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| 1 | Genome of Wild Mandarin and Domestication History of Mandarin显示文摘官员(柠檬 reticulata ) 是世界范围的最重要的柠檬庄稼之一。它的驯服被相信发生在华南,它是四几千年的官员耕作的中心之一。我们在 Nanling 区域附近收集了官员的自然野人口并且在附近栽培了 landraces。我们发现柠檬性的酸水平戏剧性地在栽培官员被减少。理解 ? 官员驯服的基因基础,我们 de novo 装配了野官员的一个草稿染色体并且分析了一套 104 个柠檬染色体。我们发现 Mangshan 官员是一种原始类型并且二个独立驯服事件发生了,分别地导致在北方和南方 Nanling 山的二组栽培官员(MD1 和 MD2 ) 。二个瓶颈和有效人口尺寸的二扩大为栽培官员的 MD1 组被识别。然而,在 MD2 组织 ? 在人口尺寸有长、连续的减少。MD1 和 MD2 官员从栽培 pummelo 种类显示出种间的基因渗入的不同模式。我们在官员的驯服期间在选择下面可能在基因在柠檬酸盐内容的规定包含了的 aconitate hydratase (ACO ) 识别了高分叉的一个区域,它是。这研究为现存野官员人口的地理起源提供具体基因证据并且使驯服和官员的进化历史清楚些。 | Lun Wang Fa He Yue Huang Jiaxian He Shuizhi Yang Jiwu Zeng Chongling Deng Xiaolin Jiang Yiwen Fang Shaohua Wen Rangwei Xu Huiwen Yu Xiaoming Yang Guangyan Zhong Chuanwu Chen Xiang Yan Changfu Zhou Hongyan Zhang Zongzhou Xie Robert M. Larkin Xiuxin Deng Qiang Xu | 2018 | Molecular Plant2018，11，8： | 14 |
| 2 | Analysis of DNA Methylation Level by Methylation-Sensitive Amplification Polymorphism in Half Smooth Tongue Sole(Cynoglossus semilaevis)Subjected to Salinity Stress显示文摘Increasingly arisen environmental constraints may contribute to heritable phenotypic variation including methylation changes,which can help the animals with development,growth and survival.In this study,we assessed the DNA methylation levels in three tissues(gonad,kidney and gill) of half smooth tongue sole under the salinity stress.The methylation-sensitive amplification polymorphism(MSAP) technique was applied to illustrate the regulation of epigenetic mechanism in environmental stimuli.Fish were subjected to 15 salinity treatment for 7 and 60 days,respectively.A total of 11259 fragments were amplified with 8 pairs of selective primers.The levels of methylated DNA in different tissues of females and males without salinity stress were analyzed,which were 32.76% and 47.32% in gonad;38.13% and 37.69% in kidney;37.58% and 34.96% in gill,respectively.In addition,the significant difference was observed in gonad between females and males,indicating that discrepant regulation in gonadal development and differentiation may involve sex-related genes.Further analysis showed that total and hemi-methylation were significantly decreased under 15 salinity for 7 days,probably resulting in up-regulating salt-tolerance genes expression to adjust salt changing.With the adjustment for 60 days,total and hemi-methylation prominently went back to its normal levels to obtain equilibrium.Particularly,full methylation levels were steady along with salinity stress to maintain the stability of gene expression.Additionally,the data showed that gonads in females and gills in males were superior in adaptability.As a result,DNA methylation regulates tissue-specific epiloci,and may respond to salinity stress by regulating gene expression to maintain animal survival and activity. | LI Siping HE Feng WEN Haishen LI Jifang SI Yufeng LIU Mingyuan HE Huiwen HUANG Zhengju | 2017 | Journal of Ocean University of China2017，16，2： | 4 |
| 3 | The Biocontrol Effects of the Bacillus licheniformis W10 Strain and Its Antifungal Protein Against Brown Rot in Peach显示文摘The biocontrol effects of Bacillus licheniformis W10 bacterial suspension and its antifungal protein on peach brown rot caused by Monilinia fructicola in storage peach fruits and the effects on fruit quality were investigated. The results showed that the fruit disease suppression of B. licheniformis W10 bacterial suspension and antifungal protein were significantly higher than that of the control. Inoculation of bacterial suspension and antifungal protein prior to M. fructicola gave a better biocontrol effect, and the higher concentrations of bacterial(1 × 1010 cfu · m L-1) and antifungal protein(3.0 mg · m L-1) performed better control effects. The environmental conditions, such as temperature and humidity, affected biocontrol effects of W10 bacterial suspension and antifungal protein. The influence of environment conditions on the activity of antifungal protein was less than that on bacterial suspension. Moreover, lower temperature(4 ℃) and relative humidity(RH 70%–75%) were favorable to prevent peach brown rot by W10 bacterial suspension and its antifungal protein. The W10 bacterial suspension and antifungal protein amended with calcium [0.1% Ca(NO3)2] could enhance the biocontrol effects, and obviously put off the occurrence of peach brown rot. In addition, the bacterial suspension and antifungal protein significantly reduced the natural decay rates of peach fruits during storage, and the effects were equal to carbendazim. Moreover, both W10 bacterial suspension and antifungal protein treatments did not have effects on external and internal fruit appearance, such as chromatic aberration parameter L* of flesh, flesh firmness, soluble solids content and weight loss. Therefore, the B. licheniformis W10 is a potential biocontrol factor for peach brown rot. | JI Zhaolin HE Huiwen ZHOU Huijuan HAN Feng TONG Yunhui YE Zhengwen XU Jingyou | 2015 | Horticultural Plant Journal2015，1，3： | 4 |
| 4 | Overvoltage and Insulation Coordination for Valve Winding of ±800 kV UHVDC Converter Transformer显示文摘 | ZHOU Peihong DAI Ming HE Huiwen | 2012 | 高电压技术2012，38，12： | 4 |
| 5 | Progress of power field effect transistor based on ultra-wide bandgap Ga_2O_3 semiconductor material显示文摘As a promising ultra-wide bandgap semiconductor, gallium oxide(Ga_2O_3) has attracted increasing attention in recent years. The high theoretical breakdown electrical field(8 MV/cm), ultra-wide bandgap(~ 4.8 eV) and large Baliga's figure of merit(BFOM) of Ga_2O_3 make it a potential candidate material for next generation high-power electronics, including diode and field effect transistor(FET). In this paper, we introduce the basic physical properties of Ga_2O_3 single crystal, and review the recent research process of Ga_2O_3 based field effect transistors. Furthermore, various structures of FETs have been summarized and compared, and the potential of Ga_2O_3 is preliminary revealed. Finally, the prospect of the Ga_2O_3 based FET for power electronics application is analyzed. | Hang Dong Huiwen Xue Qiming He Yuan Qin Guangzhong Jian Shibing Long Ming Liu | 2019 | Journal of Semiconductors2019，40，1： | 4 |
| 6 | Calculation and Analysis of Switching Overvoltage Front Time and Switching Impulse Insulation Coordination for UHV AC Transmission Line of Chinese Pilot Project and Double-circuit Project显示文摘 | ZHOU Peihong HE Huiwen | 2014 | 高电压技术2014，40，6： | 2 |
| 7 | Rectanglingpanoramic images via warping 显示文摘 | He Kaiming Chang Huiwen and Sun Jian | 2013 | ACM Transactionson Graphics (S0730-0301)2013，32，4： | 1 |
| 8 | Rectangling pano- ramic images via warping 显示文摘 | He Kaiming Chang Huiwen Sun Jian | 2013 | ACM Transactions on Graphics2013，32，4： | 1 |
| 9 | DNA Methylation of pacap Gene Is Involved in Growth Superiority of Female Half Smooth Tongue Sole(Cynoglossus semilaevis)in Different Salinities显示文摘Pituitary adenylate cyclase activating polypeptide(PACAP)and growth hormone-releasing hormone(GHRH)play important roles in the GH/IGF growth axis in fishes.To determine whether epigenetic change is involved in the regulation of pacap and ghrh responses to low salinity stress in Cynoglossus semilaevis,the correlation between growth traits,DNA methylation status and gene expression level in low salinity(15,S15)and optimal salinity(30,S30)at day 7(D7)and day 60(D60)were analyzed.Results showed that exposure to low salinity for 60 days attenuated C.semilaevis growth rate.Under low salinity,DNA methylation level of pacap promoter increased in females and decreased in males at day 7,but inverted at day 60.Additionally,pacap expression was up-regulated in both males and females.The pacap promoter methylation level was higher and expression level was lower in female than in male.The results suggest that pacap promoter methylation level is negatively correlated to mRNA level and positively correlated to body weight,while gene expression level is negatively related with body weight.With the decrease of salinity,DNA methylation level of ghrh promoter and exon1,as well as its gene expression displayed minor changes.Overall,pacap gene seems to play an important role in fish growth,contributing to female growth superiority,while ghrh gene seems not pertinent under salinity stress.The results indicate that low salinity potentially affects fish growth through regulating DNA methylation in pacap promoter.This study expands the understanding of the molecular mechanism of how salinity modulates fish growth from the epigenetic perspective. | SI Yufeng HE Feng WEN Haishen LI Siping HE Huiwen | 2021 | Journal of Ocean University of China2021，20，1： | 0 |
| 10 | Magnetic-conductive bi-gradient structure design of CP/PGFF/Fe 3 O 4 composites for highly absorb e d EMI shielding and balanced mechanical strength显示文摘Developing excellent absorption-dominant electromagnetic interference(EMI)shielding composites is an urgent demand for the rapid development of 5 G technology and electronic equipment.Herein,a simple strategy is employed to fabricate carbon nanotubes-polypropylene fibers(CP)/polypropylene-glass fibers felt(PGFF)/Fe 3 O 4 composites with superior EMI shielding effectiveness and low reflection due to the magnetic-conductive bi-gradient structure which is naturally formed by deposition during the vacuum-assisted filtration process.The difference in dimensionality between one-dimensional CNT with outstand-ing electrical conductivity and zero-dimensional magnetic Fe 3 O 4 nanoparticles is the theoretical basis for the successful construction of the magnetic-conductive bi-gradient structure in a gap-rich PGFF matrix that endows the composites with“absorb-reflect-reabsorb”EMI shieldingmechanism.Whentheelectro-magnetic waves are incident from the magnetic layer,the EMI shielding effectiveness(SE)reaches 48.9 dB as the weight percentage of the conductive layer increases,more importantly,the reflection coefficients are reduced by more than 0.32 compared with that of another incident pattern.What’s more,the re-sultant composites exhibit an outstanding signal shielding function in the application.This work paves a convenient pathway for designing a magnetic-conductive bi-gradient structure and efficient absorbing EMI shielding composites applied in the next-generated electronic information and communication field. | Qiang Peng Meng Ma Si Chen Yanqin Shi Huiwen He Xu Wang | 2023 | Journal of Materials Science & Technology2023，，2： | 0 |
| 11 | LPS adsorption and inflammation alleviation by polymyxin B-modified liposomes for atherosclerosis treatment显示文摘Chronic inflammation is critical in the onset and progression of atherosclerosis(AS).The lipopolysaccharide(LPS)level in the circulation system is elevated in AS patients and animal models,which is correlated with the severity of AS.Inspired by the underlying mechanism that LPS could drive the polarization of macrophages toward the M1 phenotype,aggravate inflammation,and ultimately contribute to the exacerbation of AS,LPS in the circulation system was supposed to be the therapeutic target for AS treatment.In the present study,polymyxin(PMB)covalently conjugated to PEGylated liposomes(PLPs)were formulated to adsorb LPS through specific interactions between PMB and LPS.In vitro,the experiments demonstrated that PLPs could adsorb LPS,reduce the polarization of macrophages to M1 phenotype and inhibit the formation of foam cells.In vivo,the study revealed that PLPs treatment reduced the serum levels of LPS and pro-inflammatory cytokines,decreased the proportion of M1-type macrophages in AS plaque,stabilized AS plaque,and downsized the plaque burdens in arteries,which eventually attenuated the progression of AS.Our study highlighted LPS in the circulation system as the therapeutic target for AS and provided an alternative strategy for AS treatment. | Huiwen Liu Honglan Wang Qiyu Li Yiwei Wang Ying He Xuejing Li Chunyan Sun Onder Ergonul Fusun Can Zhiqing Pang Bo Zhang Yu Hu | 2023 | Acta Pharmaceutica Sinica B2023，13，9： | 0 |
| 12 | Progress in Research Methods on “Fluid-Diagenesis” Interactions of Fractures in Tight Reservoirs显示文摘1 Introduction Fractures in tight reservoirs have always attracted general petroleum geologists’attentions due to their importance in the processes of hydrocarbon enrichment and oil-gas exploration.Fractures can not only act as main pathways of hydrocarbon migration and fluid flow,but also provide major reservoir spaces for | WANG Xintao CHEN Yong ZHOU Yaoqi YUE Huiwen HE Chuan | 2015 | Acta Geologica Sinica(English Edition)2015，89，A01： | 0 |
| 13 | Multiple model predictive control of perching maneuver based on guardian maps显示文摘Considering the strong nonlinearity of Unmanned Aerial Vehicles(UAVs)resulting from high Angle of Attack(AOA)and fast maneuvering,we present a multi-model predictive control strategy for UAV maneuvering,which has a small amount of online calculation.Firstly,we divide the maneuver envelope of UAV into several sub-regions on the basis of the gap metric theory.A novel algorithm is then developed to determine the ploytopic model for each sub-region.According to this,a Robust Model Predictive Control based on the Idea of Comprehensive optimization(ICE-RMPC)is proposed.The control law is designed offline and optimized online to reduce the computational expense.Then,the ICE-RMPC method is applied to design the controllers of sub-regions.In addition,to guarantee the stability of whole closed-loop system,a multi-model switching control strategy based on guardian maps is put forward.Finally,the tracking performance of proposed control strategy is demonstrated by an illustrative example. | Rui CAO Huiwen WAN Zhen HE Yuping LU | 2022 | Chinese Journal of Aeronautics2022，35，5： | 0 |
| 14 | Development of an integrated process for the production of high-purityγ-aminobutyric acid from fermentation broth显示文摘γ-Aminobutyric acid(GABA),a natural non-protein amino acid,plays an irreplaceable role in regulating the life activities of organisms.Nowadays,the separation and purification of food-grade GABA from fermentation broth is still a great challenge.This research utilized monosodium glutamate as a substrate for the production of high-purity GABA via an integrated process incorporating fermentation,purification,and crystallization.Firstly,147 g·L^(-1) GABA with a yield of 99.8%was achieved through fed-batch fermentation by Lactobacillus brevis CE701.Secondly,three integrated purification methods by ethanol precipitation were compared,and crude GABA with a purity of 89,85%was obtained by the optimized method.Thirdly,GABA crystals with a purity of 98.69%and a yield of 60%were further obtained through a designed crystallization process.Furthermore,the GABA industrial production process model was established by Superproper Designer V10 software,and material balance and economic analysis were carried out.Ethanol used in the process was recovered with a recovery of 98.79%through Aspen simulated extractive distillation.Then the fixed investment(equipment purchase and installation costs)for an annual production of 80 t GABA will be about 833000 USD;the total annual production cost(raw material cost and utility cost)will be about 641000 USD.The annual sale of GABA may be at the range of 2400000-4000000 USD and the payback period will be about 1-2 year.This integrated process provides a potential way for the industrial-scale production of food-grade GABA. | Zhaofeng Zhang Juanjuan Ding Min Wu Bochao Liu Huiwen Song Shengping You Wei Qi Rongxin Su Zhimin He | 2022 | Chinese Journal of Chemical Engineering2022，，10： | 0 |
| 15 | Structural Variation between the Western and Eastern Kuqa Fold-and-thrust Belt,China:Insights from Seismic Interpretation and Analog Modeling显示文摘The Kuqa fold-and-thrust belt exhibits apparent structural variation in the western and eastern zone.Two salt layer act as effective decollements and influence the varied deformation.In this study,detailed seismic interpretations and analog modeling are presented to construct the suprasalt and subsalt structures in the transfer zone of the middle Kuqa and investigate the influence of the two salt layers.The results reveal that the relationship of the two salt layers changes from separated to connected,and then overlapped toward the foreland in the transfer zone.Different structural models are formed in the suprasalt and subsalt units due to the interaction of the two salt layers.The imbricate thrust faults form two broom-like fault systems in the subsalt units.The suprasalt units develop detached folds terminating toward the east in the region near the orogenic belt.Whereas,two offset anticlines with different trends develop at the frontal edge of the lower salt layer and the trailing edge of the upper salt layer,respectively.According to exploration results in this region,the relationship between suprasalt and subsalt structures has an influence on hydrocarbon accumulation.We believe that the connected deformation contains high-risk plays while the decoupled deformation contains well-preserved plays. | WANG Wei XIE Huiwen YIN Hongwei JIA Dong XU Zhenping LUO Haoyu HE Wanhui YUAN Rui DUAN Yunjiang | 2023 | Acta Geologica Sinica(English Edition)2023，97，4： | 0 |
| 16 | Diversity,community structure,and quantity of eukaryotic phytoplankton revealed using 18S rRNA and plastid 16S rRNA genes and pigment markers:a case study of the Pearl River Estuary显示文摘Understanding consistencies and discrepancies in characterizing diversity and quantity of phytoplankton is essential for better modeling ecosystem change.In this study,eukaryotic phytoplankton in the Pearl River Estuary,South China Sea were investigated using nuclear 18S rRNA and plastid 16S or 23S rRNA genes and pigment analysis.It was found that 18S abundance poorly explained the variations in total chlorophyll a(Chl-a).However,the ratios of log-transformed 18S abundance to Chl-a in the major phytoplankton groups were generally environment dependent,suggesting that the ratio has potential as an indicator of the physiological state of phytoplankton.The richness of 18S-based operational taxonomic units was positively correlated with the richness of 16S-based amplicon sequence variants of the whole phytoplankton community,but insignifcant or weak for individual phytoplankton groups.Overall,the 18S based,rather than the 16S based,community structure had a greater similarity to pigment-based estimations.Relative to the pigment data,the proportion of haptophytes in the 18S dataset,and diatoms and cryptophytes in the 16S dataset,were underestimated.This study highlights that 18S metabarcoding tends to refect biomass-based community organization of eukaryotic phytoplankton.Because there were lower copy numbers of plastid 16S than 18S per genome,metabarcoding of 16S probably approximates cell abundance-based community organization.Changes in biomass organization of the pigment-based community were sensitive to environmental changes.Taken together,multiple methodologies are recommended to be applied to more accurately profle the diversity and community composition of phytoplankton in natural ecosystems. | Shumin Xu Guihao Li Cui He Yi Huang Dan Yu Huiwen Deng Zhuyin Tong Yichong Wang Christine Dupuy Bangqin Huang Zhuo Shen Jie Xu Jun Gong | 2023 | Marine Life Science & Technology2023，5，3： | 0 |
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