首先,匹配的倾向大小写被使用匹配临床的盒子。然后,核心网络抽取和植物集合丰富被联合检测核心有效植物药方。基于有效性的相互的信息被用来检测强壮的 herbsymptom 关系。这个方法在一个 TCM 临床的数据集合上被使用, 955 个病人从设计得好的观察研究收集了。结果与更高的有效性率揭示了那组植物药方(76.9% 对 42.8% 为匹配的样品;94.2% 对 84.9% 为所有样品) 与原版相比,药方被发现。有症状表明的特别的耐心的组也被识别帮助调查有效植物药方的指示。 | Kuo Yang Runshun Zhang Liyun He Yubing Li Wenwen Liu Changhe Yu Yanhong Zhang Xinlong Li Yan Liu Weiming Xu Xuezhong Zhou Baoyan Liu | 2018 | Frontiers of Medicine2018，12，2： | 5 |
| 7 | A reporter for noninvasively monitoring gene expression and plant transformation显示文摘Reporters have been widely used to visualize gene expression,protein localization,and other cellular activities,but the commonly used reporters require special equipment,expensive chemicals,or invasive treatments.Here,we construct a new reporter RUBY that converts tyrosine to vividly red betalain,which is clearly visible to naked eyes without the need of using special equipment or chemical treatments.We show that RUBY can be used to noninvasively monitor gene expression in plants.Furthermore,we show that RUBY is an effective selection marker for transformation events in both rice and Arabidopsis.The new reporter will be especially useful for monitoring cellular activities in large crop plants such as a fruit tree under field conditions and for observing transformation and gene expression in tissue culture under sterile conditions. | Yubing He Tao Zhang Hui Sun Huadong Zhan Yunde Zhao | 2020 | Horticulture Research2020，7，1： | 4 |
| 8 | Plant base editing and prime editing:The current status and future perspectives显示文摘Precise replacement of an allele with an elite allele controlling an important agronomic trait in a predefined manner by gene editing technologies is highly desirable in crop improvement.Base editing and prime editing are two newly developed precision gene editing systems which can introduce the substitution of a single base and install the desired short indels to the target loci in the absence of double-strand breaks and donor repair templates,respectively.Since their discoveries,various strategies have been attempted to optimize both base editor(BE)and prime editor(PE)in order to improve the precise editing efficacy,specificity,and expand the targeting scopes.Here,we summarize the latest development of various BEs and PEs,as well as their applications in plants.Based on these progresses,we recommend the appropriate BEs and PEs for both basic plant research and crop improvement.Moreover,we propose the perspectives for further optimization of these two editors.We envision that both BEs and PEs will become the routine and customized precise gene editing tools for both plant biological research and crop improvement in the near future. | Jingying Li Chen Zhang Yubing He Shaoya Li Lei Yan Yucai Li Ziwei Zhu Lanqin Xia | 2023 | Journal of Integrative Plant Biology2023，65，2： | 1 |
| 9 | A comparative study on the air-side performance of wavy fin-and-tube heat exchanger with punched delta winglets in staggered and in-line arrangements显示文摘 | Tian Liting He Yaling Tao Yubing | 2009 | International Journal of Thermal Science2009，48，9： | 1 |
| 10 | A key link between jasmonic acid signaling and auxin biosynthesis显示文摘A research team led by Xiang Cheng Bin from School of Life Sciences,University of Science and Technology of China discovered a molecular basis for crosstalk between jasmonic acid(JA)and auxin,two important plant hormones.Their findings were published in a recent issue of Nature Communication[1].Auxin is the plant growth hormone that plays an | HE YuBing ZHAO Yunde | 2015 | Science China(Life Sciences)2015，58，3： | 1 |
| 11 | Numerical study on coupled fluid flow and heat transfer process in parabolic trough solar collector tube 显示文摘 | Tao Yubing He Yaling | 2010 | Solar Energy2010，84，10： | 1 |
| 12 | Numerical study on performance of molten salt phase change thermal energy storage system with enhanced tubes 显示文摘 | Tao Yubing He Yaling Qu Zhiguo | 2012 | Solar Energy2012，86，5： | 1 |
| 13 | Numerical study on thermal energy storage performance of phase change material under non-steady-state inlet boundary 显示文摘 | Tao Yubing He Yaling | 2011 | Applied Energy2011，88，11： | 1 |
| 14 | Preparation and antioxidant activity of albumin binding Salen Schiff-base metal complexes显示文摘A kind of novel biopolymer antioxidant (BSA/HOSalenM, M=Co, Mn, Zn) is prepared with conjugation, which increases the antioxidant activity of the bovine serum albumin (BSA). The conjugations have been characterized by IR spectra, UV-Vis spectra, Fluorescence spectra, Circular dichroism (CD) spectra and Native-PAGE. The BSA is used as a biopolymer scaffold, and the insoluble Salen Schiff-base metal complexes HOSalenM make axial coordination with the amino acid residues of the BSA. The structure of the BSA is unchanged when the binding rate of HOSalenCo is less than 10. The HOSalenCo conjugations show an excellent hydroxyl radical (·OH) scavenging activity, and the activity (EC50 ) of BSA/HOSalenCo(10) (BSA : HOSalenCo=1 : 10) is improved by two orders of magnitude compared with the BSA, while the activity of the BSA/HOSalenMn is weak and the BSA/HOSalenZn shows no scavenging activity. | LI Gang ZHANG HuiFang WANG RongMin HE YuFeng XIONG YuBing | 2013 | Chinese Science Bulletin2013，58，24： | 1 |
| 15 | A Com- parative Study on the Air-Side Performance of Wavy Fin- and-~be Heat Exchanger With Punched Delta Winglets in Staggered and in-Line Arrangements 显示文摘 | TIAN Liting HE Yaling TAO Yubing | 2009 | International Journal of Thermal Sciences2009，48，17： | 1 |
| 16 | A comparative study on the air-side performance of wavy fin-and-tube heat exchanger with punched delta winglets in staggered and in-line arrangements显示文摘 | Liting Tian Yaling He Yubing Tao Wenquan Tao | 2009 | International Journal of Thermal Sciences2009，，9： | 1 |
| 17 | A comparative study on the air-side performance of wavy fin-and-tube heat exchanger with punched delta winglets in staggered and in-line arrangements 显示文摘 | Tian Liting He Yaling Tao Yubing | 2009 | International Journal of Thermal Sciences2009，48，9： | 1 |
| 18 | Numerical study on performance of molten salt phase change thermal energy storage system with enhanced tubes显示文摘 | Tao Yubing He Yaling Qu Zhiguo | 2012 | Solar Energy2012，86，5： | 1 |
| 19 | Artificial evolution of OsEPSPS through an improved dual cytosine and adenine base editor generated a novel allele conferring rice glyphosate tolerance显示文摘Exploiting novel endogenous glyphosate-tolerant alleles is highly desirable and has promising potential for weed control in rice breeding. Here,through fusions of different effective cytosine and adenine deaminases with nCas9-NG, we engineered an effective surrogate two-component composite base editing system, STCBE-2, with improved C-to-T and A-to-G base editing efficiency and expanded the editing window. Furthermore,we targeted a rice endogenous OsEPSPS gene for artificial evolution through STCBE-2-mediated near-saturated mutagenesis. After hygromycin and glyphosate selection, we identified a novel OsEPSPS allele with an Asp-213-Asn(D213N)mutation(OsEPSPS-D213N) in the predicted glyphosate-binding domain, which conferred rice plants reliable glyphosate tolerance and had not been reported or applied in rice breeding. Collectively, we developed a novel dual base editor which will be valuable for artificial evolution of important genes in crops. And the novel glyphosate-tolerant rice germplasm generated in this study will benefit weeds management in rice paddy fields. | Chen Zhang Xue Zhong Shaoya Li Lei Yan Jingying Li Yubing He Yong Lin Yangjun Zhang Lanqin Xia | 2023 | Journal of Integrative Plant Biology2023，65，9： | 1 |
| 20 | Synergistic roles of LAX1 and FZP in the development of rice sterile lemma显示文摘Rice florets are subtended by two sterile lemmas,whose origin and biological functions have not been studied extensively.Here we demonstrate that two putative transcription factors,LAX PANICLE1(LAX1)and FRIZZY PANICLE(FZP),synergistically control the development of sterile lemmas.Both LAX1 and FZP are previously known for their roles in panicle and floret development.Disruption of either LAX1 or FZP greatly reduces the number of floret development.We generated new lax1 mutants(lax1-c)using CRISPR/Cas9 gene editing technology.In addition to the expected lax panicle phenotypes,we noticed that a significant number of spikelets of lax1-c developed elongated sterile lemmas.Moreover,our characterization of lax1-RNAi plants also revealed sterile lemma phenotypes similar to lax1-c mutants.We isolated a weak allele of fzp(fzp-14)in a genetic screen for lax1–1 enhancers.The fzp-14 lax1–1 double mutants completely eliminated flower development.Interestingly,the isolated fzp-14 produced spikelets with elongated sterile lemmas.Furthermore,fzp-14 was haploid-insufficient in the lax1–1 background whereas fzp-14 heterozygous plants were indistinguishable from wild type plants.The lax1–1 fzp-14+/−also developed elongated sterile lemma as observed in lax1-c,lax1-RNAi,and fzp-14,suggesting that LAX1 and FZP synergistically control sterile lemma development. | Yidong Wang Shanshan Wei Yubing He Lang Yan Rongchen Wang Yunde Zhao | 2020 | The Crop Journal2020，8，1： | 1 |
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