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| 1 | Ultrasound-triggered noninvasive regulation of blood glucose levels using microgels integrated with insulin nanocapsules显示文摘Diabetes is a serious public health problem affecting 422 million people worldwide. Traditional diabetes management often requires multiple daily insulin injections, associated with pain and inadequate glycemia control. Herein, we have developed an ultrasound-triggered insulin delivery system capable of pulsatile insulin release that can provide both long-term sustained and fast on-demand responses. In this system, insulin-loaded poly(lactic-co-glycolic acid) (PLGA) nanocapsules are encapsulated within chitosan microgels. The encapsulated insulin in nanocapsules can passively diffuse from the nanoparticle but remain restricted within the microgel. Upon ultrasound treatment, the stored insulin in microgels can be rapidly released to regulate blood glucose levels. In a chemically-induced type 1 diabetic mouse model, we demonstrated that this system, when activated by 30 s ultrasound administration, could effectively achieve glycemic control for up to one week in a noninvasive, localized, and pulsatile manner. | Jin Di Jicheng Yu Qun Wang Shanshan Yao Yanqi Ye Matthew Pless Yong Zhu Yun Jing Zhen Gu | 2017 | Nano Research2017，10，4： | 6 |
| 2 | Effect of Jianpiyiqi granule on ocular myasthenia gravis in children prepared with a formula from Traditional Chinese Medicine显示文摘OBJECTIVE: To investigate the effect of Jianpiyiqi granule, prepared according to a Traditional Chinese Medicine(TCM) formula, on the ocular type of myasthenia gravis(MG) caused by a defect in synaptic transmission at the neuromuscular junction.METHODS: A total of 155 children with ocular MG were recruited from January 2008 to January 2015.All individuals received ineffective glucocorticoid treatment prior to admission, and were given Jianpiyiqi granules(two doses per day) for 12 months.Plasma levels of acetylcholine receptor autoantibodies(ACh R-Ab), cytokines, immune parameters and clinical score were analyzed.RESULTS: After intervention with Jianpiyiqi granule for 12 months, the levels of cytokines [including interleukin(IL)-6, IL-12, IL-17], immune parameters[including immunoglobulin G(Ig G), Ig M, free triiodothyronine, free thyroxine] and ACh R-Ab were sig-nificantly decreased(P = 0.042, P = 0.049, P = 0.011,P = 0.02, P = 0.04, P = 0.03, P = 0.01; F = 21.60, P =0.000). In contrast, IL-4 levels were significantly increased(P = 0.049). The absolute clinical score after treatment declined significantly compared with before treatment(F = 33.24, P = 0.000). The effective cure rate and the total effective rate gradually increased to a maximum of 149(96.2%) and 151(97.4%) in MG patients after 12 months of treatment, respectively.CONCLUSION: The Jianpiyiqi granule treatment lowered ACh R-Ab levels and improved cytokine and other immune parameter levels, which suggests that the granule could be an ancillary treatment for ocular MG in children caused by a defect in synaptic transmission at the neuromuscular junction. | Qi Guoyan Liu Peng Gu Shanshan Yang Hongxia Dong Huimin Xue Yinping | 2017 | Journal of Traditional Chinese Medicine2017，37，4： | 4 |
| 3 | Dynamic cell transition and immune response landscapes of axolotl limb regeneration revealed by single-cell analysis显示文摘Dear Editor,The axolotl,Ambystoma mexicanum,has extraordinary capability to fully recover multiple tissues after lost,whereas such capability has disappeared in mammals.Thus,deci-phering detailed mechanisms underlying axolotl regenera-tion could provide valuable lessons for regenerative medicine.However,many questions,such as the origin of essential progenitor cells and key responses of individual types of cells for regeneration remain elusive(Haas and Whited,2017).Newly developed single-cell RNA sequenc-ing(scRNA-seq)method enables researchers to observe cellular and molecular dynamics in axolotl regeneration at the single-cell resolution(Gerber et al.,2018;Leigh et al.,2018),but the reported transcriptome landscapes are only for certain cell types or in certain regenerative stages.A complete overview of the regeneration process for all cell types is still lacking. | Hanbo Li Xiaoyu Wei Li Zhou Weiqi Zhang Chen Wang Yang Guo Denghui Li Jjianyang Chen Tianbin Liu Yingying Zhang Shuai Ma Congyan Wang Fujian Tan Jiangshan Xu Yang Liu Yue Yuan Liang Chen Qiaoran Wang Jing Qu Yue Shen Shanshan Liu Guangyi Fan Longqi Liu Xin Liu Yong Hou Guang-Hui Liu Ying Gu Xun Xu | 2021 | Protein & Cell2021，12，1： | 3 |
| 4 | Basic fibroblast growth factor protects against influenza A virus-induced acute lung injury by recruiting neutrophils显示文摘Influenza virus (IAV)infection is a major cause of severe respiratory illness that affects almost every country in the world.IAV infections result in respiratory illness and even acute lung injury and death,but the underlying mechanisms responsible for IAV pathogenesis have not yet been fully elucidated.In this study,the basic fibroblast growth factor 2 (FGF2)level was markedly increased in H1N1 virus-infected humans and mice.FGF2,which is predominately derived from epithelial cells,recruits and activates neutrophils via the FGFR2-PI3K-AKT-NFKB signaling pathway.FGF2 depletion or knockout exacerbated influenzaassociated disease by impairing neutrophil recruitment and activation.More importantly,administration of the recombinant FGF2 protein significantly aUeviated the severity of IAV-induced lung injury and promoted the survival of IAV-infected mice.Based on the results from experiments in which neutrophils were depleted and adoptively transferred,FGF2 protected mice against IAV , infection by recruiting neutrophils.Thus,FGF2 plays a critical role in preventing IAV-induced lung injury,and FGF2 is a promising potential therapeutic target during IAV infection. | Keyu Wang Chengcai Lai Tieling Li Cheng Wang Wei Wang Bing Ni Changqing Bai Shaogeng Zhang Lina Han Hongjing Gu Zhongpeng Zhao Yueqiang Duan Xiaolan Yang Li Xing Lingna Zhao Shanshan Zhou Min Xia Chengyu Jiang Xiliang Wang Penghui Yang | 2018 | Journal of Molecular Cell Biology2018，10，6： | 3 |
| 5 | Expression profile of circular RNAs in myocardial ischemia/reperfusion with and without intermittent hypobaric hypoxia preconditioning显示文摘Dear Editor,Myocardial injury following ischemia/reperfusion(I/R)is a common clinical scenario in patients suffering from ischemic heart disease(Hausenloy et al.,2016).The involvement of proteins and signaling pathways is well studied in myocardial I/R injury and protection(Hausenloy et al.,2016;Yang et al.,2012),however,more than 98%of the transcriptional output in mammals are noncoding RNAs(ncRNAs).These products range from short microRNAs(miRNAs)to long noncoding RNAs(lncRNAs,Das et al.,2018)and modulate critical pathophysiologic mechanisms,such as apoptosis,oxidative stress,Ca2+overload and inflammation,during myocardial I/R injury and protection(Das et al.,2018). | Jiliang Tan Shanshan Gu Yanjun Zheng Huangtian Yang | 2019 | Science China(Life Sciences)2019，62，8： | 3 |
| 6 | Effects of Olanzapine–Fluoxetine Combination Treatment of Major Depressive Disorders on the Quality of Life During Acute Treatment Period显示文摘 | Wei Qu Shanshan Gu Han Luo Qianying Tang Junwei Guo | 2014 | Cell Biochemistry and Biophysics2014，，3： | 1 |
| 7 | Hepatitis B virus X protein promotes hepatoma cell invasion and metastasis by stabilizing S nail protein显示文摘 | Haiou Liu Le Xu Hongyong He Yu Zhu Jing Liu Shanshan Wang Lin Chen Qian Wu Jiejie Xu Jianxin Gu | 2012 | Cancer Sci2012，，12： | 1 |
| 8 | A constantinterest risk model with tax payments 显示文摘 | Wang Shanshan Zhang Chunsheng Wang Gu@ng | 2010 | Stochastic Models2010，26，： | 1 |
| 9 | Evolution and roles of cytokinin genes in angiosperms 2:Do ancient CKXs play housekeeping roles while non-ancient CKXs play regulatory roles?显示文摘Cytokinin oxidase/dehydrogenase(CKX)is a key enzyme responsible for the degradation of endogenous cytokinins.However,the origins and roles of CKX genes in angiosperm evolution remain unclear.Based on comprehensive bioinformatic and transgenic plant analyses,we demonstrate that the CKXs of land plants most likely originated from an ancient chlamydial endosymbiont during primary endosymbiosis.We refer to the CKXs retaining evolutionarily ancient characteristics as“ancient CKXs”and those that have expanded and functionally diverged in angiosperms as“non-ancient CKXs”.We show that the expression of some non-ancient CKXs is rapidly inducible within 15 min upon the dehydration of Arabidopsis,while the ancient CKX(AtCKX7)is not drought responsive.Tobacco plants overexpressing a non-ancient CKX display improved oxidative and drought tolerance and root growth.Previous mutant studies have shown that non-ancient CKXs regulate organ development,particularly that of flowers.Furthermore,ancient CKXs preferentially degrade cis-zeatin(cZ)-type cytokinins,while non-ancient CKXs preferentially target N6-(Δ2-isopentenyl)adenines(iPs)and trans-zeatins(tZs).Based on the results of this work,an accompanying study(Wang et al.http://gffzzd3cc09b8251d45dfskfqnnw0opowb6v00.ffgz.tsg.suse.edu.cn/10.1038/s41438-019-0211-x)and previous studies,we hypothesize that non-ancient CKXs and their preferred substrates of iP/tZ-type cytokinins regulate angiosperm organ development and environmental stress responses,while ancient CKXs and their preferred substrates of cZs play a housekeeping role,which echoes the conclusions and hypothesis described in the accompanying report(Wang,X.et al.Evolution and roles of cytokinin genes in angiosperms 1:Doancient IPTs play housekeeping while non-ancient IPTs play regulatory roles?Hortic Res 7,(2020).http://gffzzd3cc09b8251d45dfskfqnnw0opowb6v00.ffgz.tsg.suse.edu.cn/10.1038/s41438-019-0211-x). | Xiaojing Wang Jing Ding Shanshan Lin Decai Liu Tingting Gu HanWu Robert N.Trigiano Richard McAvoy Jinling Huang Yi Li | 2020 | Horticulture Research2020，7，1： | 1 |
| 10 | Study on the relationship between working memory and EFL listening comprehension显示文摘 | Gu Shanshan Wang Tongshun | | 0，，06： | 1 |
| 11 | Discovery of spirooxindole-ferrocene hybrids as novel MDM2 inhibitors显示文摘A series of spirooxindole-ferrocene hybrids bearing five or four contiguous chiral centers were designed and synthesized via organocatalysis.In vitro protein binding and cellular proliferation assays suggested that compound 5 d was the most potent mouse double minute 2 homolog(MDM2)inhibitor.In addition,mechanistic studies indicated that compound 5 d suppressed MDM2-mediated p53 degradation,induced apoptosis and promoted oxidative damage.Molecular docking studies have suggested that 5 d binds to MDM2 by mimicking the Trp23 and Leu26 residues of p53.This work can provide a basis for the development of novel multifunctional MDM2 inhibitors.The further exploration of more derivatives from this library and additional investigation of organocatalysis application in the development of new molecules may generate new potential lead compounds for cancer-targeted therapy. | Jun Mu Xin Xie Shanshan Xiong Yuehua Zhang Yuting Wang Qian Zhao Hongping Zhu Wei Huang Gu He | 2021 | Chinese Chemical Letters2021，32，6： | 1 |
| 12 | Preferential growth of long ZnO nanowires and its application in gas sensor显示文摘 | Cuiping Gu Li Shanshan Jiarui Huang Chengcheng Shi Jinhuai Liu | 2013 | Sensors & Actuators: B. Chemical2013，，： | 1 |
| 13 | miR-96-5p regulates myocardial infarction-induced cardiac fibrosis via Smad7/Smad3 pathway显示文摘Fibrotic remodelling contributes to heart failure in myocardial infarction.MicroRNAs(miRNAs)play a crucial role in myocardial fibrosis.However,current antifibrotic therapeutic strategies using miRNAs are far from effective.In this study,we aim to investigate the effect of miR-96-5p on cardiac fibrosis.Our work reveals a significant upregulation of miR-96-5p level in the ventricular tissues of myocardial infarction mice,as well as in neonatal rat cardiac fibroblasts stimulated with TGF-βor Ang II as shown by qPCR assay.In myocardial infarction mice,miR-96-5p knockdown using antagomir alleviates the aggravated cardiac fibrosis and exacerbated myocardial function caused by myocardial infarction surgery as shown by the echocardiography and Masson’s staining analysis.In contrast,immunofluorescence staining results reveal that miR-96-5p overexpression in neonatal rat cardiac fibroblasts contributes to an increase in the expressions of fibrosis-associated genes and promotes the proliferation and differentiation of cardiac fibroblasts.Conversely,miR-96-5p downregulation using inhibitor presents adverse consequences.Furthermore,Smad7 expression is downregulated in fibrotic cardiac tissues,and the Smad7 gene is identified as a direct target of miR-96-5p by dual luciferase assay.Indeed,Smad7 knockdown weakens the antifibrotic effect of the miR-96-5p inhibitor on cardiac fibroblasts.Moreover,Smad3 phosphorylation is elevated in fibrotic cardiac tissues,and interestingly,the Smad3 inhibitor suppresses the profibrotic effect of the miR-96-5p mimic.Taken together,our findings demonstrate that the Smad7/Smad3 signaling pathway mediates the profibrotic effect of miR-96-5p in cardiac fibrosis. | Huanyu Gu Yi Duan Shanshan Li Qin Wang Wen Zhen Wei Zhang Yingying Zhang Min Jiang Chun Wang | 2022 | Acta Biochimica et Biophysica Sinica2022，54，12： | 1 |
| 14 | Metal-organic framework-derived carbon nanotubes with multi-active Fe-N/Fe sites as a bifunctional electrocatalyst for zinc-air battery显示文摘Sustainable metal-air batteries demand high-efficiency,environmentally-friendly,and non-precious metal-based electrocatalysts with bifunctionality for both the oxygen reduction reaction(ORR)and oxygen evolution reaction(OER).In this research,novel functional carbon nanotubes with multi-active sites including well-dispersed single-atom iron throughout the walls and encapsulated ultrafine iron nanoparticles were synthesized as an electrocatalyst(FeNP@Fe-N-C)through one-step pyrolysis of metal-organic frameworks.High-resolution synchrotron powder X-ray diffraction and X-ray absorption spectroscopy were applied to characterize the unique structure of the electrocatalyst.In comparison to the commercial Pt/C and Ru O_(2)electrodes,the newly prepared FeNP@Fe-N-C presented a superb bifunctional performance with its narrow potential difference(Egap)of 0.73 V,which is ascribed to the metallic Fe nanoparticles that boosts the adsorption and activation of oxygen on the active sites with an enhanced O_(2)adsorption capacity of 7.88 cm^(3)g^(-1)and synergistically functionalizes the iron atoms dispersed on the nanotubes.A rechargeable zinc-air battery based on FeNP@Fe-N-C exhibited a superior open-circuit voltage(1.45 V),power density(106.5 m W cm^(-2)),and stable cycling performance.The green technique developed in this work for the fabrication of functional nanotubes raises the prospect of making more efficient electrocatalysts for sustainable energy cells. | Chao Yang Shanshan Shang Qinfen Gu Jin Shang Xiao-yan Li | 2022 | Journal of Energy Chemistry2022，31，3： | 1 |
| 15 | Effect of dual targeting procyanidins nanoparticles on metabolomics of lipopolysaccharide-stimulated inflammatory macrophages显示文摘Inflammation plays an important role in the occurrence and development of many inflammatory diseases.The purpose of this study was to evaluate the anti-inflammatory effect and metabolic behavior of the dual targeting procyanidins(PC)nanoparticles on lipopolysaccharide(LPS)-stimulated inflammatory macrophages by metabolomics method.The double-targeting PC nanoparticles could specifi cally target both the CD44 receptor and mitochondria,while the single targeting PC-loaded nanoparticles that could target the CD44 receptor on the surface of macrophages.The double-targeting PC nanoparticles had better inhibitory effect than single-targeting PC nanoparticles on the leakage of lactate dehydrogenase and reactive oxygen species overexpression induced by LPS.Amino acid metabolism,energy metabolism and purine metabolism were disordered in LPS-treated group,and metabolic pathway analysis indicated that the double-targeting PC nanoparticles reversed some of LPS impacts.The changes of these potential biomarkers and their corresponding pathways are helpful to further understand the mechanism of PC nanoparticles in alleviating inflammation,and promote their application in nutrition intervention. | Shanshan Tie Lijuan Zhang Bin Li Shanghua Xing Haitao Wang Yannan Chen Weina Cui Shaobin Gu Mingqian Tan | 2023 | Food Science and Human Wellness2023，12，6： | 1 |
| 16 | A new portable open-path instrument for ambient NH_(3) and on-road emission measurements显示文摘Increased attentions to vehicle emission of NH_(3)have been paid since it is generally regarded as an important source in urban areas.Here,we developed a movable instrument based on Differential Optical Absorption Spectroscopy(DOAS)principle for detecting on-road NH_(3),which can avoid the losses in the sampling process attributed to the non-sampling methods.For this mobile DOAS,the temporal resolution,detection limit and relative error for NH_(3)were 1 min,2.29 ppbv and 4.57%±2.44%,respectively.By employed to the on-road measurements along the arterial highway in Shanghai,the spatial distributions of NH_(3)and NO were obtained,and their dependence of traffic and road conditions were studied.The slopes of linear regression between NH_(3)and NO were 0.40,0.02 and 0.07 on the Middle Ring Road,Outer Ring Road and Chongming Island Ring Road.It indicates that light gasoline vehicles(LGVs)were found to be the main contributor to NH_(3)emissions,while heavy-duty diesel vehicles(HDVs)mainly emitted NO.Based on the measured NH_(3)in the tunnel,the mileagebased NH_(3)emission factor per vehicle was estimated to be 17.9±6.3 mg/km.The reported open-path instrument can be broadly used in on-road pollutant monitoring or vehicle emissions,and the measurements can reveal the real situation of emission characteristics,even find the abnormal operations of vehicle catalyst system. | Wenhao Dai Shanshan Wang Sanbao Zhang Jian Zhu Chuanqi Gu Zhibin Sun Ruibin Xue Bin Zhou | 2024 | Journal of Environmental Sciences2024，，2： | 0 |
| 17 | TRIM34 attenuates colon inflammation and tumorigenesis by sustaining barrier integrity显示文摘Loss of the colonic inner mucus layer leads to spontaneously severe colitis and colorectal cancer.However,key host factors that may control the generation of the inner mucus layer are rarely reported.Here,we identify a novel function of TRIM34 in goblet cells(GCs)in controlling inner mucus layer generation.Upon DSS treatment,TRIM34 deficiency led to a reduction in Muc2 secretion by GCs and subsequent defects in the inner mucus layer.This outcome rendered TRIM34-deficient mice more susceptible to DSS-induced colitis and colitis-associated colorectal cancer.Mechanistic experiments demonstrated that TRIM34 controlled TLR signaling-induced Nox/Duox-dependent ROS synthesis,thereby promoting the compound exocytosis of Muc2 by colonic GCs that were exposed to bacterial TLR ligands.Clinical analysis revealed that TRIM34 levels in patient samples were correlated with the outcome of ulcerative colitis(UC)and the prognosis of rectal adenocarcinoma.This study indicates that TRIM34 expression in GCs plays an essential role in generating the inner mucus layer and preventing excessive colon inflammation and tumorigenesis. | Qiaoshi Lian Shanshan Yan Qi Yin Chenghua Yan Wanwei Zheng Wangpeng Gu Xinhao Zhao Weiguo Fan Xuezhen Li Liyan Ma Zhiyang Ling Yaguang Zhang Jie Liu Jinsong Li Bing Sun | 2021 | Cellular & Molecular Immunology2021，18，2： | 0 |
| 18 | Hepatocyte growth factor protects pulmonary endothelial barrier against oxidative stress and mitochondria-dependent apoptosis显示文摘Background:Pulmonary microvascular endothelial cells(PMVECs)were not complex,and the endothelial barrier was destroyed in the pathogenesis progress of acute lung injury(ALI)/acute respiratory distress syndrome(ARDS).Previous studies have demonstrated that hepatocyte growth factor(HGF),which was secreted by bone marrow mesenchymal stem cells,could decrease endothelial apoptosis.We investigated whether mTOR/STAT3 signaling acted in HGF protective effects against oxidative stress and mitochondria-dependent apoptosis in lipopolysaccharide(LPS)-induced endothelial barrier dysfunction and ALI mice.Methods:In our current study,we introduced LPS-induced PMEVCs with HGF treatment.To investigate the effects of mammalian target of rapamycin(mTOR)/signal transducer and activator of transcription 3(STAT3)pathway in endothelial oxidative stress and mitochondria-dependent apoptosis,mTOR inhibitor rapamycin and STAT3 inhibitor S3I-201 were,respectively,used to inhibit mTOR/STAT3 signaling.Moreover,lentivirus vector-mediatedmTORC1(Raptor)andmTORC2(Rictor)gene knockdown modifications were introduced to evaluatemTORC1 andmTORC1 pathways.Calcium measurement,reactive oxygen species(ROS)production,mitochondrial membrane potential and protein,cell proliferation,apoptosis,and endothelial junction protein were detected to evaluate HGF effects.Moreover,we used the ALI mouse model to observe the mitochondria pathological changes with an electron microscopein vivo.Results:Our study demonstrated that HGF protected the endothelium via the suppression of ROS production and intracellular calcium uptake,which lead to increased mitochondrial membrane potential(JC-1 and mitochondria tracker green detection)and specific proteins(complex I),raised anti-apoptosis Messenger Ribonucleic Acid level(B-cell lymphoma 2 and Bcl-xL),and increased endothelial junction proteins(VE-cadherin and occludin).Reversely,mTOR inhibitor rapamycin and STAT3 inhibitor S3I-201 could raise oxidative stress and mitochondria-dependent apoptosis even with HGF treatment in LPS-induced endothelial cells.Similarly,mTORC1 as well as mTORC2 have the same protective effects in mitochondria damage and apoptosis.Inin vivo experiments of ALI mouse,HGF also increased mitochondria structural integrity via the mTOR/STAT3 pathway.Conclusion:In all,these reveal that mTOR/STAT3 signaling mediates the HGF suppression effects to oxidative level,mitochondria-dependent apoptosis,and endothelial junction protein in ARDS,contributing to the pulmonary endothelial survival and barrier integrity. | Shanshan Meng Feiping Xia Jingyuan Xu Xiwen Zhang Ming Xue Mingyuan Gu Fengmei Guo Yingzi Huang Haibo Qiu Yi Yang | 2022 | Chinese Medical Journal2022，，7： | 0 |
| 19 | Toplayer-dependent crystallographic orientation imaging in the bilayer two-dimensional materials with transverse shear microscopy显示文摘Nanocontact properties of two-dimensional(2D)materials are closely dependent on their unique nanomechanical systems,such as the number of atomic layers and the supporting substrate.Here,we report a direct observation of toplayer-dependent crystallographic orientation imaging of 2D materials with the transverse shear microscopy(TSM).Three typical nanomechanical systems,MoS_(2) on the amorphous SiO_(2)/Si,graphene on the amorphous SiO_(2)/Si,and MoS_(2) on the crystallized Al_(2)O_(3),have been investigated in detail.This experimental observation reveals that puckering behaviour mainly occurs on the top layer of 2D materials,which is attributed to its direct contact adhesion with the AFM tip.Furthermore,the result of crystallographic orientation imaging of MoS_(2)/SiO_(2)/Si and MoS_(2)/Al_(2)O_(3) indicated that the underlying crystalline substrates almost do not contribute to the puckering effect of 2D materials.Our work directly revealed the top layer dependent puckering properties of 2D material,and demonstrate the general applications of TSM in the bilayer 2D systems. | Sabir Hussain Rui Xu Kunqi Xu Le Lei Shuya Xing Jianfeng Guo Haoyu Dong Adeel Liaqat Rashid Iqbal Muhammad Ahsan Iqbal Shangzhi Gu Feiyue Cao Yan Jun Li Yasuhiro Sugawara Fei Pang Wei Ji Liming Xie Shanshan Chen Zhihai Cheng | 2021 | Frontiers of physics2021，16，5： | 0 |
| 20 | RASGRP1 targeted by H3K27me3 regulates myoblast proliferation and differentiation in mice and pigs显示文摘Skeletal muscle is not only the largest organ in the body that is responsible for locomotion and exercise but also crucial for maintaining the body’s energy metabolism and endocrine secretion.The trimethylation of histone H3 lysine 27(H3K27me3)is one of the most important histone modifications that participates in muscle development regulation by repressing the transcription of genes.Previous studies indicate that the RASGRP1 gene is regulated by H3K27me3 in embryonic muscle development in pigs,but its function and regulatory role in myogenesis are still unclear.In this study,we verify the crucial role of H3K27me3 in RASGRP1 regulation.The gain/loss function of RASGRP1 in myogenesis regulation is performed using mouse myoblast C2C12 cells and primarily isolated porcine skeletal muscle satellite cells(PSCs).The results of qPCR,western blot analysis,EdU staining,CCK-8 assay and immunofluorescence staining show that overexpression ofRASGRP1 promotes cell proliferation and differentiation in both skeletal muscle cell models,while knockdown of RASGRP1 leads to the opposite results.These findings indicate that RASGRP1 plays an important regulatory role in myogenesis in both mice and pigs. | Liyao Xiao Jiaxin Qiao Yiyang Huang Baohua Tan Linjun Hong Zicong Li Gengyuan Cai Zhenfang Wu Enqin Zheng Shanshan Wang Ting Gu | 2024 | Acta Biochimica et Biophysica Sinica2024，56，3： | 0 |
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