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| 1 | Corn bined effects of waves and plants on a mud deposition event at a mudflat-saltmarsh edge in the Bahia Blanca estuary显示文摘 | Paula D Pratolongo Gerardo M E Perillo M Cintia Piccolo | 2010 | Estuarine Coastal and Shelf Science2010，87，： | 1 |
| 2 | Effects of food availability and age on the reproductive effort of the apple snail,Pomacea canaliculata(Lamarck)(Gastropoda:Ampullariidae)显示文摘 | GERARDO F E JR YOICHI Y TAKASHI W | 2002 | Applied Entomology and Zoology2002，37，4： | 1 |
| 3 | Technology, Trade, and Wage Inequality in Mexico before and after NAFTA 显示文摘 | Gerardo E | 2003 | Journal of Development Economics2003，72，2： | 1 |
| 4 | Analysis anddesign of direct power control (DPC) for a three phase synchronous rectifier via output regulation subspaces显示文摘 | Gerardo E Aleksandar M S Juan M C | 2003 | IEEE Transactions on Power Electronics2003，18，3： | 1 |
| 5 | High-strength Mg-Zn-Y alloys produced by powder metallurgy 显示文摘 | Mora E Gerardo Garc6s Onorbe E | 2009 | Scripta Materialia2009，60，9： | 1 |
| 6 | HP0953-hypothetical virulence factor overexpresion and localization during Helicobacter pylori infection of gastric epithelium显示文摘BACKGROUND The high prevalence and persistence of Helicobacter pylori(H. pylori) infection, as well as the diversity of pathologies related to it, suggest that the virulence factors used by this microorganism are varied. Moreover, as its proteome contains 340hypothetical proteins, it is important to investigate them to completely understand the mechanisms of its virulence and survival. We have previously reported that the hypothetical protein HP0953 is overexpressed during the first hours of adhesion to inert surfaces, under stress conditions, suggesting its role in the environmental survival of this bacterium and perhaps as a virulence factor.AIM To investigate the expression and localization of HP0953 during adhesion to an inert surface and against gastric(AGS) cells.METHODS Expression analysis was performed for HP0953 during H. pylori adhesion. HP0953 expression at 0,3, 12, 24, and 48 h was evaluated and compared using the Kruskal-Wallis equality-of-populations rank test. Recombinant protein was produced and used to obtain polyclonal antibodies for immunolocalization. Immunogold technique was performed on bacterial sections during adherence to inert surfaces and AGS cells, which was analyzed by transmission electron microscopy. HP0953 protein sequence was analyzed to predict the presence of a signal peptide and transmembrane helices, both provided by the ExPASy platform, and using the GLYCOPP platform for glycosylation sites. Different programs, via, I-TASSER, RaptorX, and HHalign-Kbest, were used to perform three-dimensional modeling.RESULTS HP0953 exhibited its maximum expression at 12 h of infection in gastric epithelium cells.Immunogold technique revealed HP0953 localization in the cytoplasm and accumulation in some peripheral areas of the bacterial body, with greater expression when it is close to AGS cells.Bioinformatics analysis revealed the presence of a signal peptide that interacts with the transmembrane region and then allows the release of the protein to the external environment. The programs also showed a similarity with the Tip-alpha protein of H. pylori. Tip-alpha is an exotoxin that penetrates cells and induces tumor necrosis factor alpha production, and HP0953 could have a similar function as posttranslational modification sites were found;modifications in turn require enzymes located in eukaryotic cells. Thus, to be functional, HP0953 may necessarily need to be translocated inside the cell where it can trigger different mechanisms producing cellular damage.CONCLUSION The location of HP0953 around infected cells, the probable posttranslational modifications, and its similarity to an exotoxin suggest that this protein is a virulence factor. | Nancy K Arteaga-Resendiz Gerardo E Rodea Rosa María Ribas-Aparicio Alma L Olivares-Cervantes Juan Arturo Castelán-Vega Joséde Jesús Olivares-Trejo Sandra Mendoza-Elizalde Edgar O López-Villegas Christian Colín Pamela Aguilar-Rodea Alfonso Reyes-López Marcela Salazar García Norma Velázquez-Guadarrama | 2022 | World Journal of Gastroenterology2022，28，29： | 1 |
| 7 | An adaptive controller in stationary reference frame for D-Statcom in unbalanced operation显示文摘 | Gerardo E Aleksandar M S Paolo M | 2004 | IEEE Transactions on Industrial Electronics2004，51，2： | 1 |
| 8 | Geomorphology of a sand wave in lower Chesapeake Bay, Virginia, U S A显示文摘 | Gerardo M E Perillo John C Ludwick | 1984 | Geo-Marine Letters1984，4，2： | 1 |
| 9 | Oral insulin delivery显示文摘 | Gerardo PG Mathiowitz E | 1999 | Adv Drug Deliv Rev1999，35，： | 1 |
| 10 | Influence of tungsten on the carbon nanotubes growth by CVD process显示文摘 | MARIANO E GERARDO H R MORENO M S SILVIA G ROBERTO C | 2009 | Journal of Alloys and Compounds2009，479，12： | 1 |
| 11 | Fractal analysis of tidal channels in the Bahia Blanca estuary (Argentina) 显示文摘 | Angeles GuiUermo R Perillo Gerardo M E Cintia Piccolo M | 2004 | Geomorphology2004，57，34： | 1 |
| 12 | Global observability analysis of sensorless induction motors显示文摘 | Sebastian I R Jaime M Gerardo E P | 2004 | Automatica2004，40，： | 1 |
| 13 | Relating aviation service difficulty reports to accident data for safety trend prediction显示文摘 | Ralph R Fullwood Robert E Hall Gerardo Martinez-Guridi | 1998 | Reliability Engineering & System Safety1998，60，1： | 1 |
| 14 | Spirulina (Arthrospira) protects against valproic acid-induced neural tube defects in mice显示文摘 | GERARDO N E C NORMA P C RICARDO P P | 2012 | J Med Food2012，15，12： | 1 |
| 15 | Analysis and design of direct power control ( DPC ) for a three phase synchronous rectifier via output regulation subspaces 显示文摘 | GERARDO E ALEKSANDAR M | 2003 | IEEE Transactions on Power E- lectronics2003，18，3： | 1 |
| 16 | Association of Coronary Heart Disease Incidence with Carotid Arterial Wall Thickness and Major Risk Factors: The Atherosclerosis Risk in Communities (ARIC) Study, 1987-1993 显示文摘 | LLOYD E CHAMBLESS GERARDO HEISS AARON R FOLSOM | 1997 | Am J Epidemiol1997，146，： | 1 |
| 17 | Multiple response optimization of styrene rubber emulsion polymerization显示文摘 | Gerardo M D Alejandro O Carlos E B | 2009 | Computers and Chemical Engineering2009，33，： | 1 |
| 18 | Physics of Thermal Waves in Homogeneous and Inhomo- geneous (two-layer) Samples 显示文摘 | Gurevich Y G Logvinov G N Gerardo G Gabino E L | 2003 | International Journal of Thermal sciences2003，42，1： | 1 |
| 19 | Experimental study on performance and emissions of a high speed diesel engine fuelled with n- butanol diesel blends under premixed low temperature combustion 显示文摘 | Valentino Gerardo Corcione Felice E Iannuzzi Stefano E | 2012 | Fuel2012，92，： | 1 |
| 20 | An adaptive controller in stationary reference frame for D-station in unbalanced opetration显示文摘 | Gerardo E Aleksandar M S Paolo M | 2004 | IEEE Transactions on Industrial Electronics2004，51，2： | 1 |
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