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| 1 | High-throughput screening of mouse gene knockouts identifies established and novel skeletal phenotypes显示文摘Screening gene function in vivo is a powerful approach to discover novel drug targets. We present high-throughput screening(HTS) data for 3 762 distinct global gene knockout(KO) mouse lines with viable adult homozygous mice generated using either gene-trap or homologous recombination technologies. Bone mass was determined from DEXA scans of male and female mice at 14 weeks of age and by microCT analyses of bones from male mice at 16 weeks of age. Wild-type(WT) cagemates/littermates were examined for each gene KO. Lethality was observed in an additional 850 KO lines. Since primary HTS are susceptible to false positive findings, additional cohorts of mice from KO lines with intriguing HTS bone data were examined. Aging,ovariectomy, histomorphometry and bone strength studies were performed and possible non-skeletal phenotypes were explored. Together, these screens identified multiple genes affecting bone mass: 23 previously reported genes(Calcr, Cebpb, Crtap, Dcstamp, Dkk1, Duoxa2, Enpp1, Fgf23, Kiss1/Kiss1 r, Kl(Klotho),Lrp5, Mstn, Neo1, Npr2, Ostm1, Postn, Sfrp4, Slc30a5, Slc39a13, Sost, Sumf1, Src, Wnt10b), five novel genes extensively characterized(Cldn18, Fam20 c, Lrrk1, Sgpl1, Wnt16), five novel genes with preliminary characterization(Agpat2, Rassf5, Slc10a7, Slc26a7, Slc30a10) and three novel undisclosed genes coding for potential osteoporosis drug targets. | Robert Brommage Jeff Liu Gwenn M Hansen Laura L Kirkpatrick David G Potter Arthur T Ss Brian Zambrowicz David R Powell Peter Vogel | 2014 | Bone Research2014，2，3： | 7 |
| 2 | NOTUM inhibition increases endocortical bone formation and bone strength显示文摘The disability,mortality and costs caused by non-vertebral osteoporotic fractures are enormous.Existing osteoporosis therapies are highly effective at reducing vertebral but not non-vertebral fractures.Cortical bone is a major determinant of non-vertebral bone strength.To identify novel osteoporosis drug targets,we phenotyped cortical bone of 3 366 viable mouse strains with global knockouts of druggable genes.Cortical bone thickness was substantially elevated in Notum?/?mice.NOTUM is a secreted WNT lipase and we observed high NOTUM expression in cortical bone and osteoblasts but not osteoclasts.Three orally active small molecules and a neutralizing antibody inhibiting NOTUM lipase activity were developed.They increased cortical bone thickness and strength at multiple skeletal sites in both gonadal intact and ovariectomized rodents by stimulating endocortical bone formation.Thus,inhibition of NOTUM activity is a potential novel anabolic therapy for strengthening cortical bone and preventing non-vertebral fractures. | Robert Brommage Jeff Liu Peter Voge Faika Mseeh Andrea Y.Thompson David G.Potter Melanie K.Shadoan Gwenn M.Hansen Sabrina Jeter-Jones Jie Cui Dawn Bright Jennifer P.Bardenhagen Deon D.Doree Sofia Moverare-Skrtic Karin H.Nilsson Petra Henning Ulf H.Lerner Claes Ohlsson Arthur T.Sands James E.Tarver David R.Powell Brian Zambrowicz Qingyun Liu | 2019 | Bone Research2019，7，1： | 2 |
| 3 | Inexpensive laser cooling and trapping experiment for undergraduate laboratories 显示文摘 | CARL WIEMAN GWENN FLOWERS SARAH GILBERT | 1995 | Am J Phys1995，63，： | 1 |
| 4 | Rentier states,unruly law,and the rise of opposition:the political economy of oil states显示文摘 | Okruhlik Gwenn | 1999 | Comparative Politics1999，31，3： | 1 |
| 5 | Chemical structure of cement aged at normal and elevated temperatures and pressures, Part Ⅱ: Low permeability class G oilweU cement 显示文摘 | Gwenn Le Saou Eric Lecolier Alain Rivereau | 2006 | Cement and Concrete Research2006，36，1： | 1 |
| 6 | Applications of neuroreceptor imaging to psychiatry research显示文摘 | Gwenn S Jeremy K Sara G | 2003 | Psychopharma Bul2003，37，4： | 1 |
| 7 | Risk of Lung Cancer Associated With Occupational Exposure to Mineral Wools: Updating Knowledge From a French Population-Based Case–Control Study, the ICARE Study显示文摘 | Florence Guida Sophie Paget-Bailly Farida Lamkarkach Oumar Gaye Stéphane Ducamp Gwenn Menvielle Alexandra Papadopoulos Mireille Matrat Jo?lle Févotte Sylvie Cénée Diane Cyr Annie Schmaus Matthieu Carton Loredana Rado? Bénédicte Lap?tre-Ledoux Florence Mol | 2013 | Journal of Occupational and Environmental Medicine2013，，7： | 1 |
| 8 | Influence of limestone on the hydration of Portland cements显示文摘 | Barbara Lothenbach Gwenn Le Saout Emmanuel Gallucci | 2008 | Cement and Concrete Research2008，，38： | 1 |
| 9 | Influence of limestone and anhydrite on the hydration of portland cements显示文摘 | MACIEJ Z ANNE R GWENN L S | 2014 | Cement and Concrete Composites2014，46，： | 1 |
| 10 | Chemical structure of cement aged at normal and elevated temperatures and pressures, Part II: Low permeability class G oilwell cement 显示文摘 | Gwenn Le Saout Eric Lecolier Alain Rivereau | 2006 | Cement and concrete research2006，36，3： | 1 |
| 11 | Association Between Carotid Plaque Characteristics and Subsequent Ischemic Cerebrovascular Events: A Prospective Assessment With MRI—Initial Results显示文摘 | Norihide Takaya Chun Yuan Baocheng Chu Tobias Saam Hunter Underhill Jianming Cai Nam Tran Nayak L. Polissar Carol Isaac Marina S. Ferguson Gwenn A. Garden Steven C. Cramer Kenneth R. Maravilla Beverly Hashimoto Thomas S. Hatsukami | 2006 | Stroke2006，，3： | 1 |
| 12 | Influence of limestone on the hydration of Port-land cements显示文摘 | Barbara Lothenbach Gwenn Le Saout Emmanuel Gallucci Karen Scrivener | 2008 | Cement and Concrete Research2008，38，： | 1 |
| 13 | Pediatrics显示文摘 | Gwenn Schurgin O'Keeffe Kathleen Clarke-Pearson:The Impact of Social Media on Children Adolescents and Familie | 2011 | (4):800-8042011，，4： | 1 |
| 14 | Applications of neuroreceptor imaging to psychiatry research显示文摘 | Gwenn S Jeremy K Sara G | 2003 | Psychopharma Bul2003，37，： | 1 |
| 15 | Influence of limestone on the hydration of Portland cements显示文摘 | Barbara Lothenbach Gwenn Le Saout Emmanuel Gallucci Karen Scrivener | 2008 | Cement and Concrete Research2008，，6： | 1 |
| 16 | Impact of chloride on the mineralogy of hydrated Portland cement systems显示文摘 | Magdalena Balonis Barbara Lothenbach Gwenn Le Saout | 2010 | Cement and Concrete Research2010，40，7： | 1 |
| 17 | Therapeutic plasma exchange in the treatment of exertional heat stroke and multiorgan failure显示文摘 | Vimal Master Sankar Raj Amanda Alladin Brent Pfeiffer Chryso Katsoufis Marissa Defreitas Alicia Edwards-Richards Jayanthi Chandar Wacharee Seeherunvong Gwenn McLaughlin Gaston Zilleruelo Carolyn L. Abitbol | 2013 | Pediatric Nephrology2013，，6： | 1 |
| 18 | Model study of the enzymatic modification of natural extracts : Peroxidase-Based removal of eugenol from rose essential Oil显示文摘 | Charfeddine Bouhlel Gwenn'Ann Dolhem Xavier Fernandez | 2012 | J Agri Food Chem2012，60，： | 1 |
| 19 | Microglia Biology in Health and Disease显示文摘 | Gwenn A. Garden Thomas M?ller | 2006 | Journal of Neuroimmune Pharmacology2006，，2： | 1 |
| 20 | In-home ac- tivity reeognition:Bayesian inference for hidden Markov models 显示文摘 | FRANCISCO J O GWENN E PAULA D T | 2014 | IEEE Pervasive Computing2014，13，3： | 1 |
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