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| 1 | Transplantation of collagen scaffold with autologous bone marrow mononuclear cells promotes functional endometrium reconstruction via downregulating ΔNp63 expression in Asherman's syndrome显示文摘Asherman's syndrome(AS) is a common disease that presents endometrial regeneration disorder. However, little is known about its molecular features of this aregenerative endometrium in AS and how to reconstruct the functioning endometrium for the patients with AS. Here, we report that ΔNp63 is significantly upregulated in residual epithelial cells of the impaired endometrium in AS; the upregulated-ΔNp63 induces endometrial quiescence and alteration of stemness. Importantly, we demonstrate that engrafting high density of autologous bone marrow mononuclear cells(BMNCs) loaded in collagen scaffold onto the uterine lining of patients with AS downregulates ΔNp63 expression, reverses ΔNp63-induced pathological changes, normalizes the stemness alterations and restores endometrial regeneration. Finally, five patients achieved successful pregnancies and live births. Therefore, we conclude that ΔNp63 is a crucial therapeutic target for AS. This novel treatment significantly improves the outcome for the patients with severe AS. | Guangfeng Zhao Yun Cao Xianghong Zhu Xiaoqiu Tang Lijun Ding Haixiang Sun Juan Li Xinan Li Chenyan Dai Tong Ru Hui Zhu Jingjie Lu Caimei Lin Jingmei Wang Guijun Yan Huiyan Wang Lei Wang Yimin Dai Bin Wang Ruotian Li Jianwu Dai Yan Zhou Yali Hu | 2017 | Science China(Life Sciences)2017，60，4： | 39 |
| 2 | Correlation and Significance of Midkine and Estrogen Receptor Beta Protein Expression in Non-Small Cell Lung Cancer显示文摘OBJECTIVE Midkine (MK),a new member of the heparin-binding growth factor family,was found recently to have a highexpression level in many carcinoma specimens,including thoseof the esophagus,gall bladder,pancreas,colorectum,breast andlung.Estrogen receptor beta (ER-β),a recently cloned estrogenreceptor subtype,was also found to be highly expressed in lungtumor tissue,in contrast to a lower level of expression in normallung tissue.However,few relevant studies on these proteins havebeen published.The aims of our study were to investigate theexpression of midkine and ER-β proteins in non-small cell lungcancer (NSCLC) and to examine the relationship between theirexpression and the clinicopathologic data as well as to analyse thecorrelation of their expression in NSCLC.METHODS By immunohistochemistry,MK and ER-β were ex-amined in 24 surgically resected cases of NSCLC with their corre-sponding paraneoplastic and normal lung tissues.RESULTS MK and ER-β were overexpressed in NSCLC.Thelevels of MK and ER-β expression in NSCLC were found to be sig-nificantly negatively correlated with the pathological classification(P=0.042 and 0.021,respectively),and their expression decreasedwith a raise in the classification.Spearman's correlation analysisshowed that the correlation of their expression in NSCLC wasstrong (correlation coefficient[r_s]= 0.535,P=0.007<0.01).CONCLUSION The expression levels of MK and ER-β to someextent reflect the malignant degree of NSCLC,and their combineddetection may be of great value in early diagnosis,treatments ofpatients with NSCLC and can predict the prognoses. | Shihua Zhang Guangfeng Zhao Qingling Wang Kaihua Lu Yayi Hou | 2008 | Chinese Journal of Clinical Oncology2008，5，6： | 2 |
| 3 | Placenta-derived IL-32β activates neutrophils to promote preeclampsia development显示文摘Immune activation at the maternal-fetal interface is a main pathogenic factor of preeclampsia(PE).Neutrophils(PMNs)are activated in PE patients,but the mechanism and consequences of PMN activation need to be further explored.Here,we demonstrated that interleukin-32(IL-32)expression was significantly upregulated in syncytiotrophoblasts(STBs)and that IL-32β was the major isoform with increased expression in the placenta of severe PE(sPE)patients.Furthermore,the level of IL-32 expression in the placenta was correlated with its level in the serum of sPE patients,indicating that IL-32 in the serum is derived mainly from the placenta.Then,in vitro experiments showed that IL-32β could highly activate PMNs and that these IL-32β-activated PMNs were better able to adhere to endothelial cells(HUVECs)and enhance the expression of vascular cell adhesion molecule-1(VCAM-1)and intercellular cell adhesion molecule-1(ICAM-1)in HUVECs,which could be reversed by preincubation with the NADPH oxidase inhibitor VAS 2870.In addition,we showed that IL-32β mainly activated PMNs by binding to proteinase 3.Finally,IL-32β administration induced a PE-like phenotype in a pregnant mouse model.This study provides evidence of the involvement of IL-32β in the pathogenesis of PE. | Dan Liu Qiang Li Hailin Ding Guangfeng Zhao Zhiyin Wang Chenrui Cao Yimin Dai Mingming Zheng Xiangyu Zhu Qianwen Wu Ya Wang Honglei Duan Huirong Tang Xianyan Lu Yayi Hou Yali Hu | 2021 | Cellular & Molecular Immunology2021，18，4： | 2 |
| 4 | DNA/Poly(p-Aminobenzensulfonic Acid)Composite Bi-Layer Modified Glassy Carbon Electrode for Determination of Dopamine and Uric Acid Under Coexistence of Ascorbic Acid显示文摘 | Lin Xiangqin Kang Guangfeng Lu Liping | 2007 | Bioelec-trochemistry2007，70，2： | 1 |
| 5 | DNA/poly(p-aminobenzensulfonic acid)composite bi-layer modified glassy carbon electrode for determination of dopamine and uric acid under coexistence of ascorbic acid 显示文摘 | LIN Xiangqin KANG Guangfeng LU Liping | 2007 | Bioelectrochemistry2007，70，2： | 1 |
| 6 | DNA/Poly(p - aminobenzensulfonic acid) composite bi -layer modified glassy carbon electrode for determination of dopamine and uric acid under coexistence of ascorbic acid显示文摘 | LIN Xiangqin KANG Guangfeng LU Liping | 2007 | Bioelectrochemistry2007，70，： | 1 |
| 7 | Synthesis of nanosized tungsten powder显示文摘Nanosized tungsten powder was synthesized by means of different methods and under different conditions with nanosized WO3 powder. The powder and the intermediate products were characterized using XRD, SEM, TEM, BET (Brunauer Emmett Teller Procedure) and SAXS (X-ray diffracto-spectrometer/Kratky small angle scattering goniometer). The results show that nanosized WO3 can be completely reduced to WO2 at 600℃ after 40 min, and WO2 can be reduced to W at 700℃ after 90 min, moreover, the mean size of W particles is less than 40 nm. Furthermore, the process of WO3→WO2→W excelled that of WO3→W in getting stable nanosized tungsten powder with less grain size. | Guangfeng Lu Junjie Hao Zhimeng Guo Ruizhu Zhang Ji Luo Liying Zhang | 2005 | Journal of University of Science and Technology Beijing2005，12，4： | 1 |
| 8 | Effect of Balanced Application of Potash Fertilizer on the Yield of Rice Yongyou 15 and Soil Nutrient Content显示文摘By the field experiment,we set up the treatments of non-application of potash fertilizer,conventional fertilization,balanced fertilization and halved application of potash fertilizer,and studied the effect of different treatments on rice yield and soil nutrient content. The results showed that compared with non-application of potash fertilizer,conventional fertilization and balanced fertilization significantly increased the spike length,thousand kernel weight,yield and total potassium content of the stalk,as well as soil organic matter,total nitrogen and available phosphorus content; compared with conventional fertilization,the balanced fertilization did not significantly increase the rice growth and yield,but effectively increased rice grain total nitrogen,stalk total nitrogen and total phosphorus content,as well as soil available phosphorus and available potassium content. It could be found that potash fertilizer played a certain role in increasing rice yield and soil nutrient content,and the balanced fertilization had the most significant effect. | Xiaoxia LIU Zhe ZHANG Guangfeng WANG Lingli LU Junjun WANG Yiding CHEN | 2017 | Asian Agricultural Research2017，9，3： | 1 |
| 9 | Epidemiology and vector efficiency during a dengue fever outbreak in Cixi, Zhejiang Province, China显示文摘 | Yang Tianci Lu Liang Fu Guiming Zhong Shi Ding Gangqiang Xu Rong Zhu Guangfeng Shi Nanfeng Fan Feilong Liu Qiyong | 2009 | Journal of Vector Ecology2009，，1： | 1 |
| 10 | Comparative Analysis of the Photosynthetic Characteristics and Active Compounds of Semiliquidambar cathayensis Chang Heteromorphic Leaves显示文摘In the present study,the variation patterns of leaf shape in different populations of individual Semiliquidambar cathayensis plants were analyzed to investigate the relationship among leaf shape variation,photosynthetic properties,and active compounds to understand the genetic characteristics of S.cathayensis and screen elite germplasms.The leaf shape of 18 offspring from three natural S.cathayensis populations was analyzed to investigate the level of diversity and variation patterns of leaf shape.Furthermore,photosynthetic pigment content,physiological parameters of photosynthesis,and the active compounds in leaves of different shapes were determined.Statistical analysis showed that the leaf shape variation in S.cathayensis indicated a high level of genetic diversity among and within the populations.Cluster analysis showed that the three natural populations formed two clusters,one whose offspring was dominated by entire leaves and another characterized by palmately trifoliate leaves.The differences in photosynthetic characteristics and active compounds of leaves of three different shapes were comprehensively evaluated using principal component analysis.Two principal components with a cumulative contribution rate of 92.768%were extracted,of which the highest comprehensive score was for asymmetrically lobed leaves.The leaf shape in different S.cathayensis germplasms exhibited distinct patterns,and there were some correlations between the photosynthetic properties and active compounds in leaves of different shapes.Thus,the leaf shape can be used to predict active compound content,and in turn,select varieties based on that purpose;it also provides a simple and effective method to classify S.cathayensis germplasms. | Xiaoming Tian Guangfeng Xiang Hao Lv Jing Peng Lu Zhu | 2023 | Phyton-International Journal of Experimental Botany2023，92，3： | 0 |
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