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您的检索式：作者名="HE HongQiu"
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| 1 | Development of a high-throughput assay for the HIV-1 integrase disintegration reaction显示文摘Both HIV-1 integrase (IN) and the central catalytic domain of IN (IN-CCD) catalyze the disintegration reaction in vitro.In this study,IN and IN-CCD proteins were expressed and purified,and a high-throughput format enzyme-linked immunosorbent assay (ELISA) was developed for the disintegration reaction.IN exhibited a marked preference for Mn2+ over Mg2+ as the divalent cation cofactor in disintegration.Baicalein,a known IN inhibitor,was found to be an IN-CCD inhibitor.The assay is sensitive and specific for the study of disintegration reaction as well as for the in vitro identification of antiviral drugs targeting IN,especially targeting IN-CCD. | HE HongQiu,LIU Bin,ZHANG XiaoYi,CHEN WeiZu & WANG CunXin College of Life Science and Bioengineering,Beijing University of Technology,Beijing 100124,China | 2010 | Science China(Life Sciences)2010，53，2： | 7 |
| 2 | Time-resolved fluoroimmunoassay for quantitative determination of tylosin and tilmicosin in edible animal tissues显示文摘To quantitatively determine tylosin and tilmicosin in edible animal tissues,a time-resolved fluoroimmunoassay(TRFIA) has been developed and validated.For this purpose,desmycosin-O-carboxymethoxylamine-BSA was fixed onto microtiter plates,standards and samples were loaded and,finally,diluted europium-labeled anti-tylosin antibodies were added.Results show that the limit of detection for tylosin was 0.03 ng mL-1 and that for tilmicosin was 0.05 ng mL-1.The recoveries were 73.6% to 120.5%,with coefficients of variation below 15.6% in various biological matrices spiked with tylosin and tilmicosin at concentrations of 50-200 ngg-1.There was good correlation(R2>0.99) between the TRFIA,an enzyme-linked immunosorbent assay and high performance liquid chromatography data.In conclusion,the new TRFIA is applicable to the detection of tylosin and tilmicosin and is an effective and economical method that will enable high-throughput sample screening.The method is expected to be widely applicable. | WEI Shu LE Tao CHEN Yong XU Jian HE HongQiu NIU XiaoDong LUO JinHua | 2013 | Chinese Science Bulletin2013，58，15： | 3 |
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