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| 1 | Development of SnAg-based lead-free solders in electronics packaging显示文摘 | ZHANG Liang HE Chengwen GUO Yonghuan | 2012 | Microelectronics Reliability2012，52，3： | 1 |
| 2 | Effect of Zn on properties and microstructure of SnAgCu alloy显示文摘 | Zhang Liang Han Jiguang He Chengwen | 2012 | Journal of Ma- terials Science: Materials in Electronics2012，23，11： | 1 |
| 3 | An Immersed-Boundary Method Based on the Gas Kinetic BGK Scheme for Incompressible Viscous Flow 显示文摘 | YUAN Ruifeng ZHONG Chengwen ZHANG He | 2015 | Journal of Computational Physics2015，296，： | 1 |
| 4 | Clinical value of assessing serum levels of inflammatory cytokines in the early diagnosis of patients with primary liver carcinoma:a retrospective observational study显示文摘Objective:To identify potential early diagnostic markers for hepatitis B progression to primary liver carcinoma using routine immunological tests based on 6 cytokine combinations.Methods:Eight hundred and ninety-nine patients with hepatitis B progressing to early primary liver carcinoma admitted to and treated at Changhai Hospital,Naval Military Medical University,Shanghai,China between March 2015 and June 2017 were included in this observational study,including 666 patients with HBsAg^(+),HBeAb^(+),HBcAb^(+)liver carcinoma and 233 patients with HBsAg+,HBeAg+,HBcAb+liver carcinoma.Receiver operating characteristic(ROC)curves were used to evaluate the efficiency of the different cytokine in the diagnosis of hepatocellular carcinoma in patients with hepatitis B.This study was approved by the Institutional Review Board of Changhai Hospital,Naval Military Medical University,China(approval No.CHEC2020-080)on June 6,2020.Results:Changed levels of interleukin(IL)-1β,IL-2R,IL-8,and tumor necrosis factor(TNF)-a were statistically significant(P<0.05).The area under the ROC curve,sensitivity,specificity,positive predictive value,negative predictive value,and Youden index for the diagnosis of primary liver carcinoma using the combination of IL-1β,IL-2R,IL-8,and TNF-α were 0.938,79.2%,96.7%,96%,82.0%,0.759,respectively.The serum alpha-fetoprotein level in patients with primary liver carcinoma was positively correlated with IL-2R(r=0.3502,P<0.001),IL-8(r=0.1558,P=0.0273),and TNF-α(r=0.2544,P<0.001)levels.The equation fitted to the results was logit(P)=0.086+0.01IL-2R–0.001IL-8–0.033TNF-α–0.041IL-1β.Conclusion:Our study establishes a novel,potentially valuable diagnostic model based on four cytokines related to the early stages of liver carcinoma. | Chengwen He Qin Wei Jun Zhu Qin Qin Huaizhou Wang Shanrong Liu | 2021 | Journal of Bio-X Research2021，4，1： | 1 |
| 5 | 人胆囊癌耐砷细胞株的建立及其凋亡相关基因差异表达检测(英文)显示文摘Objective:The aim of the study was to establish an arsenic trioxide(ATO)-resistant cell line of human gallbladder carcinoma,GBC-SD/ATO,and to analyze the differential expressions of its apoptosis-associated genes,so as to investigate a correlation between ATO induced resistance of gallbladder carcinoma and expressions of apoptosis associated genes.Methods:The resistant cell line was obtained in vitro by culture of human gallbladder carcinoma cell line GBC-SD with increasingly stepwise concentrations of ATO.The sensitivities of GBC-SD cells and GBC-SD/ATO cells to ATO were determined by MTT assay respectively.cDNA microarray containing 458 apoptosis-related human genes was used to compare the gene expression profiles of GBC-SD/ATO cells and corresponding sensitive cell line GBC-SD.Results:GBC-SD/ATO cell line was established successfully after 8 months of exposure to increasing concentrations of ATO.Compared with the parental cell line,GBC-SD/ATO was 13.6 times more resistant to ATO.Of the 458 apoptosis-related genes,17 genes were detected having > 2-fold difference of expression between the GBC-SD/ATO and GBC-SD cells,with 6 genes up-regulated and 11 genes down-regulated in GBC-SD/ATO cells.Conclusion:The 17 genes involved in the apoptosis pathway might be relevant to the resistance of GBC-SD/ATO cells to ATO,suggesting that the modulation of expression of apoptosis-related genes may be a main mechanism of acquired resistance in GBC-SD/ATO. | Dalong Wu Xiaowei He Chengwen Zhang | 2010 | The Chinese-German Journal of Clinical Oncology2010，9，2： | 1 |
| 6 | Adding power of artificial intelligence to situational awareness of large interconnections dominated by inverter‐based resources显示文摘Large‐scale power systems exhibit more complex dynamics due to the increasing inte-gration of inverter‐based resources(IBRs).Therefore,there is an urgent need to enhance the situational awareness capability for better monitoring and control of power grids dominated by IBRs.As a pioneering Wide‐Area Measurement System,FNET/GridEye has developed and implemented various advanced applications based on the collected synchrophasor measurements to enhance the situational awareness capability of large‐scale power grids.This study provides an overview of the latest progress of FNET/GridEye.The sensors,communication,and data servers are upgraded to handle ultra‐high density synchrophasor and point‐on‐wave data to monitor system dynamics with more details.More importantly,several artificial intelligence(AI)‐based advanced appli-cations are introduced,including AI‐based inertia estimation,AI‐based disturbance size and location estimation,AI‐based system stability assessment,and AI‐based data authentication. | Lin Zhu Yinfeng Zhao Yi Cui Shutang You Wenpeng Yu Shengyuan Liu He Yin Chang Chen Yuru Wu Wei Qiu Mirka Mandich Hongyu Li Adedasola Ademola Chengwen Zhang Chujie Zeng Xinlan Jia Weikang Wang Haoyu Yuan Huaiguang Jiang Jin Tan Yilu Liu | 2021 | High Voltage2021，6，6： | 0 |
| 7 | A 66 amino acid micro-peptide encoded by long non-coding RNA RP11-119F7.5 was identified in hepatocellular carcinoma显示文摘Objective: Emerging data have shown that non-coding RNAs (ncRNAs) can encode micro-peptides (≤100 amino acids) that play an important role in regulating physiological and pathological processes. Herein, we explored ncRNAs that may encode micro-peptides that are involved in the development of hepatocellular carcinoma (HCC).Methods: High-throughput sequencing of ribosomal protein S6 (RPS6) was performed in four cancer cell lines using RNA-immunoprecipitation (RIP). UCSC databases obtained the full length of the gene sequences and quantitative polymerase chain reaction (qPCR) was used to evaluate expression levels of ncRNAs of interest. The coding activity of ncRNA was assessedin vitro by co-immunoprecipitation, plasmid transfection, western blot, immunofluorescence and RNA fluorescencein situ hybridization. Mass spectrometry was performed to explore the potential functions of candidate micro-peptide in HCC. This study involving human tissue specimens was conducted in accordance withDeclaration of Helsinki and approved by the Institutional Review Board of Changhai Hospital, Naval Military Medical University, China (approval No. CHEC2020-081) on June 6, 2020.Results: We performed RIP assay using primary antibodies for RPS6 and high-throughput sequencing. A total of 223 overlapping genes were captured by RPS6-RIP. Venn diagram analysis revealed that 60 overlapping genes were detected in four cancer cell lines. QRT-PCR showed that six of the candidate genes (RP11-298J20.4, RP11-4O1.2, RP11-119F7.5, RP11-448G15.3, HCP5, RP11-517B11.7) were expressed in Huh7 and Hep3B cells. Further analysis of these six candidate genes and found that five (RP11-298J20.4, RP11-4O1.2, RP11-119F7.5, RP11-448G15.3, RP11-517B11.7) displayed higher expression levels in HCC cell lines (Huh7, Hep3B) and tumor tissues than in liver cell lines (L-02, QSG-7701) and non-tumor tissues, respectively. Performed additional RIP assays and confirmed that four of the genes (RP11-4O1.2, RP11-119F7.5, RP11-448G15.3, RP11-517B11 .7) bound RPS6. We obtained the full length of the four gene sequences from the UCSC database and analyzed the open reading frames by ORF Finder;to determine the translation potential of the four candidate small open reading frames (smORFs), we subcloned a FLAG epitope tag into the C-terminal of the four selected smORFs before the stop codon, and the fusion sequences were then cloned into three different plasmid vectors (pSPT19, pcDNA3.1, and PEGFP-N1). We performed coupled transcription and translation reactions and found that the pSPT19 plasmids encoded small peptidesin vitro. After then transfected the pcDNA3.1 constructs into Huh7 cells, and a single 7.2 kDa micro-peptide was encoded from the candidate smORF of RP11.119F7.5. We transfected the recombinant pEGFP-N1 plasmids with smORFs in HCC cells, and western blot analysis revealed a band above GFP in the RP11.119F7.5 recombinant plasmid lane. The coding potential of the RP11-119F7.5 vector was also confirmed by immunofluorescence assay. Fluorescencein situ hybridization assay revealed that RP11-119F7.5 was localized in the cytoplasm and nucleoplasm of HCC cells. Gene ontology enrichment analysis showed that the micro-peptide–interacting proteins were mainly involved in extracellular exosomes. We also found the identified proteins were involved in several biological functions like protein binding, poly(A) RNA binding, translational initiation, and the nuclear-transcribed mRNA catabolic process. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis showed the peptide-interacting proteins might participate in several critical pathways including ribosome, biosynthesis of amino acids, carbon metabolism, biosynthesis of antibiotics, glycolysis and gluconeogenesis, pathogenicEscherichia coli infection and influenza A.Conclusion: Our study revealed a novel micro-peptide translated by ncRNA RP11-119F7.5, highlighting the coding ability and potential role of ncRNAs in HCC. | Chengwen He Zhiyong Liu Yanan Pang Yin Jia Qin Qin Ruijiao Kong Hui Zhang Shanrong Liu | 2022 | Journal of Bio-X Research2022，5，4： | 0 |
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